Glycosylated hemoglobin detection kit and detection method thereof

Abstract

The invention discloses a glycosylated hemoglobin detection kit and a detection method thereof. The kit comprises a solution A and a solution B, the liquid A is prepared from sinapic acid, acetonitrile, trifluoroacetic acid and water; the solution B is prepared from trifluoroacetic acid and water. The glycosylated hemoglobin detection kit provided by the invention has the advantages of cheap and easily available raw materials, simple process and suitability for large-scale production and application, and when the glycosylated hemoglobin detection kit provided by the invention is used for detecting glycosylated hemoglobin, not only can glycosylated hemoglobin variants be detected, but also the glycosylated hemoglobin variants can be detected. In addition, detection errors caused by different glycosylation groups and special differences of glycosylated hemoglobin variants can be effectively avoided, and the accuracy and precision of results are guaranteed.

Description

technical field [0001] The invention relates to the technical field of glycosylated hemoglobin detection. More specifically, it relates to a glycated hemoglobin detection kit and a detection method thereof. Background technique [0002] Diabetes is an endocrine and metabolic disease, and its incidence is second only to cardiovascular diseases and tumors. In recent years, the incidence of diabetes has been on the rise. It is a worldwide public health problem that seriously threatens human health. The traditional diagnosis of diabetes is mainly fasting blood glucose, postprandial blood glucose or oral glucose tolerance test and other methods, but the blood glucose parameters measured by the above methods only represent the instantaneous blood glucose level when blood is drawn, and the measurement results are not accurate. [0003] Glycated hemoglobin (HbA1c) is a stable compound in which glucose in blood is covalently bound to the N-terminal valine residue of the beta chain o...

AI Technical Summary

Problems solved by technology

Among them, capillary electrophoresis can theoretically detect common variants, and can prompt the existence of variants, but the whole experiment takes a long time, there are many external interference factors, and the operator has high technical requirements

Whether the ion-exchange high-performance liquid chromatography can indicate the existence of the variant is

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