Self-coupling repair method for deep stratum CO2 adsorption, storage and isolation failure

Abstract

The invention relates to the technical field of sealing technologies, in particular to a deep stratum CO2 adsorption, sealing and isolation failure self-coupling repairing method which comprises the following steps: S10, spraying a Li salt culture medium and Li-CO2 sensitive salt-tolerant anaerobic fungi at the position 5 meters thick on the outer vertical surface of an isolation layer of a CO2 adsorption layer to form an isolation layer technology implementation area; s20, an initial Li salt culture medium contains high-concentration insoluble Li2CO3, but the concentration of water-soluble LiHCO3 and CO2 in an isolation layer technology implementation area is too low, and the growth conditions of Li-CO2 sensitive salt-tolerant anaerobic fungi are not met; s30, when a CO2 adsorption layer is isolated to generate cracks, a large amount of adsorbed CO2 escapes, when the CO2 passes through a spraying layer, the escaped CO2 accelerates the hydrolysis of Li2CO3 into LiHCO3 and forms diffusion around the LiHCO3, the concentration of Li and the concentration of CO2 in a liquid-phase environment are increased at the same time, and elements such as N, P and K of a Li salt culture medium in a low-lithium salt state are supplemented; the method has the advantages of simplicity and convenience in operation, low cost, environmental protection and high efficiency, and solves the problem of isolation weakening or failure caused by pile aging or geological activity in the underground CO2 adsorption and storage process.

Description

Technical Field

[0001] The present invention relates to the technical field of storage technology, and in particular to a method for self-coupling repair of isolation failure of deep formation CO2 adsorption storage. Background Art

[0002] In the context of increasingly serious global warming and climate change, carbon dioxide (CO2) emission control and adsorption technology has received widespread attention. Although the traditional underground CO2 adsorption storage and isolation method has slowed down the release of CO2 to a certain extent, during long-term use, the adsorption layer may cause CO2 to escape due to cracks or structural failure, affecting the isolation effect.

[0003] There are currently a variety of physical and chemical methods for repairing cracks in the CO2 adsorption layer, but these methods have problems such as high cost, complex operation, and great environmental impact. In addition, the current methods require the intervention of a third party to achieve their functions. Therefore, finding an efficient, environmentally friendly, low-cost method that can actively identify and repair has become a technical problem that needs to be solved urgently.

[0004] In this context, a "self-coupling repair method for deep formation CO2 adsorption storage isolation failure" came into being. This method uses the self-coupling and growth characteristics of Li-CO2 sensitive salt-tolerant anaerobic fungi under specific environmental conditions, and controls the growth of Li-CO2 sensitive salt-tolerant anaerobic fungi by regulating the lithium salt (Li salt) content in the culture medium, thereby achieving the purpose of repairing cracks and blocking CO2 escape. Summary of the invention

[0005] The purpose of the present invention is to provide a deep formation CO2 adsorption storage isolation failure auto-coupling repair method in view of the defects and shortcomings of the prior art.

[0006] The present invention discloses a method for self-coupling repair of deep formation CO2 adsorption storage isolation failure, which adopts the following steps:

[0007] S10: Spraying Li salt culture medium and Li-CO2 sensitive salt-tolerant anaerobic fungi at a thickness of 5 meters on the outer surface of the CO2 adsorption layer isolation layer to form an isolation layer technology implementation area;

[0008] S20: The initial Li salt culture medium contains a high concentration of insoluble Li2CO3, but the concentrations of water-soluble LiHCO3 and CO2 in the isolation layer technology implementation area are too low to meet the growth conditions of Li-CO2 sensitive salt-tolerant anaerobic fungi, resulting in failure to grow;

[0009] S30: When cracks appear in the isolation of the CO2 adsorption layer, a large amount of adsorbed CO2 escapes. When passing through the spray layer, the escaped CO2 will accelerate the hydrolysis of Li2CO3 into LiHCO3 and diffuse around it. The Li concentration and CO2 concentration in the liquid environment increase at the same time. Supplemented by the N, P, K and other elements of the Li salt culture medium in the low lithium salt state, the growth conditions of Li-CO2 sensitive and salt-tolerant anaerobic fungi are met, and they grow rapidly to fill the crack space and gradually complete the crack plugging process. The crack plugging speed has the characteristics of "acceleration-constant speed-deceleration-inhibition waiting". During the waiting period, the Li-CO2 sensitive and salt-tolerant anaerobic fungi are in a semi-inhibited state, waiting for the arrival of the next high CO2 escape cycle.

[0010] Furthermore, the isolation layer technology implements active control characteristics of the zone environment:

[0011] Since the Li salt culture medium only contains insoluble Li2CO3, there is a lack of free CO2 around the well-isolated technical implementation area, and the hydrolysis of Li2CO3 cannot be completed, so that the isolation layer technical implementation area lacks water-soluble Li elements and C sources at the same time, and does not meet the basic growth nutritional conditions of Li-CO2 sensitive salt-tolerant anaerobic fungi;

[0012] The growth conditions of Li-CO2-sensitive salt-tolerant anaerobic fungi are met only when the LiHCO3 concentration is higher than the critical concentration of 5g / L and there is a continuous supply of CO2; thus, when the technical implementation area is well isolated, a patented method implementation area with waiting characteristics is formed at a 5-meter-thick position on the outer facade of the CO2 adsorption layer isolation layer, with isolation failure as the control condition.

[0013] Furthermore, the Li salt culture medium is a non-water-soluble culture medium with a key ingredient and has a CO2 coupled switch characteristic. The culture is basically evenly dispersed in the technical implementation area. When the isolation fails and CO2 escapes, Li2CO3 is hydrolyzed into water-soluble LiHCO3 in a humid environment, and diffuses to the surrounding area accompanied by surface water, thereby realizing the supplement of water-soluble Li elements in the technical implementation area. Furthermore, if the LiHCO3 in the technical implementation area decreases, Li2CO3 is hydrolyzed and supplemented in real time, so that the area is always kept in a nearly saturated LiHCO3 state, meeting the growth requirements of Li-CO2 sensitive and salt-tolerant anaerobic fungi, thereby entering an active state.

[0014] Furthermore, the Li-CO2 sensitive salt-tolerant anaerobic fungi in step S10 are obtained from a lithium-rich salt lake, specifically:

[0015] S1010: A Li-CO2 sensitive salt-tolerant anaerobic fungus screening medium is prepared based on the Li salt medium formula, and the LiHCO3 concentration is controlled to form three Li gradient mediums, wherein the LiHCO3 concentrations are set to 100% saturation concentration, half-saturation concentration, and 20% concentration, respectively, and made into a gel and applied to a culture dish;

[0016] S1020: After absorbing the brine sample from the surface of the lithium-rich salt lake sediment layer and shaking it thoroughly, the slag is removed and the concentrated bacterial solution is obtained by high-speed centrifugation. The concentrated solution is applied to the surface of the culture medium gel and placed in a CO2 incubator at 25°C for cultivation. The growth of Li-CO2-sensitive and salt-tolerant anaerobic fungi around the introduction point is observed microscopically, and a growth curve is drawn according to the change in the size of the bacterial plaque. Then, excellent mycelium is selected for further cultivation by the streaking method. After the isolated mycelium is obtained, the expansion is completed by the CO2 aeration method, and finally the strain required by this patent is obtained;

[0017] S1030: The Li-CO2-sensitive, salt-tolerant anaerobic fungi bred from lithium-rich salt lakes are salt-tolerant, low-temperature bacteria that meet the geological requirements for the construction of a CO2 adsorption layer near the constant temperature layer. The strain growth does not require external heating;

[0018] S1040: During the transition from nutritional inhibition to active growth, the growth of Li-CO2-sensitive salt-tolerant anaerobic fungi has the following characteristics: first, it undergoes a gradually accelerated growth process, and then gradually enters a balanced and stable growth process according to the growth curve and the actual supply rate of CO2. The crack plugging effect is significant, the CO2 supply decreases, the Li2CO3 hydrolysis effect weakens, and the Li and CO2 supply capacities decrease simultaneously. The growth rate of the fungus slows down due to nutritional restriction until the plugging is successful and CO2 escape is inhibited. The growth of Li-CO2-sensitive salt-tolerant anaerobic fungi enters a dormant state again due to nutritional inhibition, thus experiencing the process of "acceleration-constant speed-deceleration-inhibition waiting".

[0019] Furthermore, the auto-coupling and isolation repair is based on the escape of CO2 from the adsorption layer due to isolation failure as a basic condition, and the Li-CO2 dual conditions are completed by synchronously hydrolyzing Li2CO3 in the isolation layer technology implementation area with CO2; the auto-coupling and is the control of the repair process that is automatically completed by the implementation of this technology without changing the CO2 concentration in the isolation layer technology implementation area, and the growth control of Li-CO2 sensitive salt-tolerant anaerobic fungi is also based on the escape of CO2 as the basic control condition, and changes in the concentration of a single water-soluble LiHCO3 or CO2 cannot meet the nutritional needs of the growth of Li-CO2 sensitive salt-tolerant anaerobic fungi.

[0020] The beneficial effects of the present invention are as follows: the self-coupling repair method for deep formation CO2 adsorption storage isolation failure described in the present invention sprays a Li salt culture medium containing a high concentration of Li2CO3 and Li-CO2 sensitive salt-tolerant anaerobic fungi on the outer surface of the CO2 adsorption layer isolation layer. When cracks appear in the adsorption layer and cause CO2 to escape, the escaped CO2 reacts with Li2CO3 to generate LiHCO3, thereby forming a supplement of Li nutrient elements, and at the same time combines with the escaped CO2 to realize the supply of C elements, thereby allowing the Li-CO2 sensitive salt-tolerant anaerobic fungi to break away from the inhibition state, grow rapidly and fill the cracks, and gradually complete the plugging process;

[0021] During the CO2 storage process, with the aging of the pile or geological activities, the isolation will be weakened or even fail. This patent proposes a new isolation self-coupling and repair technology, and designs a switch-type culture medium. With the help of the low-temperature hydrolysis characteristics of Li2CO3 in CO2 atmosphere, when the isolation fails with the escape of CO2, the Li2CO3 in the Li salt culture medium is hydrolyzed under the action of CO2 to generate LiHCO3 and diffuses in water, providing Li nutrients for Li-CO2 sensitive salt-tolerant anaerobic fungi; CO2 provides a C source for the bacteria; with the help of N, P, K and other nutrient supplies in the Li salt culture medium, Li-CO2 sensitive salt-tolerant anaerobic fungi resume growth, gradually filling the cracks until the CO2 escape is lower than the basic growth requirements of Li-CO2 sensitive salt-tolerant anaerobic fungi, completing the isolation and repair; Li-CO2 sensitive salt-tolerant anaerobic fungi are inhibited by Li and C sources and re-enter dormancy. This technical method can use biological perception to complete isolation and repair in a timely manner without external intervention, and has strong autonomy and reliable performance.

[0022] This technology cleverly utilizes the control conditions of CO2 on the hydrolysis of Li2CO3 and the growth characteristics of Li-CO2 sensitive salt-tolerant anaerobic fungi under the conditions where Li and C are simultaneously present, to achieve active monitoring characteristics of isolation effectiveness. When isolation fails, it can simultaneously achieve growth control of Li-CO2 sensitive salt-tolerant anaerobic fungi according to the characteristics of crack development, and timely complete the purpose of repairing cracks and blocking CO2 escape. It has the advantages of simple operation, low cost, environmental protection and high efficiency, and effectively solves the problem of isolation weakening or failure caused by pile aging or geological activities during underground CO2 adsorption and storage. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] The drawings described herein are used to provide a further understanding of the present invention and constitute a part of the present application, but do not constitute an improper limitation of the present invention. In the drawings:

[0024] Figure 1 It is the technical roadmap of the present invention.

[0025] Figure 2This is the growth curve of the plaque size change of Li-CO2 sensitive salt-tolerant anaerobic fungi in the lithium gradient concentration cultivation experiment

[0026] Figure 3 It is a schematic diagram of the layer structure of the isolation layer composition and technical implementation layer layout. DETAILED DESCRIPTION

[0027] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments, wherein the illustrative embodiments and descriptions are only used to explain the present invention but are not intended to limit the present invention.

[0028] like Figure 1 As shown, the method for self-coupling repair of CO2 adsorption storage isolation failure in deep formations described in this specific embodiment adopts the following steps:

[0029] S10: Spraying Li salt culture medium and Li-CO2 sensitive salt-tolerant anaerobic fungi at a thickness of 5 meters on the outer surface of the CO2 adsorption layer isolation layer to form an isolation layer technology implementation area;

[0030] S20: The initial Li salt culture medium contains a high concentration of insoluble Li2CO3, but the concentrations of water-soluble LiHCO3 and CO2 in the isolation layer technology implementation area are too low to meet the growth conditions of Li-CO2 sensitive salt-tolerant anaerobic fungi, resulting in failure to grow;

[0031] S30: When cracks appear in the isolation of the CO2 adsorption layer, a large amount of adsorbed CO2 escapes. When passing through the spray layer, the escaped CO2 will accelerate the hydrolysis of Li2CO3 into LiHCO3 and diffuse around it. The Li concentration and CO2 concentration in the liquid environment increase at the same time. Supplemented by the N, P, K and other elements of the Li salt culture medium in the low lithium salt state, the growth conditions of Li-CO2 sensitive and salt-tolerant anaerobic fungi are met, and they grow rapidly to fill the crack space and gradually complete the crack plugging process. The crack plugging speed has the characteristics of "acceleration-constant speed-deceleration-inhibition waiting". During the waiting period, the Li-CO2 sensitive and salt-tolerant anaerobic fungi are in a semi-inhibited state, waiting for the arrival of the next high CO2 escape cycle.

[0032] Furthermore, the isolation layer technology implements active control characteristics of the zone environment:

[0033] Since the Li salt culture medium only contains insoluble Li2CO3, there is a lack of free CO2 around the well-isolated technical implementation area, and the hydrolysis of Li2CO3 cannot be completed, so that the isolation layer technical implementation area lacks water-soluble Li elements and C sources at the same time, and does not meet the basic growth nutritional conditions of Li-CO2 sensitive salt-tolerant anaerobic fungi;

[0034] The growth conditions of Li-CO2-sensitive salt-tolerant anaerobic fungi are met only when the LiHCO3 concentration is higher than the critical concentration of 5g / L and there is a continuous supply of CO2; thus, when the technical implementation area is well isolated, a patented method implementation area with waiting characteristics is formed at a 5-meter-thick position on the outer facade of the CO2 adsorption layer isolation layer, with isolation failure as the control condition.

[0035] Furthermore, the Li salt culture medium is a non-water-soluble culture medium with a key ingredient and has a CO2 coupled switch characteristic. The culture is basically evenly dispersed in the technical implementation area. When the isolation fails and CO2 escapes, Li2CO3 is hydrolyzed into water-soluble LiHCO3 in a humid environment, and diffuses to the surrounding area accompanied by surface water, thereby realizing the supplement of water-soluble Li elements in the technical implementation area. Furthermore, if the LiHCO3 in the technical implementation area decreases, Li2CO3 is hydrolyzed and supplemented in real time, so that the area is always kept in a nearly saturated LiHCO3 state, meeting the growth requirements of Li-CO2 sensitive and salt-tolerant anaerobic fungi, thereby entering an active state.

[0036] Furthermore, the Li-CO2 sensitive salt-tolerant anaerobic fungi in step S10 are obtained from a lithium-rich salt lake, specifically:

[0037] S1010: A Li-CO2 sensitive salt-tolerant anaerobic fungus screening medium is prepared based on the Li salt medium formula, and the LiHCO3 concentration is controlled to form three Li gradient mediums, wherein the LiHCO3 concentrations are set to 100% saturation concentration, half-saturation concentration, and 20% concentration, respectively, and made into a gel and applied to a culture dish;

[0038] S1020: After absorbing the brine sample from the surface of the lithium-rich salt lake sediment layer and shaking it thoroughly, the slag is removed and the concentrated bacterial solution is obtained by high-speed centrifugation. The concentrated solution is applied to the surface of the culture medium gel and placed in a CO2 incubator at 25°C for cultivation. The growth of Li-CO2-sensitive and salt-tolerant anaerobic fungi around the introduction point is observed microscopically, and a growth curve is drawn according to the change in the size of the bacterial plaque. Then, excellent mycelium is selected for further cultivation by the streaking method. After the isolated mycelium is obtained, the expansion is completed by the CO2 aeration method, and finally the strain required by this patent is obtained;

[0039] S1030: The Li-CO2-sensitive, salt-tolerant anaerobic fungi bred from lithium-rich salt lakes are salt-tolerant, low-temperature bacteria that meet the geological requirements for the construction of a CO2 adsorption layer near the constant temperature layer. The strain growth does not require external heating;

[0040] S1040: During the transition from nutritional inhibition to active growth, the growth of Li-CO2-sensitive salt-tolerant anaerobic fungi has the following characteristics: first, it undergoes a gradually accelerated growth process, and then gradually enters a balanced and stable growth process according to the growth curve and the actual supply rate of CO2. The crack plugging effect is significant, the CO2 supply decreases, the Li2CO3 hydrolysis effect weakens, and the Li and CO2 supply capacities decrease simultaneously. The growth rate of the fungus slows down due to nutritional restriction until the plugging is successful and CO2 escape is inhibited. The growth of Li-CO2-sensitive salt-tolerant anaerobic fungi enters a dormant state again due to nutritional inhibition, thus experiencing the process of "acceleration-constant speed-deceleration-inhibition waiting".

[0041] Furthermore, the auto-coupling and isolation repair is based on the escape of CO2 from the adsorption layer due to isolation failure as a basic condition, and the Li-CO2 dual conditions are completed by synchronously hydrolyzing Li2CO3 in the isolation layer technology implementation area with CO2; the auto-coupling and is the control of the repair process that is automatically completed by the implementation of this technology without changing the CO2 concentration in the isolation layer technology implementation area, and the growth control of Li-CO2 sensitive salt-tolerant anaerobic fungi is also based on the escape of CO2 as the basic control condition, and changes in the concentration of a single water-soluble LiHCO3 or CO2 cannot meet the nutritional needs of the growth of Li-CO2 sensitive salt-tolerant anaerobic fungi.

[0042] The working principle of the present invention is specifically described as follows:

[0043] Li salt culture medium and Li-CO2 sensitive salt-tolerant anaerobic fungi are sprayed at a thickness of 5 meters on the outer surface of the CO2 adsorption layer isolation layer to form an isolation layer technology implementation area. The initial Li salt culture medium contains a high concentration of insoluble Li2CO3, but the concentrations of water-soluble LiHCO3 and CO2 in the isolation layer technology implementation area are too low to meet the growth conditions of Li-CO2 sensitive salt-tolerant anaerobic fungi, resulting in the inability to grow; when cracks appear in the CO2 adsorption layer isolation, a large amount of adsorbed CO2 escapes and passes through the spray layer. The escaped CO2 will accelerate the Li2CO 3 is hydrolyzed into LiHCO3 and diffuses around. The Li concentration and CO2 concentration in the liquid environment increase at the same time. Supplemented with N, P, K and other elements of the Li salt culture medium in the low lithium salt state, the growth conditions of Li-CO2 sensitive salt-tolerant anaerobic fungi are met, and they grow rapidly to fill the crack space and gradually complete the crack plugging process. The crack plugging speed has the characteristics of "acceleration-constant speed-deceleration-inhibition waiting". During the waiting period, Li-CO2 sensitive salt-tolerant anaerobic fungi are in a semi-inhibited state, waiting for the arrival of the next high CO2 escape cycle.

[0044] The technical implementation area has active environmental control characteristics: the technical principle is that since the Li salt culture medium only contains insoluble Li2CO3, there is no free CO2 around the well-isolated technical implementation area, and the hydrolysis of Li2CO3 cannot be completed, so that the isolation layer technical implementation area lacks water-soluble Li elements and C sources at the same time, and does not meet the basic growth nutritional conditions of Li-CO2 sensitive salt-tolerant anaerobic fungi; when only the LiHCO3 concentration is higher than the critical concentration of 5g / L and there is a continuous supply of CO2, the growth conditions of Li-CO2 sensitive salt-tolerant anaerobic fungi are met; therefore, when the technical implementation area is well isolated, a patented method implementation area with waiting characteristics is formed at a 5-meter thickness position on the outer facade of the CO2 adsorption layer isolation layer, and the startup of the patented technology is controlled by the failure of isolation.

[0045] Li salt culture medium is a non-water-soluble culture medium with key ingredients and has CO2 coupling switch characteristics. The culture medium is basically evenly dispersed in the technical implementation area. When the isolation fails and CO2 escapes, Li2CO3 is hydrolyzed into water-soluble LiHCO3 in a humid environment, and diffuses to the surrounding area accompanied by surface water, thereby supplementing the water-soluble Li element in the technical implementation area. Furthermore, if the LiHCO3 in the technical implementation area decreases, Li2CO3 is hydrolyzed and supplemented in real time, so that the area is always kept in a nearly saturated LiHCO3 state, meeting the growth requirements of Li-CO2-sensitive and salt-tolerant anaerobic fungi, thereby entering an active state.

[0046] Li-CO2 sensitive salt-tolerant anaerobic fungi are obtained from lithium-rich salt lakes. The specific method is as follows: Li-CO2 sensitive salt-tolerant anaerobic fungi screening medium is prepared based on the Li salt medium formula, and the LiHCO3 concentration is controlled to form three Li gradient mediums, and the LiHCO3 concentrations are set to 100% saturation concentration, half-saturation concentration, and 20% concentration, respectively, and made into gels, which are coated in culture dishes; after the brine sample is absorbed from the surface of the sediment layer of the lithium-rich salt lake and fully shaken, the concentrated bacterial liquid is obtained by high-speed centrifugation after the slag is removed, and the concentrated liquid is applied to the surface of the culture medium gel, placed in a CO2 incubator, and cultivated at 25°C. The growth of Li-CO2 sensitive salt-tolerant anaerobic fungi around the introduction point is observed microscopically, and a growth curve is drawn according to the change in plaque size, and then excellent mycelium is selected for further cultivation by streaking method, and after obtaining isolated mycelium, the expansion is completed by CO2 aeration method, and finally the strains required for this patent are obtained. The Li-CO2 sensitive salt-tolerant anaerobic fungi selected from the lithium-rich salt lake are salt-tolerant low-temperature bacteria, which meet the geological conditions for the construction of the CO2 adsorption layer near the constant temperature layer, and the strain growth does not require external heating. Furthermore, in the process of Li-CO2 sensitive salt-tolerant anaerobic fungi turning from nutritional inhibition to active growth, its growth has the following characteristics: first, it undergoes a gradually accelerated growth process, and gradually enters a balanced and stable growth process according to the growth curve and the actual supply rate of CO2. With the significant effect of crack plugging, the CO2 supply decreases, the Li2CO3 hydrolysis effect weakens, and the Li and CO2 supply capacity decreases simultaneously. The growth rate of the strain slows down due to nutritional restriction until the plugging is successful, CO2 escape is inhibited, and the growth of Li-CO2 sensitive salt-tolerant anaerobic fungi enters a dormant state again due to nutritional inhibition, thus experiencing the process of "acceleration-constant speed-deceleration-inhibition waiting".

[0047] The auto-coupling and isolation repair is based on the basic condition of CO2 escape from the adsorption layer caused by isolation failure, and the Li-CO2 dual conditions are completed by synchronously hydrolyzing Li2CO3 in the isolation layer technology implementation area with CO2; the auto-coupling and is the patented technology that automatically completes the control of the repair process without changing the CO2 concentration in the isolation layer technology implementation area, and the growth control of Li-CO2 sensitive salt-tolerant anaerobic fungi is also based on CO2 escape as the basic control condition. Changes in the concentration of a single water-soluble LiHCO3 or CO2 cannot meet the nutritional needs of the growth of Li-CO2 sensitive salt-tolerant anaerobic fungi.

[0048] Embodiment 1:

[0049] The following is a specific implementation method of a deep formation CO2 adsorption storage isolation failure auto-coupling repair method of the present invention, which is described in detail as follows:

[0050] (1) Strata selection and analysis:

[0051] Investigate and analyze the geological conditions of the CO2 adsorption layer (3) used for carbon storage, determine the stability of the geological structure around the CO2 adsorption layer and the safety of the storage area, and determine the thickness of the isolation zone. In this embodiment, the isolation layer is designed to be 8m thick (0), of which the bottom layer of 3m is (2) the foundation layer, and the overlying 5m is the technical implementation area (1), and above the technical implementation area (1) is the overlying layer and the connecting stratum (4).

[0052] The geological environment requires that the area around the CO2 adsorption layer is humid and the hydrogeological conditions are stable, there is no underground water system with large flow volume in the surrounding area; the ground temperature is 25-60℃; there is no free CO2 reservoir in the surrounding strata.

[0053] (2) Obtaining Li-CO2 sensitive salt-tolerant anaerobic fungi concentrated solution:

[0054] Take sediment samples from the surface of the lithium-rich salt lake sediment layer and observe whether there are a large number of long rod-shaped or filamentous fungi. In the area where the salt lake fungi are rich, take 1L of brine sample from the surface of the sediment layer;

[0055] The sample was fully shaken in the ultra-clean environment of the laboratory, and after standing for 30 seconds, large particles of minerals were removed and the supernatant was taken;

[0056] Dispense into 100 mL centrifuge tubes, centrifuge at 12,000 rpm for 15 min, remove the supernatant, and keep 1 mL;

[0057] After vortexing, a total of 10 mL of sample was obtained. Centrifugation was continued and 9 mL of supernatant was discarded. The remaining 1 mL was the concentrated bacterial solution.

[0058] (3) Preparation of screening medium for Li-CO2 sensitive and salt-tolerant anaerobic fungi

[0059] Based on the formula of Li salt culture medium, gradient culture medium with different LiHCO3 concentrations (saturated solubility, half-saturated solubility, 20% LiHCO3) was prepared and made into gel, which was coated in the culture dish with the coating thickness controlled at 3-5mm. The configuration methods of the three culture media are shown in Table 1:

[0060] Table 1 Components of the screening medium for salt-tolerant anaerobic fungi with Li-CO2 sensitivity at gradient Li concentration

[0061]

[0062]

[0063] (4) Breeding of Li-CO2 sensitive and salt-tolerant anaerobic fungi

[0064] Use a sampling needle to inject the concentrated bacterial solution obtained in step 3 onto the surface of the culture medium gel, place it in a CO2 incubator, and culture it at 25°C;

[0065] The growth of Li-CO2-sensitive salt-tolerant anaerobic fungi around the soil was observed microscopically every 12 hours. Ten representative plaques were selected to calculate the change in plaque diameter and find the average value. A growth curve was drawn based on the change in plaque size ( Figure 2 ).

[0066] The plaques with good growth at 100% and 50% concentrations were selected. Figure 2 Two gray growth curves), and compared with the experimental group with significantly lower plaque growth at 20% Li concentration ( Figure 2 black growth curve).

[0067] The plaques of 100% of the experimental group were further selected as bacterial sources by streaking method and further cultivated by the same method;

[0068] The strains with good growth and full plaque were selected as the preferred bacterial sources, and further cultivated by liquid phase cultivation method. The medium used was Li-CO2 sensitive salt-tolerant anaerobic fungi screening medium with saturated LiHCO3 concentration (peptone removed), and the aeration volume was 50mL / L / min. Finally, the strains that met the patent requirements were obtained (the bacterial abundance was required to be greater than 1*10 8 / mL).

[0069] The Li-CO2 sensitive, salt-tolerant anaerobic fungi bred from lithium-rich salt lakes are salt-tolerant, low-temperature bacteria that meet the geological conditions required for the construction of a CO2 adsorption layer near the isothermal layer, and the strains do not require external heating for growth.

[0070] (5) Construction of Li salt culture medium on the facade of CO2 adsorption layer isolation layer and spraying of Li-CO2 sensitive salt-tolerant anaerobic fungi:

[0071] According to the isolation layer designed in step 1, the base layer is first constructed, and then the material is mixed with the Li salt culture medium at a ratio of 10:1. The bacterial source is sprayed with a layer of Li-CO2 sensitive salt-tolerant anaerobic fungi (1-2) with a 1-meter thick isolation layer (1-1) (spraying amount: 500mL / m 2 、Bacteria abundance 1*10 8 The construction of the 5m thick isolation layer technology implementation area (1) was completed. The amount of culture medium and bacteria added is shown in Table 2.

[0072] Table 2 Li salt culture medium

[0073]

[0074]

[0075] Preparation of Li salt culture medium: In the initial state, the culture medium contains a high concentration of Li2CO3 (60g / L) to inhibit the growth of Li-CO2 sensitive and salt-tolerant anaerobic fungi and put them in a dormant state.

[0076] (6) Formation of cracks in the isolation layer of CO2 adsorption layer and hydrolysis of Li2CO3:

[0077] When cracks appear in the isolation layer (0) of the CO2 adsorption layer, CO2 escapes and passes through the technical implementation area (1) one by one.

[0078] The humid stratum environment releases CO2 that hydrolyzes solid Li2CO3 into water-soluble LiHCO3, which diffuses to the surrounding area along with the stratum water. The chemical process of Li2CO3 hydrolysis is as follows:

[0079] Li2CO3+CO2+H2O=2LIHCO3

[0080] (7) Li-CO2 sensitive salt-tolerant anaerobic fungi growth and crack repair:

[0081] As the concentration of LiHCO3 in the technical implementation area (1) increases, the growth conditions of Li-CO2-sensitive salt-tolerant anaerobic fungi are met with the help of the escaped CO2, thereby breaking the nutritional inhibition of Li-CO2-sensitive salt-tolerant anaerobic fungi.

[0082] Li-CO2-sensitive, salt-tolerant anaerobic fungi begin to grow rapidly in the crack space through the supply of water-soluble LiHCO3 and sufficient CO2 environment, supplemented by Li salt culture medium as a source of nutrients such as N, P, and K. The growth rate is related to the supply of Li elements and CO2. The cracks are gradually filled through the growth of hyphae, and under the influence of the pressure of the escaped CO2, the hyphae are gradually compacted in the cracks, completing the crack sealing process.

[0083] During the transition from nutritional inhibition to active growth, the growth of Li-CO2-sensitive salt-tolerant anaerobic fungi has the following characteristics: first, it undergoes a gradually accelerated growth process, and then gradually enters a balanced and stable growth process according to the growth curve and the actual supply rate of CO2. With the significant effect of fissure plugging, the CO2 supply decreases, the Li2CO3 hydrolysis weakens, and the Li and CO2 supply capacities decrease simultaneously. The growth rate of the fungus slows down due to nutritional restriction until the plugging is successful and CO2 escape is inhibited. The growth of Li-CO2-sensitive salt-tolerant anaerobic fungi enters a dormant state again due to nutritional inhibition, thus experiencing the process of "acceleration-constant speed-deceleration-inhibition waiting".

[0084] (8) Environmental adaptability and restoration effect assessment:

[0085] In practical applications, we can first simulate the formation of cracks caused by pile aging or geological activities through experiments, and monitor the "acceleration-constant speed-deceleration-inhibition waiting" process of Li-CO2 sensitive salt-tolerant anaerobic fungi in the growth process under experimental conditions to ensure that they grow rapidly and fill the cracks in the early stage of crack repair, and enter a dormant state after the cracks are successfully sealed. In this way, the repair effect of Li-CO2 sensitive salt-tolerant anaerobic fungi in a CO2 escape environment can be evaluated, and then used on site.

[0086] When an abnormal CO2 level is found in a certain area on site, real-time monitoring is carried out. If the CO2 level drops to normal within 7 days, it means that the patented technology has been implemented. Otherwise, the cracks may be too large to achieve abundance by relying on bacterial filling, and other technologies may be needed to complete the repair of the isolation area (0).

[0087] By long-term monitoring of the stability of surrounding CO2, it is verified that the effect of Li-CO2 sensitive and salt-tolerant anaerobic fungi in repairing cracks is long-lasting, ensuring the long-term stability and sealing effect of the CO2 adsorption layer.

[0088] If the Li2CO3 in the technical implementation area (1) is exhausted, it is necessary to first evaluate the reliability of the isolation at that point, strengthen the isolation of the area through necessary technical measures, and replenish the Li salt culture medium in a timely manner to ensure the reliability of the isolation.

[0089] Through the above-mentioned specific implementation methods, the deep formation CO2 adsorption storage isolation failure auto-coupling repair method of the present invention can effectively solve the isolation weakening or failure problem caused by pile aging or geological activities during underground CO2 adsorption storage, and realize efficient, environmentally friendly and adaptive crack repair, which has significant technical advantages and application prospects.

[0090] The deep formation CO2 adsorption storage isolation failure auto-repair method provided by the present invention has the following beneficial effects:

[0091] (1) Adaptive repair: By utilizing the self-coupling and characteristics of the Li salt culture medium with the help of hydrolysis reaction when CO2 escapes, the repair process can be automatically started when cracks appear in the CO2 adsorption layer, achieving adaptive sealing of the cracks and ensuring the long-term stability of the isolation layer.

[0092] (2) Environmental protection and high efficiency: This method avoids the use of chemical substances or complex physical remediation methods and adopts bioremediation technology, which is not only environmentally friendly, but also low-cost, easy to operate, and suitable for large-scale application.

[0093] (3) Dynamic control and timely high repair efficiency: The size of the cracks in the isolation layer is related to the amount of CO2 escaping, and CO2 is related to the Li2CO3 hydrolysis reaction and the C source supply of Li-CO2-sensitive salt-tolerant anaerobic fungi. This achieves dynamic control of the growth of Li-CO2-sensitive salt-tolerant anaerobic fungi. The larger the cracks and the more sufficient the supply of Li and C elements, the faster the growth rate of Li-CO2-sensitive salt-tolerant anaerobic fungi, and the higher the crack filling rate, which has the characteristics of timely and efficient crack repair. At the same time, after the crack repair is completed, it can enter a dormant state to avoid excessive growth.

[0094] (4) Reduce maintenance costs: Since Li-CO2-sensitive salt-tolerant anaerobic fungi enter a dormant state after the crack repair is completed, they wait for the next CO2 escape to restart the repair. At the same time, they also interrupt the Li2CO3 hydrolysis reaction, reducing the need for frequent maintenance and reducing long-term operation and maintenance costs.

[0095] The above description is only a preferred embodiment of the present invention, so all equivalent changes or modifications made according to the features and principles described in the scope of the patent application of the present invention are included in the scope of the patent application of the present invention.

Claims

1. A method for self-coupling repair of CO2 adsorption storage isolation failure in deep formations, characterized by: Use the following steps: S10: Spraying Li salt culture medium and Li-CO2 sensitive salt-tolerant anaerobic fungi at a thickness of 5 meters on the outer surface of the CO2 adsorption layer isolation layer to form an isolation layer technology implementation area; S20: The initial Li salt culture medium contains a high concentration of insoluble Li2CO3, but the concentrations of water-soluble LiHCO3 and CO2 in the isolation layer technology implementation area are too low to meet the growth conditions of Li-CO2 sensitive salt-tolerant anaerobic fungi, resulting in failure to grow; S30: When cracks appear in the isolation of the CO2 adsorption layer, a large amount of adsorbed CO2 escapes. When passing through the spray layer, the escaped CO2 will accelerate the hydrolysis of Li2CO3 into LiHCO3 and diffuse around. The Li concentration and CO2 concentration in the liquid environment increase at the same time. Supplemented by the N, P, K and other elements of the Li salt culture medium in the low lithium salt state, the growth conditions of Li-CO2 sensitive salt-tolerant anaerobic fungi are met, and they grow rapidly to fill the crack space and gradually complete the crack plugging process. The crack plugging speed has the characteristics of "acceleration-constant speed-deceleration-inhibition waiting". During the waiting period, the Li-CO2 sensitive salt-tolerant anaerobic fungi are in a semi-inhibited state, waiting for the arrival of the next high CO2 escape cycle.

2. According to claim 1, a deep formation CO2 adsorption storage isolation failure auto-repair method is characterized by: The isolation layer technology implements active control characteristics of the zone environment: Since the Li salt culture medium contains insoluble Li2CO3, there is a lack of free CO2 around the well-isolated technical implementation area, and the hydrolysis of Li2CO3 cannot be completed, so that the isolation layer technical implementation area lacks water-soluble Li elements and C sources at the same time, and does not meet the basic growth nutritional conditions of Li-CO2 sensitive salt-tolerant anaerobic fungi; The growth conditions of Li-CO2-sensitive salt-tolerant anaerobic fungi are met only when the LiHCO3 concentration is higher than the critical concentration of 5g / L and there is a continuous supply of CO2; thus, when the technical implementation area is well isolated, a patented method implementation area with waiting characteristics is formed at a 5-meter-thick position on the outer facade of the CO2 adsorption layer isolation layer, with isolation failure as the control condition.

3. According to claim 1, a deep formation CO2 adsorption storage isolation failure auto-repair method is characterized by: The Li salt culture medium is a non-water-soluble culture medium with a key ingredient and has a CO2 coupled switch characteristic. The culture is basically evenly dispersed in the technical implementation area. When the isolation fails and CO2 escapes, Li2CO3 is hydrolyzed into water-soluble LiHCO3 in a humid environment, and diffuses to the surrounding area accompanied by surface water, thereby supplementing the water-soluble Li element in the technical implementation area. Furthermore, if the LiHCO3 in the technical implementation area decreases, Li2CO3 is hydrolyzed and supplemented in real time, so that the area is always kept in a nearly saturated LiHCO3 state, meeting the growth requirements of Li-CO2-sensitive and salt-tolerant anaerobic fungi, thereby entering an active state.

4. According to claim 1, a deep formation CO2 adsorption storage isolation failure auto-repair method is characterized by: The Li-CO2 sensitive salt-tolerant anaerobic fungi in step S10 are obtained from a lithium-rich salt lake. The specific method is: S1010: A Li-CO2 sensitive salt-tolerant anaerobic fungus screening medium is prepared based on the Li salt medium formula, and the LiHCO3 concentration is controlled to form three Li gradient mediums, wherein the LiHCO3 concentrations are set to 100% saturation concentration, half-saturation concentration, and 20% concentration, respectively, and made into a gel and applied to a culture dish; S1020: After absorbing the brine sample from the surface of the lithium-rich salt lake sediment layer and shaking it thoroughly, the slag is removed and the concentrated bacterial solution is obtained by high-speed centrifugation. The concentrated solution is applied to the surface of the culture medium gel and placed in a CO2 incubator at 25°C for cultivation. The growth of Li-CO2-sensitive and salt-tolerant anaerobic fungi around the introduction point is observed microscopically, and a growth curve is drawn according to the change in the size of the bacterial plaque. Then, excellent mycelium is selected for further cultivation by the streaking method. After the isolated mycelium is obtained, the expansion is completed by the CO2 aeration method, and finally the strain required by this patent is obtained; S1030: The Li-CO2-sensitive, salt-tolerant anaerobic fungi bred from lithium-rich salt lakes are salt-tolerant, low-temperature bacteria that meet the geological requirements for the construction of a CO2 adsorption layer near the constant temperature layer. The strain growth does not require external heating; S1040: During the transition from nutritional inhibition to active growth, the growth of Li-CO2-sensitive salt-tolerant anaerobic fungi has the following characteristics: first, it undergoes a gradually accelerated growth process, and then gradually enters a balanced and stable growth process according to the growth curve and the actual supply rate of CO2. The crack plugging effect is significant, the CO2 supply decreases, the Li2CO3 hydrolysis effect weakens, and the Li and CO2 supply capacities decrease simultaneously. The growth rate of the fungus slows down due to nutritional restriction until the plugging is successful and CO2 escape is inhibited. The growth of Li-CO2-sensitive salt-tolerant anaerobic fungi enters a dormant state again due to nutritional inhibition, thus experiencing the process of "acceleration-constant speed-deceleration-inhibition waiting".

5. According to claim 1, a deep formation CO2 adsorption storage isolation failure auto-repair method is characterized by: The self-coupling and isolation repair is based on the basic condition of CO2 escape from the adsorption layer caused by isolation failure, and the Li-CO2 dual conditions are completed by synchronously hydrolyzing Li2CO3 in the isolation layer technology implementation area with CO2; the self-coupling and is the control of the repair process automatically completed by the implementation of this technology with the CO2 concentration in the isolation layer technology implementation area unchanged, and the growth control of Li-CO2 sensitive salt-tolerant anaerobic fungi is also based on CO2 escape as the basic control condition, and the change of single water-soluble LiHCO3 or CO2 concentration cannot meet the nutritional needs of Li-CO2 sensitive salt-tolerant anaerobic fungi for growth.

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