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Composition and application thereof to preparation of product for improving chloasma

A composition and technology for chloasma, applied in the field of medicine, can solve the problems of limited and unsatisfactory improvement of chloasma, achieve inhibition of tyrosinase activity, improve chloasma, and reduce cell melanin content Effect

Inactive Publication Date: 2017-02-15
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, the above-mentioned treatments have very limited improvement effects on chloasma, which is hardly satisfactory.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 Preparation of umbilical cord mesenchymal stem cells

[0051] After rinsing the umbilical cord with PBS solution containing 200U / m double antibody for 3 times, cut it into 2cm long pieces, peel off the arterial and venous tubes and adventitia, and cut them into pieces of 1-2mm 2Small pieces, evenly attached to a 10cm culture dish, placed upside down in a 5% CO2 incubator at 37°C for 1-2 hours; the medium is X-VIVO15 serum-free medium;

[0052] Add X-VIVO15 serum-free medium containing 10 ng / mL EGF, culture at 37°C with 5% CO2 for 2 to 3 days, then change the medium in half, continue to culture for 6 to 8 days, and discard the tissue after the cells crawl out of the tissue continue to train;

[0053] After growing to 80% confluence, discard the culture medium, wash once with PBS, add 0.25% trypsin to digest for 1-2 minutes, add FBS to stop digestion, and collect umbilical cord mesenchymal cells by centrifugation at 400g for 5 minutes.

[0054] Resuspend umbil...

Embodiment 2

[0056] The preparation of embodiment 2 stem cell extract A

[0057] The P2 and P3 generation stem cell culture supernatants prepared in Example 1 were collected, concentrated using a Slice200 tangential flow filter, and the cycle filtration time was 3 hours. During this process, large particles were removed by filtration through a 0.45 μm filter membrane, and then Filter through a 0.22 μm filter membrane to remove small particles below 3 kDa; the concentrated solution is made into a freeze-dried powder by a freeze dryer, which is the stem cell extract A.

Embodiment 3

[0058] The preparation of embodiment 3 stem cell extract B

[0059] Collect the P2 or P3 generation cells prepared in Example 1, add pure water and resuspend until the cell density is 1×106cell / mL~5×10 6 cell / mL, quick-frozen in liquid nitrogen for 5 minutes, and thawed at 37°C after taking out;

[0060] After thawing, put it into liquid nitrogen for quick freezing, repeat 3 times;

[0061] Ultrasonic oscillation for 3 minutes in an ice-water bath at 0-4°C (power 300W, frequency 20Hz-40Hz), ultrasonic oscillation 3 times;

[0062] The resulting suspensions were combined and filtered through a 0.22 μm filter membrane under sterile conditions to obtain stem cell extract B.

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Abstract

The invention relates to the field of medicine, in particular to a composition and application thereof to the preparation of a product for improving chloasma. The composition provided by the invention comprises a Chinese angelica extract, a stem cell extract A and a stem cell extract B. Experiments show that the composition provided by the invention can reduce the relative activity of tyrosinase to be 89.8% and reduce the relative content of melanin in cells to be 82.8%, so that the composition provided by the invention can effectively inhibit the activity of the tyrosinase and reduce the melanin content of the cells. The effects are obviously more excellent than those of a control group which gives the Chinese angelica extract singly or gives the stem cell extract singly. In addition, the composition provided by the invention is used for improving the chloasma, the effective rate can reach 95% and the remission rate can reach 70%.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a composition and its application in preparing products for improving chloasma. Background technique [0002] Melasma, also known as liver spots, is a yellow-brown pigmentation on the face. Multiple symmetrical butterflies are distributed on the cheeks. It is more common in women, and the main reason is high estrogen level in the blood. Its onset is related to pregnancy, long-term oral contraceptives, and menstrual disorders. Chloasma is generally yellowish brown or dark brown patches, which are often distributed symmetrically on the cheeks and cheeks, and can also involve the periorbital, forehead, upper lip and nose, and the edges are generally more obvious. No subjective symptoms and general discomfort. The depth of stains is related to seasons, sun exposure, and endocrine factors. Stress, staying up late, and fatigue can aggravate skin lesions. [0003] Therapies for melasma are ...

Claims

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Application Information

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IPC IPC(8): A61K36/232A61P17/00A61K35/28
CPCA61K35/28A61K36/232A61K2300/00
Inventor 葛啸虎陈海佳王一飞应杰张维敏
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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