Plant essential oil composition and application thereof in preparation of medicament for inhibiting activity of mould

Abstract

The invention relates to the technical field of cultural relic protection, and particularly discloses a plant essential oil composition and application thereof in preparation of a medicament for inhibiting mould activity. The plant essential oil composition is prepared from the following raw materials: dementholized peppermint oil and cinnamon oil; the volume ratio of the dementholized peppermint oil to the cinnamon oil is (64: 1)-(16: 1). When the dementholized peppermint oil and the cinnamon oil are combined for use, the dementholized peppermint oil and the cinnamon oil can show a synergistic effect of inhibiting mould in air, and the main reason is that the dementholized peppermint oil and the cinnamon oil have different functions, so that the antibacterial effect can be enhanced through different action mechanisms; besides, the use of the plant essential oil composition can reduce the use amount of single essential oil, so that the potential toxicity and side effects can be reduced, and meanwhile, the risk that bacteria generate drug resistance to the single essential oil can be reduced.

Description

Technical Field

[0001] The invention relates to the technical field of cultural relics protection, and in particular to a plant essential oil composition and application thereof in preparing a medicament for inhibiting mold activity. Background Art

[0002] Although the current conditions for the protection and display of cultural relics in museums have been significantly improved, the problem of cultural relics being damaged by mold is still very common; therefore, the development of agents that are both harmless to cultural relics and can prevent mold has always been a key and valuable research direction in cultural relic conservation.

[0003] Among the many methods for preventing and controlling mildew in museum artifacts, fumigation is recognized as an extremely effective and thorough means. Commonly used fumigants include ethylene oxide, methyl bromide and sulfuryl fluoride, etc. The anti-mildew effects of these agents in many industries such as washing, pharmaceuticals, and food have been verified; however, the safety issues of these fumigants have also caused widespread discussion. For example, ethylene oxide is flammable and explosive and has the risk of potential carcinogenesis, methyl bromide may cause damage to the environment and be toxic to the human nervous system, and sulfuryl fluoride may have adverse effects on the atmosphere; compared with these chemical fumigants, plant essential oils, as a natural antibacterial agent, not only have strong antibacterial ability, but are also safer for the human body and have less impact on the environment, so their application is becoming more and more widespread.

[0004] In the field of cultural relics protection, the potential of essential oils is gradually being explored and utilized; peppermint oil and cinnamon oil are both essential oils with significant antibacterial effects. Peppermint oil is a volatile oil obtained from the fresh stems and leaves of mint by steam distillation. It has a special cool aroma. Its main components include menthol, menthone, etc., which have a certain inhibitory effect on a variety of bacteria and fungi; peppermint oil has a good antibacterial effect on enteric pathogens such as Escherichia coli, especially peppermint oil, which also has significant antibacterial activity against multi-drug resistant strains. Cinnamon oil is an oily liquid extracted from the dry bark, cinnamon twigs, cinnamon leaves and other parts of cinnamon. The main component is cinnamaldehyde, which has a strong spicy aroma and aromatic smell; cinnamon oil shows effective antibacterial activity against foodborne spoilage bacteria and pathogens such as Escherichia coli and Staphylococcus aureus; the antibacterial mechanism of cinnamon oil is mainly to destroy the integrity of the bacterial cell wall membrane, affect the permeability of the cell membrane, and cause the leakage of cell contents, thereby inhibiting the growth and reproduction of bacteria.

[0005] There are currently no studies investigating the use of peppermint oil and cinnamon oil together as an antifungal agent. Summary of the invention

[0006] In view of this, the object of the present invention is to provide a plant essential oil composition and its use in preparing a medicament for inhibiting mold activity.

[0007] To achieve the above object, on the one hand, the present invention provides a plant essential oil composition, comprising the following preparation raw materials: peppermint oil and cinnamon oil.

[0008] As a further improvement to the above technical solution, the volume ratio of the peppermint oil to the cinnamon oil is 64:1 to 16:1.

[0009] As a further improvement to the above technical solution, the peppermint oil contains 23.5-37.5% of menthone, 24.0-38.5% of menthol and 0-6.00% of menthyl acetate.

[0010] As a further improvement to the above technical solution, the cinnamon oil contains 18.0-35.0% of limonene, 10.0-25.0% of cinnamaldehyde and 10.0-15.0% of linalool.

[0011] On the other hand, the present invention also provides the use of the plant essential oil composition in the preparation of a medicament for inhibiting fungal activity.

[0012] As a further improvement to the above technical solution, the mold includes Aspergillus niger, Penicillium citrinum and / or Trichoderma longipedulum.

[0013] As a further improvement to the above technical solution, the mold is Aspergillus niger, and the volume ratio of peppermint oil to cinnamon oil is 64:1 to 32:1.

[0014] As a further improvement to the above technical solution, the mold is Penicillium citrinum, and the volume ratio of peppermint oil to cinnamon oil is 64:1 to 16:1.

[0015] As a further improvement to the above technical solution, the mold is Trichoderma longiflorum, and the volume ratio of peppermint oil to cinnamon oil is 16:1.

[0016] As a further improvement to the above technical solution, the medicament is a fumigation essential oil used in conjunction with a fumigator, and the fumigation essential oil is formed by mixing peppermint oil and cinnamon oil in a set volume ratio.

[0017] Compared with the prior art, the present invention has the following beneficial technical effects:

[0018] The present invention provides a plant essential oil composition. Experiments show that when peppermint oil and cinnamon oil are used in combination, they can show a synergistic effect of inhibiting mold in the air. The main reason is that the effects and functions of peppermint oil and cinnamon oil are different, and the antibacterial effect can be enhanced through different mechanisms of action. In addition, the use of the plant essential oil composition can also reduce the amount of a single essential oil, thereby reducing potential toxicity and side effects, and also reduce the risk of bacteria developing resistance to a single essential oil, and is particularly suitable for application in the field of museum cultural relics protection.

[0019] The plant essential oil composition of the present invention can be used as or to prepare a medicament for inhibiting mold activity. The prepared medicament also has the above-mentioned technical effects, which is conducive to the development of a medicament that is harmless to cultural relics and can prevent mold, thereby solving the problem of cultural relics being damaged by mold.

[0020] Advantages of additional aspects of the present invention will be given in part in the following description, and in part will become obvious from the following description, or will be learned through practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 This is a graph showing the growth of Aspergillus niger after 48 hours of combined antibacterial experiment;

[0022] Figure 2 This is a graph showing the growth of Penicillium citrinum in the combined antibacterial experiment for 48 hours;

[0023] Figure 3 This is a graph showing the growth of Trichoderma longipes after 48 hours of combined antibacterial experiment. DETAILED DESCRIPTION

[0024] In order to enable those skilled in the art to better understand the technical solution of the present invention, the present invention is further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0025] Embodiment 1

[0026] 1. Experiment on the sensitivity of different molds to peppermint oil and cinnamon oil

[0027] 1.1 Experimental equipment and reagents:

[0028] 1.1.1 Main reagents for the experiment: Sabouraud dextrose solid medium, purchased from Qingdao Haibo Biotechnology Co., Ltd.; Sabouraud nutrient agar medium, purchased from Chongqing Pangtong Medical Equipment Co., Ltd.

[0029] 1.1.2 Tested molds: As shown in Table 1, all are from the State Administration of Cultural Heritage Key Scientific Research Base for Pest Control of Museum Cultural Relics (China Three Gorges Museum, Chongqing).

[0030] Table 1 Information of tested molds

[0031]

[0032] 1.1.3 Test essential oils: cinnamon oil and peppermint oil, both purchased from Chongqing Daily Chemical Industry Research Institute.

[0033] 1.2 Experimental methods:

[0034] 1.2.1 Spore liquid preparation

[0035] Elute the freshly cultured mold plate with sterile deionized water and transfer the suspension to a sterilized reagent bottle. Dilute the spore stock solution ten-fold into multiple gradients and determine the concentration of each gradient spore suspension by plate counting. Select a concentration of 1.0x10 6 ~5.0x10 6 mL -1 The spore liquid is used as the spore liquid for making bacterial plates.

[0036] 1.2.2 Preparation of drug sensitivity paper

[0037] Use a hole puncher to punch out circular filter paper pieces with a diameter of 6 mm on the qualitative filter paper, and sterilize them in a steam sterilizer at 121°C for 15 minutes.

[0038] 1.2.3 Preparation of bacterial plates

[0039] After mixing the prepared spore suspension evenly, transfer it to a disposable sterile culture dish (Φ70mm) with a pipette, 1mL per dish. After preparing the Sabouraud dextrose solid medium according to the instructions, sterilize it in a steam sterilizer at 121℃ for 15min, cool it to about 50℃, pour it into the culture dish, 10-15mL per dish, and gently shake the culture dish to mix it with the bacterial solution.

[0040] 1.2.4 Antibacterial activity assay

[0041] Use sterile tweezers to stick a drug-sensitive paper sheet in the center of the solidified bacterial plate. Pipette 5uL of sterilized ultrapure water on the paper sheet of the control plate, and pipette 5uL of each test essential oil on the filter paper sheet of the experimental group. Repeat each treatment 3 times. After the plate is sealed with a sealing film, it is cultured in an incubator under the following conditions: temperature 28°C, relative humidity 80%. After 48 hours, the diameter of the inhibition zone is measured by the cross-cross method using a vernier caliper, and the growth of different molds on the bacterial plate is observed.

[0042] 1.2.5 Sensitivity judgment criteria

[0043] According to the conventional antibiotic susceptibility test inhibition zone sensitivity judgment standard, the inhibition zone diameter greater than 20mm is extremely sensitive, 15-20mm is highly sensitive, 10-15mm is moderately sensitive, and 6-10mm is low sensitivity; when the inhibition zone diameter is less than 6mm of the filter paper diameter, there is no antibacterial effect.

[0044] 1.3 Experimental Results

[0045] 1.3.1 Sensitivity of Aspergillus niger to the tested essential oils

[0046] Table 2 Sensitivity of Aspergillus niger to the tested essential oils

[0047]

[0048] 1.3.2 Sensitivity of Penicillium citrinum to the tested essential oils

[0049] Table 3 Sensitivity of Penicillium citrinum to the tested essential oils

[0050]

[0051] 1.3.3 Sensitivity of Trichoderma longipes to the tested essential oils

[0052] Table 4 Sensitivity of Trichoderma longipes to the tested essential oils

[0053]

[0054] 1.3.4 Summary

[0055] Aspergillus, Penicillium and Trichoderma are all common contaminating bacteria in cultural relics and the preservation environment of cultural relics. In this experiment, peppermint oil and cinnamon oil were used to test the sensitivity of three contaminating bacteria (Aspergillus niger, Penicillium citrinum and Trichoderma longipes) collected from the surface of cultural relics and the preservation environment. The results showed that the three common contaminating bacteria of cultural relics were extremely sensitive to cinnamon oil; Aspergillus niger and Penicillium citrinum were moderately sensitive to peppermint oil, and Trichoderma longipes was extremely sensitive to peppermint oil. Cinnamon oil has a strong inhibitory effect on the three common contaminating bacteria in cultural relics and the preservation environment of cultural relics, while peppermint oil has a weaker inhibitory effect on Aspergillus niger and Penicillium citrinum, but can slow down their growth rate.

[0056] 2. Minimum inhibitory concentration (MIC) of peppermint oil and cinnamon oil against three fungi

[0057] 2.1 Experimental equipment and reagents:

[0058] 2.1.1 Main reagents for the experiment:

[0059] Sabouraud dextrose agar medium was purchased from Qingdao Haibo Biotechnology Co., Ltd.; Tween 80 was purchased from Sinopharm Chemical Reagent Co., Ltd.

[0060] 2.1.2 Tested fungi: Same as 1.1.2 above

[0061] 2.1.3 Test essential oil: same as 1.1.3 above

[0062] 2.2 Experimental methods:

[0063] 2.2.1 Spore liquid preparation: same as 1.2.1 above, select 1.0x10 5 ~5.0x10 5 mL -1 The spore fluid was used as spore fluid for subsequent tests.

[0064] 2.2.2 Preparation of plates containing different concentration gradients of essential oils

[0065] Tween 80 was prepared as a 5% solution, and Sabouraud glucose agar medium was prepared according to the instructions. Tween 80 solution and the prepared culture medium were sterilized at 121°C in a steam sterilizer for 15 minutes and then used. Tween 80 solution was used to dissolve and dilute the two essential oils in multiple ratios, and a uniform emulsion containing plant essential oils was prepared. The emulsion was mixed with the sterilized culture medium in a ratio of 1:9 to prepare a drug-containing culture medium, which was poured into a culture dish, 10 to 15 mL per dish, and used after cooling and solidification. The final drug-containing culture medium contained 0.5% Tween 80 (w / v), and the content of plant essential oils was 4uL / mL, 2uL / mL, 1uL / mL, 0.5uL / mL, 0.25uL / mL, 0.125uL / mL, 0.0625uL / mL, and 0.03125uL / mL, respectively. The control group culture medium only contained 0.5% Tween 80.

[0066] 2.2.2 MIC determination of peppermint oil and cinnamon oil against three fungi

[0067] Take 100uL of spore liquid (prepared in 2.2.1 above) and evenly spread the spore liquid on the surface of the plate containing plant essential oil (prepared in 2.2.2 above) using the plate spreading method. Repeat three times for each group. Place the inoculated plate in an incubator at 28°C and 80% relative humidity. Observe and record the fungal growth on different plates for 48 hours.

[0068] 2.3 Experimental Results

[0069] 2.3.1 Growth in different gradient drug-containing medium for 48 h

[0070] Table 5 Fungal growth on essential oil-containing plates for 48 hours

[0071]

[0072]

[0073] Note: “+” indicates mold growth, “—” indicates no mold growth.

[0074] 2.3 Summary

[0075] The experimental results showed that the minimum inhibitory concentration (MIC) of peppermint oil against Aspergillus niger and Penicillium citrinum was 4uL / mL, and the minimum inhibitory concentration (MIC) against Trichoderma longibrachiatum was 2uL / mL; the minimum inhibitory concentration (MIC) of cinnamon oil against Aspergillus niger, Penicillium citrinum and Trichoderma longibrachiatum was 0.125uL / mL.

[0076] 3. Antibacterial experiment of peppermint oil and cinnamon oil

[0077] 3.1 Experimental equipment and reagents:

[0078] 3.1.1 Main reagents for the experiment:

[0079] Sabouraud dextrose agar medium was purchased from Qingdao Haibo Biotechnology Co., Ltd.; Tween 80 was purchased from Sinopharm Chemical Reagent Co., Ltd.

[0080] 3.1.2 Tested fungi: Same as 1.1.2 above

[0081] 3.1.3 Test essential oil: same as 1.1.3 above

[0082] 3.2 Experimental methods:

[0083] 3.2.1 Spore liquid preparation: same as 1.2.1 above, select 1.0x10 5 ~5.0x10 5 mL -1 The spore fluid was used as spore fluid for subsequent tests.

[0084] 3.2.2 Combined drug sensitivity test of peppermint oil and cinnamon oil (agar dilution checkerboard method)

[0085] Tween 80 was prepared as a 5% solution, and Sabouraud glucose agar medium was prepared according to the instructions. Tween 80 solution and prepared culture medium were sterilized at 121°C in a steam sterilizer for 15 minutes and then used. Tween 80 solution was used to dissolve and dilute cinnamon oil and peppermint oil respectively, and prepared into different concentration gradients of drug solutions, and the prepared drug solutions were mixed with the sterilized culture medium in proportion to prepare a culture medium containing two essential oils in a series of multiple dilutions (see the schematic diagram for drug concentration), and poured into the culture dish, 10-15 mL per dish, and used after cooling and solidification.

[0086] Table 6 Schematic diagram of drug concentration in culture medium

[0087]

[0088] Among them, peppermint oil: A: MIC; B: C: D: E:

[0089] Cinnamon oil: a: MIC; b: c: d: e:

[0090] Take 100uL of spore liquid (prepared in 3.2.1 above) and evenly spread the spore liquid on the surface of the plate containing plant essential oil (prepared in 3.2.2 above) using the plate spreading method. Repeat three times for each group. Place the inoculated plate in an incubator at 28°C and 80% relative humidity. Observe and record the fungal growth on different plates for 48 hours.

[0091] 3.2.3 Evaluation of combined drug sensitivity test

[0092] The fractional inhibitory concentration index (FICI) was used as the evaluation basis for the results of combined drug sensitivity test.

[0093]

[0094] The evaluation basis of the combined drug sensitivity test is: FICI≤0.5, the two have a synergistic effect; 0.5<FICI≤1, the two have an additive effect; 1<FICI≤2, the two have an unrelated effect; FICI>2, the two have an antagonistic effect.

[0095] 3.3 Experimental results:

[0096] 3.3.1 Growth of Aspergillus niger after 48 hours of combined use of cinnamon oil and peppermint oil Figure 1 As shown, it is specially organized in the following Table 7.

[0097] Table 7 Growth of Aspergillus niger after 48h combined use of cinnamon oil and peppermint oil

[0098]

[0099] Note: “+” indicates fungal growth, “—” indicates no fungal growth, and * indicates the MIC concentration of a single essential oil against the tested fungus (the same below).

[0100] 3.3.2 Growth of Penicillium citrinum after 48 hours of combined use of cinnamon oil and peppermint oil Figure 2 As shown, it is specially organized in the following Table 8.

[0101] Table 8 Growth of Penicillium citrinum after combined use of cinnamon oil and peppermint oil for 48 h

[0102]

[0103] 3.3.3 Growth of Trichoderma longipes after 48h combined use of cinnamon oil and peppermint oil Figure 3 As shown, it is specially organized in the following Table 9.

[0104] Table 9 Growth of Trichoderma longipes after combined use of cinnamon oil and peppermint oil for 48 h

[0105]

[0106]

[0107] 3.3.4 Evaluation of the combined drug sensitivity test of cinnamon oil and peppermint oil

[0108] Table 10 Evaluation of the combined drug sensitivity test of cinnamon oil and peppermint oil

[0109]

[0110] 3.3.5 Summary

[0111] The combined use of peppermint oil and cinnamon oil has an additive effect on the inhibition of Trichoderma longibrachiatum, and a synergistic effect on the inhibition of Aspergillus niger and Penicillium citrinum.

[0112] The main reasons for the synergistic effect are: the antibacterial mechanism of cinnamon oil is mainly its main components limonene, cinnamaldehyde and linalool, all of which can destroy the integrity and permeability of microbial cell membranes, affect the metabolic activity and carbon source utilization ability of microorganisms, and change the microscopic structure morphology of microorganisms, thereby achieving the effect of inhibiting and killing mold; the antibacterial mechanism of peppermint oil is mainly its main component menthol, which has an inhibitory effect on mycelial growth and spore germination, which can stagnate the growth cycle of microorganisms and cause apoptosis of some microbial cells; therefore, the two essential oils have different mechanisms of action on mold, and when combined together, they can produce a synergistic effect and produce a stronger antibacterial effect.

[0113] 4. Growth of three types of molds after 72 hours of combined use of cinnamon oil and peppermint oil

[0114] The culture time was extended to 72 hours, and the growth of mold on the plate was recorded. The results are as follows:

[0115] 4.1 Growth of Aspergillus niger after 72 hours of combined use of cinnamon oil and peppermint oil

[0116] Table 11 Growth of Aspergillus niger after 72h combined use of cinnamon oil and peppermint oil

[0117]

[0118] 4.2 Growth of Penicillium citrinum after 72 h of combined use of cinnamon oil and peppermint oil

[0119] Table 12 Growth of Penicillium citrinum after 72h combined use of cinnamon oil and peppermint oil

[0120]

[0121] 4.3 Growth of Trichoderma longipes after combined use of cinnamon oil and peppermint oil for 72 hours

[0122] Table 13 Growth of Trichoderma longipes after combined use of cinnamon oil and peppermint oil for 72 hours

[0123]

[0124] 5. Optimal essential oil ratio range (volume ratio)

[0125] Based on the 48h and 72h results of the combined antibacterial experiment, the FICI value ≤ 1 and the proportion of no growth at 72h were selected as the inhibition ratio of the three molds.

[0126] Table 14 Inhibition ratio of three kinds of mold

[0127] Peppermint oil: cinnamon oil volume ratio (v / v) Aspergillus niger 64:1～32:1 Penicillium citrinum 64:1～16:1 Trichoderma longipes 16:1

[0128] Taking all factors into consideration, when used in a conventional air environment, the inhibitory effect of the compound essential oil (essential oil composition) on the three molds may be in the range of peppermint oil: cinnamon oil (volume ratio) = 64:1 to 16:1; of course, essential oil compositions with other ratios may also be selected for specific environments.

[0129] 6. The peppermint oil and cinnamon oil used in the experiment were tested by GC-MS (gas chromatography-mass spectrometry) for their main components and contents (mass content).

[0130] 6.1 Cinnamon Oil

[0131] Table 15 Main components and contents of cinnamon oil

[0132]

[0133]

[0134] 6.2 Peppermint Oil

[0135] Table 16 Main components and contents of peppermint oil

[0136] Ingredients (Chinese name) English name Relative content (%) Menthone Menthone 23.5～37.5 Menthol Menthol 24.0～38.5 Menthyl acetate MenthylAcetate 0～6.00

[0137] Embodiment 2

[0138] This embodiment provides a specific use example of the plant essential oil composition.

[0139] Main instruments: airborne bacteria sampler (model: VWR SAS Super ISO 180); fumigator (model: SWX-M).

[0140] The main reagents of the experiment: Sabouraud nutrient agar medium (φ90 mm), purchased from Chongqing Pangtong Medical Instrument Co., Ltd.

[0141] Basic information of the fumigation room and selection of sampling points: The area of ​​the fumigation room is about 25m 2 , with a volume of approximately 80m 3 , refer to the sampling point selection method described in Appendix A of the national standard "GB / T 18204.3-2013 Public Place Hygiene Inspection Method Part 3: Air Microorganisms", and select a sampling point in the middle of the room for air microbial sampling.

[0142] Experimental steps:

[0143] Step 1: Mix peppermint oil and cinnamon oil in a volume ratio of 16:1 to make fumigation essential oil for later use.

[0144] Step 2: Before fumigation begins, close the door of the sampling space, place the airborne bacteria sampler at a height of 1.2m-1.5m from the ground, away from the vents, assemble Sabour nutrient agar medium (φ90mm), set the sampling volume to 500L, use the impact method to sample airborne bacteria, and sample each sampling point twice.

[0145] Step 3: After sampling before fumigation, 15 mL of the compounded essential oil is loaded into the agent compartment of the fumigator (model: SWX-M) to fumigate the room.

[0146] Step 4: Sample the air microorganisms in the room again according to step 2 at 2h and 5h after the start of fumigation.

[0147] Step 5: Place the three sampling culture media upside down in an incubator at 28°C and 70% relative humidity for 48 hours, and then count the number of molds grown on the sampling plates.

[0148] The fumigation sampling results are shown in Table 17 below:

[0149] Table 17 Fumigation sampling results

[0150] Fumigation time <![CDATA[Air mold count (CFU / M 3 )]]> Air mold purification rate (%) 0h 96 0 2h 57 40.63％ 5h 5 94.79％

[0151] It can be seen that by detecting the changes in the number of mold colonies before and after fumigation with compound plant essential oils, it can be found that the mold purification rate in the air reaches 40.63% in 2 hours and 94.79% in 5 hours, with good results.

[0152] Finally, it should be noted that this article uses specific examples to illustrate the principles and implementation methods of the present invention. The description of the above embodiments is only used to help understand the core idea of ​​the present invention. Without departing from the principle of the present invention, the present invention may also be improved and modified in a number of ways, and these improvements and modifications also fall within the scope of protection of the present invention; for example, in addition to being mixed to form fumigation essential oils, peppermint oil and cinnamon oil may also be mixed with existing solvents to form a sprayable, volatile solution.

Claims

1. A plant essential oil composition, characterized in that The preparation comprises the following raw materials: peppermint oil and cinnamon oil.

2. The plant essential oil composition according to claim 1, characterized in that: The volume ratio of the peppermint oil to the cinnamon oil is 64:1 to 16:

1.

3. The plant essential oil composition according to claim 1, characterized in that: The peppermint oil contains 23.5-37.5% of menthone, 24.0-38.5% of menthol and 0-6.00% of menthyl acetate.

4. The plant essential oil composition according to claim 1, characterized in that: The cinnamon oil contains 18.0-35.0% of limonene, 10.0-25.0% of cinnamaldehyde and 10.0-15.0% of linalool.

5. Use of the plant essential oil composition according to any one of claims 1 to 4 in the preparation of a medicament for inhibiting fungal activity.

6. The use according to claim 5, characterized in that: The molds include Aspergillus niger, Penicillium citrinum and / or Trichoderma longipedulum.

7. The use according to claim 5, characterized in that: The mold is Aspergillus niger, and the volume ratio of peppermint oil to cinnamon oil is 64:1-32:

1.

8. The use according to claim 5, characterized in that: The mold is Penicillium citrinum, and the volume ratio of peppermint oil to cinnamon oil is 64:1-16:

1.

9. The use according to claim 5, characterized in that: The mold is long-stalked Trichoderma, and the volume ratio of peppermint oil to cinnamon oil is 16:

1.

10. The use according to claim 5, characterized in that: The medicament is fumigation essential oil used in conjunction with a fumigator, and the fumigation essential oil is formed by mixing peppermint oil and cinnamon oil in a set volume ratio.

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