Composition with skin tendering, firming and anti-wrinkle effects and application thereof

Abstract

The invention relates to a composition with skin-tendering, firming and anti-wrinkle effects and application thereof. The composition with skin-tendering, firming and anti-wrinkle effects is prepared from bifidus yeast fermentation product filtrate, saccharomycetes / rice fermentation product filtrate and rose water. According to the composition disclosed by the invention, the bifidus yeast fermentation product filtrate, the saccharomycetes / rice fermentation product filtrate and the rose water are reasonably matched and have a synergistic effect, so that the composition has multiple effects of tendering skin, resisting wrinkles, tightening skin and the like.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and in particular relates to a composition with skin rejuvenation, firming and anti-wrinkle effects and an application thereof. Background Art

[0002] The skin consists of three layers: epidermis, dermis and subcutaneous tissue. The dermis contains a large amount of collagen and elastin, which keep the skin elastic and firm. As people age or are affected by the external environment, the production of protein in the skin will gradually decrease, which will cause the skin to lose elasticity and produce wrinkles. Wrinkles are the most direct manifestation of skin aging. Therefore, in order to slow down skin aging, more and more manufacturers are developing anti-wrinkle and skin-tightening cosmetics to reduce or prevent skin wrinkles.

[0003] CN118717555A discloses a composition with firming and anti-wrinkle effects, belonging to the field of cosmetic technology. The composition includes hydroxypropyl tetrahydropyrantriol, hydroxypinacolone retinoic acid ester, golden button extract, hydrolyzed hyaluronic acid, niacinamide, glutathione, carnosine, bisabolol, panthenol, bifid yeast fermentation product filtrate, aloe vera leaf water, and damask rose flower water. The composition rebuilds the skin dermis structure from different dimensions and the bottom layer of the skin from the inside out, supplements hyaluronic acid, and promotes the synthesis of hyaluronic acid and collagen, thereby achieving firming and anti-wrinkle skin care effects, and is stable, safe and effective.

[0004] CN114748374A discloses an anti-wrinkle composition and a preparation method thereof, and relates to the field of cosmetic technology. An anti-wrinkle composition, by weight, comprises the following raw materials: 0.01-5 parts of silk peptide, 0.001-2 parts of pentapeptide-3, 0.01-3 parts of palmitoyl pentapeptide-3, 0.001-0.01 parts of idebenone, 1-10 parts of lycopene and 10-20 parts of sea fennel extract. The preparation method is: mix idebenone and sea fennel extract, add glycerol, stir and heat, cool, then add lycopene to mix, and obtain a premixed solution; mix silk peptide, pentapeptide-3 and palmitoyl pentapeptide-3, then add the premixed solution, and stir and mix. The anti-wrinkle composition of the invention can improve skin wrinkles, promote fibroblast proliferation and collagen synthesis, inhibit tyrosinase activity and lipid peroxidation, and has significant anti-wrinkle, whitening and skin tightening effects. And its preparation method is simple, fast and easy to operate.

[0005] At present, although more active ingredients are added to the above-mentioned cosmetics, it is difficult to see obvious effects in a short period of time after the above-mentioned active ingredients are formulated. Therefore, it is necessary to develop a skin care product with more obvious and safer effects of anti-wrinkle, skin rejuvenation and skin firming. Summary of the invention

[0006] In view of the deficiencies of the prior art, the object of the present invention is to provide a composition having skin rejuvenation, firming and anti-wrinkle effects and its application.

[0007] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0008] In a first aspect, the present invention provides a composition with skin rejuvenation, firming and anti-wrinkle effects, wherein the composition with skin rejuvenation, firming and anti-wrinkle effects comprises: a filtrate of a bifida fermentation product, a filtrate of a yeast / rice fermentation product and rose water.

[0009] In the composition with skin rejuvenation, firming and anti-wrinkle effects of the present invention, the bifid yeast fermentation product filtrate contains small molecule amino acids, minerals, vitamin B group and the like, which can capture free radicals, strengthen the metabolism of the stratum corneum, shrink pores and make the skin more delicate; the yeast / rice fermentation product filtrate contains rich amino acids, proteins, polysaccharides, vitamins and antioxidant components, which can increase the moisture content of the skin, form a protective film to lock moisture, keep the skin soft, smooth and moisturized, and can also neutralize free radicals, reduce oxidative damage, promote the regeneration of the epidermis, and make the skin smoother and softer; the rose water has moisturizing properties, is suitable for use in the case of dry skin, and can play a moisturizing role; the rose water is also rich in antioxidant substances such as vitamin C, anthocyanins and polyphenols, which can effectively fight free radicals and thus have an excellent wrinkle removal effect; the three are reasonably matched and have a synergistic effect, so that the composition can have multiple functions such as skin rejuvenation, anti-wrinkle and skin firming.

[0010] Preferably, the mass ratio of the bifid yeast fermentation product filtrate, the yeast / rice fermentation product filtrate and rose water is (3-5):(1-4):(0.1-10).

[0011] Wherein "3-5" can be 3.1, 3.3, 3.5, 3.7, 3.9, 4, 4.1, 4.3, 4.5, 4.7 or 4.9, etc.;

[0012] “1-4” can be 1.2, 1.5, 1.8, 2, 2.2, 2.5, 2.8, 3, 3.2, 3.5 or 3.8, etc.;

[0013] “0.1-10” can be 0.2, 0.4, 0.6, 0.8, 1, 1.52, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9 or 9.5, etc.

[0014] Preferably, the composition with skin rejuvenation, firming and anti-wrinkle effects further comprises any one of hydroxypropyl tetrahydropyrantriol, tetrahydromethylpyrimidine carboxylic acid or Nannochloropsis oculi extract or a combination of at least two thereof.

[0015] Preferably, the composition with skin rejuvenation, firming and anti-wrinkle effects also includes hydroxypropyl tetrahydropyrantriol, tetrahydromethylpyrimidine carboxylic acid and Nannochloropsis oculiformis extract.

[0016] The hydroxypropyl tetrahydropyrantriol in the composition of the present invention contains a large number of hydroxyl (-OH) groups, which can absorb surrounding moisture and retain the moisture on the skin surface, thereby preventing moisture loss, and it also has a strong antioxidant effect; the tetrahydromethylpyrimidine carboxylic acid and the eye-shaped vein microalgae extract both have excellent moisturizing effects, can stabilize the skin cell structure, help the skin lock in moisture, maintain a moist state, and can also resist oxidation and improve skin elasticity; the three cooperate synergistically to further improve the skin rejuvenation, anti-wrinkle and skin tightening effects of the composition.

[0017] Preferably, the composition with skin rejuvenation, firming and anti-wrinkle effects comprises, by weight, 15-25 parts of bifida fermentation product filtrate, 5-20 parts of yeast / rice fermentation product filtrate, 5-10 parts of rose water, 1-2 parts of hydroxypropyl tetrahydropyrantriol, 1-5 parts of tetrahydromethylpyrimidine carboxylic acid and 0.1-2 parts of Nannochloropsis oculata extract.

[0018] The amount of the filtrate of the bifid yeast fermentation product in the composition with skin rejuvenation, firming and anti-wrinkle effects of the present invention can be 16 parts, 17 parts, 18 parts, 19 parts, 20 parts, 21 parts, 22 parts, 23 parts or 24 parts, etc.;

[0019] The amount of the yeast / rice fermentation product filtrate added can be 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, 15 parts, 16 parts, 17 parts, 18 parts or 19 parts, etc.;

[0020] The amount of the rose water added can be 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts, 8.5 parts, 9 parts or 9.5 parts, etc.;

[0021] The addition amount of the hydroxypropyl tetrahydropyrantriol can be 1.1 parts, 1.2 parts, 1.3 parts, 1.4 parts, 1.5 parts, 1.6 parts, 1.7 parts, 1.8 parts or 1.9 parts, etc.;

[0022] The amount of the ectoine carboxylic acid added can be 1.2 parts, 1.5 parts, 1.8 parts, 2 parts, 2.2 parts, 2.5 parts, 2.8 parts, 3 parts, 3.2 parts, 3.5 parts, 3.8 parts, 4 parts, 4.2 parts, 4.5 parts or 4.8 parts, etc.;

[0023] The added amount of the Nannochloropsis oculiformis extract can be 0.2 parts, 0.4 parts, 0.6 parts, 0.8 parts, 1 parts, 1.2 parts, 1.4 parts, 1.6 parts or 1.8 parts, etc.

[0024] Other specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0025] In a second aspect, the present invention provides a use of the composition with skin rejuvenation, firming and anti-wrinkle effects as described in the first aspect in the preparation of cosmetics.

[0026] Preferably, the cosmetics include any one of essence, essence milk, face cream, face cleanser or lotion.

[0027] Preferably, the mass percentage of the composition with skin rejuvenation, firming and anti-wrinkle effects in the cosmetic is 1-15%, for example, it can be 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13% or 14%, etc.

[0028] Other specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0029] In a third aspect, the present invention provides an essence, the components of which include the composition with skin rejuvenation, firming and anti-wrinkle effects as described in the first aspect, a moisturizer, an emulsifier, a thickener and water.

[0030] Preferably, the essence comprises 1-15 parts of the composition with skin rejuvenation, firming and anti-wrinkle effects as described in the first aspect, 10-30 parts of moisturizer, 1-10 parts of emulsifier, 0.1-2 parts of thickener and 45-90 parts of water.

[0031] The amount of the composition with skin rejuvenation, firming and anti-wrinkle effects in the essence of the present invention can be 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts or 14 parts, etc.;

[0032] The amount of the moisturizer added can be 11 parts, 13 parts, 15 parts, 17 parts, 19 parts, 20 parts, 23 parts, 25 parts, 27 parts or 29 parts, etc.;

[0033] The amount of the emulsifier added can be 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts or 9 parts, etc.;

[0034] The thickener may be added in an amount of 0.3 parts, 0.5 parts, 0.7 parts, 0.9 parts, 1 parts, 1.2 parts, 1.5 parts, 1.7 parts or 1.9 parts, etc.;

[0035] The amount of water added can be 50 parts, 55 parts, 60 parts, 65 parts, 70 parts, 75 parts, 80 parts or 85 parts, etc.

[0036] Preferably, the moisturizing agent comprises any one of mucopolysaccharide, polypeptide or complex ceramide, or a combination of at least two thereof.

[0037] Preferably, the moisturizing agent comprises mucopolysaccharide, polypeptide and complex ceramide.

[0038] In the present invention, the mucopolysaccharide, polypeptide and composite ceramide are used in combination, which can not only provide more nutrition and energy for skin cells, but also deeply moisturize the skin. After being added to the essence, they can cooperate with the composition with skin rejuvenation, firming and anti-wrinkle effects to further improve the moisturizing and skin rejuvenation effect of the essence.

[0039] Preferably, the mass ratio of the mucopolysaccharide, polypeptide and complex ceramide is (0.5-5):(0.5-10):(1-5).

[0040] Wherein “0.5-5” can be 0.8, 1, 1.2, 1.5, 1.7, 2, 2.2, 2.5, 2.8, 3, 3.2, 3.5, 3.8, 4, 4.2, 4.5 or 4.8, etc.;

[0041] “0.5-10” can be 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9 or 9.5, etc.;

[0042] “1-5” can mean 1.2, 1.5, 1.7, 2, 2.2, 2.5, 2.8, 3, 3.2, 3.5, 3.8, 4, 4.2, 4.5 or 4.8, etc.

[0043] Preferably, the complex ceramide comprises ceramide 2, ceramide 3, ceramide 6 and ceramide 7.

[0044] Preferably, the emulsifier comprises polyglycerol esters and / or PEG / PPG-14 / 7 dimethyl ether.

[0045] Preferably, the emulsifier comprises polyglycerol esters and PEG / PPG-14 / 7 dimethyl ether.

[0046] Preferably, the mass ratio of the polyglycerol esters to PEG / PPG-14 / 7 dimethyl ether is (1-2):(0.1-1).

[0047] Wherein "1-2" can be 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8 or 1.9, etc.;

[0048] “0.1-1” can be 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8 or 0.9, etc.

[0049] Preferably, the polyglycerol esters include polyglycerol fatty acid esters and / or polyglycerol ricinoleate.

[0050] Preferably, the thickener includes any one of xanthan gum, guar gum, hydroxyethyl cellulose or sodium acrylate / sodium acryloyldimethyl taurate copolymer or a combination of at least two thereof.

[0051] Other specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0052] In a fourth aspect, the present invention provides a method for preparing the essence according to the third aspect, the preparation method comprising:

[0053] (1) stirring water and a moisturizer, heating them up, and then keeping them warm to obtain an aqueous phase liquid; stirring an emulsifier and a thickener, heating them up, and then keeping them warm to obtain an oil phase liquid;

[0054] (2) After the heat preservation is completed, the aqueous phase liquid and the oil phase liquid are mixed and homogenized, the temperature is lowered, and the composition with skin rejuvenation, firming and anti-wrinkle effects is added, and the essence is obtained after homogenization.

[0055] Preferably, during the preparation of the aqueous phase liquid in step (1), the temperature is raised to 80-85°C (for example, 81°C, 82°C, 83°C, 84°C or 85°C, etc.) and kept warm for 1-3h (for example, 1.1h, 1.5h, 1.8h, 2h, 2.2h, 2.5h or 2.8h, etc.).

[0056] Preferably, during the preparation of the oil phase liquid in step (1), the temperature is raised to 75-85°C (for example, 76°C, 78°C, 80°C, 82°C or 84°C, etc.) and kept warm for 1-3h (for example, 1.1h, 1.5h, 1.8h, 2h, 2.2h, 2.5h or 2.8h, etc.).

[0057] Preferably, the homogenization temperature in step (2) is 75-85°C, for example, 76°C, 78°C, 80°C, 82°C or 84°C.

[0058] Preferably, the cooling in step (2) refers to cooling to 30-40°C, for example, 31°C, 33°C, 35°C, 37°C or 39°C.

[0059] Other specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0060] Compared with the prior art, the present invention has the following beneficial effects:

[0061] (1) The composition with skin rejuvenation, firming and anti-wrinkle effects of the present invention comprises the bifid yeast fermentation product filtrate, the yeast / rice fermentation product filtrate and rose water, which are reasonably matched to have a synergistic effect, so that the composition can have multiple effects such as skin rejuvenation, anti-wrinkle and skin firming.

[0062] (2) The essence of the present invention can provide more nutrition and energy to skin cells and deeply moisturize the skin by combining the composition with skin rejuvenation, firming and anti-wrinkle effects with components such as moisturizers and emulsifiers, thereby having excellent anti-wrinkle and skin firming effects.

[0063] (3) The preparation method of the essence provided by the present invention has simple steps, is more energy-saving and environmentally friendly, and is suitable for large-scale production. DETAILED DESCRIPTION

[0064] In order to further explain the technical means and effects adopted by the present invention, the technical solution of the present invention is further described below in conjunction with the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.

[0065] The following materials and comparative examples are as follows:

[0066]

[0067] The rest of the raw materials can be used as long as they are purchased from regular dealers.

[0068] Example 1

[0069] The present embodiment provides a composition with skin rejuvenation, firming and anti-wrinkle effects. The components of the composition with skin rejuvenation, firming and anti-wrinkle effects are, by weight, 20 parts of bifida fermentation product filtrate, 15 parts of yeast / rice fermentation product filtrate, 8 parts of rose water, 1.5 parts of hydroxypropyl tetrahydropyrantriol, 3 parts of tetrahydromethylpyrimidine carboxylic acid and 1.5 parts of Nannochloropsis oculata extract.

[0070] Example 2

[0071] The present embodiment provides a composition with skin rejuvenation, firming and anti-wrinkle effects. The components of the composition with skin rejuvenation, firming and anti-wrinkle effects are, by weight, 25 parts of bifida fermentation product filtrate, 10 parts of yeast / rice fermentation product filtrate, 10 parts of rose water, 1 part of hydroxypropyl tetrahydropyrantriol, 5 parts of tetrahydromethylpyrimidine carboxylic acid and 1 part of Nannochloropsis oculata extract.

[0072] Example 3

[0073] The present embodiment provides a composition with skin rejuvenation, firming and anti-wrinkle effects. The components of the composition with skin rejuvenation, firming and anti-wrinkle effects are, by weight, 15 parts of bifida fermentation product filtrate, 20 parts of yeast / rice fermentation product filtrate, 5 parts of rose water, 2 parts of hydroxypropyl tetrahydropyrantriol, 2 parts of tetrahydromethylpyrimidine carboxylic acid and 2 parts of Nannochloropsis oculi extract.

[0074] Example 4

[0075] This embodiment provides a composition with skin rejuvenation, firming and anti-wrinkle effects. The components of the composition with skin rejuvenation, firming and anti-wrinkle effects are 20 parts of bifida fermentation product filtrate, 15 parts of yeast / rice fermentation product filtrate and 8 parts of rose water in parts by weight.

[0076] Example 5

[0077] The present embodiment provides a composition with skin rejuvenation, firming and anti-wrinkle effects. The components of the composition with skin rejuvenation, firming and anti-wrinkle effects are 20 parts of bifida fermentation product filtrate, 15 parts of yeast / rice fermentation product filtrate, 8 parts of rose water, 2 parts of hydroxypropyl tetrahydropyrantriol and 4 parts of tetrahydromethylpyrimidine carboxylic acid in parts by weight.

[0078] Example 6

[0079] The present embodiment provides a composition with skin rejuvenation, firming and anti-wrinkle effects. The components of the composition with skin rejuvenation, firming and anti-wrinkle effects are, by weight, 20 parts of bifida fermentation product filtrate, 15 parts of yeast / rice fermentation product filtrate, 8 parts of rose water, 3 parts of hydroxypropyl tetrahydropyrantriol and 3 parts of Nannochloropsis oculiformis extract.

[0080] Example 7

[0081] The present embodiment provides a composition with skin rejuvenation, firming and anti-wrinkle effects. The components of the composition with skin rejuvenation, firming and anti-wrinkle effects are, by weight, 20 parts of bifida fermentation product filtrate, 15 parts of yeast / rice fermentation product filtrate, 8 parts of rose water, 4 parts of tetrahydromethylpyrimidine carboxylic acid and 2 parts of Nannochloropsis oculi extract.

[0082] Comparative Example 1

[0083] This comparative example provides a composition with skin rejuvenation, firming and anti-wrinkle effects. The components of the composition with skin rejuvenation, firming and anti-wrinkle effects do not include the filtrate of the bifid yeast fermentation product, and the reduced amount thereof is distributed to the yeast / rice fermentation product filtrate and rose water in proportion, and the other components and amounts remain unchanged.

[0084] Comparative Example 2

[0085] This comparative example provides a composition with skin rejuvenation, firming and anti-wrinkle effects. The components of the composition with skin rejuvenation, firming and anti-wrinkle effects do not include yeast / rice fermentation product filtrate, and the reduced amount thereof is distributed to bifida fermentation product filtrate and rose water in proportion, while the other components and amounts remain unchanged.

[0086] Comparative Example 3

[0087] This comparative example provides a composition with skin rejuvenation, firming and anti-wrinkle effects, wherein the composition with skin rejuvenation, firming and anti-wrinkle effects does not include rose water, and the reduced amount thereof is distributed to the yeast / rice fermentation product filtrate and the bifid yeast fermentation product filtrate in proportion, while the other components and amounts remain unchanged.

[0088] Application Examples 1-3

[0089] This application example provides three kinds of essences, and the formulas thereof are as follows. The dosage units of each component in the table are all “portions”.

[0090]

[0091]

[0092] The composite ceramide comprises ceramide 2, ceramide 3, ceramide 6 and ceramide 7 in a mass ratio of 1:1:3:1.

[0093] Its preparation method is:

[0094] (1) stirring water and a moisturizing agent, heating the mixture to 80° C., and then maintaining the temperature for 2 h to obtain an aqueous phase liquid;

[0095] (2) stirring the emulsifier and thickener and heating them to 80° C. for 2 h to obtain an oil phase liquid;

[0096] (2) After the heat preservation is completed, the aqueous phase liquid and the oil phase liquid are mixed and homogenized at 80° C., and after cooling to 35° C., the composition with skin rejuvenation, firming and anti-wrinkle effects is added, and the essence is obtained after homogenization.

[0097] Application Example 4-7

[0098] This application example provides four kinds of essences. The difference between the essences and application example 1 is that the composition with skin rejuvenation, firming and anti-wrinkle effects of example 1 is replaced by the composition with skin rejuvenation, firming and anti-wrinkle effects of examples 4-7 in equal amounts. The other components and amounts are consistent with those in application example 1. The preparation method refers to application example 1.

[0099] Application Example 8

[0100] This application example provides an essence, the essence differing from application example 1 only in that the moisturizer does not include complex ceramide, and the reduced amount thereof is distributed to mucopolysaccharides and polypeptides in proportion, and the remaining components and amounts are consistent with those in application example 1, and the preparation method refers to application example 1.

[0101] Application Example 9

[0102] This application example provides an essence, the essence differing from application example 1 only in that the moisturizer does not include polypeptides, and the reduced amount thereof is distributed to the complex ceramide and mucopolysaccharide in proportion, and the remaining components and amounts are consistent with those in application example 1, and the preparation method refers to application example 1.

[0103] Application Example 10

[0104] This application example provides an essence, the essence differing from application example 1 only in that mucopolysaccharide is not included in the moisturizing agent, and the reduced amount is distributed to the composite ceramide and the polypeptide in proportion, and the remaining components and amounts are consistent with those in application example 1, and the preparation method refers to application example 1.

[0105] Comparative Application Examples 1-3

[0106] This application example provides three kinds of essences. The difference between the essences and application example 1 is that the composition with skin rejuvenation, firming and anti-wrinkle effects of example 1 is replaced by the compositions with skin rejuvenation, firming and anti-wrinkle effects of comparative examples 1-3 in equal amounts, and the other components and amounts are consistent with those of application example 1. The preparation method refers to application example 1.

[0107] Comparative Application Example 4

[0108] This application example provides an essence, which differs from Application Example 1 only in that the essence does not include the composition with skin rejuvenation, firming and anti-wrinkle effects of Example 1, and the reduced amount is supplemented by water. The remaining components and amounts are consistent with those of Application Example 1, and the preparation method refers to Application Example 1.

[0109] Test Example 1 Antioxidant Efficacy Study - DPPH Free Radical Scavenging Experiment

[0110] Test samples: samples provided in Application Examples 1-10 and Comparative Application Examples 1-4;

[0111] Test method:

[0112] Accurately weigh 47.7 mg of DPPH reagent, dilute to a 100 ml brown volumetric flask, take 5 ml of it and dilute to a 50 ml brown volumetric flask with anhydrous ethanol to obtain DPPH test solution (47.7 mg / L). Pipette 2 mL of DPPH test solution respectively, add 0.1 mL of the sample provided in Application Examples 1-10 and Comparative Application Examples 1-4 as shown in the table below, and then add an appropriate amount of anhydrous ethanol to make the total mass equal. After fully mixing, let it stand in the dark for 30 minutes, use anhydrous ethanol as a blank control, measure its absorbance at 517 nm, and set vitamin C as a control, and calculate the scavenging rate of each extract for DPPH according to the following formula:

[0113] DPPH removal rate (%) = [1-(As-Ar) / A0] × 100%

[0114] Where: As is the absorbance of the extract and DPPH reaction system; Ar is the absorbance of the extract-anhydrous ethanol; A0 is the absorbance of the blank control anhydrous ethanol.

[0115]

[0116] The DPPH free radical scavenging rate reflects its antioxidant capacity to a certain extent. The higher the scavenging rate, the better the antioxidant degree and the better the anti-aging effect. Therefore, the anti-aging effect of the essence can be judged by studying its ability to scavenge DPPH free radicals. Vitamin C is a highly effective antioxidant recognized by the cosmetics industry. This experiment used vitamin C as a control to examine its antioxidant capacity. The test results are shown in Table 1.

[0117] Table 1

[0118]

[0119]

[0120] As can be seen from Table 1, compared with Comparative Application Example 4, the essences of Application Examples 1-3 have better scavenging effects on DPPH, and the scavenging rates are all above 88%, while the scavenging effects of Application Examples 4-7 on DPPH are reduced, indicating that the addition of hydroxypropyl tetrahydropyrantriol, tetrahydromethylpyrimidine carboxylic acid and eye-shaped veined Nannochloropsis algae extract to the composition can make the antioxidant effect of the essence better, and the simultaneous presence of hydroxypropyl tetrahydropyrantriol, tetrahydromethylpyrimidine carboxylic acid and eye-shaped veined Nannochloropsis algae extract can better improve the anti-wrinkle and firming effect; the scavenging effects on DPPH in Comparative Application Examples 1-3 are reduced, indicating that the three components of the bifida fermentation product filtrate, the yeast / rice fermentation product filtrate and the rose water promote each other in terms of antioxidant properties and have a synergistic effect.

[0121] Test Example 2: Wrinkle removal and skin firming test

[0122] The skin tightening effect of the essence prepared by using Example 1-10 and Comparative Application Example 1-4 was evaluated:

[0123] 140 trial subjects aged 20-45 were randomly selected and divided into 14 groups, with 10 in each group. The 15 groups of trial subjects used the essence prepared in Application Examples 1-10 and Comparative Application Examples 1-4 (once in the morning and once in the evening) for 30 days. During the trial, the subjects were not allowed to use other items to maintain skin elasticity and cosmetics that affect the results, and did not change their original eating habits during the trial.

[0124] Cutometer MPA580 was used to test the skin condition on the 0th and 30th days. The test site was the zygomatic region. The probe was measured three times in the same test area and the average value was taken. The test parameter is R2 (the ratio of the skin rebound amount Ua without negative pressure to the maximum stretch amount Uf with negative pressure). The closer R2 is to 1, the better the skin elasticity. The average change value of R2 = R2 value on the 30th day - R2 value on the 0th day, and the average change rate of R2 = R2 average change value / R2 value on the 0th day. The specific skin elasticity test results are shown in Table 2.

[0125] Table 2

[0126]

[0127]

[0128] As can be seen from Table 2, the essence of the present invention has a good skin tightening effect, wherein the three components of the bifid yeast fermentation product filtrate, the yeast / rice fermentation product filtrate and the rose water promote each other in this effect, and have an unexpected synergistic effect, and the addition of hydroxypropyl tetrahydropyrantriol, tetrahydromethylpyrimidine carboxylic acid and the eye-shaped vein Nannochloropsis oculata extract can make the skin tightening effect of the composition better.

[0129] Test Example 3: VISIA Skin Analysis and Evaluation Experiment

[0130] The VISIA skin image analyzer uses multi-spectral imaging technology. Through a 12-megapixel camera, it can shoot from three angles: the front, left side, and right side. It can be imaged three times under three light sources: standard white light, cross-sectional polarized light, and ultraviolet light. Therefore, the VISIA facial image analysis system can systematically analyze the pigmentation spots, textures, pores, wrinkles, ultraviolet light spots, stable pigment spots, red areas, and rhodopsin on the subject's facial skin, providing an accurate and quantitative analysis basis for the skin. By recording the pigmentation spots, wrinkles, and textures on the face of the subject before and after the test, the absolute score represents the area and intensity of the skin characteristic detection value, which is used to observe the changes in skin properties. Therefore, the data obtained by the absolute score (the average of the test results of the front, left side, and right side) is selected as the analysis standard and data statistical basis. All the data of each subject are entered into the computer, the test results are compared, and the skin characteristics and various index data of the subject are analyzed through statistical processing. The test instrument used in the experiment is the VISIA facial image analysis system (CANFIELD Technology Company, USA).

[0131] The volunteers and test example 2 are the same group of volunteers. They use the essences prepared in application examples 1-10 and comparative application examples 1-4 respectively. The subjects need to use them continuously, apply the products twice a day, morning and evening, for 28 consecutive days, and test them on day 0 (D0), day 14 (D14) and day 28 (D28). During the test, the subjects are not allowed to use other items to maintain skin elasticity and cosmetics that affect the results, and do not change their original eating habits during the test. The test of the same subject is completed by the same measurement personnel.

[0132] The specific test method is:

[0133] After the subjects thoroughly cleaned their faces with a uniform facial cleanser, they gently wiped their faces dry with a paper towel, and waited for 15 minutes in a room with a room temperature of 22°C ± 2°C and a relative humidity of 40 ± 2%. Then, the VISIA facial image analyzer was used to take photos of the test subjects' faces. The analysis area should try to avoid interference from reflective areas and hair. The subjects' faces (including the front, left side, and right side) were photographed, and the absolute scores obtained were statistically analyzed to record the test data of the texture and wrinkles on the subjects' faces. The test results are shown in Table 3.

[0134] Table 3

[0135]

[0136]

[0137] As shown in Table 3, compared with Comparative Application Example 4, the essences of Application Examples 1-3 have obvious improvement effects on the wrinkles and textures of the subjects, indicating that the essence provided by the present invention can achieve good wrinkle removal and texture smoothing effects by adding a composition with skin rejuvenation, firming and anti-wrinkle effects, while the improvement effects of Application Examples 4-7 are reduced, indicating that the addition of hydroxypropyl tetrahydropyrantriol, tetrahydromethylpyrimidine carboxylic acid and eye-shaped vein Nannochloropsis algae extract in the composition can make the wrinkle removal and firming effects of the essence better, and the simultaneous presence of hydroxypropyl tetrahydropyrantriol, tetrahydromethylpyrimidine carboxylic acid and eye-shaped vein Nannochloropsis algae extract can better improve the anti-wrinkle and firming effects; in Application Examples 8-10, the choice of moisturizer also affects the anti-wrinkle and firming effects of the essence; the improvement effect in Comparative Application Examples 1-3 is reduced, indicating that the three components of the bifida fermentation product filtrate, the yeast / rice fermentation product filtrate and the rose water promote each other in improving wrinkles and textures, and have a synergistic effect.

[0138] Test Example 4: Safety Evaluation Experiment

[0139] The specific method is as follows: 40 females aged 18-35 years old with no history of allergies were selected, and each subject was applied with the samples prepared in Examples 1-10. After cleaning the back of the subject, the spot tester with the sample was applied to the selected position on the back with a non-irritating tape, and after pasting, it was lightly pressed with fingers to make it evenly attached to the skin for 48 hours. The subject kept the patch area dry within 48 hours, and avoided intense exercise, scratching the patch area, long-term sunlight exposure, etc. After 48 hours, the tester was removed and marked, and after 30 minutes, the indentation disappeared and the judgment was made under sufficient light.

[0140] The grading standards for adverse skin reactions are shown in Table 4:

[0141] Table 4

[0142]

[0143] The results are shown in Table 5:

[0144] Table 5

[0145]

[0146]

[0147] It can be seen from the data in Table 5 that after removing the spot tester, no adverse reactions were observed on the skin of the volunteers, which indicates that the raw materials used in the essence of the present invention are safe and non-irritating, have no adverse reactions to the human body, and are highly safe.

[0148] The applicant declares that the present invention uses the above-mentioned embodiments to illustrate the composition with skin rejuvenation, firming and anti-wrinkle effects and its application, but the present invention is not limited to the above-mentioned embodiments, that is, it does not mean that the present invention must rely on the above-mentioned embodiments to be implemented. Those skilled in the art should understand that any improvement of the present invention, equivalent replacement of various raw materials of the product of the present invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

[0149] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, a variety of simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0150] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. A composition having skin rejuvenation, firming and anti-wrinkle effects, characterized in that: The composition with skin rejuvenation, firming and anti-wrinkle effects comprises: bifida yeast fermentation product filtrate, yeast / rice fermentation product filtrate and rose water.

2. The composition with skin rejuvenation, firming and anti-wrinkle effects as claimed in claim 1, characterized in that: The mass ratio of the bifid yeast fermentation product filtrate, the yeast / rice fermentation product filtrate and the rose water is (3-5):(1-4):(0.1-10).

3. The composition with skin rejuvenation, firming and anti-wrinkle effects as claimed in claim 1 or 2, characterized in that: The composition with skin rejuvenation, firming and anti-wrinkle effects also includes any one of hydroxypropyl tetrahydropyrantriol, tetrahydromethylpyrimidine carboxylic acid or Nannochloropsis oculi extract or a combination of at least two thereof; Preferably, the composition with skin rejuvenation, firming and anti-wrinkle effects also includes hydroxypropyl tetrahydropyrantriol, tetrahydromethylpyrimidine carboxylic acid and Nannochloropsis oculiformis extract.

4. The composition with skin rejuvenation, firming and anti-wrinkle effects as claimed in claim 3, characterized in that: The composition with skin rejuvenation, firming and anti-wrinkle effects comprises, by weight, 15-25 parts of bifida fermentation product filtrate, 5-20 parts of yeast / rice fermentation product filtrate, 5-10 parts of rose water, 1-2 parts of hydroxypropyl tetrahydropyrantriol, 1-5 parts of tetrahydromethylpyrimidine carboxylic acid and 0.1-2 parts of Nannochloropsis oculata extract.

5. Use of the composition with skin rejuvenation, firming and anti-wrinkle effects as claimed in any one of claims 1 to 4 in the preparation of cosmetics.

6. The use according to claim 5, characterized in that The cosmetics include any one of essence, essence milk, face cream, face cleanser or lotion; Preferably, the composition having skin rejuvenation, firming and anti-wrinkle effects has a mass percentage of 1-15% in the cosmetic.

7. An essence, characterized in that: The components of the essence include the composition with skin rejuvenation, firming and anti-wrinkle effects as described in any one of claims 1 to 4, a moisturizer, an emulsifier, a thickener and water.

8. The essence according to claim 7, characterized in that The essence comprises 1-15 parts of the composition with skin rejuvenation, firming and anti-wrinkle effects as claimed in any one of claims 1-4, 10-30 parts of moisturizer, 1-10 parts of emulsifier, 0.1-2 parts of thickener and 45-90 parts of water; Preferably, the moisturizing agent comprises any one or a combination of at least two of mucopolysaccharides, polypeptides or complex ceramides; Preferably, the moisturizing agent includes mucopolysaccharide, polypeptide and complex ceramide; Preferably, the composite ceramide comprises ceramide 2, ceramide 3, ceramide 6 and ceramide 7; Preferably, the emulsifier comprises polyglycerol esters and / or PEG / PPG-14 / 7 dimethyl ether; Preferably, the emulsifier includes polyglycerol esters and PEG / PPG-14 / 7 dimethyl ether; Preferably, the polyglycerol esters include polyglycerol fatty acid esters and / or polyglycerol ricinoleate; Preferably, the thickener includes any one of xanthan gum, guar gum, hydroxyethyl cellulose or sodium acrylate / sodium acryloyldimethyl taurate copolymer or a combination of at least two thereof.

9. A method for preparing the essence according to claim 7 or 8, characterized in that: The preparation method comprises: (1) stirring water and a moisturizer, heating them up, and then keeping them warm to obtain a water phase liquid; stirring an emulsifier and a thickener, heating them up, and then keeping them warm to obtain an oil phase liquid; (2) After the heat preservation is completed, the aqueous phase liquid and the oil phase liquid are mixed and homogenized, the temperature is lowered, and the composition with skin rejuvenation, firming and anti-wrinkle effects is added, and the essence is obtained after homogenization.

10. The preparation method according to claim 9, characterized in that: When preparing the aqueous phase liquid in step (1), the temperature is raised to 80-85° C. and kept warm for 1-3 hours; Preferably, the oil phase liquid in step (1) is heated to 75-85° C. and kept warm for 1-3 hours; Preferably, the homogenization temperature in step (2) is 75-85°C; Preferably, the cooling in step (2) refers to cooling to 30-40°C.

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