Enzyme immunoassay detector and enzyme immunoassay method

Abstract

The present invention is applicable to the field of detectors, and provides an enzyme immunoassay detector and an enzyme immunoassay method, the enzyme immunoassay detector comprises: a sample scanning device for scanning a to-be-detected sample to obtain sample information containing the sample number; a sampling needle is mounted on the sample adding device; the cup splicing device is used for splicing the reaction cups; the controller is in signal connection with the sample scanning device, the sample adding device and the cup splicing device and is used for controlling the cup splicing device to splice the reaction cups matched with the samples in number onto at least one to-be-detected plate; and controlling the sample adding device to perform sample adding on the reaction cups on the to-be-detected plate according to the sample information. The enzyme immunoassay detector can meet the detection requirements of different sample sizes, waste of reaction cups and coating reagents is avoided, cup splicing and sample filling can be automatically carried out according to scanned sample information, manual participation is not needed, and the enzyme immunoassay efficiency can be improved while the enzyme immunoassay cost is reduced.

Description

Technical Field

[0001] The invention belongs to the technical field of medical devices, and in particular relates to an enzyme immunoassay detector and an enzyme immunoassay method. Background Art

[0002] ELISA is an instrument that uses ELISA to perform sample immunoassay. ELISA combines the specificity of antigen-antibody reaction with the efficient catalytic effect of enzyme on substrate. The test result is determined by the color change after the enzyme acts on the substrate. ELISA plate is used as the carrier of the sample in ELISA and participates in the test together with the sample.

[0003] As an important carrier of ELISA, ELISA plate, in addition to being a reaction vessel, also coats antigen / antibody in its reaction micropores and participates in the test as a part of the reagent. Existing ELISA plates are usually whole plate type or strip type, and are provided with a number of interconnected reaction cups. When participating in the reagent detection with the sample, only the whole plate or the whole strip can be used, and the existing ELISA detector is also designed for the whole plate or the whole strip of ELISA plate. When the sample is detected, the reagent will be added to the whole row or the whole piece of reaction cups at the same time. However, when the amount of samples sent for inspection is limited, it takes a long time to collect samples, but if a small amount of samples is directly tested, the reaction cups that are not filled with samples are also filled with reagents, resulting in the waste of reaction cups that are not filled with samples and the reagents. Summary of the invention

[0004] The embodiment of the present invention provides an ELISA tester, which aims to solve the problem in the prior art that the ELISA tester may cause a large amount of waste of reaction cups and reagents and the ELISA test cost is high.

[0005] The embodiment of the present invention is implemented as follows:

[0006] An enzyme immunoassay detector, comprising:

[0007] A sample scanning device, used for scanning the sample to be tested to obtain sample information including the sample quantity;

[0008] A sample adding device, wherein a sampling needle is installed on the sample adding device;

[0009] A cup assembling device, which is used to assemble reaction cups;

[0010] The controller is respectively connected to the sample scanning device, the sample adding device and the cup assembling device by signals, and is used to control the cup assembling device to assemble reaction cups adapted to the number of samples onto at least one board to be tested, and to control the sample adding device to add samples to the reaction cups on the board to be tested according to the sample information.

[0011] Optionally, the enzyme immunoassay detector further comprises:

[0012] A plate carrier, used for placing the test plate, wherein the test plate is provided with a plurality of cup positions for placing independent reaction cups;

[0013] The controller is also used to control the sample adding device to complete the sample adding to the reaction cups on the board to be tested at the board loading position after the cup assembling device assembles the reaction cups matching the number of samples on at least one board to be tested.

[0014] Optionally, the sample information also includes a test item corresponding to the sample to be tested;

[0015] The controller is also used to determine the number of reaction cups required for each detection item according to the number of samples and the detection items corresponding to each of the samples to be detected; and control the cup assembly device to assemble the reaction cups onto at least one plate to be tested according to the number of reaction cups for each detection item, so that the plate to be tested is placed with reaction cups corresponding to different detection items.

[0016] Optionally, the cup-mixing device comprises:

[0017] The plate assembly and transfer mechanism is used to transfer the ELISA plate to the cup removal position, where a plurality of independent reaction cups are placed on the ELISA plate;

[0018] A cup transfer mechanism, used for transferring the independent reaction cup on the ELISA plate located at the cup removal position to the cup position;

[0019] The plate-splitting and plate-moving mechanism and the cup-moving mechanism are respectively connected and communicated with the controller, and the controller is used to control the plate-splitting and plate-moving mechanism and the cup-moving mechanism according to the sample information obtained by the sample scanning device to transfer the required reaction cup from the ELISA plate to the plate to be tested.

[0020] Optionally, the sample information also includes a test item corresponding to the sample to be tested; the ELISA tester also includes:

[0021] A plate storage device, used for storing ELISA plates corresponding to different detection items;

[0022] The controller is also used to determine the target position of the ELISA plate to be transferred in the plate storage device from the corresponding relationship between the ELISA plate items and the positions stored in advance according to the test items corresponding to the sample to be tested, and control the plate assembly and plate transfer mechanism to move to the target position to transfer the ELISA plate at the target position to the cup removal position.

[0023] Optionally, the controller is also used to control the cup transfer mechanism to assemble the reaction cups onto at least one plate to be tested according to the number of reaction cups required for the detection project each time the ELISA plate is transferred to the cup removal position, and then control the plate assembly and transfer mechanism to transfer the ELISA plate from the cup removal position back to the target position.

[0024] Optionally, the enzyme immunoassay detector further comprises:

[0025] An incubation device, used for incubating the reagents in the reaction cups assembled on the test plate and the filled samples to be tested, so as to obtain the liquid to be tested;

[0026] A detection device, used to detect the liquid to be tested in the reaction cup after incubation to obtain the detection result of the sample to be tested; and

[0027] A plate moving device is used to move the plate to be tested between the plate loading position, the incubation device and the detection device;

[0028] The scheduling and moving plate device is connected and communicated with the controller;

[0029] The controller is used to control the scheduling and moving device to move the board to be inspected.

[0030] Optionally, the scheduling and moving plate device includes a clamping plate mechanism, a second telescopic mechanism, and a second translation mechanism that drives the second telescopic mechanism to move;

[0031] The clamping mechanism includes a clamping jaw that can be opened and closed;

[0032] One end of the second telescopic mechanism is connected to the clamping mechanism, and the other end is connected to the second translation mechanism;

[0033] The second translation mechanism, the second telescopic mechanism and the clamping mechanism are respectively connected to and communicated with the controller;

[0034] The controller is used to control the second translation mechanism to move to a predetermined position, and then control the second telescopic mechanism to extend, thereby driving the clamping mechanism to complete the movement of the board to be inspected.

[0035] Optionally, the enzyme immunoassay detector further comprises:

[0036] A needle washing device, connected to the controller, for washing the sampling needle;

[0037] The controller is used to control the sample adding device to complete needle washing in the needle washing device.

[0038] The present invention also provides an ELISA method for use with the above-mentioned ELISA instrument, the method comprising:

[0039] Scanning the sample to be tested to obtain sample information including the number of samples;

[0040] Assembling reaction cups matching the number of samples onto at least one plate to be tested;

[0041] The sample is added to the reaction cup to be tested according to the sample information.

[0042] The ELISA detector provided by the present invention is provided with a sample scanning device for scanning the number of samples, a cup assembling device for assembling reaction cups, a sample adding device for adding or sampling samples, and a controller for controlling the cup assembling device, the sample adding device and the sample scanning device to operate autonomously. The cup assembling device can be controlled to automatically assemble reaction cups matching the number of samples onto a test board, and the sample adding device can be controlled to add samples or reagents to the reaction cups on the test board, so as to perform subsequent incubation and detection. The ELISA detector can meet the detection requirements of different sample amounts, avoid wasting reaction cups and coating reagents, and can automatically assemble cups and add samples according to the scanned sample information without manual intervention, thereby reducing the cost of ELISA and improving the efficiency of ELISA. BRIEF DESCRIPTION OF THE DRAWINGS

[0043] Figure 1 It is a structural schematic diagram of an ELISA tester provided in an embodiment of the present invention;

[0044] Figure 2 is a schematic structural diagram of a sample adding device provided in an embodiment of the present invention;

[0045] Figure 3 It is a structural schematic diagram of a carrier plate provided in an embodiment of the present invention;

[0046] Figure 4 is a schematic diagram of the structure of a cup-mixing device provided by an embodiment of the present invention;

[0047] Figure 5 is a structural schematic diagram of a scheduling and shifting plate device provided in an embodiment of the present invention;

[0048] Figure 6 It is a control connection block diagram of the controller of the present invention.

[0049] Figure Number:

[0050] 100, ELISA instrument; 110, reaction cup; 120, ELISA plate; 130, cup removal position; 140, incubation device; 150, detection device;

[0051] 200. Sample scanning device;

[0052] 300, sample adding device; 310, sampling needle; 320, first telescopic mechanism; 330, first translation mechanism;

[0053] 400, controller;

[0054] 500, board loading position; 510, board to be inspected;

[0055] 600, cup assembly device; 611, cup position; 620, assembly board and plate shifting mechanism; 630, cup shifting mechanism;

[0056] 700, the plate shifting device; 710, the plate clamping mechanism; 711, the clamping claw; 720, the second telescopic mechanism; 730, the second translation mechanism;

[0057] 800, needle washing device;

[0058] 900. Reagent box; 910. Refrigeration device. DETAILED DESCRIPTION

[0059] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0060] The ELISA detector provided by the present invention is provided with a sample scanning device for scanning the number of samples, a cup assembling device for assembling reaction cups, a sample adding device for adding or sampling samples, and a controller for controlling the cup assembling device, the sample adding device and the sample scanning device to operate autonomously. The cup assembling device can be controlled to automatically assemble reaction cups matching the number of samples onto a test plate, and the sample adding device can be controlled to add samples or reagents to the reaction cups on the test plate, so as to perform subsequent incubation and detection. The ELISA detector can meet the detection requirements of different sample amounts, avoid wasting reaction cups and coating reagents, and can automatically assemble cups and add samples according to the scanned sample information without manual intervention, thereby reducing the cost of ELISA and improving the efficiency of ELISA.

[0061] Example

[0062] refer to Figure 1 This embodiment provides an ELISA tester 100, comprising:

[0063] A sample scanning device 200 for detecting the number of samples;

[0064] The sample adding device 300 has a sampling needle 310 installed on it;

[0065] A cup assembling device 600, which is used to assemble reaction cups;

[0066] The controller 400 is signal-connected to the sample scanning device 200, the sample adding device 300 and the cup assembling device, respectively, and controls the cup assembling device 600 to assemble the reaction cups 110 adapted to the number of samples onto at least one plate to be tested 510, and controls the sample adding device 300 to add samples to the reaction cups 110 on the plate to be tested 510 according to the sample information.

[0067] In this embodiment, the ELISA instrument 100 scans the samples, and performs cup assembly and reagent filling according to the scanned sample quantity information.

[0068] The ELISA tester 100 includes a sample scanning device 200, a sample adding device 300 and a cup assembly device 600. The sample scanning device 200 can be a barcode or QR code scanning head, or a camera for image acquisition. The number of samples in the ELISA plate 120 can be scanned by the scanning head or the camera, and the scanned sample number information can be transmitted to the controller 400. The controller 400 can configure the type and amount of the reagent to be added according to the sample information transmitted by the sample scanning device 200.

[0069] The sample adding device 300 is equipped with a sampling needle 310, through which each reaction cup 110 is filled respectively. Different detection reagents are dripped into different reagents coated in the reaction cup 110, and multiple different tests are performed on the same ELISA plate at the same time to avoid waste of reagents or reaction cups.

[0070] It can be understood that the test plate contains a predetermined number of cup positions. If the number of reaction cups matching the number of samples is less than or equal to the predetermined number, they are assembled into one test plate. If the number of reaction cups matching the number of samples is greater than the predetermined number, the number of test plates required is greater than 1.

[0071] Specifically, the number of sampling needles 310 provided may be one, and each reaction cup 110 may be filled with reagents. A plurality of sampling needles 310 may also be provided as a group for reagent filling, which has a higher filling efficiency.

[0072] The sampling needle 310 can be connected to a structure or component for adding samples or taking samples, which can be a syringe with suction and extrusion functions. The sampling device 300 is also connected and communicated with the controller 400. Under the control of the controller 400, the sampling device 300 can move the sampling needle 310 to the cup mouth of any reaction cup 110, adjust the posture for alignment, and then perform actions such as sample filling or reagent filling according to the required amount.

[0073] The sample adding device 300 can add samples after the reaction cups are assembled on the test plate or during the assembly process. According to the test items contained in the scanning information of the sample to be tested, the sample to be tested is added to the corresponding reaction cup, and the corresponding reaction cup is coated with a primer corresponding to the test item.

[0074] The cup assembly device 600 is used to assemble the reaction cups 110. The reaction cups 110 are arranged on the ELISA plate 120. Different reagents need to be added according to the different primers in the reaction cups 110 for different reactions. The reaction cups 110 on a group of ELISA plates 120 are all filled with the same primer. The cup assembly device 600 is used to assemble the reaction cups 110 on different ELISA plates 120 onto a test plate 510, and the test plate 510 with assembled reaction cups 110 is used for reaction.

[0075] The cup-joining device joins cups according to the scanned sample information. After completing the cup-joining of one detection item, the sample required for the detection item is added to the reaction cup, and the cup-joining of the next detection item is carried out at the same time, so as to improve the efficiency of cup-joining and filling.

[0076] After all the cups are assembled, a sample to be tested can be sucked through the sampling needle and the sample to be tested can be added to the reaction cups of the test items to be tested. This method can reduce the number of times the sampling needle is cleaned, that is, the number of times the sampling needle is cleaned corresponds to the number of samples to be tested.

[0077] The controller 400 is connected and communicated with the sample scanning device 200, the sample adding device 300 and the cup assembling device 600. The controller 400 receives the number of scanned samples transmitted by the sample scanning device 200, and controls the cup assembling device 600 to assemble the reaction cup 110 according to the scanned sample number information, and calculates and generates control instructions such as the path and posture change steps of the sampling needle 310, and then controls the sampling needle 310 of the sample adding device 300 to add samples / reagents to the reaction cup 110 according to the generated control instructions. Among them, the controller 400 can be specifically one or more of a single chip microcomputer, a microcontroller, a combinational logic controller or a microprogram controller, or a computer with storage and computing capabilities.

[0078] The enzyme immunoassay tester 100 provided by the present invention scans the samples to be tested through the sample scanning device 200, converts the number of samples to be tested into computer-readable data, and transmits the readable data to the controller 400. After the readable data of the number of samples to be tested is integrated, analyzed and processed by the controller 400, a control instruction is generated, and the control instruction is sent to the cup-mixing device 600 and the sample adding device 300.

[0079] After receiving the control instruction, the cup assembly device 600 automatically assembles the reaction cups 110 of different test items and quantities required onto an ELISA plate 120. After receiving the control instruction from the controller 400, the sample adding device 300 drives the sampling needle 310 to move to the preset position of the target reaction cup 110, and controls the sampling needle 310 to add samples or reagents to the target reaction cup 110.

[0080] It should be noted that the target cuvette 110 is a cuvette 110 that needs to be filled with a sample or a reagent.

[0081] Since a sample scanning device 200 for scanning the number of samples, a cup-assembling device 600 for assembling reaction cups, a sample adding device 300 for adding or sampling samples, and a controller 400 for controlling the cup-assembling device 600, the sample adding device 300 and the sample scanning device 200 to operate autonomously are provided, the cup-assembling device 600 can be controlled to automatically assemble reaction cups 110 matching the number of samples onto the plate to be tested, and the sample adding device 300 can be controlled to add samples or reagents to the reaction cups 110 on the plate to be tested, so as to perform subsequent incubation and detection. The ELISA tester can meet the detection requirements of different sample quantities, avoid wasting reaction cups 110 and coating reagents, and can automatically assemble cups and add samples according to the scanned sample information without manual intervention, thereby reducing the cost of ELISA and improving the efficiency of ELISA.

[0082] Specifically, refer to Figure 2 The sample loading device 300 further includes a first telescopic mechanism 320 and a first translation mechanism 330 that drives the first telescopic mechanism 320 to move;

[0083] One end of the first telescopic mechanism 320 is connected to the sampling needle 310 , and the other end is connected to the first translation mechanism 330 ;

[0084] The first translation mechanism 330 and the first telescopic mechanism 320 are respectively connected to and communicated with the controller 400;

[0085] The controller 400 is used to control the first translation mechanism 330 to move to a predetermined position, and then control the first telescopic mechanism 320 to extend, thereby driving the sampling needle 310 to complete sampling or sample addition.

[0086] In this embodiment, the sample loading device 300 specifically includes a first telescopic mechanism 320 and a first translation mechanism 330 that drives the first telescopic mechanism 320 to move, wherein one end of the first telescopic mechanism 320 is connected to the sampling needle 310, and the other end is connected to the first translation mechanism 330. Through the first telescopic mechanism 320 and the first translation mechanism 330, the sampling needle 310 can move freely in the plane where the first telescopic mechanism 320 is located.

[0087] In one embodiment, the first telescopic mechanism 320 can be telescopically extended by a telescopic cylinder. The first telescopic mechanism 320 can drive the sampling needle 310 to move up and down in a vertical direction of its plane. The sampling needle 310 can be fixed to the lower end of the first telescopic mechanism 320 by inlaying, and the other end of the first telescopic mechanism 320 can be fixedly connected to the first translation mechanism 330 by means of a slider.

[0088] The first translation mechanism 330 is used to translate the first telescopic mechanism 320 and the sampling needle 310, which can specifically be a slide rail. At the same time, the first translation mechanism 330 can be fixed on the support surface through a gantry, and the first telescopic mechanism 320 and the first translation mechanism 330 can be fixed by a sliding connection. The first telescopic mechanism 320 can slide freely in the horizontal direction on the slide rail of the first translation mechanism 330. The first translation mechanism 330 and the first telescopic mechanism 320 can be communicated with the controller 400 through a data cable. The controller 400 can control the first translation mechanism 330 to reach a predetermined position in the horizontal direction, and then control the first telescopic mechanism 320 to extend, so that the sampling needle 310 reaches the sampling or sample addition position of the reaction cup 110, and the sampling or sample addition of the reaction cup 110 is completed through the sampling needle 310.

[0089] Based on the above structure, when it is necessary to add samples to the reaction cup 110 through the sampling needle 310, the controller 400 can send a translation instruction to the first translation mechanism 330. After receiving the translation instruction, the first translation device drives the first telescopic mechanism 320 and the sampling needle 310 to move in the horizontal direction. After reaching the predetermined position specified by the controller 400, the controller 400 can control the first telescopic mechanism 320 to extend, and the first telescopic mechanism 320 drives the sampling needle 310 to reach above the reaction cup 110, and injects reagents into the reaction cup 110 through the sampling needle 310 to complete the addition of samples; when sampling is required, the controller 400 is used to control the first horizontal mechanism to drive the first telescopic mechanism 320 to reach above the reaction cup 110, and the first telescopic mechanism 320 extends, and the sampling needle 310 extracts the reagent from the reaction cup 110 to complete the sampling of the reaction cup 110.

[0090] In summary, by providing a first translation mechanism 330, the first telescopic mechanism 320 and the sampling needle 310 can be driven to reach a predetermined position in the horizontal direction, and then the first telescopic mechanism 320 is driven by the controller 400 to drive the sampling needle 310 to move in the vertical direction, so that the sampling needle 310 can autonomously sample or add samples to the reaction cup 110, so that the integration level of the enzyme immunoassay detector 100 is higher, and the reaction cup 110 can be automatically sampled or added samples through the sample adding device 300.

[0091] In some embodiments, reference Figure 1 and Figure 3 , the ELISA detector 100 further includes:

[0092] A plate carrier 500 for placing a test plate 510, on which a plurality of cup positions 611 for placing independent reaction cups 110 are arranged;

[0093] The controller is also used to control the sample adding device 300 to complete the sample adding to the reaction cups 110 on the test board 510 at the plate loading position 500 after the cup assembling device 600 assembles the reaction cups 110 adapted to the number of samples on at least one test board 510 .

[0094] In this embodiment, after the cup assembly device 600 assembles the cuvettes 110 adapted to the number of samples onto at least one to-be-tested plate 510, the controller 400 is used to control the sample loading device 300 to load samples on all the cuvettes 110 located at the plate loading position 500. When the controller 400 is used to control the sample loading device 300 to load samples, the sample loading device 300 can load samples in sequence according to the sample information scanned by the sample scanning device 200. In this way, the loading of samples can be completed when the cuvette 110 is at the plate loading position 500, and time for checking the cuvette 110 is reserved, so as to facilitate timely discovery of the cuvette 110 that has been missed, and make the workflow of the ELISA tester 100 more reasonable.

[0095] In some embodiments, the sample information scanned by the sample scanning device 200 also includes the detection items corresponding to the sample to be detected;

[0096] The controller is also used to determine the number of reaction cups required for each detection item according to the number of samples and the detection items corresponding to each sample to be detected; and control the cup assembly device to assemble the reaction cups onto at least one board to be tested according to the number of reaction cups for each detection item, so that the board to be tested is placed with reaction cups corresponding to different detection items.

[0097] Specifically, the sample information scanned and obtained by the sample scanning device 200 may also include items to be tested for the sample to be tested. The same test sample may need to undergo multiple tests, or different test samples may need to undergo different test items. For different test items, a reaction cup 110 coated with corresponding reagents may be selected.

[0098] During the test, each sample needs to be tested for different items, and the sample scanning device 200 scans not only the number of samples, but also the test items corresponding to the samples to be tested. The sample information scanned by the sample scanning device 200 is transmitted to the controller 400, and the controller 400 determines the number of reaction cups 110 required for each test item according to the number of samples and the test items corresponding to each sample to be tested; and controls the cup assembly device to assemble the reaction cups onto at least one test plate 510 according to the number of reaction cups 110 for each test item, so that the test plate 510 is placed with reaction cups 110 corresponding to different test items.

[0099] When the cup-matching device 600 performs cup-matching, it will integrate the sample information obtained by the sample scanning device 200, sort out the number of the same test items, and generate instructions to control the cup-matching device 600 to take out the reaction cups 110 on the same ELISA plate, and take out the reaction cups 110 that match the number of test items corresponding to the ELISA plate. The frequency of moving in and out of the ELISA plates for each test item is reduced, and the efficiency of cup-matching is improved.

[0100] Specifically, cuvettes 110 corresponding to different test items are placed on the test plate 510 , and the cuvette assembly device 600 is used to assemble cuvettes 110 whose number matches the number of samples onto the test plate 510 .

[0101] The cup assembly device 600 is used to assemble the reaction cup 110 to the test board 510, and it may have the functions of clamping and transferring, and it may specifically be a mechanical arm with a clamping function. The reaction cups 110 with different functions are placed on the test board 510. Optionally, the cup assembly device 600 places the reaction cup 110 on the test board 510 according to the sample information scanned by the sample scanning device 200.

[0102] Optionally, the reaction cups 110 can be placed on the test plate 510 in sequence to facilitate the sample or reagent addition by the sample adding device 300.

[0103] The order of placing the reaction cups 110 may be to place them in the adjacent empty positions of the reaction cups 110 with the same function in sequence. The reaction cups 110 may be assembled manually according to the distribution result of the reaction cups 110 generated by the controller 400, or the result may be transmitted to a special device for assembling the reaction cups 110 to automatically assemble the reaction cups 110.

[0104] In some embodiments, the board to be inspected 510 is placed on the board loading position 500;

[0105] A plurality of cup positions 611 for placing independent reaction cups 110 are arranged on the test plate 510 .

[0106] Specifically, the area for placing the test plate 510 in the ELISA tester 100 is the plate carrier 500, and the test plate 510 can be placed in the plate carrier 500, wherein the test plate 510 is an ELISA plate assembled with different reaction cups 110 according to the sample information scanned by the scanning device 200 and the specific detection requirements.

[0107] In this way, cuvettes 110 with different functions are placed on the test plate 510, which facilitates the centralized addition or sampling of samples in the cuvettes 110 and facilitates the transfer of the cuvettes 110. At the same time, by placing the cuvettes 110 in order according to the scan information of the samples to be tested by the scanning device 200 on the test plate 510, cuvettes 110 with the same function can be placed in the same area of ​​the ELISA plate, and different types of cuvettes 110 can be distinguished during testing, reducing the difficulty of distinguishing the cuvettes 110 and facilitating efficient collection of test values.

[0108] The plate to be tested 510 is placed on the carrier position 500, and each cup position 611 is for placing a reaction cup 110 separately. In order to facilitate the placement of the reaction cup 110, the shape of the cup position 611 can be set to be circular. When the reaction cup 110 that needs to be reacted is placed on the plate to be tested 510, the reaction cup 110 enters the state of waiting for inspection.

[0109] When the reaction cup 110 is transferred, the controller 400 is used to control the cup assembly device 600 to grab the reaction cup 110. The cup assembly device 600 drives the reaction cup 110 to the carrier position 500, and places the reaction cup 110 in the cup position 611 on the test plate 510 in sequence until the number of reaction cups 110 and the corresponding items are the same as the number and corresponding items required for sample detection, and the transfer of the reaction cup 110 is completed. At this time, the test plate 510 with the assembled reaction cups 110 becomes the test plate 510.

[0110] By providing a cup position 611 on the test plate 510, the reaction cup 110 can be embedded in the cup position 611, and the reaction cup 110 can be stored separately in the independent cup positions 611. When the reaction cups 110 are transferred, collisions between the reaction cups 110 are avoided, which facilitates the centralized transfer of the reaction cups 110 and ensures the safety of the reaction cups 110 during transfer.

[0111] In some embodiments, reference Figure 4 The cup-combining device 600 includes:

[0112] The plate assembly and transfer mechanism 620 transfers the ELISA plate 120 to the cup removal position 130, wherein a plurality of independent reaction cups 110 are placed on the ELISA plate 120;

[0113] Transferring the independent reaction cup 110 on the ELISA plate 120 located at the cup removal position 130 to the cup transfer mechanism 630 on the cup position 611;

[0114] The plate-splicing and plate-moving mechanism 620 and the cup-moving mechanism 630 are respectively connected to and communicated with the controller 400 . The controller 400 is used to control the plate-splicing and plate-moving mechanism 620 and the cup-moving mechanism 630 according to the sample information obtained by the scanning device 200 , and to transfer the required reaction cup 110 from the ELISA plate 120 to the test plate 510 .

[0115] The ELISA plate 120 is provided with a reaction cup 110 containing a primer. The reaction cups 110 on the ELISA plates 120 with different functions contain different primers. Different reaction cups 110 need to be selected according to different detection requirements.

[0116] In this embodiment, the cup assembly device 600 includes: a plate assembly and plate moving mechanism 620 and a cup moving mechanism 630, wherein the plate assembly and plate moving mechanism 620 and the cup moving mechanism 630 are respectively connected to and communicated with the controller 400, and the plate assembly and plate moving mechanism 620 can transfer the ELISA plate 120 to the cup removal position 130 through the control of the controller 400, and the cup moving mechanism 630 can transfer the reaction cup 110 located at the cup removal position 130 from the ELISA plate 120 to the cup position 611 under the control of the controller 400.

[0117] Furthermore, the plate-jointing and plate-shifting mechanism 620 is used to translate the ELISA plate 120, which can be a conveyor belt with a conveying function. The ELISA plate 120 is placed on the plate-jointing and plate-shifting mechanism 620, and the ELISA plate 120 is conveyed to the cup-off position 130 by the plate-jointing and plate-shifting mechanism 620; the cup-shifting mechanism 630 is used to transfer the reaction cup 110, which can be a manipulator with a gripping function, and the reaction cup 110 on the ELISA plate 120 can be transferred to the cup position 611 through the control of the controller 400. The plate-jointing and plate-shifting mechanism 620 and the cup-shifting mechanism 630 are also communicatively connected to the controller 400, and the controller 400 can control the plate-jointing and plate-shifting mechanism 620 to continuously convey the ELISA plate 120 to the cup-off position 130, and at the same time, the cup-shifting mechanism 630 is controlled to continuously transfer the reaction cup 110 on the ELISA plate 120 to the cup position 611.

[0118] In this embodiment, when the reaction cup 110 needs to be transferred from the ELISA plate 120 to the cup position 611, the controller 400 is used to control the plate assembly and shifting mechanism 620 to translate the ELISA plate 120 to the cup removal position 130, and at the same time control the cup shifting mechanism 630 to rotate to the cup removal position 130, control the cup removal mechanism to grab the reaction cup 110, rotate the cup removal mechanism, and place the grabbed reaction cup 110 in the cup position 611, thereby completing the transfer of the reaction cup 110 from the ELISA plate 120 to the cup position 611.

[0119] Specifically, a detection device may be provided on the cup moving mechanism 630 . The detection device may be an infrared sensor, a radar detector, an ultrasonic detector, etc., and is connected to communicate with the controller 400 .

[0120] The detection device can transmit the position data of the reaction cup 110 on the ELISA plate 120 and the position data of the cup position 611 on the test plate 510 to the controller 400, and the controller 400 obtains the information of the reaction cup 110 on the ELISA plate 120 and the test plate 510 in real time.

[0121] When the cup moving mechanism 630 picks up the reaction cup 110 from the ELISA plate 120, the detection device scans the entire ELISA plate 120 and transmits the scanned data of the reaction cup 110 to the controller 400. The controller 400 controls the cup moving mechanism 630 to accurately reach above the reaction cup 110 and pick up the reaction cup 110. After that, when the cup moving mechanism 630 drives the reaction cup 110 to the test plate 510, the detection device scans the position of the cup position 611 on the test plate 510 and transmits the data information of the cup position 611 to the controller 400. The controller 400 controls the cup moving mechanism 630 to accurately place the reaction cup 110 on the cup position 611.

[0122] Optionally, in one embodiment, the automatic cup-assembling device can determine whether there is a reaction cup on the cup-taking position of the ELISA plate by using a detection signal received by the detection device at a cup-taking position before taking the cup, so as to control the cup-taking component to take the corresponding reaction cup from the cup-taking position when it is determined that there is a reaction cup on the cup-taking position; the automatic cup-assembling device can also determine whether a cup position on the empty plate is an empty cup position by using a detection signal received by the detection device at the cup position, so as to control the cup-taking component to move down to the cup position and retract the supporting portion of the cup-taking component to place the reaction cup in the cup position when it is determined that the cup position is an empty cup position.

[0123] In another embodiment, the detection device can also be arranged at a separate detection position, and the cup taking member moves to the detection position after taking the cup, and the detection position detects whether there is a cup.

[0124] The detection device can monitor the position of the reaction cup 110 on the ELISA plate 120 and the position of the reaction cup 110 on the test plate 510, so that the cup transfer mechanism 630 can pick up the reaction cup 110 on the ELISA plate 120, and also can facilitate the cup transfer mechanism 630 to place the reaction cup 110 on the cup position 611 of the test plate 510, so that the transfer of the reaction cup 110 is more accurate and the safety of the transfer of the reaction cup 110 is ensured.

[0125] In summary, the controller 400 is connected to the plate assembly and plate shifting mechanism 620 and the cup shifting mechanism 630 respectively, and communicates with both in real time, controls the plate assembly and plate shifting mechanism 620 to continuously shift the ELISA plate 120 to the cup removal position 130, and controls the cup shifting mechanism 630 to transfer the reaction cup 110 from the cup removal position 130 to the cup position 611, so that the transfer of the entire reaction cup 110 can be operated autonomously, which is convenient for controlling the speed and quantity of the transfer of the reaction cup 110.

[0126] In some embodiments, the sample information also includes the test items corresponding to the sample to be tested; the ELISA tester also includes:

[0127] A plate storage device, used to store ELISA plates corresponding to different test items;

[0128] The controller is also used to determine the target position of the ELISA plate to be transferred in the plate storage device from the corresponding relationship between the ELISA plate items and the positions stored in advance according to the test items corresponding to the sample to be tested, and control the plate assembly and plate shifting mechanism to move to the target position to transfer the ELISA plate at the target position to the cup removal position.

[0129] The plate storage device is used to store ELISA plates 120 corresponding to different detection items, so as to facilitate the plate assembly and transfer mechanism 620 to take the reaction cups 110 corresponding to the required detection items.

[0130] The controller 400 pre-stores the corresponding positions of the ELISA plates 120 of different detection items. When the reaction cup 110 of a certain detection item needs to be taken, the controller controls the plate assembly and shifting mechanism to move to the position corresponding to the ELISA plate 120 of the detection item, transfers the ELISA plate 120 to the cup removal position, and takes the reaction cup 110.

[0131] In some embodiments, the controller is also used to control the plate assembly and cup transfer mechanism 600 to assemble the reaction cups onto at least one plate to be tested according to the number of reaction cups required for the detection project each time the ELISA plate is transferred to the cup removal position, and then control the plate assembly and cup transfer mechanism to transfer the ELISA plate from the cup removal position back to the target position.

[0132] When the cuvette 110 on the ELISA plate 120 moved to the cup removal position is completely taken out, the controller 400 controls the plate assembly cup transfer mechanism 600 to send the ELISA plate 120 back to the original placement position.

[0133] In some embodiments, the controller generates a corresponding number of moving steps according to the number of samples scanned by the sample scanning device, and increases the number of moving steps according to the number of cups taken each time.

[0134] The scanning device 200 scans the samples, and sends the items that need to be tested for different samples to the controller 400. The controller 400 generates a corresponding cup-taking path. A complete cup-taking path includes the cup-taking position on the ELISA plate 120 and the cup-placing position on the test plate 510. When the reaction cup 110 at the corresponding position of the ELISA plate 120 is taken away, the position is marked, and the next time the cup-taking position on the ELISA plate 120 is increased in the number of moving steps, the cup-taking position is cleared. Similarly, when the reaction cup 110 is placed on the empty position on the test plate 510, the position is marked, and the next time the cup-placing position of the reaction cup 110 is increased in the number of moving steps, the cup-placing position is cleared.

[0135] In some embodiments, reference Figure 1 The ELISA tester 100 provided in this embodiment further includes:

[0136] An incubation device 140 for incubating the test plate 510, the incubation device 140 incubates the reagents in the reaction cups assembled on the test plate and the added test samples to obtain the test liquid;

[0137] A detection device 150 for detecting the test plate 510, the detection device 150 detects the test liquid in the incubated reaction cup to obtain a test result of the test sample; and

[0138] A scheduling and moving device 700 for moving the plate to be tested 510 between the plate loading position 500, the incubation device 140 and the detection device 150;

[0139] The dispatching plate moving device 700 is connected and communicated with the controller 400;

[0140] The controller 400 is used to control the scheduling board moving device 700 to move the board to be inspected 510 .

[0141] In this embodiment, the ELISA test instrument 100 is further provided with an incubation device 140, a detection device 150 and a scheduling and plate moving device 700, wherein the incubation device 140 is located on one side of the plate loading position 500, and the detection device 150 is on the upper left side of the ELISA test instrument 100, and there are plates to be tested 510 in the incubation device 140 and the detection device 150. When the plate to be tested 510 needs to be transferred from the incubation device 140 to the detection device 150, the controller 400 is used to control the scheduling and plate moving device 700 to clamp the plate to be tested 510 and transfer the plate to be tested 510 to the detection device 150; when the plate to be tested 510 located at the plate loading position 500 is transferred to the incubation device 140, the controller 400 is used to control the scheduling and plate moving device 700 to clamp the plate to be tested 510 at the plate loading position 500, transfer the plate to be tested 510 and place it in the incubation device 140. Thus, it is easy to understand that when the board to be inspected 510 on the board carrier 500 is transferred to the detection device 150, it can be realized by scheduling the board moving device 700. Among them, scheduling the board moving device 700 to clamp the incubation device 140 or transferring the board to be inspected 510 on the board carrier 500 to the detection device 150 is based on the regulation of the controller 400, so as to realize the free clamping of the scheduling board moving device 700.

[0142] In this way, by providing a scheduling and moving plate device 700 between the plate carrier position 500, the incubation device 140 and the detection device 150, the scheduling and moving plate device 700 can be conveniently used to autonomously transfer the plate to be inspected 510 between the plate carrier position 500, the incubation device 140 and the detection device 150 under the control of the controller 400, without the need for human intervention, with a high degree of automation, thus saving detection time and shortening the detection cycle.

[0143] In some embodiments, reference Figure 5The plate shifting device 700 includes a clamping mechanism 710, a second telescopic mechanism 720, and a second translation mechanism 730 that drives the second telescopic mechanism 720 to move;

[0144] The clamping mechanism 710 includes a clamping jaw 711 that can be opened and closed;

[0145] One end of the second telescopic mechanism 720 is connected to the clamping mechanism 710 , and the other end is connected to the second translation mechanism 730 ;

[0146] The second translation mechanism 730, the second telescopic mechanism 720 and the clamping mechanism 710 are respectively connected to the controller 400 for communication;

[0147] The controller 400 is used to control the second translation mechanism 730 to move to a predetermined position, and then control the second telescopic mechanism 720 to extend, thereby driving the clamping mechanism 710 to complete the movement of the inspection plate 510 .

[0148] In this embodiment, the scheduling and moving plate device 700 specifically includes a second telescopic mechanism 720, a second translation mechanism 730 that drives the second telescopic mechanism 720 to move, and a clamping mechanism 710 with a clamping claw 711, wherein one end of the second telescopic mechanism 720 is connected to the clamping mechanism 710, and the other end is connected to the second translation mechanism 730. The second telescopic mechanism 720 and the second translation mechanism 730 can enable the clamping mechanism 710 to move freely in the plane where the second telescopic mechanism 720 is located. The clamping claw 711 serves as a clamping component for the plate to be inspected 510, and it can have an opening and closing function.

[0149] The second telescopic mechanism 720 can be telescoped by a telescopic cylinder, which can drive the clamping mechanism 710 to move up and down in its vertical direction. The clamping mechanism 710 can be fixed to one end of the second telescopic mechanism 720 by means of a sleeve inlay, and the other end of the second telescopic mechanism 720 can be fixedly connected to the second translation mechanism 730 by means of a slider; the second translation mechanism 730 is a component used to translate the second telescopic mechanism 720 and the clamping mechanism 710, which can specifically be a slide rail, and the second translation mechanism 730 can be fixed to the support surface by means of a gantry. The second telescopic mechanism 720 and the second translation mechanism 730 can be fixed by connecting with a slide rail. The second telescopic mechanism 720 can slide freely in the horizontal direction on the slide rail of the second translation mechanism 730. The second translation mechanism 730 and the second telescopic mechanism 720 are connected and communicated with the controller 400. The second translation mechanism 730 can be moved in the horizontal direction through the control of the controller 400, and then the second telescopic mechanism 720 can be controlled to extend, so that the clamping mechanism 710 clamps the board to be inspected 510, and transfers and places the board to be inspected 510 to the designated area.

[0150] When it is necessary to clamp the board to be inspected 510 through the clamping mechanism 710, the controller 400 can send a translation command to the second translation mechanism 730. After receiving the translation command, the second translation device drives the second telescopic mechanism 720 and the clamping mechanism 710 to move in the horizontal direction. After reaching the predetermined position specified by the controller 400, the controller 400 can control the second telescopic mechanism 720 to extend, and the second telescopic mechanism 720 drives the clamping mechanism 710 to reach one side of the board to be inspected 510. The clamping claw 711 of the clamping mechanism 710 will clamp the board to be inspected 510. Afterwards, the controller 400 is used to control the second telescopic mechanism 720 and the second translation mechanism 730 to bring the board to be inspected 510 to the incubation device 140, the plate carrier 500 or the detection device 150, and then release the clamping claw 711 to place the board to be inspected 510 in the corresponding area to complete the transfer of the board to be inspected 510.

[0151] In summary, the controller 400 is used to control the second translation mechanism 730, the second telescopic mechanism 720 and the clamping mechanism 710, so as to complete the automated transfer of the test plate 510, and the test plates 510 in different areas and with different incubation times can be transferred, thereby increasing work efficiency.

[0152] In some embodiments, reference Figure 1 The ELISA tester 100 provided in this embodiment further includes:

[0153] A needle washing device 800 for washing the sampling needle 310, the needle washing device 800 is connected to the controller 400 and is used for washing the sampling needle 310;

[0154] The controller 400 is used to control the sample adding device 300 to complete the needle washing in the needle washing device 800 .

[0155] In this embodiment, the ELISA tester 100 further includes a reagent kit 900, which is arranged on a side close to the plate carrier 500. After the sample loading device 300 completes the loading or sampling of the reaction cup 110 on the plate carrier 500, the sampling needle 310 can be cleaned by the needle washing device 800; wherein, there can be one or more needle washing devices 800, and the specific number can be adjusted according to the actual sample loading device 300. The needle washing device 800 is used to clean the sampling needle 310, which can be a common sampling needle cleaning device, which uses a cleaning liquid to clean the sampling needle 310 and automatically air-dries it to prevent the residual cleaning liquid from affecting the detection effect.

[0156] When the sample volume is large, the sampling needle 310 is usually replaced directly to avoid the time-consuming needle washing and reduce the detection efficiency. However, when the sample volume is small, the small number of samples leads to a small number of sampling or reagent addition times. The needle washing method will not delay too much time and has little impact on the detection efficiency. At the same time, it can reduce the consumption of the sampling needle 310 and save detection costs.

[0157] The controller 400 is used to control the sample loading device 300 to sample or load the reaction cup 110 placed on the carrier position 500. When the sample loading device 300 completes the loading or sampling of all the same type of reaction cups 110, or needs to load or sample reaction cups 110 of different types, the controller 400 is used to control the sample loading device 300 to run above the needle washing device 800, and insert the sampling needle 310 into the needle washing device 800, and the sampling needle 310 is cleaned by the needle washing device 800 to complete the needle washing.

[0158] By providing a needle washing device 800 on one side of the carrier position 500, the device layout is more reasonable. After completing the sample addition or sampling of the reaction cup 110, the sampling needle 310 can be quickly cleaned, saving the cleaning time of the sample addition device 300 and avoiding reagent contamination between different types of reaction cups 110.

[0159] In some embodiments, reference Figure 1 , the ELISA detector 100 further includes:

[0160] A reagent kit 900 is provided with a refrigeration device 910, and the reagent kit 900 is used to contain various types of reaction reagents;

[0161] The controller 400 is used to control the sampling needle 310 to extract the reagent corresponding to the reaction cup 110 to which the reagent currently needs to be added in the reagent ...

[0162] In this embodiment, the ELISA tester 100 is further provided with a reagent kit 900, which is arranged on one side of the detection device 150 and contains different reaction reagents. The reagent kit 900 has a refrigeration device 910, which can be a refrigeration tube with a refrigeration function or an ice maker with an ice making function. The reagent kit 900 can be embedded in the refrigeration device 910, and the refrigeration device 910 is used to reduce the temperature of the reagent kit 900.

[0163] The detection reagent needs to be stored at low temperature to avoid inactivation or failure of the reagent. In the traditional way, the detection reagent is injected from the reagent bottle into the reagent kit 900, and after the test is completed, the detection reagent is poured back into the reagent bottle for low temperature storage. The operation is cumbersome and it is easy to cause the reagent to remain in the reagent kit, resulting in reagent waste. The refrigeration device 910 is provided on the reagent kit 900, so that the reagent can be stored in the reagent kit 900 for a long time, avoiding the need to pour the detection reagent back and forth between the reagent bottle and the reagent kit 900.

[0164] The reaction cup 110 is filled with a primer. After adding the sample, different reagents need to be dripped into the reaction cups 110 with different primers for reaction. The controller 400 adds the sample to the reaction cups 110 with different primers according to the sample information scanned by the scanning device 200 and the detection requirements, and drips different reagents according to different reaction cups 110.

[0165] The reagent box 900 is used to store the samples after the test is completed. It can be a box body with a square shape and can be embedded in the refrigeration device 910. The number of refrigeration devices 910 can be set according to actual needs, and can be one or more. Each refrigeration device 910 is provided with a plurality of grooves for the reagent box 900 to be embedded. The reagent box 900 is installed in the refrigeration device 910 in sequence.

[0166] Based on the above structure, a refrigeration device 910 and a reagent kit 900 are provided on one side of the detection device 150, and the reagent kit 900 is placed in the refrigeration device 910, which is convenient for loading the sample on the detection device 150 into the reagent kit 900, and also convenient for storing the reagent kit 900 in the refrigeration device 910, making the transfer and preservation of the samples more reasonable.

[0167] The present invention also provides an ELISA method, which is applied to the above-mentioned ELISA instrument, and the method comprises:

[0168] Scanning the sample to be tested to obtain sample information including the number of samples;

[0169] Assembling reaction cups matching the number of samples onto at least one plate to be tested;

[0170] Fill the reaction cup to be tested with samples according to the sample information.

[0171] Scan the sample to be tested to obtain sample information, which includes the sample quantity. According to the obtained sample information, assemble the reaction cups adapted to the sample quantity onto at least one plate to be tested, and fill the reaction cups on the plate to be tested with samples according to the sample information.

[0172] When the sample volume is small, the reaction cups are assembled onto at least one test plate according to the number of samples, and the reaction cups containing the samples are filled with samples or reagents to avoid waste of reagents, save test consumables, and reduce test costs.

[0173] It is understandable that those skilled in the art can, under the guidance of the above embodiments, combine various implementation methods in the above embodiments to obtain technical solutions of multiple implementation methods.

[0174] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. An enzyme immunoassay detector, characterized in that: include: A sample scanning device, used for scanning the sample to be tested to obtain sample information including the sample quantity; A sample adding device, wherein a sampling needle is installed on the sample adding device; A cup assembling device, which is used to assemble reaction cups; The controller is respectively connected to the sample scanning device, the sample adding device and the cup assembling device by signals, and is used to control the cup assembling device to assemble reaction cups adapted to the number of samples onto at least one board to be tested, and to control the sample adding device to add samples to the reaction cups on the board to be tested according to the sample information.

2. The ELISA tester according to claim 1, characterized in that: The enzyme immunoassay detector also includes: A plate carrier, used for placing the plate to be tested, wherein the plate to be tested is provided with a plurality of cup positions for placing independent reaction cups; The controller is also used to control the sample adding device to complete the sample adding to the reaction cups on the board to be tested at the board loading position after the cup assembling device assembles the reaction cups matching the number of samples on at least one board to be tested.

3. The ELISA tester according to claim 1, characterized in that: The sample information also includes the test items corresponding to the sample to be tested; The controller is also used to determine the number of reaction cups required for each detection item according to the number of samples and the detection items corresponding to each of the samples to be detected; and control the cup assembly device to assemble the reaction cups onto at least one plate to be tested according to the number of reaction cups for each detection item, so that the plate to be tested is placed with reaction cups corresponding to different detection items.

4. The ELISA tester according to claim 2, characterized in that: The cup-combining device comprises: The plate assembly and transfer mechanism is used to transfer the ELISA plate to the cup removal position, where a plurality of independent reaction cups are placed on the ELISA plate; A cup transfer mechanism, used for transferring the independent reaction cup on the ELISA plate located at the cup removal position to the cup position; The plate-splitting and plate-moving mechanism and the cup-moving mechanism are respectively connected and communicated with the controller, and the controller is used to control the plate-splitting and plate-moving mechanism and the cup-moving mechanism according to the sample information obtained by the sample scanning device to transfer the required reaction cup from the ELISA plate to the plate to be tested.

5. The ELISA tester according to claim 4, characterized in that: The sample information also includes the test items corresponding to the sample to be tested; the ELISA tester also includes: A plate storage device, used for storing ELISA plates corresponding to different detection items; The controller is also used to determine the target position of the ELISA plate to be transferred in the plate storage device from the corresponding relationship between the ELISA plate items and the positions stored in advance according to the test items corresponding to the sample to be tested, and control the plate assembly and plate transfer mechanism to move to the target position to transfer the ELISA plate at the target position to the cup removal position.

6. The ELISA tester according to claim 5, characterized in that: The controller is also used to control the plate assembly and cup transfer mechanism to assemble the reaction cups onto at least one plate to be tested according to the number of reaction cups required by the detection project after the ELISA plate is transferred to the cup removal position each time, and then control the plate assembly and cup transfer mechanism to transfer the ELISA plate from the cup removal position back to the target position.

7. The ELISA tester according to claim 4, characterized in that: The enzyme immunoassay detector also includes: An incubation device, used for incubating the reagents in the reaction cups assembled on the test plate and the filled samples to be tested, so as to obtain the liquid to be tested; A detection device, used to detect the liquid to be tested in the reaction cup after incubation to obtain the detection result of the sample to be tested; and A plate-moving device is used to move the plate to be tested between the plate-carrying position, the incubation device and the detection device; The scheduling and moving plate device is connected and communicated with the controller; The controller is used to control the scheduling and moving device to move the board to be inspected.

8. The ELISA tester according to claim 7, characterized in that: The dispatching and shifting plate device comprises a clamping plate mechanism, a second telescopic mechanism and a second translation mechanism that drives the second telescopic mechanism to move; The clamping mechanism includes a clamping jaw that can be opened and closed; One end of the second telescopic mechanism is connected to the clamping mechanism, and the other end is connected to the second translation mechanism; The second translation mechanism, the second telescopic mechanism and the clamping mechanism are respectively connected to and communicated with the controller; The controller is used to control the second translation mechanism to move to a predetermined position, and then control the second telescopic mechanism to extend, thereby driving the clamping mechanism to complete the movement of the board to be inspected.

9. The ELISA tester according to claim 1, characterized in that: The enzyme immunoassay detector also includes: A needle washing device, connected to the controller, for washing the sampling needle; The controller is used to control the sample adding device to complete needle washing in the needle washing device.

10. An enzyme immunoassay method, characterized in that: Applied to the ELISA tester according to any one of claims 1 to 9, the method comprising: Scanning the sample to be tested to obtain sample information including the number of samples; Assembling reaction cups matching the number of samples onto at least one plate to be tested; The sample is added to the reaction cup to be tested according to the sample information.

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