Preparation method of biological cell polypeptide

Abstract

The invention belongs to the technical field of bioengineering, and particularly relates to a biological cell polypeptide preparation method which comprises the following steps: S1, preparing a laboratory; s2, preparing biological cell polypeptide; s3, selecting two of whey powder, fish meal and fresh milk as raw materials for preparing biological cell polypeptide; s4, crushing and mixing the selected two combined preparation raw materials, and then carrying out mixed material conveying; and S5, selecting a combination of trypsin, pepsin and chymosin as a biological enzyme for preparing the biological cell polypeptide, and carrying out enzyme dissolution and transportation. Two of the whey powder, the fish meal and the fresh milk are selected as preparation raw materials of the biological cell polypeptide, a plurality of samples are provided, and after the biological cell polypeptide is prepared, an experimenter can conveniently compare and record the biological cell polypeptide; therefore, experimenters can preferentially select two of the whey powder, the fish meal and the fresh milk to prepare the raw materials.

Description

Technical Field

[0001] The present invention relates to the field of bioengineering technology, and in particular to a method for preparing a biological cell polypeptide. Background Art

[0002] Peptides are compounds formed by α-amino acids linked together by peptide bonds. They are intermediate products of protein hydrolysis. Generally, the number of amino acids contained in peptides is from two to nine. Depending on the number of amino acids in the peptide, peptides have different names: dipeptides, tripeptides, tetrapeptides, pentapeptides, etc. Peptides composed of three or more amino acid molecules are called polypeptides. Their molecular weight is less than 10,000Da, they can pass through semipermeable membranes, and are not precipitated by trichloroacetic acid and ammonium sulfate.

[0003] With the continuous improvement of people's living standards, the demand for cosmetics market is also constantly improving, and functional cosmetics are also highly respected. Bioactive peptide compounds have the advantages of high biological activity, easy absorption, safety and non-toxicity. Their physiological and biological effects of beauty, skin care and anti-aging are also gradually applied to beauty cosmetics. At present, the bioactive peptides widely used in beauty, skin care and cosmetics are mainly functional peptides with small molecular weight, and most of these peptides are prepared by chemical synthesis. However, in the actual preparation process, the selected raw materials are relatively limited, and the prepared biological cell peptide samples are usually relatively small, which is not conducive to the experimenters to compare and then select the best. Summary of the invention

[0004] In view of the fact that the raw materials selected for preparation in the actual preparation process are relatively limited, the biological cell polypeptide samples prepared are usually relatively small, which is not conducive to the experimenters to compare and then select the best technical problem, the present invention proposes a method for preparing biological cell polypeptides.

[0005] The present invention provides a method for preparing a biological cell polypeptide, comprising the following steps:

[0006] S1: Prepare the laboratory;

[0007] S2: Preparation of biological cell polypeptides;

[0008] S3: selecting two of whey powder, fish meal, and fresh milk as raw materials for preparing biological cell polypeptides;

[0009] S4: crushing and mixing the selected two combinations of the prepared raw materials, and then conveying the mixed materials;

[0010] S5: selecting a combination of trypsin, pepsin and chymosin as biological enzymes for preparing biological cell polypeptides, and performing enzyme dissolution and delivery;

[0011] S6: adding the two selected raw materials and the biological enzyme into a constant temperature oscillator for enzymolysis. After the enzymolysis is completed, the temperature is raised to 95° C., the enzyme is inactivated for 10 minutes, and the mixture is cooled to room temperature.

[0012] S7: adding the raw material into a tubular centrifuge device for centrifugation, and after centrifugation, performing oil phase, liquid phase and solid phase separation, and then filtering and decolorizing;

[0013] S8: Ultrafiltration is performed through an ultrafiltration membrane, and the obtained ultrafiltrate is freeze-dried to obtain a biological polypeptide dry powder, which is quantitatively packaged and stored at a low temperature for later use.

[0014] As a preferred embodiment of the present invention, in S1: the laboratory can be selected from a chemical laboratory or a biological laboratory, and the grade of the laboratory is at least a P2 laboratory, and the laboratory environment requires a constant range of temperature, humidity and pressure difference.

[0015] As a preferred embodiment of the present invention, in S3: two of the selected preparation raw materials among whey powder, fish meal, and fresh milk can be: whey powder and fish meal, whey powder and fresh milk, fish meal and fresh milk.

[0016] As a preferred embodiment of the present invention, in S4: the selected raw materials of the combination of whey powder and fish meal, whey powder and fresh milk, or fish meal and fresh milk are crushed and mixed, and then the mixed materials are transported.

[0017] As a preferred embodiment of the present invention, in S6: the constant temperature oscillator is kept at 50°C, and the enzymatic hydrolysis is carried out in the constant temperature oscillator at 50°C for 6 hours.

[0018] As a preferred embodiment of the present invention, in S6: the constant temperature oscillator is kept at 50°C, and the enzymatic hydrolysis is carried out in the constant temperature oscillator at 50°C for 7 hours.

[0019] As a preferred embodiment of the present invention, in S6: the constant temperature oscillator is kept at 50°C, and the enzymatic hydrolysis is carried out in the constant temperature oscillator at 50°C for 8 hours.

[0020] As a preferred embodiment of the present invention, in S7: the centrifugation temperature of the tubular centrifuge is 2-6°C.

[0021] The beneficial effects of the present invention are:

[0022] The present invention selects two of whey powder, fish meal and fresh milk as raw materials for preparing biological cell polypeptides, and has multiple samples. After the preparation of the biological cell polypeptides is completed, it is convenient for experimenters to compare and record, so that the experimenters can preferentially select two of the preparation raw materials among whey powder, fish meal and fresh milk, and the selected biological enzymes of trypsin, pepsin and chymosin combination are enzymolyzed in a 50°C constant temperature oscillator for 6h, 7h and 8h, so that the experimenters can observe the enzymolysis effect of the biological enzymes of trypsin, pepsin and chymosin combination in a 50°C constant temperature oscillator for 6h, 7h and 8h, thereby This makes it easy for experimenters to compare the enzymatic hydrolysis effects at different times, and thus allows experimenters to preferentially select the enzymatic hydrolysis time of the combination of trypsin, pepsin and rennet in a 50°C constant temperature oscillator, so that the prepared biological cell polypeptide has the advantages of safety, stability, water solubility, easy absorption, and small molecular weight. It also has multiple biological activities such as free radical scavenging, anti-oxidation, and anti-aging. It can not only promote the proliferation of skin cells, but also provide nutrition for the skin, delay skin aging, and promote skin wound repair. In addition, the natural active ingredients biological polypeptides and stem cell exosomes have less irritation, lower drug side effects, and more significant efficacy. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 This is an overall flow chart of a method for preparing a biological cell polypeptide proposed by the present invention;

[0024] Figure 2 This is a flow chart of a method for preparing biological cell polypeptides proposed by the present invention, in which whey powder and fish meal are selected as preparation raw materials and the enzymatic hydrolysis time of the biological enzyme is 6 hours;

[0025] Figure 3 This is a flow chart of a method for preparing biological cell polypeptides proposed by the present invention, in which whey powder and fresh milk are selected as preparation raw materials and the enzymatic hydrolysis time of the biological enzyme is 6 hours;

[0026] Figure 4 This is a flow chart of a method for preparing biological cell polypeptides proposed by the present invention, in which fish meal and fresh milk are selected as preparation raw materials and the enzymatic hydrolysis time of the biological enzyme is 6 hours;

[0027] Figure 5 This is a flow chart of a method for preparing biological cell polypeptides proposed by the present invention, in which whey powder and fish meal are selected as preparation raw materials and the enzymatic hydrolysis time of biological enzymes is 7 hours;

[0028] Figure 6 This is a flow chart of a method for preparing biological cell polypeptides proposed by the present invention, in which whey powder and fresh milk are selected as preparation raw materials and the enzymatic hydrolysis time of the biological enzyme is 7 hours;

[0029] Figure 7 This is a flow chart of a method for preparing biological cell polypeptides proposed by the present invention, in which fish meal and fresh milk are selected as preparation raw materials and the enzymatic hydrolysis time of the biological enzyme is 7 hours;

[0030] Figure 8 This is a flow chart of a method for preparing biological cell polypeptides proposed by the present invention, in which whey powder and fish meal are selected as preparation raw materials and the enzymatic hydrolysis time of the biological enzyme is 8 hours;

[0031] Fig. 9 This is a flow chart of a method for preparing biological cell polypeptides proposed by the present invention, in which whey powder and fresh milk are selected as preparation raw materials and the enzymatic hydrolysis time of the biological enzyme is 8 hours;

[0032] Fig.10 The present invention provides a flow chart of a method for preparing biological cell polypeptides, in which fish meal and fresh milk are selected as preparation raw materials and the enzymatic hydrolysis time of biological enzymes is 8 hours. DETAILED DESCRIPTION

[0033] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0034] Embodiment 1:

[0035] Reference Figure 1 and 2

[0036] A method for preparing a biological cell polypeptide comprises the following steps:

[0037] S1: Prepare the laboratory;

[0038] S2: Preparation of biological cell polypeptides;

[0039] S3: selecting two of whey powder, fish meal, and fresh milk as raw materials for preparing biological cell polypeptides;

[0040] S4: selecting two of the prepared raw materials of the combination, crushing and mixing them, and then conveying the mixed materials;

[0041] S5: selecting a combination of trypsin, pepsin and chymosin as biological enzymes for preparing biological cell polypeptides, and performing enzyme dissolution and delivery;

[0042] S6: adding the two selected raw materials and the biological enzyme into a constant temperature oscillator for enzymolysis. After the enzymolysis is completed, the temperature is raised to 95° C., the enzyme is inactivated for 10 minutes, and the mixture is cooled to room temperature.

[0043] S7: adding the raw material into a tubular centrifuge device for centrifugation, and after centrifugation, performing oil phase, liquid phase and solid phase separation, and then filtering and decolorizing;

[0044] S8: Ultrafiltration is performed through an ultrafiltration membrane, and the obtained ultrafiltrate is freeze-dried to obtain a biological polypeptide dry powder, which is quantitatively packaged and stored at a low temperature for later use.

[0045] In a specific implementation, in S1: the laboratory can be selected from a chemical laboratory or a biological laboratory, and the level of the laboratory is at least a P2 laboratory, and the laboratory environment requires a constant range of temperature, humidity and pressure difference.

[0046] Furthermore, in S3: a combination of whey powder and fish meal is selected as a raw material for preparing biological cell polypeptides.

[0047] Furthermore, in S4: the selected raw materials of the combination of whey powder and fish meal are crushed and mixed, and then the mixed materials are transported.

[0048] Further, in S6: the thermostatic oscillator is kept at a constant temperature of 50° C., and the enzymatic hydrolysis is carried out in the thermostatic oscillator at 50° C. for 6 hours.

[0049] Further, in S7: the centrifugation temperature of the tubular centrifuge device is 2-6°C.

[0050] Embodiment 2:

[0051] Reference Figure 1 and 3

[0052] A method for preparing a biological cell polypeptide comprises the following steps:

[0053] S1: Prepare the laboratory;

[0054] S2: Preparation of biological cell polypeptides;

[0055] S3: selecting two of whey powder, fish meal, and fresh milk as raw materials for preparing biological cell polypeptides;

[0056] S4: selecting two of the prepared raw materials of the combination, crushing and mixing them, and then conveying the mixed materials;

[0057] S5: selecting a combination of trypsin, pepsin and chymosin as biological enzymes for preparing biological cell polypeptides, and performing enzyme dissolution and delivery;

[0058] S6: adding the two selected raw materials and the biological enzyme into a constant temperature oscillator for enzymolysis. After the enzymolysis is completed, the temperature is raised to 95° C., the enzyme is inactivated for 10 minutes, and the mixture is cooled to room temperature.

[0059] S7: adding the raw material into a tubular centrifuge device for centrifugation, and after centrifugation, performing oil phase, liquid phase and solid phase separation, and then filtering and decolorizing;

[0060] S8: Ultrafiltration is performed through an ultrafiltration membrane, and the obtained ultrafiltrate is freeze-dried to obtain a biological polypeptide dry powder, which is quantitatively packaged and stored at a low temperature for later use.

[0061] In a specific implementation, in S1: the laboratory can be selected from a chemical laboratory or a biological laboratory, and the level of the laboratory is at least a P2 laboratory, and the laboratory environment requires a constant range of temperature, humidity and pressure difference.

[0062] Furthermore, in S3: a combination of whey powder and fresh milk is selected as a raw material for preparing biological cell polypeptides.

[0063] Furthermore, in S4: the selected raw materials of the combination of whey powder and fresh milk are crushed and mixed, and then the mixed materials are transported.

[0064] Further, in S6: the thermostatic oscillator is kept at a constant temperature of 50° C., and the enzymatic hydrolysis is carried out in the thermostatic oscillator at 50° C. for 6 hours.

[0065] Further, in S7: the centrifugation temperature of the tubular centrifuge device is 2-6°C.

[0066] Embodiment three:

[0067] Reference Figure 1 and 4

[0068] A method for preparing a biological cell polypeptide comprises the following steps:

[0069] S1: Prepare the laboratory;

[0070] S2: Preparation of biological cell polypeptides;

[0071] S3: selecting two of whey powder, fish meal, and fresh milk as raw materials for preparing biological cell polypeptides;

[0072] S4: selecting two of the prepared raw materials of the combination, crushing and mixing them, and then conveying the mixed materials;

[0073] S5: selecting a combination of trypsin, pepsin and chymosin as biological enzymes for preparing biological cell polypeptides, and performing enzyme dissolution and delivery;

[0074] S6: adding the two selected raw materials and the biological enzyme into a constant temperature oscillator for enzymolysis. After the enzymolysis is completed, the temperature is raised to 95° C., the enzyme is inactivated for 10 minutes, and the mixture is cooled to room temperature.

[0075] S7: adding the raw material into a tubular centrifuge device for centrifugation, and after centrifugation, performing oil phase, liquid phase and solid phase separation, and then filtering and decolorizing;

[0076] S8: Ultrafiltration is performed through an ultrafiltration membrane, and the obtained ultrafiltrate is freeze-dried to obtain a biological polypeptide dry powder, which is quantitatively packaged and stored at a low temperature for later use.

[0077] In a specific implementation, in S1: the laboratory can be selected from a chemical laboratory or a biological laboratory, and the level of the laboratory is at least a P2 laboratory, and the laboratory environment requires a constant range of temperature, humidity and pressure difference.

[0078] Furthermore, in S3: a combination of fish meal and fresh milk is selected as a raw material for preparing biological cell polypeptides.

[0079] Furthermore, in S4: the selected raw materials of the combination of fish meal and fresh milk are crushed and mixed, and then the mixed materials are transported.

[0080] Further, in S6: the thermostatic oscillator is kept at a constant temperature of 50° C., and the enzymatic hydrolysis is carried out in the thermostatic oscillator at 50° C. for 6 hours.

[0081] Further, in S7: the centrifugation temperature of the tubular centrifuge device is 2-6°C.

[0082] Embodiment 4:

[0083] Reference Figure 1 and 5

[0084] A method for preparing a biological cell polypeptide comprises the following steps:

[0085] S1: Prepare the laboratory;

[0086] S2: Preparation of biological cell polypeptides;

[0087] S3: selecting two of whey powder, fish meal, and fresh milk as raw materials for preparing biological cell polypeptides;

[0088] S4: selecting two of the prepared raw materials of the combination, crushing and mixing them, and then conveying the mixed materials;

[0089] S5: selecting a combination of trypsin, pepsin and chymosin as biological enzymes for preparing biological cell polypeptides, and performing enzyme dissolution and delivery;

[0090] S6: adding the two selected raw materials and the biological enzyme into a constant temperature oscillator for enzymolysis. After the enzymolysis is completed, the temperature is raised to 95° C., the enzyme is inactivated for 10 minutes, and the mixture is cooled to room temperature.

[0091] S7: adding the raw material into a tubular centrifuge device for centrifugation, and after centrifugation, performing oil phase, liquid phase and solid phase separation, and then filtering and decolorizing;

[0092] S8: Ultrafiltration is performed through an ultrafiltration membrane, and the obtained ultrafiltrate is freeze-dried to obtain a biological polypeptide dry powder, which is quantitatively packaged and stored at a low temperature for later use.

[0093] In a specific implementation, in S1: the laboratory can be selected from a chemical laboratory or a biological laboratory, and the level of the laboratory is at least a P2 laboratory, and the laboratory environment requires a constant range of temperature, humidity and pressure difference.

[0094] Furthermore, in S3: a combination of whey powder and fish meal is selected as a raw material for preparing biological cell polypeptides.

[0095] Furthermore, in S4: the selected raw materials of the combination of whey powder and fish meal are crushed and mixed, and then the mixed materials are transported.

[0096] Further, in S6: the thermostatic oscillator is kept at a constant temperature of 50° C., and the enzymatic hydrolysis is performed in the thermostatic oscillator at 50° C. for 7 hours.

[0097] Further, in S7: the centrifugation temperature of the tubular centrifuge device is 2-6°C.

[0098] Embodiment five:

[0099] Reference Figure 1 and 6

[0100] A method for preparing a biological cell polypeptide comprises the following steps:

[0101] S1: Prepare the laboratory;

[0102] S2: Preparation of biological cell polypeptides;

[0103] S3: selecting two of whey powder, fish meal, and fresh milk as raw materials for preparing biological cell polypeptides;

[0104] S4: selecting two of the prepared raw materials of the combination, crushing and mixing them, and then conveying the mixed materials;

[0105] S5: selecting a combination of trypsin, pepsin and chymosin as biological enzymes for preparing biological cell polypeptides, and performing enzyme dissolution and delivery;

[0106] S6: adding the two selected raw materials and the biological enzyme into a constant temperature oscillator for enzymolysis. After the enzymolysis is completed, the temperature is raised to 95° C., the enzyme is inactivated for 10 minutes, and the mixture is cooled to room temperature.

[0107] S7: adding the raw material into a tubular centrifuge device for centrifugation, and after centrifugation, performing oil phase, liquid phase and solid phase separation, and then filtering and decolorizing;

[0108] S8: Ultrafiltration is performed through an ultrafiltration membrane, and the obtained ultrafiltrate is freeze-dried to obtain a biological polypeptide dry powder, which is quantitatively packaged and stored at a low temperature for later use.

[0109] In a specific implementation, in S1: the laboratory can be selected from a chemical laboratory or a biological laboratory, and the level of the laboratory is at least a P2 laboratory, and the laboratory environment requires a constant range of temperature, humidity and pressure difference.

[0110] Furthermore, in S3: a combination of whey powder and fresh milk is selected as a raw material for preparing biological cell polypeptides.

[0111] Furthermore, in S4: the selected raw materials of the combination of whey powder and fresh milk are crushed and mixed, and then the mixed materials are transported.

[0112] Further, in S6: the thermostatic oscillator is kept at a constant temperature of 50° C., and the enzymatic hydrolysis is carried out in the thermostatic oscillator at 50° C. for 7 hours.

[0113] Further, in S7: the centrifugation temperature of the tubular centrifuge device is 2-6°C.

[0114] Embodiment six:

[0115] Reference Figure 1 and 7

[0116] A method for preparing a biological cell polypeptide comprises the following steps:

[0117] S1: Prepare the laboratory;

[0118] S2: Preparation of biological cell polypeptides;

[0119] S3: selecting two of whey powder, fish meal, and fresh milk as raw materials for preparing biological cell polypeptides;

[0120] S4: selecting two of the prepared raw materials of the combination, crushing and mixing them, and then conveying the mixed materials;

[0121] S5: selecting a combination of trypsin, pepsin and chymosin as biological enzymes for preparing biological cell polypeptides, and performing enzyme dissolution and delivery;

[0122] S6: adding the two selected raw materials and the biological enzyme into a constant temperature oscillator for enzymolysis. After the enzymolysis is completed, the temperature is raised to 95° C., the enzyme is inactivated for 10 minutes, and the mixture is cooled to room temperature.

[0123] S7: adding the raw material into a tubular centrifuge device for centrifugation, and after centrifugation, performing oil phase, liquid phase and solid phase separation, and then filtering and decolorizing;

[0124] S8: Ultrafiltration is performed through an ultrafiltration membrane, and the obtained ultrafiltrate is freeze-dried to obtain a biological polypeptide dry powder, which is quantitatively packaged and stored at a low temperature for later use.

[0125] In a specific implementation, in S1: the laboratory can be selected from a chemical laboratory or a biological laboratory, and the level of the laboratory is at least a P2 laboratory, and the laboratory environment requires a constant range of temperature, humidity and pressure difference.

[0126] Furthermore, in S3: a combination of fish meal and fresh milk is selected as a raw material for preparing biological cell polypeptides.

[0127] Furthermore, in S4: the selected raw materials of the combination of fish meal and fresh milk are crushed and mixed, and then the mixed materials are transported.

[0128] Further, in S6: the thermostatic oscillator is kept at a constant temperature of 50° C., and the enzymatic hydrolysis is performed in the thermostatic oscillator at 50° C. for 7 hours.

[0129] Further, in S7: the centrifugation temperature of the tubular centrifuge device is 2-6°C.

[0130] Embodiment seven:

[0131] Reference Figure 1 and 8

[0132] A method for preparing a biological cell polypeptide comprises the following steps:

[0133] S1: Prepare the laboratory;

[0134] S2: Preparation of biological cell polypeptides;

[0135] S3: selecting two of whey powder, fish meal, and fresh milk as raw materials for preparing biological cell polypeptides;

[0136] S4: selecting two of the prepared raw materials of the combination, crushing and mixing them, and then conveying the mixed materials;

[0137] S5: selecting a combination of trypsin, pepsin and chymosin as biological enzymes for preparing biological cell polypeptides, and performing enzyme dissolution and delivery;

[0138] S6: adding the two selected raw materials and the biological enzyme into a constant temperature oscillator for enzymolysis. After the enzymolysis is completed, the temperature is raised to 95° C., the enzyme is inactivated for 10 minutes, and the mixture is cooled to room temperature.

[0139] S7: adding the raw material into a tubular centrifuge device for centrifugation, and after centrifugation, performing oil phase, liquid phase and solid phase separation, and then filtering and decolorizing;

[0140] S8: Ultrafiltration is performed through an ultrafiltration membrane, and the obtained ultrafiltrate is freeze-dried to obtain a biological polypeptide dry powder, which is quantitatively packaged and stored at a low temperature for later use.

[0141] In a specific implementation, in S1: the laboratory can be selected from a chemical laboratory or a biological laboratory, and the level of the laboratory is at least a P2 laboratory, and the laboratory environment requires a constant range of temperature, humidity and pressure difference.

[0142] Furthermore, in S3: a combination of whey powder and fish meal is selected as a raw material for preparing biological cell polypeptides.

[0143] Furthermore, in S4: the selected raw materials of the combination of whey powder and fish meal are crushed and mixed, and then the mixed materials are transported.

[0144] Further, in S6: the thermostatic oscillator is kept at a constant temperature of 50° C., and the enzymatic hydrolysis is performed in the thermostatic oscillator at 50° C. for 8 h.

[0145] Further, in S7: the centrifugation temperature of the tubular centrifuge device is 2-6°C.

[0146] Embodiment eight:

[0147] Reference Figure 1 and 9

[0148] A method for preparing a biological cell polypeptide comprises the following steps:

[0149] S1: Prepare the laboratory;

[0150] S2: Preparation of biological cell polypeptides;

[0151] S3: selecting two of whey powder, fish meal, and fresh milk as raw materials for preparing biological cell polypeptides;

[0152] S4: selecting two of the prepared raw materials of the combination, crushing and mixing them, and then conveying the mixed materials;

[0153] S5: selecting a combination of trypsin, pepsin and chymosin as biological enzymes for preparing biological cell polypeptides, and performing enzyme dissolution and delivery;

[0154] S6: adding the two selected raw materials and the biological enzyme into a constant temperature oscillator for enzymolysis. After the enzymolysis is completed, the temperature is raised to 95° C., the enzyme is inactivated for 10 minutes, and the mixture is cooled to room temperature.

[0155] S7: adding the raw material into a tubular centrifuge device for centrifugation, and after centrifugation, performing oil phase, liquid phase and solid phase separation, and then filtering and decolorizing;

[0156] S8: Ultrafiltration is performed through an ultrafiltration membrane, and the obtained ultrafiltrate is freeze-dried to obtain a biological polypeptide dry powder, which is quantitatively packaged and stored at a low temperature for later use.

[0157] In a specific implementation, in S1: the laboratory can be selected from a chemical laboratory or a biological laboratory, and the level of the laboratory is at least a P2 laboratory, and the laboratory environment requires a constant range of temperature, humidity and pressure difference.

[0158] Furthermore, in S3: a combination of whey powder and fresh milk is selected as a raw material for preparing biological cell polypeptides.

[0159] Furthermore, in S4: the selected raw materials of the combination of whey powder and fresh milk are crushed and mixed, and then the mixed materials are transported.

[0160] Further, in S6: the thermostatic oscillator is kept at a constant temperature of 50° C., and the enzymatic hydrolysis is performed in the thermostatic oscillator at 50° C. for 8 h.

[0161] Further, in S7: the centrifugation temperature of the tubular centrifuge device is 2-6°C.

[0162] Embodiment nine:

[0163] Reference Figure 1 and 10

[0164] A method for preparing a biological cell polypeptide comprises the following steps:

[0165] S1: Prepare the laboratory;

[0166] S2: Preparation of biological cell polypeptides;

[0167] S3: selecting two of whey powder, fish meal, and fresh milk as raw materials for preparing biological cell polypeptides;

[0168] S4: selecting two of the prepared raw materials of the combination, crushing and mixing them, and then conveying the mixed materials;

[0169] S5: selecting a combination of trypsin, pepsin and chymosin as biological enzymes for preparing biological cell polypeptides, and performing enzyme dissolution and delivery;

[0170] S6: adding the two selected raw materials and the biological enzyme into a constant temperature oscillator for enzymolysis. After the enzymolysis is completed, the temperature is raised to 95° C., the enzyme is inactivated for 10 minutes, and the mixture is cooled to room temperature.

[0171] S7: adding the raw material into a tubular centrifuge device for centrifugation, and after centrifugation, performing oil phase, liquid phase and solid phase separation, and then filtering and decolorizing;

[0172] S8: Ultrafiltration is performed through an ultrafiltration membrane, and the obtained ultrafiltrate is freeze-dried to obtain a biological polypeptide dry powder, which is quantitatively packaged and stored at a low temperature for later use.

[0173] In a specific implementation, in S1: the laboratory can be selected from a chemical laboratory or a biological laboratory, and the level of the laboratory is at least a P2 laboratory, and the laboratory environment requires a constant range of temperature, humidity and pressure difference.

[0174] Furthermore, in S3: a combination of fish meal and fresh milk is selected as a raw material for preparing biological cell polypeptides.

[0175] Furthermore, in S4: the selected raw materials of the combination of fish meal and fresh milk are crushed and mixed, and then the mixed materials are transported.

[0176] Further, in S6: the thermostatic oscillator is kept at a constant temperature of 50° C., and the enzymatic hydrolysis is performed in the thermostatic oscillator at 50° C. for 8 h.

[0177] Further, in S7: the centrifugation temperature of the tubular centrifuge device is 2-6°C.

[0178] In the present invention, two of whey powder, fish meal, and fresh milk are selected as raw materials for preparing biological cell polypeptides, and multiple samples are provided. After the preparation of the biological cell polypeptides is completed, it is convenient for the experimenters to compare and record, so that the experimenters can select two of the preparation raw materials among whey powder, fish meal, and fresh milk, and the selected biological enzymes of the combination of trypsin, pepsin, and chymosin are enzymolyzed in a constant temperature oscillator at 50°C for 6h, 7h, and 8h, so that the experimenters can observe the biological enzymes of the combination of trypsin, pepsin, and chymosin at 50°C. The enzymatic hydrolysis effects of 6h, 7h and 8h in the constant temperature oscillator are convenient for experimenters to compare the enzymatic hydrolysis effects at different times, so that experimenters can preferentially select the enzymatic hydrolysis time of the combination of trypsin, pepsin and rennet in the 50℃ constant temperature oscillator, so that the prepared biological cell polypeptide has the advantages of safety, stability, easy solubility in water, easy absorption, small molecular weight, etc., and at the same time has multiple biological activities such as scavenging free radicals, anti-oxidation, anti-aging, etc., which can not only promote the proliferation of skin cells, but also provide nutrition for the skin and delay skin aging.

[0179] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A method for preparing a biological cell polypeptide, characterized in that: The following steps are involved: S1: Prepare the laboratory; S2: Preparation of biological cell polypeptides; S3: selecting two of whey powder, fish meal, and fresh milk as raw materials for preparing biological cell polypeptides; S4: crushing and mixing the selected two combinations of the prepared raw materials, and then conveying the mixed materials; S5: selecting a combination of trypsin, pepsin and chymosin as biological enzymes for preparing biological cell polypeptides, and performing enzyme dissolution and delivery; S6: adding the two selected raw materials and the biological enzyme into a constant temperature oscillator for enzymolysis. After the enzymolysis is completed, the temperature is raised to 95° C., the enzyme is inactivated for 10 minutes, and the mixture is cooled to room temperature. S7: adding the raw material into a tubular centrifuge device for centrifugation, and after centrifugation, performing oil phase, liquid phase and solid phase separation, and then filtering and decolorizing; S8: Ultrafiltration is performed through an ultrafiltration membrane, and the obtained ultrafiltrate is freeze-dried to obtain a biological polypeptide dry powder, which is quantitatively packaged and stored at a low temperature for later use.

2. A method for preparing a biological cell polypeptide according to claim 1, characterized in that: In S1: the laboratory can be selected from a chemical laboratory or a biological laboratory, and the grade of the laboratory is at least a P2 laboratory, and the laboratory environment requires a constant range of temperature, humidity and pressure difference.

3. The method for preparing a biological cell polypeptide according to claim 1, characterized in that: In S3, two of the selected preparation raw materials among whey powder, fish meal, and fresh milk may be whey powder and fish meal, whey powder and fresh milk, or fish meal and fresh milk.

4. The method for preparing a biological cell polypeptide according to claim 1, characterized in that: In S4, the selected raw materials of the combination of whey powder and fish meal, whey powder and fresh milk, or fish meal and fresh milk are crushed and mixed, and then the mixed materials are transported.

5. The method for preparing a biological cell polypeptide according to claim 1, characterized in that: In S6: the thermostatic oscillator is kept at a constant temperature of 50° C., and enzymolysis is performed in the thermostatic oscillator at 50° C. for 6 hours.

6. The method for preparing a biological cell polypeptide according to claim 1, characterized in that: In S6: the thermostatic oscillator is kept at a constant temperature of 50° C., and the enzymolysis is carried out in the thermostatic oscillator at 50° C. for 7 hours.

7. The method for preparing a biological cell polypeptide according to claim 1, characterized in that: In S6: the thermostatic oscillator is kept at a constant temperature of 50° C., and the enzymatic hydrolysis is carried out in the thermostatic oscillator at 50° C. for 8 hours.

8. The method for preparing a biological cell polypeptide according to claim 1, characterized in that: In S7: the centrifugation temperature of the tubular centrifuge device is 2-6°C.

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