Rapid rewarming equipment for hematopoietic stem cells
A hematopoietic stem cell and stem cell technology, applied in the field of stem cell resuscitation, can solve the problems of difficult temperature measurement of stem cells, easy damage of stem cells, and easy fragmentation of stem cell storage bags, so as to ensure heat transfer efficiency, maintain cell stability, and improve rewarming. The effect of efficiency
Pending Publication Date: 2021-12-28
ANHUI PROVINCIAL HOSPITAL
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[0006] Aiming at the deficiencies of the prior art, the present invention provides a rapid rewarming device for hematopoietic stem cells, which has the advantages of high rewarming stability, good protection effect on stem cells,
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[0027] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.
[0028] As introduced in the background technology, there are deficiencies in the prior art. In order to solve the above technical problems, the present application proposes a rapid rewarming device for hematopoietic stem cells.
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The invention relates to the technical field of stem cell resuscitation, and discloses rapid rewarming equipment for hematopoietic stem cells. The rapid rewarming equipment comprises a water bath box body, a water bath is arranged in the water bath box body, and a heating mechanism, a water flow stirring mechanism and a shaking table are arranged in the water bath; a controller and a shaking driving mechanism are arranged in a water bath box body shell; and a temperature detection mechanism is arranged in the shaking table. The rewarming temperature of the stem cells is divided into three temperature intervals, when the temperature of the stem cells is within each temperature interval, different rewarming strategies are adopted, and the controller controls the heating mechanism to adjust the water bath temperature, controls the water flow stirring mechanism to adjust the stirring speed of water flow and controls the shaking driving mechanism to adjust the shaking frequency of the shaking table, so that program rewarming is carried out under the control of the controller, the rewarming efficiency of the stem cells is improved while the cell stability of rewarming of the stem cells is effectively maintained, the stem cells can be quickly rewarmed within 1-2 minutes, and the activity of the stem cells after rewarming is guaranteed.
Description
technical field [0001] The invention relates to the technical field of stem cell resuscitation, in particular to a rapid rewarming device for hematopoietic stem cells. Background technique [0002] Hematopoietic stem cells are usually stored at -196°C. When hematopoietic stem cells need to be used, the stem cells need to be rewarmed so that they are close to the body temperature of the human body, so that the rewarmed stem cells can become active again. Among them, the common rewarming method of stem cells is to thaw and rewarm stem cells in a constant temperature water bath. [0003] In the common water bath rewarming process, due to the large gap between the temperature of the water bath and the initial temperature of the stem cells, the temperature of the stem cells rises rapidly in the early stage of recovery, and it is difficult to monitor the real-time temperature of the stem cells, making it difficult to judge the degree of recovery of the stem cells. It is difficult...
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Login to view more IPC IPC(8): C12M1/38C12M1/36C12M1/34C12M1/12C12M1/02C12M1/00
CPCC12M23/14C12M27/06C12M27/16C12M29/04C12M41/12C12M41/22C12M41/36C12M41/42
Inventor 吴云贺雪梅王营营黄璐贾成珍
Owner ANHUI PROVINCIAL HOSPITAL
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