A single-cell protein detection system and its application

Abstract

The invention relates to a single cell protein detection system, which comprises a micro-fluidic chip module, a fluorescence excitation and detection module and a pressure control module, wherein the micro-fluidic chip module comprises a transparent substrate and a compression passage formed on the substrate; the compression passage is configured to a state that the cross section area is smaller than the cross section area of cells to be tested, so that the cells deform under the pressure effect and pass through the compression passage in an extruding way; the fluorescence excitation and detection module is used for performing fluorescence detection on the cells entering the compression passage; the pressure control module is used for providing the pressure so that the cells deform and pass through the compression passage. The invention also provides a method for performing detection by using the system. The method provided by the invention can be used for quantificationally detecting the single cell protein at high flux.

Description

technical field [0001] The invention relates to the field of biotechnology, and further relates to a single-cell protein detection system and a detection method using the detection system. Background technique [0002] Cancer is a disease caused by abnormal cell proliferation and apoptosis mechanism, which seriously threatens human life and health. In 2012 alone, there were about 14 million new cancer patients worldwide, and about 8.2 million people died of cancer. Tumor heterogeneity is one of the reasons why cancer treatment cannot be overcome. There are differences between tumor cells even in the same individual. Therefore, single-cell analysis plays an important role in the study of tumor heterogeneity. [0003] Protein is an important substance that constitutes an organism. It has catalytic functions, structural functions, transport functions, storage functions, motor functions, defense functions, regulatory functions, information transmission functions, genetic regulat...

AI Technical Summary

Problems solved by technology

[0006] (1) ELISA can only detect the protein status of population cells, and cannot detect the protein of single cells;

[0007] (2) The calibration microspheres used in flow cytometry can only quantify cell membrane proteins, while the quantitative modification method of fluorescent molecules inside the calibration microspheres is not yet mature, and it is impossible to use the traditional calibration microsphere method to quantify a single intracellular skeleton protein. Quantitative detection of expression;

[0008] (3) Some single-cell protein detection methods based on microfluidic technology also have disadvantages, such as micro-flow cytometry, microcavity microwell sequence, etc. The former lacks calibration methods and cannot quantitatively detect proteins; the latter has low throughput , the operation is complex

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