Composition for absorbing grease and promoting defecation, preparation method and application thereof
By combining cassia seeds, sorbitol, rohan fruit powder, aloe vera powder, orange peel powder and inulin, a composition that absorbs fat is prepared, which solves the problem of insufficient complexity and stability of food ingredients for constipation treatment, and achieves the Efficient intestinal moisturization and promote intestinal peristalsis, improving the stability and taste of the product.
Patent Information
- Application Number
- CN202411004242.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2024-07-25
- Publication Date
- 2024-12-13
- Estimated Expiration
- 2044-07-25
AI Technical Summary
In the prior art, the food ingredients used to treat constipation are too complex, which can easily lead to intestinal flora disorders, and the stability and uniformity of highly absorbent ingredients such as cassia seeds and orange peel powder are insufficient, which affects the storage period and use of the product. Effect.
Cassia seeds, sorbitol, Luohan fruit powder, aloe vera powder, orange peel powder and inulin are prepared to absorb oil and promote defecation. The stability and taste of the composition are improved by the preparation of Cassia seed composite particles. And use sorbitol to reduce water activity and microbial growth.
Improve the uniformity and stability of the composition, significantly improve the effects of moistening the intestines and promoting intestinal peristalsis, prolong the shelf life, reduce the moisture activity of the product, avoid microbial growth, improve taste and fluidity .
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Figure CN118525962B_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the field of food technology, and specifically relates to a composition for absorbing grease and promoting defecation, and a preparation method and application thereof. Background Art
[0002] Constipation is one of the more common sub-health conditions in modern times. According to traditional Chinese medicine, constipation is the result of large intestine dysfunction, which is mainly manifested in the following aspects: (1) Spleen deficiency and dysfunction, which is mostly caused by improper diet, long-term sitting and lack of exercise; (2) Heat in the stomach, constipation, dry and burning anus, bad breath, dry mouth, red tongue and rapid pulse; (3) Kidney deficiency and constipation; (4) Lung qi stagnation. The lung and large intestine are the exterior and interior of each other. The lung qi is responsible for the rapid descent of the internal organs and is yin. The large intestine is responsible for the excretion of waste and is yang. It is normal for yin to rise and yang to descend. If external evil restrains the exterior and the lung qi fails to circulate, the visceral qi cannot flow and descend, and dry and hard stools will occur.
[0003] The most direct and simple treatment for constipation is to use laxatives, but long-term use of laxatives can cause abdominal pain, stress, megacolon, and even drug resistance. Although surgery, traditional Chinese medicine, acupuncture, etc. also have certain improvements and therapeutic effects on constipation, they still have limitations and are not suitable for large-scale treatment. Constipation can also occur due to improper diet. Due to the lack of mechanical or chemical stimulation caused by food, insufficient intestinal motility leads to constipation. Therefore, while eating a healthy diet, eating foods that promote intestinal motility can promote defecation and relieve constipation.
[0004] Chinese invention patent CN103431376A discloses a special diet for people with constipation, comprising the following components: hemp seed, Prunus mume seed, radish seed, cassia seed, mulberry, polygonatum, lily, resistant dextrin, astragalus pollen, barley seed, lotus leaf, hawthorn, monk fruit, natto, pine pollen, sophora japonica flower, yam, buckwheat, tangerine peel, yeast, soybean dietary fiber, chrysanthemum and Panax notoginseng, silkworm pupae, Lactobacillus casei, Streptococcus thermophilus, oligoxylose, L-arabinose, spirulina, konjac, sweet potato, corn pollen, taurine, Lactobacillus acidophilus, Bifidobacterium, milk alkaline protein, fish oil extract, aloe vera gel, marine fish oligopeptide powder and black sesame; the diet is mainly composed of various probiotics, which regulate the balance of intestinal microecology, enhance the motility of the spleen and stomach, and reduce the development of gastroenteritis-related diseases. However, the above diet uses a large amount of ingredients that promote intestinal motility, and the ingredients are too complex. The use of a large amount of bacteria will aggravate intestinal flora disorders.
[0005] Chinese invention patent CN107183688A provides a plant compound enzyme, including main ingredients and auxiliary ingredients, the main ingredients are at least four selected from cassia seed, prunes, brown rice, coix seed, black fungus, hemp seed, polygonatum, wolfberry, black rice and sesame; auxiliary ingredients include konjac flour, Jerusalem artichoke flour, soy protein, whey protein powder, citrus fiber, oat bran and hawthorn powder. The main raw materials of the plant compound enzyme are fermented and processed, and are matched with other beneficial raw materials, which has the effects of moisturizing the intestines, promoting bowel movements, and clearing the intestines and detoxifying. However, the enzyme may cause bacterial growth after consumption, leading to diarrhea, abdominal pain, loss of appetite, etc. In severe cases, it may also induce skin or gastrointestinal diseases.
[0006] In addition, ingredients such as cassia seed and tangerine peel that have laxative effects have high hygroscopicity during use, thus affecting the stability and uniformity of the product; when using powders of ingredients such as cassia seed and tangerine peel, they need to be specially treated to improve the storage period and stability of the material.
[0007] Based on this, it is necessary to study a food composition that has no chemical intervention, has not been fermented by bacteria, has high storage stability, and has the effects of moisturizing the intestines and promoting bowel movements. Summary of the invention
[0008] The present invention aims at the problems existing in the prior art, and provides a composition for absorbing grease and promoting defecation, and a preparation method and application thereof, wherein cassia seed, sorbitol, monk fruit powder, aloe powder, orange peel powder, inulin and maltitol are matched with each other, and the obtained composition has significant effects in moistening the intestine and promoting bowel movements, and has high uniformity and stability; and the present invention prepares cassia seed composite particles by combining cassia seed and sorbitol, which can not only improve the taste and flavor of the composition, but also improve gastrointestinal peristalsis. The composition obtained by the present invention is applied to the preparation of food having the functions of absorbing grease, promoting defecation and promoting gastrointestinal peristalsis.
[0009] To achieve the above purpose, the technical solution adopted by the present invention is as follows:
[0010] Firstly, the present invention provides a composition for absorbing oil and promoting defecation, which comprises the following components in parts by weight: 5-30 parts of cassia seeds, 5-30 parts of sorbitol, 1-10 parts of monk fruit powder, 1-10 parts of aloe powder, 5-20 parts of orange peel powder, 5-30 parts of inulin and 5-30 parts of maltitol.
[0011] Preferably, the composition comprises the following components, in parts by weight: 8-20 parts of cassia seeds, 8-20 parts of sorbitol, 3-8 parts of monk fruit powder, 3-8 parts of aloe powder, 10-15 parts of orange peel powder, 10-20 parts of inulin and 12-20 parts of maltitol.
[0012] Further preferably, the composition comprises the following components, in parts by weight: 15 parts of cassia seeds, 15 parts of sorbitol, 6 parts of monk fruit powder, 6 parts of aloe powder, 12 parts of orange peel powder, 15 parts of inulin and 16 parts of maltitol.
[0013] Preferably, the aloe vera powder is aloe vera gel powder.
[0014] Preferably, the particle size of the orange peel powder is 50-80 mesh, and the particle size of the inulin is 50-80 mesh; further preferably, the particle size of the orange peel powder is 60 mesh, and the particle size of the inulin is 60 mesh.
[0015] Preferably, the composition further comprises flavoring agents; the flavoring agents are essences and salts.
[0016] Further preferably, in the flavoring agent, the weight ratio of essence to salt is 0.05-0.2:0.1-5.
[0017] Furthermore, the present invention provides a method for preparing the above composition, comprising the steps of:
[0018] (1) granulating a formula amount of Cassia seed and 5-15% of a formula amount of sorbitol to obtain Cassia seed composite particles; then mixing the Cassia seed composite particles with a formula amount of aloe powder to obtain a mixture A;
[0019] (2) mixing a formulated amount of monk fruit powder and a formulated amount of inulin to obtain a mixture B;
[0020] (3) mixing the formulated amount of orange peel powder with the remaining formulated amount of sorbitol, granulating the mixture, and sieving the mixture to obtain a mixture C;
[0021] (4) mixing mixture A and a formulated amount of maltitol to obtain mixture 1;
[0022] (5) Mixing mixture B and mixture C to obtain mixture 2;
[0023] (6) Mixture 1 and mixture 2 are mixed to obtain a composite material.
[0024] Preferably, in step (1), the preparation steps of the Cassia seed composite particles are: preparing sorbitol into a sorbitol liquid with a mass concentration of 10-45%; crushing the formulated amount of Cassia seeds, mixing with the sorbitol liquid and feeding, and granulating under the conditions of an air inlet volume of 10-24 Hz, a slurrying speed of 10-32 rpm, a material temperature of 39-46°C, an air outlet temperature of 30-52°C, and a pressure of 0.1-0.4 MPa to obtain the Cassia seed composite particles.
[0025] Further preferably, the preparation steps of the Cassia seed composite particles are: preparing sorbitol into a sorbitol liquid with a mass concentration of 15-20%; crushing the formulated amount of Cassia seeds and adding them to a fluidized bed granulator, spraying them into the sorbitol liquid, and granulating under the conditions of an air inlet volume of 10-20 Hz, a slurry adding speed of 10-25 rpm, a material temperature of 39-46°C, an air outlet temperature of 30-45°C, and a pressure of 0.1-0.4 MPa, and controlling the moisture content of the particles to be 1-2.5%, to obtain Cassia seed composite particles.
[0026] More preferably, the Cassia seed is crushed to a particle size of 50-80 mesh; more preferably, the crushed particle size is 60 mesh.
[0027] More preferably, during the preparation of the Cassia seed composite particles, the moisture content of the particles is controlled at 1.8-2.1%.
[0028] Preferably, in step (1), the mixing is specifically: stirring at a rotation speed of 5-20 rpm for 15-25 min; further preferably, the mixing is specifically: stirring at a rotation speed of 10 rpm for 20 min.
[0029] Preferably, in step (2), the mixing is specifically: stirring at a rotation speed of 5-20 rpm for 15-30 min; further preferably, the mixing is specifically: stirring at a rotation speed of 10 rpm for 25 min.
[0030] Preferably, step (3) is specifically as follows: the formulated amount of orange peel powder and the remaining formulated amount of sorbitol are stirred at a rotation speed of 5-20 rpm for 5-15 minutes to obtain a mixed powder, the mixed powder is placed in a granulator, the feeding speed is 3-30 rpm, the roller speed is 2-15 rpm, the roller pressure is 10-60 bar, the roller gap is 2-6 mm, the primary granulation screen is 10-20 mesh, the secondary finishing screen is 20-60 mesh, and the granulation cutter speed is 20-100 rpm to obtain a mixture C.
[0031] Further preferably, step (3) is specifically as follows: the formulated amount of orange peel powder and the remaining formulated amount of sorbitol are stirred at a speed of 10 rpm for 10 minutes to obtain a mixed powder, the mixed powder is placed in a granulator, the feeding speed is 10-20 rpm, the roller speed is 8-12 rpm, the roller pressure is 25-40 bar, the roller gap is 3-5 mm, the primary granulation screen is 10-20 mesh, the secondary finishing screen is 30-40 mesh, and the granulation cutter speed is 50-80 rpm to obtain a mixture C.
[0032] More preferably, step (3) is specifically as follows: the formulated amount of orange peel powder and the remaining formulated amount of sorbitol are stirred at a speed of 10 rpm for 10 minutes to obtain a mixed powder, the mixed powder is placed in a granulator, the feeding speed is 15 rpm, the roller speed is 10 rpm, the roller pressure is 30 bar, the roller gap is 4 mm, the primary granulation screen is 15 mesh, the secondary finishing screen is 40 mesh, and the granulation cutter speed is 60 rpm to obtain a mixture C.
[0033] Preferably, in step (4) and step (5), the mixing is specifically: stirring at a rotation speed of 5-20 rpm for 10-25 min; further preferably, the mixing is specifically: stirring at a rotation speed of 10 rpm for 15 min.
[0034] Preferably, in step (6), the mixing is specifically: stirring at a rotation speed of 5-20 rpm for 15-30 min; further preferably, the mixing is specifically: stirring at a rotation speed of 10 rpm for 25 min.
[0035] Preferably, when the composition contains a flavoring component, a formulated amount of the flavoring is added in step (6) and mixed with mixture 1 and mixture 2 to obtain a composition.
[0036] Finally, the present invention provides the use of the above composition in preparing food having the functions of absorbing fat, promoting defecation and promoting gastrointestinal motility.
[0037] Compared with the prior art, the present invention has the following beneficial effects:
[0038] 1. The present invention mixes cassia seed, monk fruit powder, aloe powder, tangerine peel powder, inulin, sorbitol and maltitol separately to improve the uniformity of the composition product; at the same time, the composition of the present invention has high activity, high stability and a long shelf life; mixing cassia seed and tangerine peel powder with sorbitol separately can not only improve the ovulation-promoting effect of the composition, but also improve the taste.
[0039] 2. The present invention uses sorbitol to maintain the stability of the composition, reduce the water activity of the product, and limit the growth and reproduction of microorganisms; at the same time, sorbitol has a chelating effect, combines with metal ions to form chelates, thereby maintaining internal moisture and preventing metal ions from combining with enzymes, reducing the activity of proteases, and improving stability; sorbitol as a sweetener can also mask the bitter taste brought by Cassia seed itself, remain in the porous structure of Cassia seed powder, keep Cassia seed traditional Chinese medicine powder stable, and inhibit the growth of microorganisms.
[0040] 3. The composition of the present invention has significant effects in moistening the intestines and promoting bowel movements, and has high edible and application value.
[0041] 4. In the composition of the present invention, sorbitol and orange peel powder are used in combination with each other to reduce the electrostatic effect of orange peel powder and improve the fluidity and uniformity of the composition. BRIEF DESCRIPTION OF THE DRAWINGS
[0042] Figure 1 This is a fluorescence intensity comparison chart of the ovulation-promoting effect of Cassia seed composite particles, sorbitol, and Cassia seed powder in Example 2.
[0043] Figure 2 It is a comparison chart of the fluorescence intensity of the ovulation-promoting effect of the composition of Example 2 at different dilution concentrations and the blank control group. DETAILED DESCRIPTION
[0044] The following non-limiting examples can enable those skilled in the art to more fully understand the present invention, but do not limit the present invention in any way. The following content is merely an exemplary description of the scope of the present invention, and those skilled in the art can make various changes and modifications to the present invention based on the disclosed content, which should also fall within the scope of the present invention.
[0045] When the embodiments give numerical ranges, it should be understood that, unless otherwise specified in the present invention, both endpoints of each numerical range and any numerical value between the two endpoints can be selected. Unless otherwise defined, all technical and scientific terms used in the present invention have the same meanings as those generally understood by those of ordinary skill in the art to which the present invention belongs.
[0046] The present invention is further described below by way of specific examples. Unless otherwise specified, the various chemical reagents used in the examples of the present invention are obtained through conventional commercial channels.
[0047] In the following examples, the monk fruit powder was purchased from Guilin Jifusi Monk Fruit Co., Ltd., item number MFC-E13.5; the aloe vera powder was aloe vera gel powder purchased from Yunnan Wanlv Biological Co., Ltd., 0806-2 (model 100:1); the orange peel powder was purchased from Shaanxi Huike Plant Development Co., Ltd., HK20221108; the inulin was purchased from BENEO-Orafti, item number RRYSX1ASX1; the sorbitol was purchased from Roquette, item number 100T; the maltitol was purchased from Shandong Bailong, model 50. The above components, products from different manufacturers have no significant effect on the effect.
[0048] Example 1-Example 5
[0049] A composition for absorbing grease and promoting defecation, as shown in Table 1, is composed of the following components in parts by weight:
[0050] Table 1
[0051]
[0052] The preparation method of Cassia seed composite particles is as follows: taking 10% of the formula amount of sorbitol, preparing it into a sorbitol liquid with a mass concentration of 20%; crushing the Cassia seed to a particle size of 60 mesh, then adding it to a fluidized bed granulator, spraying it into the sorbitol liquid with a mass concentration of 20%, granulating under the conditions of an air inlet volume of 10-20Hz, a slurry adding speed of 10-25rpm, a material temperature of 39-46℃, an air outlet temperature of 30-45℃, and a pressure of 0.1-0.4MPa, and controlling the moisture content of the particles to 2.1%, to obtain Cassia seed composite particles. The prepared Cassia seed composite particles are slightly sweet and have no bitter taste.
[0053] In Examples 1 to 5, the preparation method of the composition is:
[0054] (1) Stirring the Cassia seed composite particles and the formulated amount of aloe vera powder at 10 rpm for 20 min to obtain a mixture A;
[0055] (2) stirring the formula amount of monk fruit powder and the formula amount of inulin with a particle size of 60 mesh at a speed of 10 rpm for 25 minutes to obtain a mixture B;
[0056] (3) The orange peel powder with a particle size of 60 mesh and the remaining amount of sorbitol were stirred at a speed of 10 rpm for 10 min, and the obtained mixed powder was placed in a granulator with a feeding speed of 15 rpm, a roller speed of 10 rpm, a roller pressure of 30 bar, a roller gap of 4 mm, a primary granulation screen of 15 mesh, a secondary finishing screen of 40 mesh, and a granulation cutter speed of 60 rpm to obtain a mixture C;
[0057] (4) Mixing mixture A and the formulated amount of maltitol were stirred at a speed of 10 rpm for 15 minutes to obtain mixture 1;
[0058] (5) Mixture B and mixture C were mixed and stirred at a speed of 10 rpm for 15 min to obtain mixture 2;
[0059] (6) Mix the formulated amount of flavoring agent, mixture 1 and mixture 2 at a rotation speed of 10 rpm for 25 minutes to obtain a composition.
[0060] The compositions prepared in the above Examples 1-5 have appropriate sweetness, moderate sweetness and sourness, no peculiar smell, no obvious granularity, good solubility, uniform color, and excellent flavor and taste.
[0061] Example 6
[0062] The difference from Example 2 is that the particle sizes of orange peel powder and inulin are both 80 meshes. The rest are the same as Example 2.
[0063] Comparative Example 1
[0064] Different from Example 2, the preparation of Cassia seed composite particles was not performed;
[0065] (1) After the formulated amount of Cassia seeds is crushed into 60 mesh, it is mixed with the formulated amount of aloe powder at a speed of 10 rpm for 20 minutes to obtain a mixture A;
[0066] (2) Mixing the formulated amount of monk fruit powder and the formulated amount of inulin with a particle size of 60 meshes at a speed of 10 rpm for 25 minutes to obtain a mixture B;
[0067] (3) The orange peel powder with a particle size of 60 mesh and the sorbitol with a particle size of 40 mesh were mixed at a speed of 10 rpm for 10 min. The obtained mixed powder was placed in a granulator with a feeding speed of 15 rpm, a roller speed of 10 rpm, a roller pressure of 30 bar, a roller gap of 4 mm, a primary granulation screen of 15 mesh, a secondary finishing screen of 40 mesh, and a granulation cutter speed of 60 rpm to obtain a mixture C.
[0068] Steps (4) to (6) are the same as in Example 2.
[0069] The stability and uniformity of the prepared composition are worse than those of Example 2.
[0070] Comparative Example 2
[0071] The difference from Example 2 is that Cassia seed is replaced by hemp seed to prepare hemp seed composite particles. The rest is the same as Example 2.
[0072] Comparative Example 3
[0073] The difference from Example 2 is that in step (1), the preparation method of Cassia seed composite particles is different, specifically:
[0074] Take 10% of the formula amount of sorbitol and prepare it into a sorbitol liquid with a mass concentration of 20%; crush the formula amount of Cassia seed to a particle size of 60 mesh, directly mix and stir evenly with the sorbitol liquid, dry, and control the moisture content of the particles to 2.1%, so as to obtain Cassia seed composite particles.
[0075] The remaining steps (2) to (6) are the same as those in Example 2.
[0076] Comparative Example 4
[0077] The difference from Example 2 is that the monk fruit powder is replaced by mulberry powder.
[0078] Comparative Example 5
[0079] Different from Example 2, the composition does not contain the component inulin.
[0080] Comparative Example 6
[0081] Different from Example 2, the composition does not contain aloe vera powder.
[0082] Comparative Example 7
[0083] The difference from Example 2 is that the weight ratio of each component in the composition is different.
[0084] The composition consists of the following components in parts by weight: 10 parts of cassia seeds, 15 parts of sorbitol, 14 parts of monk fruit powder, 11 parts of aloe powder, 15 parts of orange peel powder, 4 parts of inulin, 7 parts of maltitol and 1 part of flavoring agent.
[0085] Comparative Example 8
[0086] The difference from Example 2 is that the sorbitol component in the composition is replaced by xylitol.
[0087] The composition consists of the following components in parts by weight: 15 parts of cassia seeds, 15 parts of sorbitol, 6 parts of monk fruit powder, 6 parts of aloe powder, 12 parts of orange peel powder, 15 parts of inulin, 16 parts of xylitol and 1 part of flavoring agent.
[0088] Test 1 Sensory evaluation
[0089] 1. Test samples
[0090] The compositions prepared in Example 1, Example 2, Example 6, Comparative Example 1, Comparative Example 2 and Comparative Example 3 were brewed with warm water at 45° C. at a mass ratio of 1:8, and were fully stirred and then subjected to sensory evaluation.
[0091] The Cassia seed composite particles prepared in Example 1 and Comparative Example 3 and the hemp seed composite particles in Comparative Example 2 were brewed with warm water at 45° C. at a mass ratio of 1:8, and were fully stirred and then subjected to sensory evaluation.
[0092] 2. Sensory evaluation score
[0093] Twenty healthy volunteers aged 20-35 years were recruited, including 10 males and 10 females, who were not pregnant women or nursing mothers; had no other metabolic diseases, digestive system diseases, endocrine system diseases, mental illnesses, etc.; had no history of allergy or intolerance to the tested food; had not taken nutrient supplements that affect glucose tolerance, oral contraceptives, acetylsalicylic acid, steroids, protease inhibitors, antipsychotics and other drugs in the past 3 months.
[0094] Provide professional sensory evaluation training to volunteers and score the sensory evaluation of the above test samples.
[0095] The sensory evaluation scoring criteria are shown in Table 2.
[0096] Table 2
[0097]
[0098] 3. Sensory evaluation results
[0099] The results of sensory evaluation are shown in Table 3.
[0100] Table 3
[0101]
[0102] As can be seen from Table 3, the composition prepared by the present invention has a good sensory evaluation score. However, in Comparative Example 1, no Cassia seed composite particles were prepared, and the undesirable taste of Cassia seed, such as bitterness, was not improved, and the sensory evaluation score of the composition was low; although Comparative Example 3 used sorbitol to improve the sensory experience, Cassia seed was not prepared as encapsulated particles, and the sensory score was still poor.
[0103] Test 2 Evaluation of fluidity and uniformity
[0104] 1. Experimental methods
[0105] Bulk density test method: Bulk density refers to the ratio of the mass m of the diffuse powder to its filling volume v (including the gaps between the powders) when the powder is not vibrated. Pour the powder into a standard container and pile it up freely. Then measure the mass of the powder and the volume of the container and calculate ρ (bulk density) = m / v.
[0106] The tap density is tested using the bulk density and tap density determination methods of the 2020 edition of the Chinese Pharmacopoeia. Tap density test method: The powder sample in the container is vibrated downward at a specific frequency until the volume no longer changes, and the powder column is vibrated 200 times continuously at a vibration frequency of 50-60 times per minute.
[0107] Carr index: using ASTM D6393 international standard, reflects the compressibility of powder, Carr index (bulk density) = (tapped density - bulk density) / tapped density × 100%.
[0108] 2. Test samples
[0109] The compositions of Examples 1 to 6, the compositions of Comparative Examples 1 to 3, and the composition of Comparative Example 8.
[0110] The test results of each composition are shown in Table 4.
[0111] Table 4
[0112]
[0113] The raw materials such as orange peel powder and cassia seed have relatively poor fluidity, and the Carr index is greater than 25%, which easily causes uneven mixing of materials. In the present invention, sorbitol is added to cassia seed to prepare composite particles, and the Carr index is significantly reduced, the fluidity is significantly improved, and the mixing is more uniform. In the present invention, a specific amount of sorbitol is further added to the composition to improve the fluidity and uniformity of the composition.
[0114] Experiment 3 Evaluation of ovulation promotion effect
[0115] 1. Experimental methods
[0116] Zebrafish embryo collection: zebrafish (AB strain, a commonly used laboratory zebrafish strain obtained by early pressurization of haploid cells, purchased from Hangzhou Huante Biotechnology Co., Ltd.) were selected as broodstock, and the zebrafish were placed in a breeding tank at a ratio of female: male = 1:2, separated by a partition, and placed in a dark environment; the partition of the breeding tank was removed the next day and spawning began. Dye was added to the container of zebrafish 5 days after fertilization, and exposed overnight for 16 hours to completely fill the zebrafish intestine with dye. Normal 5-day-old zebrafish embryos were selected and placed in a six-well cell culture plate, 15 per well. The standard dilution water of the six-well plate was removed without harming the embryos, and the dilution of the test substance was quickly added to three wells, and incubated in the biochemical incubator in the dark for 45 hours.
[0117] The fluorescence intensity was observed under a fluorescence microscope, and the fluorescence intensity was calculated using Image J software.
[0118] 2. Test substances
[0119] ① The Cassia seed composite particles of Example 2 (dilution concentration 1.0 mg / mL), ② Sorbitol (dilution concentration 1.0 mg / mL), ③ Cassia seed powder (dilution concentration 1.0 mg / mL).
[0120] The composition of Example 2 (the dilution concentrations are 0.25 mg / mL, 0.5 mg / mL, and 1.0 mg / mL, respectively).
[0121] The composition of Example 1 (dilution concentration 1.0 mg / mL), the compositions of Examples 3 to 6 (dilution concentration 1.0 mg / mL), and the compositions of Comparative Examples 1 to 8 (dilution concentration 1.0 mg / mL).
[0122] Blank control group test substance: purified water.
[0123] 3. Results
[0124] Figure 1 This is a fluorescence intensity comparison chart of the induced ovulation effect of Cassia seed composite particles, sorbitol, and Cassia seed powder in Example 2. Figure 1A is the fluorescence intensity of the promoting effect of ovulation exhibited by the Cassia seed composite particles of Example 2; Figure 1 B is the fluorescence intensity of the promoting effect of sorbitol in Example 2; Figure 1 C is the fluorescence intensity of the ovulation-promoting effect shown by the Cassia seed powder in Example 2.
[0125] from Figure 1 It can be seen that after adding Cassia seed composite particles, the fluorescence intensity is significantly reduced, indicating that the Cassia seed composite particles have a significant effect in promoting ovulation.
[0126] Figure 2 The fluorescence intensity comparison diagram of the ovulation promoting effect of the composition of Example 2 at different dilution concentrations and the blank control group is shown in FIG. Figure 2 A is the fluorescence intensity of the ovulation-promoting effect of the composition of Example 2 at a dilution concentration of 0.25 mg / mL; Figure 2 B is the fluorescence intensity of the ovulation-promoting effect of the composition of Example 2 at a dilution concentration of 0.5 mg / mL; Figure 2 C is the fluorescence intensity of the ovulation-promoting effect of the composition of Example 2 at a dilution concentration of 1.0 mg / mL; Figure 2 D is the fluorescence intensity of the ovulation-promoting effect of the blank control group.
[0127] from Figure 2 It can be seen that when the dilution concentration of the composition of Example 2 is 1.0 mg / mL, the fluorescence intensity is significantly reduced, and it can be seen that the amount of dye discharged from the gastrointestinal tract is increased. The composition of the present invention has the effect of moisturizing the intestine and promoting bowel movements, and can reduce drug intake and chemical intervention.
[0128] The results of the intestinal fluorescence signal intensity and intestinal motility promoting effect (intestinal motility emptying rate) of each test substance are shown in Table 5. The " / " of the blank control group in Table 5 means that the effect of this group is used as the control, and the intestinal motility promoting effect of the blank control group is regarded as 0%.
[0129] Table 5
[0130]
[0131] As can be seen from Table 5, the compositions prepared in the examples of the present invention have a higher gastrointestinal emptying rate, and the intestinal emptying rate is greater than 60%, which is significantly higher than the effect of the compositions of the comparative examples. The compositions of the present invention can be used in the preparation of foods that promote defecation and reduce constipation.
[0132] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, rather than to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions of the technical solution of the present invention by ordinary technicians in this field do not deviate from the essence and scope of the technical solution of the present invention.
Claims
1. A composition for absorbing fat and promoting defecation, characterized in that: The composition comprises the following components in parts by weight: 5-30 parts of cassia seeds, 5-30 parts of sorbitol, 1-10 parts of monk fruit powder, 1-10 parts of aloe powder, 5-20 parts of orange peel powder, 5-30 parts of inulin and 5-30 parts of maltitol; Cassia seed composite particles are prepared by the following preparation method: 5-15% of the formulated amount of sorbitol is taken to prepare a sorbitol liquid with a mass concentration of 20%; the formulated amount of Cassia seeds is crushed, added into a fluidized bed granulator, mixed with the sorbitol liquid and fed into the fluidized bed granulator, and granulated under the conditions of an air inlet volume of 10-24 Hz, a slurry adding speed of 10-32 rpm, a material temperature of 39-46°C, an air outlet temperature of 30-52°C, and a pressure of 0.1-0.4 MPa to obtain the Cassia seed composite particles.
2. The composition according to claim 1, characterized in that The composition comprises the following components in parts by weight: 8-20 parts of cassia seeds, 8-20 parts of sorbitol, 3-8 parts of monk fruit powder, 3-8 parts of aloe powder, 10-15 parts of orange peel powder, 10-20 parts of inulin and 12-20 parts of maltitol.
3. The composition according to claim 1, characterized in that The composition comprises the following components in parts by weight: 15 parts of cassia seeds, 15 parts of sorbitol, 6 parts of monk fruit powder, 6 parts of aloe powder, 12 parts of orange peel powder, 15 parts of inulin and 16 parts of maltitol.
4. The composition according to any one of claims 1 to 3, characterized in that The particle size of the orange peel powder is 50-80 meshes, and the particle size of the inulin is 50-80 meshes.
5. The composition according to claim 4, characterized in that The particle size of the orange peel powder is 60 mesh, and the particle size of the inulin is 60 mesh.
6. The composition according to any one of claims 1 to 3, characterized in that Also included are flavoring agents; the flavoring agents are essences and salts.
7. A method for preparing the composition according to any one of claims 1 to 5, characterized in that: Includes steps: (1) granulating a formula amount of Cassia seed and 5-15% of a formula amount of sorbitol to obtain Cassia seed composite particles; then mixing the Cassia seed composite particles with a formula amount of aloe powder to obtain a mixture A; (2) mixing a formulated amount of monk fruit powder and a formulated amount of inulin to obtain a mixture B; (3) mixing the formulated amount of orange peel powder with the remaining formulated amount of sorbitol, granulating the mixture, and sieving the mixture to obtain a mixture C; (4) mixing mixture A and a formulated amount of maltitol to obtain mixture 1; (5) Mixing mixture B and mixture C to obtain mixture 2; (6) Mixture 1 and mixture 2 are mixed to obtain a composite material.
8. The preparation method according to claim 7, characterized in that: In step (1), the mixing is specifically: stirring at a speed of 5-20 rpm for 15-25 min; In step (2), the mixing is specifically: stirring at a speed of 5-20 rpm for 15-30 min; Step (3) is specifically as follows: the formulated amount of orange peel powder and the remaining formulated amount of sorbitol are stirred at a speed of 5-20 rpm for 5-15 minutes to obtain a mixed powder, which is placed in a granulator with a feeding speed of 3-30 rpm, a roller speed of 2-15 rpm, a roller pressure of 10-60 bar, a roller gap of 2-6 mm, a primary granulation screen of 10-20 mesh, a secondary finishing screen of 20-60 mesh, and a granulation cutter speed of 20-100 rpm to obtain a mixture C; In step (4) and step (5), the mixing is specifically: stirring at a speed of 5-20 rpm for 10-25 min; In step (6), the mixing is specifically: stirring at a rotation speed of 5-20 rpm for 15-30 min.
9. Use of the composition according to any one of claims 1 to 6 in preparing food having the functions of absorbing fat, promoting defecation and promoting gastrointestinal motility.
Citation Information
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