Composition and application thereof to preparation of product for improving chloasma

A composition and technology for chloasma, applied in the field of medicine, can solve the problems of limited and unsatisfactory improvement of chloasma, achieve inhibition of tyrosinase activity, improve chloasma, and reduce cell melanin content Effect

Inactive Publication Date: 2017-02-15
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
View PDF3 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, the above-mentioned treatments have very limite

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0050] Example 1 Preparation of umbilical cord mesenchymal stem cells

[0051] After washing the umbilical cord with PBS solution containing 200U / m double antibody for 3 times, cut into 2cm long sections, peel off the arterial and venous tubes and adventitia, and cut into 1-2mm 2 Small pieces, evenly attached to a 10cm petri dish, placed upside down in a 5% CO2 incubator at 37°C for 1-2h; the medium is X-VIVO15 serum-free medium;

[0052] Add X-VIVO15 serum-free medium containing 10ng / mL EGF, incubate at 37°C with 5% CO2 for 2 to 3 days and then change half of the medium. Continue to culture for 6 to 8 days. When the cells crawl out of the tissue block, discard the tissue block Continue training

[0053] When it grows to 80% confluence, pour the culture solution, wash it with PBS, add 0.25% trypsin for 1-2min, add FBS to stop the digestion, centrifuge at 400g for 5min to collect umbilical cord mesenchymal cells.

[0054] Resuspend umbilical cord mesenchymal cells in X-VIVO15 serum-fre...

Example Embodiment

[0056] Example 2 Preparation of stem cell extract A

[0057] The P2 and P3 generation stem cell culture supernatants prepared in Example 1 were collected and concentrated using a Slice200 tangential flow filter. The circulating filtration time was 3 hours. During this process, the large particles were removed by filtration through a 0.45 μm filter membrane. Filter through a 0.22μm filter membrane to remove small particles below 3kDa; the concentrated solution is made into a freeze-dried powder with a freeze dryer, which is the stem cell extract A.

Example Embodiment

[0058] Example 3 Preparation of Stem Cell Extract B

[0059] Collect the P2 or P3 generation cells prepared in Example 1, and add pure water to resuspend them to a cell density of 1×106 cell / mL~5×10 6 cell / mL, put in liquid nitrogen and quick-freeze for 5 minutes, and then defrost at 37℃;

[0060] After thawing, put in liquid nitrogen for quick freezing, repeat 3 times;

[0061] Ultrasonic oscillation for 3 minutes (power 300W, frequency 20Hz~40Hz) in an ice water bath at 0~4℃, 3 times of ultrasonic oscillation;

[0062] The resulting suspensions were combined and filtered through a 0.22 μm filter membrane under aseptic conditions to prepare stem cell extract B.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Inoculation densityaaaaaaaaaa
Login to view more

Abstract

The invention relates to the field of medicine, in particular to a composition and application thereof to the preparation of a product for improving chloasma. The composition provided by the invention comprises a Chinese angelica extract, a stem cell extract A and a stem cell extract B. Experiments show that the composition provided by the invention can reduce the relative activity of tyrosinase to be 89.8% and reduce the relative content of melanin in cells to be 82.8%, so that the composition provided by the invention can effectively inhibit the activity of the tyrosinase and reduce the melanin content of the cells. The effects are obviously more excellent than those of a control group which gives the Chinese angelica extract singly or gives the stem cell extract singly. In addition, the composition provided by the invention is used for improving the chloasma, the effective rate can reach 95% and the remission rate can reach 70%.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a composition and its application in preparing products for improving chloasma. Background technique [0002] Melasma, also known as liver spots, is a yellow-brown pigmentation on the face. Multiple symmetrical butterflies are distributed on the cheeks. It is more common in women, and the main reason is high estrogen level in the blood. Its onset is related to pregnancy, long-term oral contraceptives, and menstrual disorders. Chloasma is generally yellowish brown or dark brown patches, which are often distributed symmetrically on the cheeks and cheeks, and can also involve the periorbital, forehead, upper lip and nose, and the edges are generally more obvious. No subjective symptoms and general discomfort. The depth of stains is related to seasons, sun exposure, and endocrine factors. Stress, staying up late, and fatigue can aggravate skin lesions. [0003] Therapies for melasma are ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K36/232A61P17/00A61K35/28
CPCA61K35/28A61K36/232A61K2300/00
Inventor 葛啸虎陈海佳王一飞应杰张维敏
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap