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MiRNA influencing chicken growth rate and application thereof

A growth rate and detection kit technology is applied in the field of miRNA that affects the growth rate of chickens.

Active Publication Date: 2021-04-09
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, yellow feather broilers are deficient in muscle yield, growth rate and feed utilization efficiency, and there is still great potential for improvement

Method used

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  • MiRNA influencing chicken growth rate and application thereof
  • MiRNA influencing chicken growth rate and application thereof
  • MiRNA influencing chicken growth rate and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Based on the joint analysis of RNA-seq and mRNA, the miRNA affecting chicken growth rate --- Novel-gga-miR-133 was obtained

[0025] 1. Collection of sequencing samples

[0026] The sequencing samples came from Jinghai yellow chickens of Jiangsu Jinghai Poultry Industry Group Co., Ltd. with the same feeding and management level. At the age of 300 days, three healthy fast and slow long Jinghai yellow chicken hens with similar body weight were selected. After slaughter, the leg muscle tissue was stored in liquid nitrogen for RNA-seq. The sequencing was completed by Beijing Biomarker Biotechnology Co., Ltd. The specific steps included RNA extraction, RNA quality detection and library construction. See Table 1 for the determination of the relevant traits of the collected individuals.

[0027] Table 1. Sequencing indicators of fast and slow long Jinghai yellow chickens

[0028]

[0029] Note: Different lowercase letters on the same row of shoulders indicate s...

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PUM

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Abstract

The invention discloses miRNA influencing the chicken growth rate. A nucleotide sequence of the miRNA is as shown in SEQ ID NO.1. The invention also discloses a specific primer for detecting the expression quantity of the miRNA influencing the chicken growth rate. The invention also discloses a detection kit. The kit comprises the specific primer. The invention also discloses application of the miRNA, the specific primer and the detection kit in verifying the chicken growth rate. The invention discloses a detection method of the detection kit. The invention finally discloses a method for increasing or reducing the chicken growth rate. The detection kit provided by the invention can be used for detecting the expression quantity of the miRNA in chicken muscle tissues, and the detection method is simple and rapid. According to the invention, the chicken growth rate can be controlled by utilizing the expression quantity of the miRNA, and the chicken growth rate can be increased or decreased according to actual needs in the actual breeding process.

Description

technical field [0001] The invention belongs to the field of bioengineering, and in particular relates to a miRNA affecting the growth rate of chickens and an application thereof. Background technique [0002] Yellow-feather broiler is derived from our country's native poultry species, and it has a unique flavor and better meat quality than white-feather broiler. In my country, the output of yellow-feathered broilers has increased year by year, and has been basically equal to that of white-feathered broilers in the past two years. However, yellow-feathered broilers have deficiencies in muscle yield, growth rate and feed utilization, and there is still great potential for improvement. [0003] miRNA is a non-coding RNA with a length of about 20nt, which performs post-transcriptional regulation by binding to the 3′UTR region of the target gene. A large number of studies have shown that miRNA plays an important role in the process of cell proliferation, differentiation, apopt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6851C12N15/113C12N15/11
CPCC12Q1/6888C12Q1/6851C12Q2600/158C12Q2600/178C12Q2600/166C12Q2531/113C12Q2545/101C12Q2563/107
Inventor 张跟喜周楷智吴鹏飞何明亮张欣超张涛谢恺舟戴国俊王金玉
Owner YANGZHOU UNIV
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