A kind of plant essential oil composition for antibacterial and preparation method thereof

Abstract

The present application discloses a plant essential oil composition for antibacterial use and a preparation method thereof, and the technical field of antibacterial compositions. The plant essential oil composition comprises borneol essential oil, silver gray chrysanthemum essential oil, lilac essential oil, a hydrophilic emulsifier, a water-soluble cationic antibacterial agent and deionized water. The plant essential oil composition of the present invention has the characteristics of being natural, safe, efficient, non-toxic and having a broad spectrum of antibacterial properties.

Description

Technical Field

[0001] The invention relates to the technical field of antibacterial compositions, and in particular to an antibacterial plant essential oil composition and a preparation method thereof. Background Art

[0002] Studies have shown that bacteria can remain on surfaces that are frequently touched, such as door handles, faucets, TV remote controls and telephones. Bacteria can also spread by attaching to tiny dust particles or by spreading in the air through tiny water droplets when coughing, sneezing or talking. Currently, in order to block the spread of bacteria, chlorine-containing disinfectants, peroxide disinfectants, ethanol, etc. are mainly used for sterilization and disinfection. However, these chemicals have caused damage and pollution to the environment, and are very harmful to health when they come into contact with human skin or are inhaled into the human body.

[0003] Natural products have always been an important source in drug development. Compared with chemically synthesized molecules, natural products have higher structural complexity, more novel skeletons, and better drug-like properties. Plant essential oils are extracted from the flowers, leaves, roots, bark, fruits, seeds, resins, etc. of herbal plants by distillation and pressing.

[0004] The chemical molecules such as terpenes, aldehydes, lipids, alcohols in plant essential oils have unique composition and biological activity, so it has strong broad-spectrum antibacterial and bactericidal, antioxidant, anti-inflammatory and growth-promoting effects, and has the characteristics of antibacterial, natural and non-toxic. In recent years, many researchers have proved through experiments that plant essential oils have a certain inhibitory effect on the growth of fungi, and are one of the most promising antibacterial agents. The different components of plant essential oils can interact with each other to improve the antibacterial effect. Therefore, it is necessary to develop a plant essential oil composition so that its active ingredients can achieve a strong antibacterial effect. Summary of the invention

[0005] In view of the above problems, the present invention provides a natural, safe, efficient, non-toxic and broad-spectrum antibacterial plant essential oil composition.

[0006] The technical scheme adopted by the present invention is as follows: The present invention provides an antibacterial plant essential oil composition and a preparation method thereof, comprising borneol essential oil, silver-gray chrysanthemum essential oil, lilac essential oil, a hydrophilic emulsifier, a water-soluble cationic antibacterial agent and deionized water.

[0007] Borneol essential oil is a compound essential oil distilled and extracted from Camphor tree. The active ingredient, borneol, has bactericidal, regenerative and detoxifying effects.

[0008] Chrysanthemum argenteum essential oil is extracted from the flowers of Chrysanthemum argenteum. Chrysanthemum argenteum has gray-white soft hairs, silver-gray leaves and yellow flowers, elegant leaves, beautiful plant shape, and the whole plant is fragrant, so it is often used as an ornamental plant. Chrysanthemum argenteum contains a variety of volatile active ingredients, rich in sesquiterpenoid compounds, and has significant antibacterial, antitumor, antiviral and other pharmacological effects.

[0009] Lilac essential oil is an essential oil extracted from lilac flowers. It contains dozens of compounds such as phenols, terpenes, glycosides, etc., and has broad-spectrum antibacterial, anti-inflammatory, and antiviral effects.

[0010] The mixture of three plant essential oils, namely borneol essential oil, silver-gray chrysanthemum essential oil and lilac essential oil, not only has fragrance, but also, due to the different antibacterial mechanisms and antibacterial spectra of each essential oil component, the compound combination of essential oils produces a synergistic reaction between the components, further exerting a synergistic effect, which is beneficial to overcome the narrow spectrum of a single antibacterial component and enhance the overall antibacterial ability.

[0011] This application uses deionized water as a solvent, and the ingredients are safe; the use of water-soluble cationic antibacterial agents can further enhance the antibacterial ability and improve the broad-spectrum antibacterial effect; the hydrophilic emulsifier can better disperse the water-insoluble plant essential oils evenly in the water system.

[0012] Optionally, the raw material components account for the following proportions by mass: 0.1-2% borneol essential oil, 0.1-2% silver-gray chrysanthemum essential oil, 0.1-2% lilac essential oil, 0.1-1% hydrophilic emulsifier, 0.01-1% water-soluble cationic antibacterial agent, and the rest is deionized water. The antibacterial effect is excellent, and the mixed emulsion is delicate.

[0013] Preferably, the raw material components account for the following proportions by mass: 0.1-1% borneol essential oil, 0.1-1% silver-gray chrysanthemum essential oil, 0.1-1% lilac essential oil, 0.1-1% hydrophilic emulsifier, 0.01-1% water-soluble cationic antibacterial agent, and the rest is deionized water, which has the best antibacterial effect.

[0014] Preferably, the borneol essential oil, the silver-gray chrysanthemum essential oil, and the lilac essential oil are added in a mass ratio of 1:1:1, which is conducive to the synergistic reaction between the components of the three plant essential oils.

[0015] Optionally, the hydrophilic emulsifier is polyglycerol fatty acid ester. Polyglycerol fatty acid ester not only acts as an emulsifier and stabilizer, but also has a certain antibacterial effect.

[0016] Optionally, the water-soluble cationic antibacterial agent is hexamidine di(hydroxyethyl sulfonate). Hexamidine di(hydroxyethyl sulfonate) is a water-soluble cationic substance with broad-spectrum antibacterial and bactericidal properties, and has high bactericidal and antibacterial properties against various Gram-positive and Gram-negative bacteria, as well as various molds and yeasts.

[0017] The present invention also provides a method for preparing the above-mentioned plant essential oil composition, comprising the following steps:

[0018] Step 1: Heat and dissolve the emulsifier to form an emulsion;

[0019] Step 2: adding borneol essential oil, silver gray chrysanthemum essential oil, and lilac essential oil into the emulsion to form a first mixed solution;

[0020] Step 3: dissolving a water-soluble cationic antimicrobial agent in deionized water to form a second mixed solution;

[0021] Step 4: adding the second mixed solution into the first mixed solution, and stirring and mixing to obtain a crude emulsion.

[0022] Optionally, the first mixed liquid is an oil phase, and the second mixed liquid is an aqueous phase.

[0023] Optionally, in step 1, the heating temperature of the emulsifier is 70-90° C. Preferably, the heating temperature of the emulsifier is 80° C.

[0024] Optionally, in step 4, the stirring speed is 1200-1800 rpm. Preferably, the stirring speed is 1500 rpm.

[0025] The beneficial effects of the present invention are as follows: 1) The active ingredient of the present invention is plant essential oil, which has strong broad-spectrum antibacterial, anti-oxidant, anti-inflammatory and growth-promoting effects, and has the characteristics of high efficiency, antibacterial, natural, safe and non-toxic, and is suitable for all kinds of people. At the same time, the fresh formula can effectively remove the odor caused by wearing masks and other items for a long time, and keep fresh and comfortable.

[0026] 2) The antibacterial composition for the outer surface of an object of the present invention does not contain alcohol and other disinfecting chemical components, is harmless to inhale, can form a barrier film on the outer surface of the object, effectively blocks the entry of bacteria, improves the antibacterial protection effect of the object, and has high air permeability and long-term antibacterial effect. The antibacterial rate of Escherichia coli, Staphylococcus aureus, and Candida albicans is greater than 99.9%

[0027] 3) In the plant essential oil composition, the antibacterial mechanism and antibacterial spectrum of each essential oil component are different. Through the compound combination of essential oils, a synergistic reaction is generated between the components, and a synergistic effect is further exerted, which is conducive to overcoming the narrow spectrum of a single antibacterial component and enhancing the overall antibacterial ability. DETAILED DESCRIPTION

[0028] The present invention is described in detail and completely below through specific embodiments.

[0029] Example 1

[0030] The present embodiment provides a plant essential oil composition, which comprises, by mass percentage, 0.1% of borneol essential oil, 0.1% of silver gray chrysanthemum essential oil, 0.1% of lilac essential oil, 0.05% of hexamidine di(hydroxyethyl sulfonate), 0.5% of polyglycerol fatty acid ester, and the rest is deionized water.

[0031] The specific preparation steps are as follows:

[0032] 1) heating the emulsifier polyglycerol fatty acid ester to 80° C. and stirring to dissolve to form an emulsion;

[0033] 2) adding borneol essential oil, silver gray chrysanthemum essential oil and lilac essential oil into the emulsion as the oil phase;

[0034] 3) dissolving hexamidine di(hydroxyethylsulfonic acid) salt in deionized water as the aqueous phase;

[0035] 4) The aqueous phase was added to the oil phase and stirred with a high-speed stirrer at a speed of 1500 rpm to form a coarse emulsion.

[0036] Example 2

[0037] The present embodiment provides a plant essential oil composition, which comprises, by mass percentage, 0.5% of borneol essential oil, 0.5% of silver gray chrysanthemum essential oil, 0.5% of lilac essential oil, 0.05% of hexamidine di(hydroxyethyl sulfonate), 0.5% of polyglycerol fatty acid ester, and the rest is water.

[0038] The specific preparation steps are as follows:

[0039] 1) heating the emulsifier polyglycerol fatty acid ester to 80° C. and stirring to dissolve to form an emulsion;

[0040] 2) adding borneol essential oil, silver gray chrysanthemum essential oil and lilac essential oil into the emulsion as the oil phase;

[0041] 3) dissolving hexamidine di(hydroxyethylsulfonic acid) salt in deionized water as the aqueous phase;

[0042] 4) The aqueous phase was added to the oil phase and stirred with a high-speed stirrer at a speed of 1500 rpm to form a coarse emulsion.

[0043] Example 3

[0044] The present embodiment provides a plant essential oil composition, which comprises, by mass percentage, 1% borneol essential oil, 1% silver gray chrysanthemum essential oil, 1% lilac essential oil, 0.05% hexamidine disulfonate, 0.5% polyglycerol fatty acid ester, and the rest water.

[0045] The specific preparation steps are as follows:

[0046] 1) heating the emulsifier polyglycerol fatty acid ester to 80° C. and stirring to dissolve to form an emulsion;

[0047] 2) adding borneol essential oil, silver gray chrysanthemum essential oil and lilac essential oil into the emulsion as the oil phase;

[0048] 3) dissolving hexamidine di(hydroxyethylsulfonic acid) salt in deionized water as the aqueous phase;

[0049] 4) The aqueous phase was added to the oil phase and stirred with a high-speed stirrer at a speed of 1500 rpm to form a coarse emulsion.

[0050] Comparative Example 1

[0051] The present embodiment provides a plant essential oil composition, which comprises, by mass percentage, 1% of chrysanthemum essential oil, 1% of lilac essential oil, 0.05% of hexamidine disulfonate, 0.5% of polyglycerol fatty acid ester, and the remainder of water.

[0052] The specific preparation steps are as follows:

[0053] 1) heating the emulsifier polyglycerol fatty acid ester to 80° C. and stirring to dissolve to form an emulsion;

[0054] 2) adding silver-gray chrysanthemum essential oil and lilac essential oil into the emulsion as the oil phase;

[0055] 3) dissolving hexamidine di(hydroxyethylsulfonic acid) salt in deionized water as the aqueous phase;

[0056] 4) The aqueous phase was added to the oil phase and stirred with a high-speed stirrer at a speed of 1500 rpm to form a coarse emulsion.

[0057] Comparative Example 2

[0058] This embodiment provides a plant essential oil composition, which comprises, by mass percentage, 1% borneol essential oil, 1% lilac essential oil, 0.05% hexamidine disulfonate, 0.5% polyglycerol fatty acid ester, and the remainder water.

[0059] The specific preparation steps are as follows:

[0060] 1) heating the emulsifier polyglycerol fatty acid ester to 80° C. and stirring to dissolve to form an emulsion;

[0061] 2) adding borneol essential oil and lilac essential oil into the emulsion as the oil phase;

[0062] 3) dissolving hexamidine di(hydroxyethylsulfonic acid) salt in deionized water as the aqueous phase;

[0063] 4) The aqueous phase was added to the oil phase and stirred with a high-speed stirrer at a speed of 1500 rpm to form a coarse emulsion.

[0064] Comparative Example 3

[0065] The present embodiment provides a plant essential oil composition, which comprises, by mass percentage, 1% borneol essential oil, 1% chrysanthemum essential oil, 0.05% hexamidine disulfonate, 0.5% polyglycerol fatty acid ester, and the remainder water.

[0066] The specific preparation steps are as follows:

[0067] 1) heating the emulsifier polyglycerol fatty acid ester to 80° C. and stirring to dissolve to form an emulsion;

[0068] 2) adding borneol essential oil and silver gray chrysanthemum essential oil into the emulsion as the oil phase;

[0069] 3) dissolving hexamidine di(hydroxyethylsulfonic acid) salt in deionized water as the aqueous phase;

[0070] 4) The aqueous phase was added to the oil phase and stirred with a high-speed stirrer at a speed of 1500 rpm to form a coarse emulsion.

[0071] Comparative Example 4

[0072] This embodiment provides a plant essential oil composition, which comprises, by mass percentage, 1% of lilac essential oil, 0.05% of hexamidine disulfonate, 0.5% of polyglycerol fatty acid ester, and the remainder of water.

[0073] The specific preparation steps are as follows:

[0074] 1) heating the emulsifier polyglycerol fatty acid ester to 80° C. and stirring to dissolve to form an emulsion;

[0075] 2) adding lilac essential oil into the emulsion as the oil phase;

[0076] 3) dissolving hexamidine di(hydroxyethylsulfonic acid) salt in deionized water as the aqueous phase;

[0077] 4) The aqueous phase was added to the oil phase and stirred with a high-speed stirrer at a speed of 1500 rpm to form a coarse emulsion.

[0078] Comparative Example 5

[0079] This embodiment provides a plant essential oil composition, which comprises, by mass percentage, 1% borneol essential oil, 0.05% hexamidine disulfonate, 0.5% polyglycerol fatty acid ester, and the remainder water.

[0080] The specific preparation steps are as follows:

[0081] 1) heating the emulsifier polyglycerol fatty acid ester to 80° C. and stirring to dissolve to form an emulsion;

[0082] 2) adding borneol essential oil into the emulsion as the oil phase;

[0083] 3) dissolving hexamidine di(hydroxyethylsulfonic acid) salt in deionized water as the aqueous phase;

[0084] 4) The aqueous phase was added to the oil phase and stirred with a high-speed stirrer at a speed of 1500 rpm to form a coarse emulsion.

[0085] Table 1 Allocation ratio of each group in different implementations

[0086]

[0087] Table 2 Test results of antibacterial effect of three common bacteria

[0088]

[0089] According to the above examples 1-3 and comparative examples 1-5, it is shown that one or two plant essential oils alone cannot achieve a good antibacterial effect on the three common bacterial species. For example, it can be seen from the comparison between Example 3 and Comparative Example 1 that the antibacterial rate of Escherichia coli is significantly reduced when borneol essential oil is lacking; it can be seen from the comparison between Example 3 and Comparative Examples 2, 4 and 5 that the antibacterial rate of Candida albicans is significantly reduced when the essential oil of chrysanthemum flower is lacking. However, when the three essential oils are used in combination, they have a good antibacterial effect on the three common bacterial species.

[0090] It can be seen from Table 2 above that according to the dissolution antibacterial (inhibitory) performance test method 2.1.11.3.2 of the "Technical Specifications for Disinfection", the antibacterial rate test is carried out. The antibacterial rate of the antibacterial composition for the outer surface of an object prepared in Example 3 against Escherichia coli, Staphylococcus aureus and Candida albicans is greater than 99.9%, and the result meets the requirements of GB15979 "Hygiene Standards for Disposable Sanitary Products".

[0091] 5 g of the composition prepared by the preparation method of Examples 1 to 3 and Comparative Examples 1-2 were respectively placed in 10 ml centrifuge tubes, and placed in a 54°C constant temperature box for 48 hours and a -5°C refrigerator for 48 hours for stability tests to observe whether the emulsion is uniform and whether there are impurities. The test results showed that the liquid of the composition was uniform and free of impurities, indicating that the prepared composition has excellent stability in both high and low temperature environments.

[0092] Table 3 Stability test

[0093]

[0094] The above description is only a preferred embodiment of the present invention, and does not limit the patent protection scope of the present invention. Any equivalent structural transformation made using the contents of the present invention specification, directly or indirectly used in other related technical fields, is also included in the protection scope of the present invention.

Claims

1. A plant essential oil composition for antibacterial use, characterized in that: It includes borneol essential oil, silver gray chrysanthemum essential oil, lilac essential oil, hydrophilic emulsifier, water-soluble cationic antibacterial agent and deionized water; Among them, by mass percentage, the proportion of each raw material component is: borneol essential oil 0.5%, silver gray chrysanthemum essential oil 0.5%, lilac essential oil 0.5%, hydrophilic emulsifier 0.5%, water-soluble cationic antibacterial agent 0.05%, and the rest is deionized water; The borneol essential oil, silver-gray chrysanthemum essential oil, and lilac essential oil are added in a mass ratio of 1:1:1; The water-soluble cationic antibacterial agent is hexamidine di(hydroxyethylsulfonate) salt; The hydrophilic emulsifier is polyglycerol fatty acid ester.

2. The antibacterial plant essential oil composition according to claim 1, wherein The steps include: Step 1: heating and dissolving the polyglycerol fatty acid ester to form an emulsion; Step 2: adding borneol essential oil, silver-gray chrysanthemum essential oil, and lilac essential oil into the emulsion to form a first mixed solution; Step 3: dissolving hexamidine di(hydroxyethylsulfonic acid) salt in deionized water to form a second mixed solution; Step 4: adding the second mixed solution into the first mixed solution, and stirring and mixing to obtain a crude emulsion.

3. The antibacterial plant essential oil composition according to claim 2, characterized in that: The first mixed liquid is an oil phase, and the second mixed liquid is an aqueous phase.

4. The antibacterial plant essential oil composition according to claim 2, characterized in that: In step 1, the heating temperature of the polyglycerol fatty acid ester is 70-90°C.

5. The antibacterial plant essential oil composition according to claim 2, characterized in that: In step 4, the stirring speed is 1200-1800 rpm.

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