Method for promoting aloe polysaccharide to form pervaporation membrane

Abstract

The invention discloses a method for promoting aloe polysaccharide to form a pervaporation membrane. The method can form a pervaporation membrane on the surface of an aloe polysaccharide liquid. The pervaporation membrane has a significant difference in biological activity from the aloe polysaccharide liquid and can significantly promote the regeneration of hair follicles in wounds. The method uses a device for promoting aloe polysaccharide to form a pervaporation membrane, including a low-temperature photoreaction tank and a high-temperature film-forming tank. When the aloe polysaccharide liquid reacts in the low-temperature photoreaction tank, the aloe polysaccharide pervaporation membrane can be obtained in the high-temperature film-forming tank.

Description

Technical Field

[0001] The invention relates to the technical field of biomaterials, in particular to the field of natural polymers, biomembranes and pervaporation membrane preparation technology, and in particular to a method for promoting aloe polysaccharide to form a pervaporation membrane. Background Art

[0002] Pervaporation membrane technology is a new type of membrane separation technology. This membrane technology is different from other membranes and has special properties for screening substances. This technology is often used to separate liquid mixtures. Its outstanding advantage is that it can achieve separation tasks that are difficult to complete with traditional methods such as distillation, extraction, and adsorption with low energy consumption. It is particularly suitable for the separation of near-boiling point and constant boiling point mixtures that are difficult or impossible to separate by ordinary distillation; it has obvious economic and technical advantages in removing trace water from organic solvents and mixed solvents, and separating small amounts of organic pollutants in wastewater; it can also be coupled with chemical reactions to continuously remove reaction products, thereby significantly improving the reaction conversion rate.

[0003] Although a variety of separation membranes have been industrialized, few existing membrane-based separation processes can meet green environmental protection requirements. A large amount of toxic substances are used in membrane processes, which requires a lot of downstream treatment. In addition, most polymers used in membrane manufacturing, such as polyethersulfone (PES), polysulfone (PSf), polyvinylidene fluoride (PVDF) and polyamide (PA), are petrochemical-based polymers. In recent years, global attention has been increasing on polysaccharide-based polymers, not only to replace petrochemical-based polymers, but also as promising materials to develop innovative biotechnology. Different types of polysaccharides, such as cellulose, starch, chitin, alginate and chitosan-based carbohydrate polymers have been applied to the manufacture of pervaporation membranes. Pervaporation membranes naturally produced during the evaporation of aloe polysaccharide liquid from natural aloe polysaccharide liquid have not been reported so far.

[0004] Humans have been looking for ways to make wounds heal into regenerated skin rather than scar tissue. Scar tissue has obvious skin function defects compared to regenerated skin, such as loss of skin texture, abnormal perception, loss of hair follicles, etc. Whether hair follicles can regenerate is an important indicator of whether wounds heal into regenerated skin.

[0005] Aloe vera has a long history of application in wound repair. Aloe polysaccharide is the main active ingredient of aloe vera. However, looking around the world, current aloe polysaccharide extracts have not shown a significant function in promoting wound repair and have not been developed into medicine. Summary of the invention

[0006] The purpose of the present invention is to provide a method for promoting aloe polysaccharide to form a pervaporation membrane, aiming to provide a method for promoting aloe polysaccharide to form a pervaporation membrane with a simple preparation procedure and low cost.

[0007] To achieve the above-mentioned purpose, the method for promoting aloe polysaccharide to form a pervaporation membrane proposed in the present invention adopts a device for promoting aloe polysaccharide to form a pervaporation membrane, the device for promoting aloe polysaccharide to form a pervaporation membrane comprises: a low-temperature light reaction tank and a high-temperature film-forming tank, the low-temperature light reaction tank comprises a lamp, a stirring tank and a stirrer, the stirrer comprises a stirring motor, a stirring shaft and a stirring paddle, the stirring paddle is arranged at intervals on the stirring shaft, and the stirring shaft is connected to the stirring motor;

[0008] The aloe polysaccharide liquid is placed in the stirring tank, the temperature of the aloe polysaccharide liquid in the stirring tank is controlled at 4 to 15 degrees Celsius, the lamp is irradiated on the stirring tank, the stirring motor drives the stirring paddle to stir the aloe polysaccharide liquid, and the aloe polysaccharide liquid obtained after treatment is placed in the high-temperature film-forming tank, the temperature of the aloe polysaccharide liquid in the high-temperature film-forming tank is controlled at 75 to 95 degrees Celsius, the high-temperature film-forming tank adopts an inclined bottom, and the aloe polysaccharide permeation vaporization membrane is generated on the surface of the aloe polysaccharide liquid in the high-temperature film-forming tank.

[0009] Optionally, the stirring tank adopts an ellipsoidal bottom, and the stirring tank includes a cylindrical section and a spherical bottom section.

[0010] Optionally, the height of the cylindrical section is H, the length of the stirring shaft is L, and H=L.

[0011] Optionally, the illumination of the lamp at the illumination monitoring point in the mixing tank is not less than 800 lumens.

[0012] Optionally, the reaction time of the aloe polysaccharide liquid in the stirring tank is at least 24 hours.

[0013] Optionally, the high-temperature film-forming tank is an open tank with a sloping bottom, the diameter of the open tank is D1, the height of the short side of the open tank is DL, the height of the long side of the open tank is DR, D1≤DL, DR≥1.1DL.

[0014] In the technical solution of the present invention, the stirring motor drives the stirring paddle to stir the aloe polysaccharide liquid, the lamp irradiates the aloe polysaccharide liquid with a specific illumination, and maintains the temperature of the aloe polysaccharide liquid under low temperature conditions within a certain range for a certain period of time, the aloe polysaccharide liquid treated in the low-temperature light reaction tank is placed in the high-temperature film-forming tank, and the aloe polysaccharide liquid is maintained under high temperature conditions within a certain range. It can be observed that aloe polysaccharide pervaporation membrane is continuously generated on the surface of the aloe polysaccharide liquid; and the aloe polysaccharide pervaporation membrane has significant biological activity differences with the aloe polysaccharide liquid, and can significantly promote the regeneration of hair follicles in wounds. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without paying creative work.

[0016] Figure 1 It is a schematic diagram of the structure of a device for promoting aloe polysaccharide to form a pervaporation membrane;

[0017] Figure 2 This is a photo of the aloe polysaccharide pervaporation membrane on the surface of the aloe polysaccharide liquid;

[0018] Figure 3 This is a scanning electron micrograph of aloe polysaccharide solution (ABPA1);

[0019] Figure 4 This is a scanning electron micrograph of the aloe polysaccharide pervaporation membrane (ABPA2);

[0020] Figure 5 This is a comparison chart of the effects of aloe polysaccharide solution and aloe polysaccharide pervaporation membrane on HE-stained wound tissue sections and the number of hair follicles in scalded rats.

[0021] Description of Figure Numbers:

[0022]

[0023] The realization of the purpose, functional features and advantages of the present invention will be further explained in conjunction with embodiments and with reference to the accompanying drawings. DETAILED DESCRIPTION

[0024] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0025] It should be noted that all directional indications (such as up, down, left, right, front, back, etc.) in the embodiments of the present invention are only used to explain the relative position relationship, movement status, etc. between the components under a certain specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indication will also change accordingly.

[0026] In addition, the descriptions of "first", "second", etc. in the present invention are only used for descriptive purposes and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In addition, the technical solutions between the various embodiments can be combined with each other, but they must be based on the ability of ordinary technicians in the field to implement them. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such a combination of technical solutions does not exist and is not within the scope of protection required by the present invention.

[0027] In one embodiment of the present invention, see Figure 1 , Figure 1 The device 1000 for promoting aloe polysaccharide to form a pervaporation membrane is shown in the figure. The device is composed of a low-temperature photoreaction tank 1 and a high-temperature film-forming tank 2, and the corresponding dimensions (the distance from the lamp 11 to the stirring tank 12 is G, the diameter of the high-temperature film-forming tank 2 is D1, the short side height of the high-temperature film-forming tank 2 is DL, the long side height of the high-temperature film-forming tank 2 is DR, the height of the cylindrical section 121 is H, the height of the ball bottom section 122 is S, the length of the stirring shaft 132 is L, and the diameter of the stirring tank 12 is D): G=80 cm, H=D=L=120 cm, S=22 cm, DL=D1=120 cm, DR=132 cm, the aloe polysaccharide liquid is placed in the stirring tank 12, the lamp 11 is turned on to irradiate the aloe polysaccharide liquid, the stirring motor 131 is turned on at a speed of 80 rpm, and the aloe polysaccharide liquid is fully flowed and fully reacted for at least 24 hours.

[0028] The aloe polysaccharide liquid after the reaction in the low temperature photoreactor 1 is pumped into the high temperature film forming tank 2. It can be observed that within 72 hours, the aloe polysaccharide pervaporation membrane is continuously generated on the surface of the aloe polysaccharide liquid, and the steam is continuously vaporized through the membrane, and the aloe polysaccharide liquid is concentrated. The effect of the aloe polysaccharide pervaporation membrane after film formation is shown in the figure. Figure 2 It is worth pointing out that Figure 2 In order to reflect the actual film-forming state and color of the aloe polysaccharide pervaporation membrane, it is necessary to use color drawings to show it. Figure 2 When a black and white image is used, the film-forming state and color of the aloe polysaccharide pervaporation membrane will become petroleum-like, which is totally inconsistent with the film-forming state of the aloe polysaccharide pervaporation membrane.

[0029] The high-temperature film-forming tank 2 adopts an inclined bottom design which helps to evenly distribute the aloe polysaccharide liquid in the tank. When the aloe polysaccharide liquid enters the high-temperature film-forming tank 2, the inclined bottom structure makes the evaporation rate of the long side higher than that of the short side, thereby generating fluid dynamics to make the liquid form a directional flow toward the long side of the surface of the material body, thereby promoting the formation of aloe polysaccharide pervaporation membrane; under the same other conditions, the yield of the aloe polysaccharide liquid treated by the low-temperature light reactor 1 placed in a round-bottom or flat-bottom tank is significantly lower than that in a inclined-bottom tank. If it is in a negative-pressure high-temperature film-forming tank 2, no aloe polysaccharide pervaporation membrane can be generated, and the obtained product is observed by scanning electron microscopy (such as Figure 3 as shown), and does not possess membrane properties.

[0030] In one embodiment of the present invention, obtaining the aloe polysaccharide solution includes the following process steps:

[0031] Remove both ends of the aloe vera leaf and soak it in pure water for 1 to 2 days; wash it with water and remove the epidermis to obtain the aloe vera gel to be ground.

[0032] The aloe vera gel to be ground is poured into the automatic gel grinding device for producing aloe vera gel; the shear motor rotates clockwise at a speed of 1450 r / min; after 15 to 20 minutes, the gel grinding is completed to obtain the aloe polysaccharide liquid.

[0033] In this embodiment, the grinding method is used instead of the crushing method, the purpose of which is to stimulate the parenchyma cells of aloe to secrete more polysaccharides through friction, so as to accelerate the release of polysaccharides during the extraction process and improve the extraction efficiency.

[0034] The aloe polysaccharide solution and the aloe polysaccharide pervaporation membrane were imaged by scanning electron microscope. It can be observed from the images that compared with the aloe polysaccharide solution (see Figure 3 and Figure 4 ), the aloe polysaccharide pervaporation membrane has shown planar continuity at the microscopic level, that is, membrane characteristics.

[0035] The aloe polysaccharide pervaporation membrane material was applied to the scald wound of SD rats, and the tissue was HE stained and sectioned 28 days after the wound modeling. The results are shown in the following table (Table 1). Compared with the control group (CONTROL), please refer to Figure 5 (The horizontal axis of the right figure represents two different experimental groups, CONTROL represents SD rats coated with aloe polysaccharide liquid, AVBER represents SD rats coated with aloe polysaccharide pervaporation membrane, and the vertical axis of the right figure represents the number of hair follicles of SD rats, and three stars represent statistically significant differences). The hair follicle number (Hair Follicle Number) of SD rats (ABPA2) coated with the aloe polysaccharide pervaporation membrane material was significantly improved, that is, the aloe polysaccharide pervaporation membrane has a significant effect on the repair of hair follicles.

[0036] Table 1: Results of the number of hair follicles of SD rats smeared with aloe polysaccharide pervaporation membrane and the number of hair follicles of SD rats smeared with aloe polysaccharide liquid:

[0037]

[0038] In one embodiment of the present invention, the stirring tank 12 adopts an ellipsoid bottom, and the temperature of the aloe polysaccharide liquid in the stirring tank 12 is controlled at 4 to 15 degrees Celsius. A jacket circulation system is usually used to control the temperature of the aloe polysaccharide liquid in the stirring tank 12. The jacket circulation system cools it outside the stirring tank 12. The jacket is a closed space installed outside the stirring tank 12. A heat conduction channel is formed between it and the wall of the stirring tank 12. By circulating a cooling medium (such as alcohol and cooling water, etc.) in this channel, the aloe polysaccharide liquid inside the stirring tank 12 can be cooled, thereby maintaining the temperature of the aloe polysaccharide liquid within the range of 4 to 15 degrees Celsius. The stirring tank 12 adopts an ellipsoid bottom design, mainly to promote the circulation and mixing of the aloe polysaccharide liquid. The ellipsoid bottom design can reduce dead angles, so that the aloe polysaccharide liquid is more easily stirred evenly by the stirrer 13, reduce the local flow rate difference during the mixing process, and improve the mixing efficiency. In addition, the ellipsoid bottom can reflect light to the liquid at multiple angles to improve the illumination efficiency.

[0039] In one embodiment of the present invention, the illumination of the lamp 11 at the illumination monitoring point 3 in the stirring tank 12 should not be less than 800 lumens. In order to monitor the illumination at the illumination monitoring point 3, a light monitoring component is set at the illumination monitoring point 3. The light monitoring component can be a photodiode light sensor or a photoresistor light sensor. The present invention does not limit this. The illumination monitored by the light monitoring component cannot be less than 800 lumens, which can ensure that the photochemical reaction of the aloe polysaccharide liquid has sufficient energy input.

[0040] In the present invention, low temperature treatment helps to maintain the stability and activity of the aloe polysaccharide liquid, reduce its degradation or denaturation, and slow down the degradation rate of the aloe polysaccharide liquid. Stirring can ensure that the components in the aloe polysaccharide liquid are evenly distributed, and form sufficient vortices to reduce local concentration differences, and can also allow the aloe polysaccharide liquid to be evenly illuminated and evenly contacted with air, thereby avoiding local degradation of the aloe polysaccharide liquid and providing better conditions for the subsequent membrane pervaporation process.

[0041] Appropriate light can help trigger or accelerate the photochemical reaction in aloe polysaccharide solution. When aloe polysaccharide solution is reacted for at least 24 hours under low temperature, stirring and light, the degree of hydrolysis of aloe polysaccharide solution is higher and the viscosity is lower (at room temperature of 20 degrees Celsius, the viscosity of aloe polysaccharide solution without light treatment and aloe polysaccharide solution with light treatment was tested and found that the viscosity of aloe polysaccharide solution without light treatment was 280.25mpa·s, and the viscosity of aloe polysaccharide solution with light treatment was 135.00mpa·s. Obviously, light can reduce the viscosity of aloe polysaccharide solution).

[0042] In one embodiment of the present invention, the temperature of the aloe polysaccharide liquid in the high temperature film forming tank 2 is controlled at 75 to 95 degrees Celsius. A jacket circulation system is usually used to control the temperature of the aloe polysaccharide liquid in the high temperature film forming tank 2. The jacket circulation system heats the aloe polysaccharide liquid outside the high temperature film forming tank 2. The jacket is a closed space installed outside the high temperature film forming tank 2. A heat conduction channel is formed between the jacket and the wall of the high temperature film forming tank 2. By circulating a heating medium (such as heat transfer oil and water, etc.) in this channel, the aloe polysaccharide liquid inside the high temperature film forming tank 2 can be heated, thereby maintaining the temperature of the aloe polysaccharide liquid within the range of 75 to 95 degrees Celsius, so as to continuously form an aloe polysaccharide pervaporation membrane in the high temperature film forming tank 2.

[0043] The above description is only a preferred embodiment of the present invention, and does not limit the patent scope of the present invention. All equivalent structural changes made by using the contents of the present invention specification and drawings under the inventive concept of the present invention, or directly / indirectly applied in other related technical fields are included in the patent protection scope of the present invention.

Claims

1. A method for promoting aloe polysaccharide to form a pervaporation membrane, characterized in that: The method adopts a device (1000) for promoting aloe polysaccharide to form a pervaporation membrane, wherein the device (1000) for promoting aloe polysaccharide to form a pervaporation membrane comprises a low-temperature light reaction tank (1) and a high-temperature film-forming tank (2), wherein the low-temperature light reaction tank (1) comprises a lamp (11), a stirring tank (12) and a stirrer (13), wherein the stirrer (13) comprises a stirring motor (131), a stirring shaft (132) and a stirring paddle (133), wherein the stirring paddle (133) is arranged at intervals on the stirring shaft (132), and the stirring shaft (132) is connected to the stirring motor (131); The aloe polysaccharide liquid is placed in the stirring tank (12), and the temperature of the aloe polysaccharide liquid in the stirring tank (12) is controlled at 4 to 15 degrees Celsius. The lamp (11) irradiates the stirring tank (12), and the stirring motor (131) drives the stirring paddle (133) to stir the aloe polysaccharide liquid. The aloe polysaccharide liquid obtained after treatment is placed in the high-temperature film-forming tank (2), and the temperature of the aloe polysaccharide liquid in the high-temperature film-forming tank (2) is controlled at 75 to 95 degrees Celsius. The high-temperature film-forming tank (2) adopts an inclined bottom, and the aloe polysaccharide permeation vaporization membrane is generated on the surface of the aloe polysaccharide liquid in the high-temperature film-forming tank (2).

2. The method for promoting aloe polysaccharide to form a pervaporation membrane as claimed in claim 1, characterized in that: The stirring tank (12) adopts an ellipsoidal bottom, and the stirring tank (12) comprises a cylindrical section (121) and a spherical bottom section (122).

3. The method for promoting aloe polysaccharide to form a pervaporation membrane as claimed in claim 2, characterized in that: The height of the cylindrical section (121) is H, the length of the stirring shaft (132) is L, and H=L.

4. The method for promoting aloe polysaccharide to form a pervaporation membrane as claimed in claim 1, characterized in that: The illumination of the lamp (11) at the illumination monitoring point (3) in the mixing tank (12) is not less than 800 lumens.

5. The method for promoting aloe polysaccharide to form a pervaporation membrane as claimed in claim 1, characterized in that: The reaction time of the aloe polysaccharide liquid in the stirring tank (12) is at least 24 hours.

6. The method for promoting aloe polysaccharide to form a pervaporation membrane as claimed in claim 1, characterized in that: The high-temperature film-forming tank (2) is an open tank with an inclined bottom, the diameter of the open tank is D1, the height of the short side of the open tank is DL, the height of the long side of the open tank is DR, D1≤DL, DR≥1.1DL.

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