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Total coliform determination kit and use method thereof

A technology of coliforms and kits, which is applied in the field of total coliforms determination kits, can solve the problems of high risk of microbial contamination, cumbersome operation, and low work efficiency, and achieve the effects of eliminating disinfection, convenient operation, and simple structure

Pending Publication Date: 2021-04-16
湖南鸿润生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional detection method is to take 5 glass test tubes with the above-mentioned prepared culture medium on the test tube rack, remove the rubber stopper, take 10mL water sample in the water sample collection bottle with a sample gun and shake it well in the test tube, a total of 5 tubes, Cover the rubber stopper and culture and detect coliform bacteria in the incubator. This method requires the preparation of culture medium and disinfection of test items. The operation is cumbersome and the work efficiency is low. The more times of filling, the greater the risk of microbial contamination.
[0003] The total coliform determinatio

Method used

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  • Total coliform determination kit and use method thereof
  • Total coliform determination kit and use method thereof
  • Total coliform determination kit and use method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Embodiment one, such as figure 1 As shown, a total coliform assay kit of the present invention includes a box body 1, and four partitions 2 are arranged in the box body, and the partition boards divide the inside of the box body into five columns of independent cells 3. The height of the partition is lower than the height of the box body.

[0026] The top surface of the box body of this embodiment is provided with a cover body used for sealing the box body. The cover body is a sealing film 4, and a dividing line is provided on the sealing film. The opening of the box body top surface can be formed along the dividing line. Examples also include a cover 5 that fits on the sealing film.

[0027] Embodiment 2 of the present invention refers to figure 2 , comprising a box body, the box body is provided with nine partitions, the partition boards divide the inside of the box body into ten rows of independent cells, and the height of the partition boards is lower than the he...

Embodiment 3

[0029] Embodiment three, embodiment four see respectively image 3 , Figure 4 , The difference from Embodiment 1 is that the partition plate is at the same height as the box body, and a notch 21 or a through hole 22 is provided on one side of the partition plate.

Embodiment 5

[0031] Use embodiment one adopts 5 tubes of lactose bile salt fermentation method to carry out the mensuration of total coliform group, the using method of a kind of total coliform group assay kit of the present embodiment, comprises the steps:

[0032] Step 1, after sterilizing the total coliform group determination kit, seal the top surface, and sterilize for later use;

[0033] Step 2, preparation of medium, double-material concentrated lactose-peptone culture solution (50ml / tube), set aside;

[0034] Step 3, 100ml collection bottle to collect water samples, set aside;

[0035] Step 4, when measuring the coliform group, pour out the water sample in the water sample collection bottle until 50ml of water sample remains, take 1 tube of culture medium, which contains 50ml of culture medium, and pour it into the above water containing 50ml of water sample Put it in the sample collection bottle and shake it well to make a mixture of culture medium and water sample;

[0036] Pou...

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Abstract

The invention discloses a total coliform determination kit and a use method, the total coliform determination kit comprises a kit body, a plurality of clapboards are arranged in the kit body, the clapboards divide the interior of the kit body into a plurality of columns of independent cells, and the clapboards are flush with or lower than the height of the kit body. The total coliform determination kit has the advantages of being simple in structure, convenient to operate, and capable of effectively increasing the measurement rate in the coliform measurement process in a laboratory or a factory and reducing secondary pollution during culture medium split charging.

Description

technical field [0001] The invention relates to a kit for measuring total coliform bacteria and a use method in particular. Background technique [0002] When testing coliform bacteria in water samples in the laboratory, the traditional method is still the mainstream detection method for coliform bacteria because of its low cost and convenience. The traditional detection method is to take 5 glass test tubes with the above-mentioned prepared culture medium on the test tube rack, remove the rubber stopper, take 10mL water sample in the water sample collection bottle with a sample gun and shake it well in the test tube, a total of 5 tubes, Cover the rubber stopper and culture and detect coliform bacteria in the incubator. This method requires the preparation of culture medium and disinfection of test items. The operation is cumbersome and the work efficiency is low. The more times of filling, the greater the risk of microbial contamination. [0003] The total coliform determin...

Claims

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Application Information

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IPC IPC(8): C12Q1/10C12M1/34C12M1/00C12R1/19
Inventor 王佑雄
Owner 湖南鸿润生物科技有限公司