Graphite-phase carbon material for adsorbing and removing mycotoxin in food as well as preparation method and application of graphite-phase carbon material

Abstract

The invention belongs to the technical field of materials and harmful pollutant removal, and particularly relates to a graphite-phase carbon material for adsorbing and removing mycotoxin in food as well as a preparation method and application of the graphite-phase carbon material. The preparation method of the graphite-phase carbon material comprises the following steps: at normal temperature and normal pressure, ball-milling graphite or a mixture of graphite and ferroferric oxide into solid powder, so as to prepare the graphite-phase carbon material for adsorbing and removing mycotoxin in food. The graphite phase carbon material is good in adsorption effect; the composition can be used for preparing aflatoxin, zearalenone, ochratoxin, deoxynivalenol and T-2 toxin in various oils such as peanut oil, soybean oil, corn germ oil, rapeseed oil, sesame oil, walnut oil, linseed oil, rice bran oil, wheat germ oil, edible plant blend oil, sunflower seed oil, olive oil, camellia oleosa seed oil, perilla seed oil and almond oil. And various fungaltoxins such as fumonisins are removed, so that the pollution level of the fungaltoxins in the edible vegetable fat can be effectively reduced.

Description

Technical Field

[0001] The invention belongs to the technical field of materials and harmful pollutant removal, and specifically relates to a graphite phase carbon material for adsorbing and removing mycotoxins in food, and a preparation method and application thereof. Background Art

[0002] Mycotoxins are secondary metabolites with biological toxicity produced by fungi. They are a class of natural pollutants that have attracted much attention. They mainly contaminate common foods in human life, such as grains, crops, and dairy products, and seriously threaten human health. According to statistics, about 25% of the world's food crops are contaminated by mycotoxins, and in my country, 3,100 tons of food is contaminated by mycotoxins every year during production, storage, transportation, and sales. Common mycotoxins include aflatoxin B1, ochratoxin A, deoxynivalenol, zearalenone toxin, T-2 toxin, and fumonisin. These mycotoxins are very harmful to human health. Eating or contacting food contaminated with mycotoxins can cause acute or chronic toxicity to the health of humans and animals, and exposure to mycotoxins during pregnancy can increase the risk of adverse birth outcomes such as low birth weight, miscarriage, and malformations in the fetus. At the same time, the combined exposure to multiple mycotoxins can produce synergistic toxic effects on organisms, which has further aroused widespread public concern about the damage to human health caused by mycotoxin-contaminated food. Therefore, the food security issues caused by mycotoxin contamination cannot be ignored. It is necessary to develop a green, efficient and rapid mycotoxin reduction and detoxification technology to reduce the cost of traditional control technology, ensure the quality and safety of food crops and their products, and protect human health.

[0003] The control and reduction technologies for mycotoxins in food at home and abroad mainly include photodegradation, biological method, chemical method and physical adsorption method. Photodegradation can be used for the degradation of mycotoxins and has the characteristics of high efficiency, but the mineralization degree of traditional catalysts is not high enough and it is easy to produce highly toxic byproducts. Biological methods can be used for the degradation of various mycotoxins and have the advantages of simple operation, high efficiency and strong specificity, but its research mainly focuses on the screening of strains and degrading enzymes, and there are few reports on key scientific issues such as the adsorption and degradation removal mechanism of mycotoxins. Chemical methods have been widely used for the degradation of various mycotoxins in aqueous solutions. However, the detoxification treatment of mycotoxins by this method may cause changes in the characteristics of actual samples such as grains, affect the nutritional value and flavor of food, and there are safety hazards such as chemical reagent residues. Physical adsorption is considered to be the most promising method to reduce and eliminate mycotoxin contamination due to its advantages such as high efficiency, low cost and no highly toxic byproducts. Traditional adsorbent materials such as activated carbon, aluminosilicates, clay minerals, etc. have certain effects on the reduction of toxins, but they also have problems such as small specific surface area, low adsorption capacity and poor adsorption selectivity. Graphene is composed of a single layer of two-dimensional sp 2The honeycomb grid structure formed by hybrid carbon atoms has a large specific surface area, good dispersibility, and the graphene surface contains abundant oxygen-containing groups. It has the advantages of high surface activity and easy modification, and has gradually become a research hotspot in the field of adsorption and separation. In order to improve the interaction between graphene and fungal toxins, researchers have introduced functional active groups to the graphene surface using strategies such as covalent bond modification, non-covalent bond modification, element doping, and in-situ polymerization, and have developed a variety of graphene-based modified materials for the adsorption and removal of fungal toxins. However, the preparation methods of these graphene-based modified materials are relatively complex, time-consuming, costly, and have relatively low yields. They are not easy to prepare on a large scale, and the powdered adsorbents are difficult to recover from the solution after use, which also limits their practical application.

[0004] Due to the special magnetic response properties of magnetic materials, when an external magnetic field is applied, the magnetic adsorbent can quickly aggregate and settle and separate from the reaction solution. Compared with filtration, centrifugation and other methods, it can greatly save time, effectively reduce adsorbent loss, and avoid the loss of active components. Therefore, loading magnetism is an effective strategy to solve the problem that graphene adsorbents are difficult to recover and easy to lose when applied to the removal of fungal toxins. However, ferroferric oxide is commonly used as a magnetic carrier in the preparation of existing magnetic composite materials. Hydrothermal method, coprecipitation method and other methods are often used to prepare magnetic ferroferric oxide-graphene composite materials. The preparation of the composite material includes multiple continuous experimental steps such as the preparation of ferroferric oxide, the preparation of graphene, the preparation of graphene oxide, and the preparation of ferroferric oxide-graphene oxide composite materials. The overall preparation process is very cumbersome, time-consuming, low in yield, and uses a large amount of organic solvents, which is not environmentally friendly. In addition, studies have shown that the loading of ferroferric oxide in the above preparation process will cover some active sites of graphene to a certain extent. Compared with the adsorption efficiency of the graphene matrix before loading, the adsorption efficiency of the ferroferric oxide-graphene composite material will decrease. Therefore, considering the preparation process of adsorbent materials and the adsorption efficiency of mycotoxins in the prior art, it is difficult to apply them to the reduction and detoxification of mycotoxins in food and its products. Summary of the invention

[0005] In view of the problems and shortcomings in the prior art, the purpose of the present invention is to provide a graphite phase carbon material for adsorbing and removing fungal toxins in food, and a preparation method and application thereof.

[0006] Based on the above purpose, the present invention adopts the following technical solution:

[0007] The first aspect of the present invention provides a method for preparing a graphite-phase carbon material for adsorbing and removing fungal toxins in food. At room temperature and pressure, graphite or a mixture of graphite and ferroferric oxide is ball-milled into a solid powder to obtain a graphite-phase carbon material for adsorbing and removing fungal toxins in food.

[0008] Furthermore, in the mixture, the mass ratio of ferrosoferric oxide to graphite is (0.01-100):1.

[0009] Furthermore, the graphite is any one of flake graphite, expanded graphite and amorphous graphite.

[0010] Furthermore, the ball milling speed of the ball milling process is 100 to 1000 rpm, and the ball milling reaction time is 100 to 600 min.

[0011] The second aspect of the present invention provides a graphite phase carbon material for adsorbing and removing fungal toxins in food, which is prepared using the preparation method described in the first aspect.

[0012] The third aspect of the present invention provides the use of the graphite phase carbon material described in the second aspect in the adsorption and removal of fungal toxins in food.

[0013] Furthermore, the graphite phase carbon material is dispersed in the food sample and mixed, and then an adsorption reaction is carried out under stirring or shaking. After the adsorption reaction is completed, the graphite phase carbon material is separated from the food sample.

[0014] Furthermore, the food is vegetable oil, and the fungal toxin is one or more of aflatoxin, zearalenone, ochratoxin, deoxynivalenol, T-2 toxin, and fumonisin.

[0015] Furthermore, the vegetable oil is one or more of peanut oil, soybean oil, corn germ oil, rapeseed oil, sesame oil, walnut oil, linseed oil, rice bran oil, wheat germ oil, edible vegetable blended oil, sunflower seed oil, olive oil, camellia seed oil, perilla seed oil, and almond oil.

[0016] Compared with the prior art, the present invention has the following beneficial effects:

[0017] (1) The present invention uses low-cost graphite or a mixture of ferroferric oxide and graphite as a reactant, and adopts a green, mild and simple mechanochemical method at room temperature and pressure to obtain graphite phase carbon material and magnetic graphite phase carbon material in one step. The whole preparation process does not require any solvent, and the preparation process is simple, the raw materials are cheap, the preparation time is short, and it is easy to mass produce. The prepared carbon material has good solubility and dispersibility in the solution, and the adsorbed graphite phase carbon material can be separated from the adsorption system by centrifugation.

[0018] (2) In particular, the graphite phase carbon material added with ferroferric oxide has magnetic properties. Under the action of an external magnet, the material and the solution can be completely separated within 10 seconds, and the composite of the magnetic material does not cover the active sites on the surface of the carbon material; under the action of π-π bonds and hydrophobicity, the carbon material shows a good adsorption effect on fungal toxins.

[0019] (3) The graphite phase carbon material prepared by the technical solution provided by the present invention not only has a good adsorption effect on single or mixed fungal toxins in a pure solvent phase, but also has a significant adsorption effect on fungal toxins in actual samples such as various types of plant oils and fats. It has extremely high practical value and application prospects and is suitable for commercial promotion. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 This is a Raman spectrum (Raman) diagram of the graphite phase carbon material in Example 1 of the present invention.

[0021] Figure 2 This is the hysteresis loop of the magnetic graphite phase carbon material in Example 4 of the present invention.

[0022] Figure 3 This is a thermodynamic adsorption curve diagram of aflatoxin B1, ochratoxin A and zearalenone by the graphite phase carbon material in Example 1 of the present invention.

[0023] Figure 4 This is a kinetic adsorption curve diagram of aflatoxin B1, ochratoxin A and zearalenone by the graphite phase carbon material in Example 1 of the present invention.

[0024] Figure 5 The graphite phase carbon material in Example 1 of the present invention is a bar graph showing the adsorption rate of aflatoxin B1 and ochratoxin A in vegetable oils and fats such as peanut oil (1), soybean oil (2), corn germ oil (3), rapeseed oil (4), sesame oil (5), walnut oil (6), linseed oil (7), rice bran oil (8), wheat germ oil (9), edible vegetable blended oil (10), sunflower seed oil (11), olive oil (12), camellia oil (13), perilla seed oil (14), and almond oil (15). DETAILED DESCRIPTION

[0025] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below through embodiments in combination with the accompanying drawings. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0026] (I) Preparation of graphite-phase carbon materials for adsorption and removal of fungal toxins in food

[0027] Example 1

[0028] A method for preparing a graphite-phase carbon material for adsorbing and removing mycotoxins in food comprises the following steps:

[0029] (1) At room temperature and pressure, 5 g of flake graphite was added to a ball milling jar of a ball milling reactor, a combination of grinding balls of different diameters and weights was added to the ball milling jar, and a sealing ring was used to connect the ball milling cover to the ball milling jar;

[0030] (2) Fix the ball mill jar on the ball mill, set the speed of the ball mill to 300 rpm and the ball milling reaction time to 150 min. After the reaction is completed, take out the jar body and collect the solid powder in the jar body to obtain the graphite phase carbon material.

[0031] Example 2

[0032] A method for preparing a magnetic graphite phase carbon material for adsorbing and removing fungal toxins in food comprises the following steps:

[0033] (1) At room temperature and pressure, 4.95 g of ferroferric oxide and 0.05 g of flake graphite were added to a ball milling jar of a ball milling reactor, a combination of grinding balls of different diameters and weights were added to the ball milling jar, and a sealing ring was used to connect the ball milling cover to the ball milling jar;

[0034] (2) Fix the ball mill jar on the ball mill, set the rotation speed of the ball mill to 300 rpm and the ball milling reaction time to 140 min. After the reaction is completed, take out the jar body and collect the solid powder in the jar body to obtain the magnetic graphite phase carbon material.

[0035] Example 3

[0036] The content of a preparation method of a magnetic graphite phase carbon material for adsorbing and removing fungal toxins in food is basically the same as that of Example 2, except that the amount of ferrosoferric oxide is 4.8 g, the amount of flake graphite is 0.2 g, and the ball milling reaction time is 200 min.

[0037] Example 4

[0038] The content of the preparation method of a magnetic graphite phase carbon material for adsorbing and removing fungal toxins in food is basically the same as that of Example 2, except that the ball milling reaction time is 300 minutes.

[0039] (II) Study on the adsorption performance of graphite phase carbon materials obtained under different preparation conditions on fungal toxins

[0040] The graphite phase carbon material prepared in Examples 1 to 4 was evaluated for the adsorption performance of mycotoxins. The specific experimental process was as follows: a certain mass of aflatoxin B1, ochratoxin A and zearalenone were weighed and dissolved in methanol to prepare a mycotoxin stock solution with a concentration of 10 mg / L, and then the stock solution was diluted with a mixed solvent of methanol and deionized water with a volume ratio of 30:70 to obtain a mixed mycotoxin working solution with a concentration of 0.1 mg / L; 3 mg of the graphite phase carbon material prepared in Examples 1 to 4 was weighed in a 2 mL centrifuge tube, 1 mL of 0.1 mg / L mixed mycotoxin working solution was added thereto, and after oscillation at room temperature for 12 hours, the adsorbent was centrifuged or separated rapidly from the solution under the action of an external magnet, the supernatant was taken and the mycotoxin content in the supernatant was determined by HPLC, and the equilibrium adsorption rate of the graphite phase carbon material to mycotoxin was calculated. The specific adsorption results are shown in Table 1.

[0041] Table 1 Adsorption of mycotoxins by graphite phase carbon materials prepared in Examples 1 to 4

[0042]

[0043]

[0044] As can be seen from Table 1, the graphite phase carbon materials prepared in Examples 1 to 4 all showed good adsorption effects on aflatoxin B1, ochratoxin A and zearalenone, with adsorption rates of 53.7% to 93.5%. The above results show that the graphite phase carbon material prepared by the present invention can effectively remove a variety of mycotoxins, and confirm that the technical solution proposed by the present invention can prepare a graphite phase carbon material adsorbent and be used for the adsorption and removal of mycotoxins, achieving a good adsorption effect.

[0045] (III) Structural characterization of graphite phase carbon materials

[0046] Raman spectroscopy was used to characterize the defect types of the graphite phase carbon material prepared in Example 1, such as Figure 1 As shown, the material is at 1350cm -1 and 1589cm -1 The strong D peak and G peak appear at the , which are typical characteristic peaks of graphite phase carbon materials, verifying the successful preparation of graphite phase carbon materials. D / I G =0.89, indicating that there are defects in the prepared graphite phase carbon material, which may be due to the more thorough reduction caused by friction and collision during the ball milling process, further increasing the defects of the graphite layer structure. Figure 2It is the hysteresis loop of the magnetic graphite phase carbon material prepared in Example 4. As can be seen from the figure, the magnetic graphite phase carbon material does not show superparamagnetism, and the final saturation magnetization reaches 59.0emu / g, which is slightly lower than the traditional bare nanometer ferroferric oxide particles (67.47emu / g), mainly due to the presence of graphite in the composite material weakening the magnetism of ferroferric oxide to a certain extent. In the adsorbent separation test, the complete separation of the material and the solution can be achieved within 10s by the action of an external magnet, indicating that the prepared magnetic graphite phase carbon material can meet the separation requirements.

[0047] (IV) Study on the adsorption process of mycotoxins by graphite-phase carbon material adsorbent

[0048] The graphite phase carbon material adsorbent prepared in Example 1 is used as the experimental material to study the adsorption process of fungal toxins by the material of the present invention.

[0049] 1. Study on thermodynamic adsorption performance

[0050] (1) Experimental methods

[0051] A certain mass of aflatoxin B1, ochratoxin A and zearalenone were weighed and dissolved in methanol to prepare a mixed stock solution of mycotoxins with a concentration of 10 mg / L. The stock solution was then diluted with a mixed solvent of methanol and deionized water with a volume ratio of 30:70 to prepare a series of mixed working solutions of mycotoxins with concentrations of 2, 5, 10, 20, 50, 100, 200 and 500 μg / L, respectively. 3 mg of graphite phase carbon material was accurately weighed and placed in a 2 mL centrifuge tube, and then 1 mL of the mixed working solution of mycotoxins with different concentrations was added thereto, and oscillated and adsorbed at room temperature for 12 h. Centrifugation was performed to quickly separate the adsorbent and the solution, the supernatant was taken and the mycotoxin contents in the supernatant were determined by HPLC, and the saturated adsorption capacity of the graphite phase carbon material for the three mycotoxins was calculated.

[0052] (2) Results and analysis

[0053] Figure 3 The thermodynamic adsorption curves of graphite phase carbon materials for three fungal toxins ( Figure 3 a is the adsorption rate of graphite phase carbon material to fungal toxins; Figure 3b is the adsorption amount of the graphite phase carbon material to the fungal toxin). As shown in the figure, within the fungal toxin concentration range of 2 to 500 μg / L, the adsorption rate of the graphite phase carbon material to aflatoxin B1 in the mixed standard is 99.0 to 100%, the adsorption rate to ochratoxin A in the mixed standard is 99.0 to 100%, and the adsorption rate to zearalenone in the mixed standard is 47.1 to 99.7%. The adsorption amount of the three fungal toxins by the graphite phase carbon material increases linearly with the increase of the initial concentration of the fungal toxins. The results show that the graphite phase carbon material prepared by the present invention exhibits excellent adsorption capacity for the mixed standard of the three fungal toxins, which indicates that the graphite phase carbon material prepared by the present invention has relatively more active sites on the surface, which can meet the simultaneous high-capacity adsorption of the three fungal toxins and has not reached saturation. This also reflects that within a certain range, the initial concentration of the fungal toxin continues to increase, and there are still enough active sites on the surface of the graphite phase carbon material to achieve efficient adsorption of mixed fungal toxins at the same time.

[0054] 2. Kinetic adsorption performance study

[0055] (1) Experimental methods

[0056] The kinetic adsorption removal effect of the graphite phase carbon material prepared in Example 1 on the three mycotoxin mixture was studied, and the results are as follows: Figure 4 shown.

[0057] The specific experimental operation is as follows: a certain mass of aflatoxin B1, ochratoxin A and zearalenone are weighed and dissolved in methanol to prepare a mixed stock solution of mycotoxins with a concentration of 10 mg / L, and then the stock solution is diluted with a mixed solvent of methanol and deionized water with a volume ratio of 30:70 to prepare a mixed working solution of mycotoxins with a concentration of 100 μg / L; 3 mg of graphite phase carbon material is accurately weighed and placed in 2 mL centrifuge tubes, and then 1 mL of 100 μg / L mixed working solution of mycotoxins is added thereto, and oscillated adsorption is performed at room temperature for different reaction times (10 s, 20 s, 30 s, 1 min, 3 min, 5 min, 7 min, 10 min); centrifugation is performed to quickly separate the adsorbent and the solution, the supernatant is taken and the mycotoxin content in the supernatant is determined by HPLC, and the saturated adsorption capacity of the graphite phase carbon material for the three mycotoxins is calculated.

[0058] (2) Results and discussion

[0059] Figure 4 The kinetic adsorption data of three fungal toxins by the graphite phase carbon material in Example 1 of the present invention ( Figure 4 a is the adsorption rate of graphite phase carbon material to fungal toxins; Figure 4b is the adsorption amount of the graphite phase carbon material on the fungus toxin). It can be seen from the figure that within 0 to 10 minutes, the adsorption rate and adsorption amount of the graphite phase carbon material on the three fungus toxins increase significantly with the extension of the adsorption time. After only 10 seconds of adsorption, the adsorption rates of aflatoxin B1, zearalenone and ochratoxin A in the three mixed fungi by the graphite phase carbon material can reach 83.4%, 82.8% and 46.5%; in just 1 minute, the adsorption of aflatoxin B1 and zearalenone in the three mixed fungi by the graphite phase carbon material basically reaches the adsorption-desorption equilibrium. After 5 minutes of adsorption, the adsorption of ochratoxin A also reaches the adsorption-desorption equilibrium. Compared with other prior arts, the graphite phase carbon material prepared by the technical solution provided by the present invention shows a faster adsorption speed and higher adsorption efficiency for mixed fungi.

[0060] (V) Study on the adsorption performance of graphite phase carbon material adsorbents on fungal toxins in plant oils and fats

[0061] The graphite phase carbon material adsorbent prepared in Example 1 is used as an experimental material to study the adsorption capacity of the material of the present invention for fungal toxins in food.

[0062] Experimental Group 1:

[0063] (1) Preparation of peanut oil spiked sample: Weigh a certain amount of aflatoxin B1 and ochratoxin A respectively and dissolve them in methanol to prepare a mixed stock solution of mycotoxins with a concentration of 100 mg / L. Then dilute the stock solution with a mixed solvent of methanol and deionized water with a volume ratio of 90:10 to prepare a 5 mg / L aflatoxin B1 working solution and a 2.5 mg / L ochratoxin A working solution. Add 10 μL of the 5 mg / L aflatoxin B1 working solution and 10 μL of the 2.5 mg / L ochratoxin A working solution to 1 g of peanut oil to prepare a peanut oil spiked sample containing a mixed mycotoxin concentration of 50 μg / kg aflatoxin B1 and 25 μg / kg ochratoxin A.

[0064] (2) Adsorption experiment: 20 mg of graphite phase carbon material was accurately weighed and placed in 2 mL centrifuge tubes, and then 1 g of peanut oil spiked with fungal toxins was added thereto. The adsorption reaction was oscillated at room temperature for 60 min. Centrifugation was performed to separate the adsorbent from the oil. 0.5 g of the oil supernatant was taken out, and 2 mL of 90% methanol aqueous solution was added thereto. The mixture was vortexed and mixed. After shaking for 1 h, the mixture was centrifuged at 8000 rpm for 5 min. The supernatant was taken out and the contents of the two fungal toxins in the supernatant were determined by HPLC. The adsorption effect of the graphite phase carbon material on the two fungal toxins in peanut oil was calculated.

[0065] The experimental methods of experimental groups 2 to 15 are basically the same as those of experimental group 1. The difference is that in the preparation of oil-spiked samples, the vegetable oils selected in experimental groups 2 to 15 are soybean oil, corn germ oil, rapeseed oil, sesame oil, walnut oil, linseed oil, rice bran oil, wheat germ oil, edible vegetable blended oil, sunflower seed oil, olive oil, camellia seed oil, perilla seed oil, and almond oil.

[0066] The adsorption results of mycotoxins in experimental groups 1 to 15 are shown in Table 2.

[0067] Table 2 Adsorption effect of graphite phase carbon materials prepared in Example 1 on fungal toxins in vegetable oils

[0068]

[0069]

[0070] Display the data in Table 2 in the form of a bar chart, such as Figure 5 As shown, the experimental results of experimental groups 1 to 15 show that the graphite phase carbon material prepared by the present invention is respectively applied to the removal of fungal toxins in peanut oil, soybean oil, corn germ oil, rapeseed oil, sesame oil, walnut oil, linseed oil, rice bran oil, wheat germ oil, edible vegetable blended oil, sunflower oil, olive oil, camellia oil, perilla seed oil, and almond oil. The results show that aflatoxin in peanut oil, soybean oil, corn germ oil, rapeseed oil, sesame oil, walnut oil, linseed oil, rice bran oil, wheat germ oil, edible vegetable blended oil, sunflower oil, olive oil, camellia oil, perilla seed oil, and almond oil The removal rates of aflatoxin B1 and ochratoxin A can reach 97.2-100% and 90.0-100%, respectively. At this time, the residual concentrations of aflatoxin B1 and ochratoxin A in the above oils are 1.4-0 μg / kg and 2.5-0 μg / kg, respectively, which are far lower than the limit values ​​of aflatoxin B1 in soybean oil (20 μg / kg) and other vegetable oils (10 μg / kg) and the limit values ​​of ochratoxin A in cereals and their products (5 μg / kg) stipulated in the national food safety standard GB2761-2017 "Limits of Fungal Toxins in Foods". These results show that the graphite phase carbon material adsorbent prepared by the technical solution provided by the present invention can effectively detoxify aflatoxins and ochratoxin A in a variety of edible oils, and has great application prospects in the reduction and control of fungal toxins in vegetable oils.

Claims

1. A method for preparing a graphite-phase carbon material for adsorbing and removing mycotoxins in food, characterized in that: At room temperature and pressure, graphite or a mixture of graphite and ferroferric oxide is ball-milled into solid powder to obtain a graphite-phase carbon material for adsorbing and removing fungal toxins in food.

2. The method for preparing a graphite-phase carbon material for adsorbing and removing fungal toxins in food according to claim 1, characterized in that: The mass ratio of ferrosoferric oxide to graphite in the mixture is (0.01-100):

1.

3. The method for preparing a graphite-phase carbon material for adsorbing and removing fungal toxins in food according to claim 2, characterized in that: The graphite is any one of flake graphite, expanded graphite and amorphous graphite.

4. The method for preparing a graphite-phase carbon material for adsorbing and removing fungal toxins in food according to claim 3, characterized in that: The ball milling speed of the ball milling process is 100-1000 rpm, and the ball milling reaction time is 100-600 min.

5. A graphite phase carbon material for adsorbing and removing mycotoxins in food obtained by the preparation method described in claims 1 to 4.

6. Use of the graphite phase carbon material according to claim 5 in the adsorption and removal of mycotoxins in food.

7. The use according to claim 6, characterized in that: The graphite phase carbon material is dispersed in the food sample and mixed, and then an adsorption reaction is carried out under stirring or shaking. The reaction time is 5 s to 60 min. After the adsorption reaction is completed, the graphite phase carbon material is separated from the food sample.

8. The use according to claim 7, characterized in that: The food is vegetable oil, and the fungal toxin is one or more of aflatoxin, zearalenone, ochratoxin, deoxynivalenol, T-2 toxin, and fumonisin.

9. The use according to claim 8, characterized in that: The vegetable oil is one or more of peanut oil, soybean oil, corn germ oil, rapeseed oil, sesame oil, walnut oil, linseed oil, rice bran oil, wheat germ oil, edible vegetable blended oil, sunflower seed oil, olive oil, camellia seed oil, perilla seed oil, and almond oil.

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