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A method for realizing microsphere single array injection
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A microsphere and sample injection technology, applied in laboratory containers, chemical instruments and methods, laboratory utensils, etc., can solve the problems of difficult to balance the focusing force, stagnant sample liquid, and too small driving force. Achieve the effect of good technical support, increased flexibility and diversity, and good optical performance
Active Publication Date: 2021-06-18
SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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[0007] However, the current research is to control the focus of the liquid flow by controlling the angle between the sample flow and the sheath flow channel in the microfluidic chip; the process of processing the chip with materials such as PMMA limits the shape of the channel to a square, resulting in The focusing force is difficult to balance; it is mainly used for liquid flow focusing and bubble formation. For microspheres with a certain shape and gravity, due to the theory of momentum conservation, the driving force of the fluid to the outlet direction comes from the sample flow, and the driving force is too large. If the injection port is under too much pressure, the liquid will easily overflow from the injection port; if the driving force is too small, the forward momentum of the liquid flow will be insufficient, and the sample liquid will stagnate, making it difficult to form a stable and continuous single-arranged focused flow
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Embodiment 1
[0034] Different injection capillary positions realize microsphere single array injection method:
[0035] (1) Design a cross chip, and use laser etching to obtain a cross chip with an inner diameter of 330 μm and a size of 2 cm × 2 cm, such as figure 1 shown.
[0036] (2) Pull one end of the capillary suitable for the inner diameter of the cross-chip, the inner diameter of the tip is 85 μm, remove part of the coating, insert it into the channel at any end of the above-mentioned cross-chip, and use it after completing the position setting and coaxiality of the outlet end of the capillary under the microscope Glass glue fixes the capillary, and the insertion position of the tip end is: the cross entrance, the cross center, the cross exit, and the cross exit 100 μm, as shown in Figure 2, use the thin tube to connect the capillary to the microsphere suspension delivery pump;
[0037] (3) Remove part of the coating from the three capillaries that are compatible with the inner dia...
Embodiment 2
[0047] Fluorescent imprinted microspheres with different concentrations of chlorpyrifos were tested by this method.
[0048] (1) Utilize the chip with the sharp capillary in the above method located at the intersection center of the cross to build a test system;
[0049] (2) Suspend 4mg of chlorpyrifos fluorescent imprinted microspheres in 10ml of suspension solution, and dilute the suspension with sheath liquid. After dilution, the concentration of microspheres is 0.05mg / ml, 0.1mg / ml, 0.2mg / ml, 0.4mg / ml, were injected into the chip for separation and detection, and obtained the relationship between the fluorescence value of the microsphere and the concentration, such as Figure 4 shown.
[0050] It can be seen from the figure that the microspheres can form a single arrangement in the chip of this design, and generate fluorescence under the excitation of the laser confocal system, and the number and concentration of the collected fluorescent microspheres show a good linear r...
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Abstract
The invention belongs to the technical field of microfluidic chips, and in particular relates to a method for realizing microsphere single-arrangement sample injection. Insert the thin tube into the cross microchip channel, insert a thin tube with a pointed end into any channel, deliver the suspension of microspheres to the pointed thin tube, and deliver it to the two thin tubes perpendicular to the pointed capillary Sheath liquid, the microsphere suspension to be transported is passed through the pointed capillary to the opposite thin tube to form a single-arrangement focused flow of microspheres, thereby realizing the injection of single-arrangement microspheres. The microfluidic device of the present invention can solve the problem of unstable focus signals caused by uneven forces in all directions during the single-arrangement focusing process of microspheres, and provides a miniaturized sample introduction method for microsphere detection.
Description
technical field [0001] The invention belongs to the technical field of microfluidic chips, and in particular relates to a method for realizing microsphere single-arrangement sample injection. Background technique [0002] The organic pollution rapid detection system is based on the identification and analysis of organic pollutant imprinted microspheres. According to the requirements of the particle size of the microspheres and the stability of the detection signal, it is first required to build a sampling device suitable for 10-15 μm microsphere sample liquid. At the same time, it solves the three key problems of fast and stable sample injection, single arrangement of microspheres, and stable optical performance of the detection area. [0003] Microfluidic technology is a popular interdisciplinary subject that emerged in the 1990s, and has the characteristics of rapidity, stability, and good controllability. In 2001, my country launched the 15 major projects "Basic Research...
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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/01G01N21/64B01L3/00
CPCB01L3/502707G01N21/01G01N21/6402G01N21/643
Inventor 张红王颜红周强
Owner SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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