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Directional amplification method for bioremediation indigenous microorganisms in rivers and lakes and application of directional amplification method

A bioremediation and microbial technology, which is applied in the field of environmental restoration and sewage treatment, can solve the problems of microbial populations without directional amplification or colony enrichment screening, single-functional microorganisms, and poor effect sustainability, etc., and achieve high fixed microbial biomass , The effect lasts for a long time and the removal rate is fast

Pending Publication Date: 2022-02-15
海绵城市建设(上海)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] For exogenous microbial preparations environmental adaptability, biological safety, high price, single population, poor persistence of effect, etc., and the production process of indigenous microbial preparations is often aimed at single-functional microorganisms, without directional amplification and functional microbial populations or community enrichment screening , low application efficiency and other issues, the purpose of the present invention is to provide a method for directional amplification of indigenous microorganisms for bioremediation of rivers and lakes and its application

Method used

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  • Directional amplification method for bioremediation indigenous microorganisms in rivers and lakes and application of directional amplification method
  • Directional amplification method for bioremediation indigenous microorganisms in rivers and lakes and application of directional amplification method
  • Directional amplification method for bioremediation indigenous microorganisms in rivers and lakes and application of directional amplification method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0092] This embodiment provides a method for directional amplification of indigenous nitrifying bacteria flora.

[0093] (1) Enrichment culture

[0094] Get 10kg of river water, add 1kg of cleared river and lake bottom mud, add nitrifying bacteria flora enrichment medium, and cultivate.

[0095] Nitrifying bacteria flora enrichment medium:

[0096]

[0097] Add in 10kg of water: aerate until the dissolved oxygen remains above 4mg / l, add calcium carbonate to keep the pH at 7-8, cultivate for 3-7d, and when the ammonia nitrogen in the river water drops below 20mg / l, add 0.5mg / l flocculant to precipitate , to collect the precipitate.

[0098] (2) Expansion of training

[0099] Add the corresponding expansion medium to the 20kg culture tank with 10% of the original concentrated liquid of the indigenous nitrifying bacteria flora, add the corresponding concentrated medium, and carry out large-scale cultivation of the functional indigenous microbial flora, and finally form a fu...

Embodiment 2

[0111] This embodiment provides a method for directional amplification of indigenous denitrifying bacterial flora.

[0112] (1) Enrichment culture

[0113] Get 10kg of river water, add 1kg of cleaned river and lake sediment, add denitrifying bacteria flora enrichment medium, and cultivate.

[0114] Denitrifying bacteria flora enrichment medium: add in 10kg water:

[0115]

[0116]

[0117] Stir, cultivate for about 15 days, until the pH drops to 3-4, add 0.5 mg / l flocculant, precipitate, and collect the precipitate.

[0118] Put all the sediment into the denitrifying bacteria flora enrichment medium for secondary culture without concentration to obtain the original species of denitrifying bacteria flora.

[0119] (2) Expansion of training

[0120] Add the corresponding expansion medium to the 20kg culture tank at 20%, add the corresponding concentrated medium, and carry out large-scale cultivation of the functional indigenous microbial flora, and finally form a functi...

Embodiment 3

[0130] This example provides a method for directional amplification of indigenous aerobic heterotrophic bacterial flora.

[0131] (1) Enrichment culture

[0132] Take 10kg of river water, add 1kg of cleaned river and lake sediment, add aerobic heterotrophic bacterial flora enrichment medium, and carry out enrichment culture.

[0133] Aerobic heterotrophic bacterial flora enrichment medium: add in 10kg water:

[0134]

[0135] Aerate until the dissolved oxygen remains above 4 mg / l, cultivate for about 15 days, adjust the pH to 7-7.3, add 0.5 mg / l flocculant, settle, and collect the sediment.

[0136] All the sediments are put into the enrichment medium of aerobic heterotrophic bacterial flora for secondary culture without concentration to obtain the original species of aerobic heterotrophic bacterial flora.

[0137] (2) Expansion of training

[0138] Add 20% of the original species of aerobic heterotrophic bacterial flora to the corresponding expansion medium and add it t...

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Abstract

The invention relates to the technical field of environmental remediation and sewage treatment, in particular to a directional amplification method for bioremediation of indigenous microorganisms in rivers and lakes and application of the directional amplification method. According to the invention, three indigenous microorganism functional florae, i.e., nitrifying bacteria florae, aerobic heterotrophic florae and denitrifying bacteria florae, are screened through directional amplification and enrichment, and are applied to water quality purification and ecological restoration of rivers and lakes in combination with technologies such as river aeration and bottom mud bioremediation. The indigenous microbial preparation is taken from river sediment, is high in adaptability, can be directly planted in functional microbial populations or communities of ecological systems of rivers and lakes, realizes high-efficiency water purification and sediment remediation, and is good in effect persistence; no biological safety problem exists, and the problems of biological invasion, ecological risks and the like do not exist; the method is low in production price, can be widely applied to black and odorous riverway treatment and urban riverway water quality improvement, and can be matched with riverway aeration, bottom mud bioremediation and other technologies according to specific conditions to achieve one-river-one-strategy and one-river-one-bacterium effects and improve the riverway treatment efficiency.

Description

technical field [0001] The present invention relates to the technical field of environmental remediation and sewage treatment, in particular to a directional amplification method and application of indigenous microorganisms used for bioremediation of rivers and lakes. Background technique [0002] At present, there are mainly physical methods, chemical methods and biological methods for river water quality maintenance and restoration. Physical methods include river aeration, salvage, adding adsorbents, sediment shielding, water exchange and dilution, etc. The investment is large, the operation cost is high, and it is difficult to last. ; The chemical method is mainly to put oxidants, flocculants, curing agents and other chemical agents into rivers and lakes, which has a large risk of secondary pollution; bioremediation is to put microbial agents or microbial growth promoters, enzyme preparations, etc. into the water body or sediment to strengthen Microbes degrade pollutants,...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34
CPCC12N1/20C02F3/341Y02A20/402
Inventor 王炜
Owner 海绵城市建设(上海)有限公司
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