A method for identifying mature rapeseed honey

A honey and rapeseed technology, applied in the field of honey identification, can solve the problems of disrupting the market order, thin honey, inferior nutritional value, etc., and achieve the effect of maintaining market order and controlling the quality of honey

Active Publication Date: 2022-06-17
BEE RES INST CHINESE ACAD OF AGRI SCI
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The immature honey has not been fully brewed, the brewing time is short, the water content in the honey is high, the high molecular sugar is not completely decomposed, the content of bee enzymes is low, the honey is thin, and its nutritional value is far inferior to that of mature honey.
Unscrupulous merchants are driven by profit to collect immature honey, and in order to make the honey meet the requirements of the standard, heat and dehydrate the honey at high temperature. However, the heating process destroys the active substances in honey, especially polyphenols, and produces the products of Maillard reaction, which seriously affects the nutritional value of honey.
[0004] At present, the identification of mature honey is cumbersome, and it is not suitable for users to identify
Among them, rapeseed honey is the largest and stable honey species, accounting for more than 40% of the total honey production throughout the year. However, the vast majority of rapeseed honey on the market is obtained by processing and concentrating immature honey, which seriously disrupts market order and damages consumer interest

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for identifying mature rapeseed honey
  • A method for identifying mature rapeseed honey
  • A method for identifying mature rapeseed honey

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The present embodiment provides a method for identifying mature rapeseed honey, comprising the following steps:

[0030] S1. Collect natural capped mature rapeseed honey from the bee farm as the sample to be tested

[0031] S2, the sample to be tested is pretreated to obtain honey extract

[0032] 1) Weigh 5 g of rape honey to be tested, put the honey into a 50 mL centrifuge tube, add 10 mL of pure water, and ultrasonically dissolve for 20 min to obtain an aqueous solution of honey;

[0033] 2) Install the SPE column on the solid phase extraction device, rinse with 5 mL of methanol and 5 mL of pure water respectively, and then add the honey solution, and the effective substances in the honey are adsorbed on the SPE column packing;

[0034] 3) The SPE column adsorbed with the effective substance of honey is first rinsed twice with 10 mL of pure water, and then eluted with 8 mL of methanol, and the eluent is collected;

[0035] 4) Concentrate the eluate to a solid with ...

Embodiment 2

[0050] Collect uncapped immature rape honey as the sample to be tested from the bee farm, adopt the method as described in implementation 1 to detect it, and the result is that the Q-TOF analysis total ion diagram of the immature rape honey extract is as follows: figure 1 As shown in A, the extraction chromatogram of immature rape honey extract Q-TOF analysis characteristic ion 285.0405 is as follows image 3 shown.

[0051] from figure 2 and image 3 The comparison shows that the retention time is 8.99±0.10min, and the quasi-molecular ion peak of 285.04±0.01 mass number can be used as the characteristic peak for identifying mature rapeseed honey. Only mature rapeseed honey contains this characteristic peak, while immature rapeseed honey does not contain this characteristic peak. of the characteristic peak. After repeated experiments many times, the result is still the same.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for identifying mature rapeseed honey, which uses liquid chromatography-quadrupole-time-of-flight mass spectrometry to detect the honey to be tested, with a retention time of 8.99±0.10min and a quasi-molecular ion peak of 285.04±0.01 mass number as the characteristic peak , when the detection spectrum contains the characteristic peaks, it is determined that the honey to be tested is mature rapeseed honey; liquid chromatography detection conditions: C18 chromatographic column; mobile phase A is 0.1% formic acid aqueous solution, and mobile phase B is methanol; 0-1min The volume fraction of mobile phase B is increased to 5%, the volume fraction of mobile phase B is increased from 5% to 55% in 1‑6min, the volume fraction of mobile phase B is increased from 55% to 95% in 6‑20min, and the volume fraction of mobile phase B is increased in 20‑26min Maintained at 95%, the volume fraction of mobile phase B decreased to 5% in 26‑27min. The method of the invention is fast and accurate in identifying mature rapeseed honey.

Description

technical field [0001] The invention belongs to the field of honey identification, in particular to a method for identifying mature rapeseed honey. Background technique [0002] Honey has been favored by consumers for a long time due to its rich active substances and high nutritional value, but it also suffers from the threat of honey adulteration. The typical honey adulteration behavior is syrup blending, in addition to the use of ion-exchange resin to decolorize honey to make it shoddy, confuse the type of honey source, artificially feed sucrose during the collection period, and harvest immature honey to increase yield and other fraudulent practices. [0003] The practice of collecting immature honey to increase honey production for higher yields has also become more common in recent years. Ripe honey refers to honey that has been fully brewed by bees. After the bees have brewed the honey, it is stored in the hive and sealed with beeswax. At this time, the honey cap mus...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & AuthorityPatents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/72
CPCG01N30/02G01N30/06G01N30/34G01N30/72G01N2030/062
Inventor王凯吴黎明彭文君赵柳微郭娜娜薛晓锋
OwnerBEE RES INST CHINESE ACAD OF AGRI SCI