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Application of Reyanning combined with antibiotics in interfering antibacterial effect against pathogenic bacteria

A technology against pathogenic bacteria and antibiotics, which can be used in antibacterial drugs, medical preparations containing active ingredients, plant raw materials, etc., and can solve problems such as drug resistance and poor effect.

Active Publication Date: 2021-12-10
TSING HUA DE REN XIAN HAPPINESS PHARMA
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The object of the present invention is to provide an application of Reyanning combined with antibiotics in intervening the antibacterial effect of anti-pathogenic bacteria, which overcomes the problems in the prior art that traditional Chinese medicine preparations and antibiotics are used alone to produce drug resistance and poor effect. Reyanning Combined with the antibiotics cefoxitin and linezolid, it has a synergistic effect, providing guidance for the clinical use of traditional Chinese medicine preparations and antibiotics in the treatment of methicillin-resistant Staphylococcus aureus infection

Method used

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  • Application of Reyanning combined with antibiotics in interfering antibacterial effect against pathogenic bacteria
  • Application of Reyanning combined with antibiotics in interfering antibacterial effect against pathogenic bacteria
  • Application of Reyanning combined with antibiotics in interfering antibacterial effect against pathogenic bacteria

Examples

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experiment example 1

[0022] 1 material

[0023] 1.1 Drug target proteins and MRSA-related genes

[0024] Find the chemical constituents of dandelion, Polygonum cuspidatum, Beibaijiang, and Scutellaria barbatae in RYN from "Dictionary of Traditional Chinese Medicine (Second Edition)"; , Metabolism, Excretion) properties to determine the druggable active ingredients; retrieve the human target proteins corresponding to these active ingredients from the PubChem database. 103, 256, 45, and 97 target proteins of Dandelion, Polygonum cuspidatum, Beibaijiang, and Scutellaria barbata were found. Retrieve the target proteins of Van and FOX in ChEMBL, ETCM and PubChem databases, and exclude non-empirical acquisitions and duplicates, and select 134 Van target proteins and 65 FOX target proteins; search for MRSA pathogenicity in Ensembl Bacteria, Uniprot and PubMed databases A total of 80 genes were selected by removing duplicates.

[0025] 1.2 Bacterial experimental materials

[0026] Reyanning (ReYanNing...

experiment example 2

[0055] Objective: To explore the synergistic effect of combined action of RYN and LNZ on MRSA.

[0056] METHODS: The minimum inhibitory concentrations (MICs) of RYN and LNZ against free MRSA strains were determined by broth microdilution method. The antibacterial effect of RYN and LNZ was studied by chessboard method. Detection of MRSA in biofilms by 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazole-5-carboxanilide (XTT) reduction method. The anti-biofilm effects of the two drugs were observed in the development and formation of biofilms. Viable cells stained with Syto9 in biofilms after drug administration were visualized using confocal scanning microscopy.

[0057] Results: The MRSA strain was sensitive to RYN, the MIC value was 1 / 2, while the MIC value of LNZ was 4 μg / ml. RYN significantly enhanced the activity of the tested antibiotics by reducing their MICs. The antibacterial effect of LNZ was more significant than that of RYN at 0h, and the antibacterial effect ...

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Abstract

The invention relates to the application of Reyanning combined with antibiotics in intervening the antibacterial effect of anti-pathogenic bacteria, which overcomes the problems of drug resistance and poor effect caused by traditional Chinese medicine preparations and antibiotics used alone. The combination of azoles and amide plays a synergistic effect, providing guidance for the clinical use of traditional Chinese medicine preparations and antibiotics in the treatment of methicillin-resistant Staphylococcus aureus infection. The technical scheme adopted in the present invention is: an application of Reyanning combined with antibiotics in intervening the antibacterial effect against pathogenic bacteria, Reyanning uses Reyanning mixture, the antibiotic is cefoxitin or linezolid, and the pathogen is methoxyl-resistant Staphylococcus aureus. The dilution ratio of Reyanning is 1:8, and when the concentration of cefoxitin is 4mg / ml, it is the minimum inhibitory concentration of the two drugs; the combination of 1 / 8MIC Reyanning and 1 / 2MIC linezolid is more effective than each single drug Has a stronger antibacterial effect.

Description

Technical field: [0001] The invention relates to an application method of traditional Chinese medicine preparation combined with antibiotics, in particular to the application of Reyanning combined with antibiotics in intervening the antibacterial effect against pathogenic bacteria. Background technique: [0002] Methicillin-resistant Staphylococcus aureus (MRSA) is the main pathogen causing community-acquired and nosocomial infections. MRSA infection has high morbidity and mortality and has become a major public health problem. Vancomycin (Vancomycin, Van) is the drug of choice for the treatment of MRSA infection, but its efficacy is declining. Cefoxitin (FOX) used to be the first-line drug for the treatment of MRSA. Due to its long-term and large-scale use, its sensitivity to MRSA has decreased. Although it is no longer clinically used for the treatment of MRSA, it is safe and cheap It is still widely used clinically in the treatment of infectious diseases. Linezolid (LN...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/546A61K36/84A61K31/5377A61P31/04
CPCA61K36/288A61K36/704A61K36/84A61K36/539A61K31/546A61K31/5377A61P31/04A61K2300/00
Inventor 杜成强
Owner TSING HUA DE REN XIAN HAPPINESS PHARMA
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