Scar removing composition and dressing

Abstract

The invention provides scar removing composition and a dressing comprises a mesenchymal stem cell extract, a fibroblast growth factor and an aloe extract, wherein the mass concentration of the fibroblast growth factor is 10-50 ng / mL, and the mass fraction of the aloe extract is 0.1%-1%; a mesenchymal stem cell culture solution is obtained through sequential culture of P1-P5 generations of mesenchymal stem cells with a mixed culture medium and a phenol-red-free culture medium; the mixed culture medium comprises a DMEM / F12 culture medium and fetal calf serum. The aloe extract is prepared with the following method: epidermis and mesophyll of fresh leaves of aloe vera are separated, then gel juice is squeezed from the mesophyll and sequentially sterilized, concentrated and freeze-dried, and the aloe extract is obtained. The scar removing composition adopts the mesenchymal stem cell culture solution, is low in immunogenicity and harmless to human bodies, is matched with the fibroblast growth factor and the aloe extract and can fade scars and repair skin wound surfaces.

Description

technical field

[0001] The invention belongs to the technical field of biomedicine, and in particular relates to a scar-removing composition and a dressing. Background technique

[0002] The acne scars left by puberty leave an indelible "mark of youth". With the progress of modern society, love of beauty not only represents a kind of right, but also a kind of respect for self. How to dilute this unpleasant "imprint of youth" has become a topic of concern to many beauty lovers.

[0003] Scar removal can be achieved through drugs, laser or surgery. Surgical laser methods have certain risks. Most of the drug scar removal products on the market currently use some organic macromolecular chemical products containing benzene rings or add some hormones to enhance the scar removal effect. Scar effect, such as the Chinese patent whose publication number is CN105055235A, has adopted chemical substances such as simethicone, paraben and triethanolamine, which inevitably has certain dama...

Examples

Embodiment 1

[0068] 1. Collection of Stem Cell Conditioned Medium

[0069] Take the umbilical cord mesenchymal stem cells of the P3 generation and culture them with DMEM / F12+10% FBS. When the cells are in the exponential growth phase and the confluence reaches 80%, remove the culture supernatant, wash twice with PBS, and add 0.15ml / cm 2 After the phenol red-free 1640 was cultured for 72 hours, the conditioned medium was collected, and the collected conditioned medium was centrifuged at 300 g for 5 minutes, and the supernatant was taken and passed through a 0.22 μm filter membrane to obtain the stem cell conditioned medium for future use.

[0070] 3D printed dressing

[0071] 2.3D modeling

[0072] Use the built-in laser of the 3D printer to scan the surface of the patient's scar to obtain data such as the size, shape, thickness of the wound, and the thickness of the epidermis and dermis of the patient's normal skin. Edit to make it produce a continuous surface image; use Studio4.0 softwa...

Embodiment 2

[0096] 1. Collection of stem cell conditioned medium

[0097] Take P3 generation umbilical cord mesenchymal stem cells and culture them with DMEM / F12+10% FBS. When the cells are in the exponential growth phase and the confluence reaches 80%, remove the culture supernatant, wash twice with PBS, and add 0.10ml / cm 2 After the phenol red-free 1640 was cultured for 72 hours, the conditioned medium was collected, and the collected conditioned medium was centrifuged at 200 g for 5 minutes, and the supernatant was taken, passed through a 0.22 μm filter membrane, and the resulting medium was the stem cell conditioned medium for use.

[0098] 3D printed dressing

[0099] 2. 3D modeling

[0100] Use the built-in laser of the 3D printer to scan the surface of the patient's scar to obtain data such as the size, shape, thickness of the wound, and the thickness of the epidermis and dermis of the patient's normal skin. Edit to make it produce a continuous surface image; use Studio4.0 softwa...

Embodiment 3

[0109] 1. Collection of stem cell conditioned medium

[0110] Take the umbilical cord mesenchymal stem cells of the P3 generation and culture them with DMEM / F12+10% FBS. When the cells are in the exponential growth phase and the confluence reaches 80%, remove the culture supernatant, wash twice with PBS, and add 0.2ml / cm 2 After the phenol red-free 1640 was cultured for 72 hours, the conditioned medium was collected, and the collected conditioned medium was centrifuged at 400 g for 5 minutes, and the supernatant was taken, passed through a 0.22 μm filter membrane, and the resulting medium was the stem cell conditioned medium for use.

[0111] 3D printed dressing

[0112] 2. 3D modeling

[0113] Use the built-in laser of the 3D printer to scan the surface of the patient's scar to obtain data such as the size, shape, thickness of the wound, and the thickness of the epidermis and dermis of the patient's normal skin. Edit to make it produce a continuous surface image; use Studio...

technical field

[0001] The invention belongs to the technical field of biomedicine, and in particular relates to a scar-removing composition and a dressing. Background technique

[0002] The acne scars left by puberty leave an indelible "mark of youth". With the progress of modern society, love of beauty not only represents a kind of right, but also a kind of respect for self. How to dilute this unpleasant "imprint of youth" has become a topic of concern to many beauty lovers.

[0003] Scar removal can be achieved through drugs, laser or surgery. Surgical laser methods have certain risks. Most of the drug scar removal products on the market currently use some organic macromolecular chemical products containing benzene rings or add some hormones to enhance the scar removal effect. Scar effect, such as the Chinese patent whose publication number is CN105055235A, has adopted chemical substances such as simethicone, paraben and triethanolamine, which inevitably has certain dama...

Examples

Embodiment 1

[0068] 1. Collection of Stem Cell Conditioned Medium

[0069] Take the umbilical cord mesenchymal stem cells of the P3 generation and culture them with DMEM / F12+10% FBS. When the cells are in the exponential growth phase and the confluence reaches 80%, remove the culture supernatant, wash twice with PBS, and add 0.15ml / cm 2 After the phenol red-free 1640 was cultured for 72 hours, the conditioned medium was collected, and the collected conditioned medium was centrifuged at 300 g for 5 minutes, and the supernatant was taken and passed through a 0.22 μm filter membrane to obtain the stem cell conditioned medium for future use.

[0070] 3D printed dressing

[0071] 2.3D modeling

[0072] Use the built-in laser of the 3D printer to scan the surface of the patient's scar to obtain data such as the size, shape, thickness of the wound, and the thickness of the epidermis and dermis of the patient's normal skin. Edit to make it produce a continuous surface image; use Studio4.0 softwa...

Embodiment 2

[0096] 1. Collection of stem cell conditioned medium

[0097] Take P3 generation umbilical cord mesenchymal stem cells and culture them with DMEM / F12+10% FBS. When the cells are in the exponential growth phase and the confluence reaches 80%, remove the culture supernatant, wash twice with PBS, and add 0.10ml / cm 2 After the phenol red-free 1640 was cultured for 72 hours, the conditioned medium was collected, and the collected conditioned medium was centrifuged at 200 g for 5 minutes, and the supernatant was taken, passed through a 0.22 μm filter membrane, and the resulting medium was the stem cell conditioned medium for use.

[0098] 3D printed dressing

[0099] 2. 3D modeling

[0100] Use the built-in laser of the 3D printer to scan the surface of the patient's scar to obtain data such as the size, shape, thickness of the wound, and the thickness of the epidermis and dermis of the patient's normal skin. Edit to make it produce a continuous surface image; use Studio4.0 softwa...

Embodiment 3

[0109] 1. Collection of stem cell conditioned medium

[0110] Take the umbilical cord mesenchymal stem cells of the P3 generation and culture them with DMEM / F12+10% FBS. When the cells are in the exponential growth phase and the confluence reaches 80%, remove the culture supernatant, wash twice with PBS, and add 0.2ml / cm 2 After the phenol red-free 1640 was cultured for 72 hours, the conditioned medium was collected, and the collected conditioned medium was centrifuged at 400 g for 5 minutes, and the supernatant was taken, passed through a 0.22 μm filter membrane, and the resulting medium was the stem cell conditioned medium for use.

[0111] 3D printed dressing

[0112] 2. 3D modeling

[0113] Use the built-in laser of the 3D printer to scan the surface of the patient's scar to obtain data such as the size, shape, thickness of the wound, and the thickness of the epidermis and dermis of the patient's normal skin. Edit to make it produce a continuous surface image; use Studio...

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