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Protein marker of obstructive nephropathy

A technology for obstructive nephropathy and protein, applied in the field of protein markers, can solve the problems of difficult to detect, hidden changes in urinary system function, difficult early diagnosis, treatment and cure rate, etc.

Inactive Publication Date: 2016-04-20
THE INST OF BASIC MEDICAL SCI OF CHINESE ACAD OF MEDICAL SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The functional changes of the urinary system are hidden and difficult to detect. The current diagnosis method

Method used

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  • Protein marker of obstructive nephropathy
  • Protein marker of obstructive nephropathy
  • Protein marker of obstructive nephropathy

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Experimental program
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Embodiment 1

[0034] Materials and Reagents

[0035] 1) Instrument: Rat urine collection tube: purchased from BD Company. TripleTOF5600 mass spectrometer: purchased from ABI Company; Agilent1200 high performance liquid chromatograph: purchased from Agilent Company; MillliQRG ultrapure water system: purchased from Millipore Company; C18 reversed-phase analysis column (RP column, 0.1 × 150 mm, 3 μm, ): purchased from MichromBioresources.

[0036] 2) Main reagents: deionized water comes from MillliQRG ultrapure water system; chromatographic grade acetonitrile, formic acid and methanol are produced by Fisher; ammonium iodoacetamide (IAA), ammonium bicarbonate and dithiothreitol (DTT) are from Merck Purchased; sequencing-grade trypsin was purchased from Sigma; annexin A1, vimentin and moesin antibodies were all from Abcam, and α-actinin and clusterin antibodies were all from ABGENT.

[0037] 3) Animals: Male SD rats (body weight 180-200 g) were purchased from Beijing Institute of Medical Expe...

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Abstract

The invention relates to a protein marker of obstructive nephropathy, specifically to an application of human obstructive nephropathy urine differential protein obtained by unilateral ureteral ligation model in rats and mass spectrometry as a potential marker. A simpler, faster and noninvasive method and selection can be provided for detection in early diagnosis and therapeutic process of obstructive nephropathy and prognosis evaluation. Specifically, the invention relates to an application of a combination part capable of being selectively combined to one or more types of proteins in the preparation of a reagent for diagnosis of human obstructive nephropathy.

Description

technical field [0001] The invention relates to protein markers related to human obstructive nephropathy. Specifically, the present invention relates to the application of human obstructive nephropathy and disease severity-related protein markers obtained by using a rat nephropathy model with lateral ureteral ligation and mass spectrometry analysis. Background technique [0002] Significance of urinary proteomics in kidney disease research: [0003] Urine is an important source of biomarkers, and it is more likely to change than blood urine, and these changes can more sensitively reflect changes in body functions, that is, see YouheG.Canurinebethegoldmineforbiomarkerdiscovery? SciChinaLifeSci2013;43. Urine is the main output of the kidney, and it can be easily obtained in large quantities and non-invasively. It is often called in vitro renal biopsy and has important diagnostic significance in clinical practice. The functional changes of the urinary system are hidden and d...

Claims

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Application Information

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IPC IPC(8): G01N33/68
Inventor 高友鹤袁媛
Owner THE INST OF BASIC MEDICAL SCI OF CHINESE ACAD OF MEDICAL SCI
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