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miRNAs affecting chicken growth rate and its application

A technology of growth rate and detection kit, which is applied in the field of miRNA that affects the growth rate of chickens, can solve the problems of muscle production, growth rate and insufficient feed utilization, and achieve the effect of simple and fast detection method

Active Publication Date: 2022-04-15
YANGZHOU UNIV
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  • Claims
  • Application Information

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Problems solved by technology

However, yellow feather broilers are deficient in muscle yield, growth rate and feed utilization efficiency, and there is still great potential for improvement

Method used

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  • miRNAs affecting chicken growth rate and its application
  • miRNAs affecting chicken growth rate and its application
  • miRNAs affecting chicken growth rate and its application

Examples

Experimental program
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Embodiment 1

[0024] Example 1 Based on the joint analysis of RNA-seq and mRNA, the miRNA that affects the growth rate of chickens --- Novel-gga-miR-145 is obtained

[0025] 1. Collection of sequencing samples

[0026] The sequencing samples came from Jinghai yellow chickens of Jiangsu Jinghai Poultry Industry Group Co., Ltd. with the same feeding and management level. At the age of 300 days, three healthy fast and slow long Jinghai yellow chicken hens with similar body weight were selected. After slaughter, the leg muscle tissue was stored in liquid nitrogen for RNA-seq. The sequencing was completed by Beijing Biomarker Biotechnology Co., Ltd. The specific steps included RNA extraction, RNA quality detection and library construction. See Table 1 for the determination of the relevant traits of the collected individuals.

[0027] Table 1. Sequencing indicators of fast and slow long Jinghai yellow chickens

[0028]

[0029] Note: Different lowercase letters on the same row of shoulders ...

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Abstract

The present invention discloses a miRNA that affects chicken growth rate, and the nucleotide sequence of the miRNA is shown in SEQ ID NO.1. The invention also discloses specific primers for detecting the expression level of the miRNA affecting chicken growth rate. The invention also discloses a detection kit, which includes the specific primer. The invention also discloses the application of the miRNA, the specific primer and the detection kit in verifying the growth speed of chickens. The invention discloses a detection method of the detection kit. Finally, the invention discloses a method for increasing or slowing down the growth speed of chickens. The detection kit provided by the invention can be used to detect the expression level of the miRNA in chicken muscle tissue, and the detection method is simple and quick. The present invention can also use the expression level of the miRNA to control the growth speed of the chicken, and increase or slow down the growth speed of the chicken according to actual needs in the actual breeding process.

Description

technical field [0001] The invention belongs to the field of bioengineering, and in particular relates to a miRNA affecting the growth rate of chickens and an application thereof. Background technique [0002] Yellow-feather broiler is derived from our country's native poultry species, and it has a unique flavor and better meat quality than white-feather broiler. In my country, the output of yellow-feathered broilers has increased year by year, and has been basically equal to that of white-feathered broilers in the past two years. However, yellow-feathered broilers have deficiencies in muscle yield, growth rate and feed utilization, and there is still great potential for improvement. [0003] miRNA is a non-coding RNA with a length of about 20nt, which performs post-transcriptional regulation by binding to the 3′UTR region of the target gene. A large number of studies have shown that miRNA plays an important role in the process of cell proliferation, differentiation, apopt...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12Q1/6851C12N15/113C12N15/11
CPCC12Q1/6888C12Q1/6851C12Q2600/158C12Q2600/178C12Q2600/166C12Q2531/113C12Q2545/101C12Q2563/107
Inventor 张跟喜周楷智吴鹏飞何明亮张欣超张涛谢恺舟戴国俊王金玉
Owner YANGZHOU UNIV
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