Paeonia suffruticosa collagen polypeptide composition for tightening and resisting wrinkles and preparation method thereof

Abstract

The invention relates to the field of external skin care products, in particular to a peony collagen polypeptide composition for tightening and resisting wrinkles and a preparation method of the peony collagen polypeptide composition. The peony collagen polypeptide composition for tightening and resisting wrinkles is prepared from the following components in percentage by mass: 1 to 3 percent of peony root extract, 10 to 20 percent of polypeptide mixture, peony flower water, 0.3 to 0.6 percent of soluble collagen, 0.1 to 0.5 percent of ganoderma sinensis extract, 0.5 to 2 percent of white truffle fungus extract and 0.5 to 2 percent of lalang grass rhizome extract. The invention has the advantages of enhancing the firming and anti-wrinkle effects, improving the tolerance and the like.

Description

Technical Field

[0001] The present application relates to the field of external skin care products, and more specifically, to a peony collagen polypeptide composition for firming and anti-wrinkle and a preparation method thereof. Background Art

[0002] In the field of beauty and skin care, the demand for anti-aging skin care products is growing, and most of the existing products on the market rely on chemical synthetic ingredients, such as retinoic acid, retinol, etc. These ingredients can indeed bring certain anti-aging effects in the short term, such as reducing fine lines and improving skin firmness. However, long-term use of these chemical synthetic ingredients may burden the skin and cause problems such as dryness, sensitivity, redness and swelling, and even allergic reactions, limiting their safety in long-term application.

[0003] In view of the potential risks of chemical synthetic ingredients, consumers are gradually turning to more natural and gentle skin care products. Natural ingredients, such as plant extracts and natural proteins, have become a hot spot in skin care product research and development in recent years due to their good biocompatibility and low irritation. These ingredients can not only provide anti-aging effects, but also reduce skin irritation and improve the safety and comfort of products.

[0004] Although natural ingredients have broad application prospects in skin care products, existing anti-wrinkle and firming skin care formulas still face many challenges. Due to the complex molecular structure and high molecular weight of plant extracts, it is difficult for them to effectively penetrate deep into the skin, thus limiting their anti-aging effects. Summary of the invention

[0005] In order to enhance the firming and anti-wrinkle effect, the present application provides a peony collagen polypeptide composition for firming and anti-wrinkle and a preparation method thereof.

[0006] In the first aspect, the present application provides a peony collagen polypeptide composition for firming and anti-wrinkle, which adopts the following technical scheme: A peony collagen polypeptide composition for firming and anti-wrinkle comprises the following components by mass percentage: 1-3% peony root extract, 10-20% polypeptide mixture, peony flower water, 0.3-0.6% soluble collagen, 0.1-0.5% Chinese ganoderma lucidum extract, 0.5-2% white truffle extract, and 0.5-2% Imperata root extract.

[0007] Preferably, in terms of mass percentage, the peony root extract is 2%, the white truffle extract is 1%, and the Imperata root extract is 1%.

[0008] By adopting the above technical scheme, peony root extract, white truffle extract and Imperata root extract work together to more effectively penetrate deep into the skin, and improve the permeability and absorption rate of active ingredients through mechanisms such as improving skin barrier function, promoting skin cell renewal and repair, regulating skin metabolism, and antioxidant and anti-inflammatory effects, thereby more effectively improving skin problems.

[0009] Specifically, peony root extract is rich in a variety of active ingredients, such as paeonol, which have a small molecular weight and good skin permeability. The active ingredients in white truffle extract, such as amino acids, minerals and vitamins, have excellent moisturizing and nourishing effects. Active ingredients such as polysaccharides in Imperata root extract have the effect of regulating skin metabolism.

[0010] On this basis, the three work together to enhance the integrity of the skin barrier, reduce water loss, and improve the skin's ability to retain moisture. The improved skin barrier function helps other active ingredients penetrate deeper into the skin, thereby increasing the absorption rate and effectiveness of skin care products. In addition, the three can also synergistically accelerate the metabolism and renewal process of skin cells, thereby improving the permeability of the skin. By promoting the renewal and repair of skin cells, the three extracts can reduce signs of skin aging, such as fine lines and wrinkles, while making the skin firmer and more elastic.

[0011] Moreover, the synergistic cooperation of the three also gives the composition the effect of improving skin tolerance. Specifically, under the synergistic effect, free radicals are removed together to reduce the oxidative damage of skin cells. This synergistic antioxidant effect not only enhances the antioxidant capacity of the skin, but also promotes the health and vitality of skin cells. At the same time, the anti-inflammatory components in them can also complement each other to form a powerful anti-inflammatory barrier, effectively reduce the skin's inflammatory response, relieve discomfort symptoms such as redness, swelling, and pain, and make the skin more stable and healthy. And effectively enhance the skin's resistance to external stimuli, inhibit the reproduction of bad bacteria on the skin, and reduce skin problems caused by bacterial infection. Provide a solid protective barrier for the skin and reduce the occurrence of skin problems. At the same time, the natural moisturizing factors and nourishing ingredients they are rich in can enhance the moisturizing barrier function of the skin, reduce water loss, and keep the skin soft and smooth. This moisturizing and nourishing effect not only improves the problem of dry and tight skin, but also improves the comfort and tolerance of the skin.

[0012] Preferably, the polypeptide mixture comprises at least one or more of acetyl hexapeptide-8, dipeptide-2, oligopeptide-1, and palmitoyl tetrapeptide-7.

[0013] Preferably, the polypeptide mixture includes acetyl hexapeptide-8, dipeptide-2, oligopeptide-1, and palmitoyl tetrapeptide-7; according to the mass percentage of the polypeptide mixture, acetyl hexapeptide-8 is 0.05-0.15%, dipeptide-2 is 0.01-0.05%, oligopeptide-1 is 0.008-0.02%, and palmitoyl tetrapeptide-7 is 0.008-0.02%.

[0014] By adopting the above technical solution, the active ingredients in the polypeptide mixture are optimized, so that the active ingredients complement each other and enhance their synergy.

[0015] As a neurotransmitter inhibitory peptide, acetyl hexapeptide-8 effectively reduces the frequency of muscle contraction by inhibiting the release of neurotransmitters, thereby avoiding wrinkles caused by excessive muscle contraction. At the same time, it can also activate collagen activity and increase the activity of elastin, providing dual support for skin firmness and elasticity.

[0016] Dipeptide-2 improves the blood circulation and lymphatic circulation of the skin by inhibiting angiotensin-converting enzyme, helps to remove toxins and edema in the skin, and further improves the firmness and smoothness of the skin. In addition, dipeptide-2 can also directly act on skin cells, promote cell growth and repair, and enhance the skin's self-repair ability.

[0017] Oligopeptide-1, with its easy absorption by the skin, participates in the extension of peptide chains as a raw material, improving the efficiency of protein synthesis. This not only promotes the growth and division of skin cells, but also enhances the skin's ability to moisturize and replenish water, making the skin more hydrated and smooth. At the same time, oligopeptide-1 can also improve the skin's repair ability, help improve skin problems such as acne scars, and make the skin healthier and firmer.

[0018] Palmitoyl Tetrapeptide-7 significantly improves the firmness and elasticity of the skin by promoting the synthesis of extracellular matrix such as collagen in the dermis. It can also inhibit inflammatory reactions and glycosylation damage, and reduce skin aging problems caused by inflammation and pigmentation. In addition, Palmitoyl Tetrapeptide-7 can also increase the water content and moisture retention of the skin, making the skin more hydrated and smooth.

[0019] The specific active ingredients in these peptide blends not only work in synergy, but also interact and synergize to improve skin firmness and reduce the formation of wrinkles.

[0020] Preferably, the peony root extract is combined with water, alcohol solvent A and antioxidant A to form a peony root mixture; based on the mass percentage of the peony root mixture, the peony root extract is 8-15%, the alcohol solvent A is 15-25%, and the antioxidant A is 0.1-1%.

[0021] Preferably, the white truffle extract is combined with water, alcohol solvent B and antioxidant B to form a white truffle mixture; in terms of mass percentage of the white truffle mixture, the white truffle extract is 8-15%, the alcohol solvent B is 8-15%, and the antioxidant B is 0.1-1%.

[0022] Preferably, the Imperata root extract is combined with water, alcohol solvent C and antioxidant C to form an Imperata root mixture; based on the mass percentage of the Imperata root mixture, the white truffle extract is 8-15%, the alcohol solvent C is 8-15%, and the antioxidant C is 0.1-1%.

[0023] By adopting the above technical scheme, peony root extract, white truffle extract and Imperata root extract are involved in the peony collagen polypeptide composition for firming and anti-wrinkle in the form of peony root mixture, white truffle mixture and Imperata root mixture, which can effectively prolong the action period of the active substances and also enable the active substances to be more fully mixed and evenly exerted in the peony collagen polypeptide composition system for firming and anti-wrinkle.

[0024] In a second aspect, the present application provides a method for preparing a peony collagen polypeptide composition for firming and anti-wrinkle, using the following technical solution: A method for preparing a peony collagen polypeptide composition for firming and anti-wrinkle comprises the following steps: all raw materials are mixed until uniform to obtain the peony collagen polypeptide composition for firming and anti-wrinkle.

[0025] 1. Since the present application adopts peony root extract, white truffle extract and Imperata root extract, the three work together to more effectively penetrate deep into the skin, and improve the permeability and absorption rate of active ingredients through mechanisms such as improving skin barrier function, promoting skin cell renewal and repair, regulating skin metabolism, and antioxidant and anti-inflammatory effects, thereby more effectively improving skin problems.

[0026] 2. The synergistic combination of peony root extract, white truffle extract and Imperata root extract significantly enhances the ability of the composition to improve skin tolerance, effectively enhances the skin's resistance to external stimuli, and builds a solid protective barrier for the skin. At the same time, the rich natural moisturizing and nourishing ingredients strengthen the skin's moisturizing barrier, reduce water loss, and make the skin soft and smooth, which not only solves the problem of dryness and tightness, but also improves the skin's comfort and overall tolerance. DETAILED DESCRIPTION

[0027] The present application is further described in detail below with reference to the embodiments.

[0028] The raw materials used in the following examples and comparative examples are all commercially available products. Example

[0029] Example 1 A peony collagen polypeptide composition for firming and anti-wrinkle comprises a peony root mixture, a polypeptide mixture, a peony flower water mixture, a soluble collagen mixture, a Chinese ganoderma mixture, a white truffle mixture, and an Imperata root mixture.

[0030] The specific amounts of various raw materials are shown in Table 1.

[0031] The peony root mixture was purchased from Guangzhou Fuzhilin Biotechnology Co., Ltd., the polypeptide mixture was purchased from Shenzhen Mingkang Biotechnology Co., Ltd., the peony flower water mixture was purchased from Guangzhou Fuzhilin Biotechnology Co., Ltd., the soluble collagen mixture was purchased from Jiangsu Jiangshan Juyuan Biotechnology Co., Ltd., the Chinese Ganoderma lucidum mixture was purchased from Guangzhou Fuzhilin Biotechnology Co., Ltd., and the white truffle mixture and Imperata root mixture were purchased from Guangzhou Yaozhi Biotechnology Co., Ltd.

[0032] The effective content of peony root extract in the peony root mixture is 10%. The peony root mixture is composed of 68.5% water, 20% butylene glycol (alcohol solvent A), 10% peony root extract, 0.5% p-hydroxyacetophenone (antioxidant A), and 1% 1,2-hexanediol (alcohol solvent A).

[0033] The polypeptide mixture is composed of 50% glycerin, 48.54% water, 1% 1,2-hexanediol, 0.2% p-hydroxyacetophenone, 0.1% sodium hyaluronate, 0.1% acetyl hexapeptide-8, 0.03% dipeptide-2, 0.015% oligopeptide-1, and 0.015% palmitoyl tetrapeptide-7.

[0034] The peony flower water mixture is composed of 99% peony (PAEONIA SUFFRUTICOSA) flower water, 0.5% p-hydroxyacetophenone, and 0.5% 1,2-hexanediol.

[0035] The soluble collagen mixture is compounded by 80.47% water, 10% glycerol, 3.2% soluble collagen, 3% phosphatidylcholine, 2% 1,2-pentanediol, 0.65% cetyl alcohol, 0.5% 1,2-hexanediol, 0.1% sodium chloride and 0.08% xanthan gum.

[0036] The Chinese Ganoderma lucidum mixture is compounded by 97.3% water, 2% Chinese Ganoderma lucidum (GANODERMA SINENSIS) extract, 0.63% phenoxyethanol, and 0.07% ethylhexylglycerin.

[0037] The effective content of white truffle (TUBER MAGNATUM) extract in the white truffle mixture is 10%. The white truffle mixture is compounded by 79% water, 10% butanediol (alcohol solvent B), 10% white truffle (TUBER MAGNATUM) extract, 0.5% 1,2-hexanediol (alcohol solvent B), and 0.5% p-hydroxyacetophenone (antioxidant B).

[0038] The effective content of Imperata cylindrica major root extract in the Imperata cylindrica major root mixture is 10%. The Imperata cylindrica major root mixture is compounded by 79% water, 10% butylene glycol (alcohol solvent C), 10% Imperata cylindrica major root extract, 0.5% 1,2-hexanediol (alcohol solvent C), and 0.5% p-hydroxyacetophenone (antioxidant C).

[0039] The present application also provides a method for preparing a peony collagen polypeptide composition for firming and anti-wrinkle, comprising the following steps: mixing various raw materials until uniform to obtain a peony collagen polypeptide composition for firming and anti-wrinkle.

[0040] Example 2-3 A peony collagen polypeptide composition for firming and anti-wrinkle, which is different from Example 1 in that the specific content of each raw material is different, see Table 1 for details.

[0041] See Table 1 for details.

[0042] Table 1 Comparative Example Comparative Example 1 A peony collagen polypeptide composition for firming and anti-wrinkle, which is different from Example 1 in that the peony root extract is replaced by a licorice extract, that is, the peony root mixture is changed into a licorice mixture.

[0043] Licorice extract was commercially available (10:1) from Shanyang Lianfeng Biotechnology Co., Ltd.

[0044] Specifically, 10% licorice extract (powder) was mixed with 68.5% water, 20% butylene glycol (alcohol solvent A), 0.5% p-hydroxyacetophenone (antioxidant A), and 1% 1,2-hexanediol (alcohol solvent A) until uniform, to obtain a licorice mixture.

[0045] Comparative Example 2 A peony collagen polypeptide composition for firming and anti-wrinkle, which is different from Example 1 in that the white truffle extract is replaced by the Centella asiatica extract, that is, the white truffle mixture is changed into the Centella asiatica mixture.

[0046] Centella asiatica extract was commercially available (10:1) from Shaanxi Junhe Biotechnology Co., Ltd.

[0047] Specifically, 10% of Centella asiatica extract (powder) is mixed with 79% of water, 10% of butylene glycol (alcohol solvent B), 0.5% of p-hydroxyacetophenone (antioxidant B), and 0.5% of 1,2-hexanediol (alcohol solvent B) until uniform, to obtain a Centella asiatica mixture.

[0048] Comparative Example 3 A peony collagen polypeptide composition for firming and anti-wrinkle, which is different from Example 1 in that the Imperata root extract is replaced by resveratrol extract, that is, the Imperata root mixture is changed into a resveratrol mixture.

[0049] Resveratrol extract was commercially available (10:1) from Guangzhou Huayu Biotechnology Co., Ltd.

[0050] Specifically, 10% resveratrol extract (powder) was mixed with 79% water, 10% butylene glycol (alcohol solvent C), 0.5% p-hydroxyacetophenone (antioxidant C), and 0.5% 1,2-hexanediol (alcohol solvent C) until uniform, to obtain a resveratrol mixture.

[0051] Comparative Example 4 A peony collagen polypeptide composition for firming and anti-wrinkle, which is different from Example 1 in that the polypeptide mixture is omitted and the content of the original polypeptide mixture is supplemented with a peony flower water mixture.

[0052] Performance testing 1. Preparation of test samples: A facial cream comprises the following components by mass percentage: water, 10% glycerol, 4.92865% propylene glycol, 3% mineral oil, 0.25% sodium poly-gamma-glutamate, 0.01% phenoxyethanol, 0.5% ammonium acryloyldimethyltaurate / VP copolymer, 0.12% bis(hydroxymethyl)imidazolidinyl urea, 0.00135% iodopropynyl butylcarbamate, 0.2% carbomer, 0.2% triethanolamine, 0.15% methylparaben, and 13% peony collagen polypeptide composition for firming and anti-wrinkle.

[0053] The preparation method of the facial cream comprises the following steps: Step a: Add water, glycerin, propylene glycol, ammonium acryloyldimethyltaurate / VP copolymer, carbomer, and methylparaben into an emulsifier, homogenize for 15 minutes, heat to 85°C, and keep warm for 20 minutes.

[0054] Step b: Cool down to 75°C, add mineral oil into the emulsifier, and homogenize under vacuum for 3 minutes.

[0055] Step c: cool down to 40° C., put sodium poly-γ-glutamate, phenoxyethanol, bis(hydroxymethyl)imidazolidinyl urea, iodopropynyl butylcarbamate, triethanolamine, and the peony collagen polypeptide composition for firming and anti-wrinkle into an emulsifying pot in sequence, and stir evenly.

[0056] Step d: The material of step c is aged for 24 hours to obtain a facial cream.

[0057] 2. Volunteer selection: 35 volunteers aged 30-40 were selected and randomly divided into 7 groups, with 5 people in each group. One group of volunteers corresponded to a cream made from the composition of Examples 1-3 and Comparative Examples 1-4. The volunteer exclusion criteria met the exclusion criteria in the Diagnostic Criteria and Treatment Principles of Cosmetic Contact Dermatitis.

[0058] 3. Test items: 1. Skin elasticity test: Volunteers apply facial cream to the test area once a day in the morning and evening for 28 consecutive days. During this period, no other cosmetics can be applied to the test area.

[0059] A skin elasticity meter was used to collect data from the test sites of the volunteers on the 0th day and after the 28th day. The same test area was measured 3 times and the average value was taken.

[0060] The skin elasticity index is characterized by R2, which refers to the rebound amount of the skin when there is no negative pressure. a Maximum stretching under negative pressure U f The closer R2 is to 1, the better the skin elasticity.

[0061] The elasticity change rate was calculated according to the following formula, and the average value of the elasticity change rate of the volunteers in the same group was taken as the result of the group and recorded in Table 2.

[0062] At the same time, the volunteers were asked to rate the firming and anti-wrinkle effects on a scale of 0-10. The closer to 0, the less obvious the firming and anti-wrinkle effects; the closer to 10, the more obvious the firming and anti-wrinkle effects. The average of the volunteers in the same group was taken as the group result and recorded in Table 2.

[0063] Table 2 According to the test data in Table 2, the creams prepared in Examples 1-3 can make the skin more elastic, and users can also subjectively feel the good firming and anti-wrinkle effect. Although the R2 value of the creams in Comparative Examples 1-3 is larger and closer to 1 on the 28th day than the R2 value on the 0th day in the skin elasticity test, that is, the creams in Comparative Examples 1-3 can also improve the elasticity of the skin. However, according to the test data of Examples 1-3 and Comparative Examples 1-3 in Table 2, it can be seen that the elasticity change rate of Comparative Examples 1-3 is obviously lower than that of Example 1-3, indicating that the improvement effect of Comparative Examples 1-3 on the skin is not as significant as that of Example 1-3.

[0064] That is to say, the peony collagen polypeptide composition for firming and anti-wrinkle of Comparative Examples 1-3 is based on Example 1, and any of the peony root extract, white truffle extract and Imperata root extract is changed to other plant extracts. After the special combination of peony root extract, white truffle extract and Imperata root extract is destroyed, the synergistic cooperation of the three cannot be achieved, the permeability and absorption rate of the active ingredients are poor, and the firming and anti-wrinkle lifting effect on the skin is not obvious.

[0065] Comparative Example 4 is based on Example 1, but the polypeptide mixture is replaced with a peony flower water mixture. The performance of Comparative Example 4 is somewhat lower than that of Example 1, but is still better than that of Comparative Examples 1 to 3. This shows that the peony root extract, the white truffle extract and the Imperata root extract do have a good synergistic effect, and on this basis, the use of the polypeptide mixture can further improve the firming and anti-wrinkle effect.

[0066] 2. Tolerance test: Drop 1 mL of 2% histamine solution onto the arm surface, observe the test area 30 minutes later, and score the degree of redness and stinging. And record the number of people whose test area is still red and swollen 3 hours after histamine stimulation. This is the initial test result, recorded in Table 3.

[0067] Then, the volunteers were asked to apply the cream to the test area once a day in the morning and evening for 28 consecutive days. During this period, no other cosmetics could be applied to the test area. After 28 days, the above stimulation operation was repeated again, and the degree of redness, stinging sensation, and the number of people who were still red and swollen in the test area 3 hours after histamine stimulation were recorded in Table 3. This is the 28-day test result.

[0068] The redness and stinging sensation were scored on a scale of 0-5. The closer to 0, the less redness / slight stinging sensation; the closer to 5, the more redness / severe stinging sensation. The average value of the volunteers in the same group was taken as the data for this group.

[0069] Table 3 According to the test results in Table 3, the volunteers who used the creams of Examples 1-3 had significantly improved redness and stinging sensation after being stimulated for 28 days compared with the initial stage, and the redness and swelling of the tested area basically disappeared after 3 hours of histamine stimulation, indicating that the products of Examples 1-3 can improve the skin tolerance.

[0070] However, the volunteers who used the cream of Comparative Examples 1-3 for 28 days did not show much improvement in redness and stinging sensation. After 3 hours of histamine stimulation, the tested area still showed obvious redness and swelling, indicating that Comparative Examples 1-3 did not significantly improve tolerance.

[0071] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed, but such modifications are protected by the patent law as long as they are within the scope of the claims of the present application.

Claims

1. A peony collagen polypeptide composition for firming and anti-wrinkle, characterized in that: According to mass percentage, it includes the following components: 1-3% peony root extract, 10-20% polypeptide mixture, peony flower water, 0.3-0.6% soluble collagen, 0.1-0.5% Chinese ganoderma lucidum extract, 0.5-2% white truffle extract, and 0.5-2% Imperata root extract.

2. The peony collagen polypeptide composition for firming and anti-wrinkle according to claim 1, characterized in that: In terms of mass percentage, the peony root extract is 2%, the white truffle extract is 1%, and the Imperata root extract is 1%.

3. The peony collagen polypeptide composition for firming and anti-wrinkle according to claim 1, characterized in that: The polypeptide mixture at least includes a mixture of one or more of acetyl hexapeptide-8, dipeptide-2, oligopeptide-1, and palmitoyl tetrapeptide-7.

4. The peony collagen polypeptide composition for firming and anti-wrinkle according to claim 3, characterized in that: The polypeptide mixture includes acetyl hexapeptide-8, dipeptide-2, oligopeptide-1, and palmitoyl tetrapeptide-7; according to the mass percentage of the polypeptide mixture, acetyl hexapeptide-8 is 0.05-0.15%, dipeptide-2 is 0.01-0.05%, oligopeptide-1 is 0.008-0.02%, and palmitoyl tetrapeptide-7 is 0.008-0.02%.

5. The peony collagen polypeptide composition for firming and anti-wrinkle according to claim 1, characterized in that: The peony root extract is combined with water, alcohol solvent A and antioxidant A to form a peony root mixture; based on the mass percentage of the peony root mixture, the peony root extract is 8-15%, the alcohol solvent A is 15-25%, and the antioxidant A is 0.1-1%.

6. The peony collagen polypeptide composition for firming and anti-wrinkle according to claim 1, characterized in that: The white truffle extract is combined with water, alcohol solvent B and antioxidant B to form a white truffle mixture; in terms of mass percentage of the white truffle mixture, the white truffle extract is 8-15%, the alcohol solvent B is 8-15%, and the antioxidant B is 0.1-1%.

7. The peony collagen polypeptide composition for firming and anti-wrinkle according to claim 1, characterized in that: The Imperata root extract is combined with water, alcohol solvent C and antioxidant C to form an Imperata root mixture; based on the mass percentage of the Imperata root mixture, the white truffle extract is 8-15%, the alcohol solvent C is 8-15%, and the antioxidant C is 0.1-1%.

8. A method for preparing the peony collagen polypeptide composition for firming and anti-wrinkle according to any one of claims 1 to 7, characterized in that: The following steps are involved: All raw materials are mixed evenly to obtain a peony collagen polypeptide composition for firming and anti-wrinkle.

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