Pterocarpus santalinus rapid edgeworthia chrysantha promoter and method for rapid edgeworthia chrysantha

Abstract

The invention provides a rapid red sandalwood edgeworthia chrysantha promoter and a rapid red sandalwood edgeworthia chrysantha edgeworthia chrysantha edgeworthia chrysantha The invention provides a red sandalwood rapid edgeworthia chrysantha promoter which is prepared from the following raw materials in parts by weight: 2-3 parts of rice, 1-2 parts of herba ecliptae, 0.8-1.2 parts of red-knees herb and 0.8-1.2 parts of lemongrass. The preparation method of the red sandalwood rapid edgeworthia chrysantha promoter comprises the following steps: respectively weighing rice, eclipta, red-knees herb and lemongrass in parts by weight, mixing and crushing, adding water according to a material-liquid ratio g / ml of 1: (1-2), placing in a sealed fermentation tank, sealing and fermenting for 30-40 days, filtering to obtain a fermentation liquid, adding a composite strain, and fermenting for 30-40 days to obtain the red sandalwood rapid edgeworthia chrysantha promoter. The preparation method comprises the following steps: uniformly mixing a fermentation strain consisting of Bosella thiooxidans, Verticillium theobromae and Bacillus pasteurii, carrying out sealed fermentation for 7-8 days, filtering, and taking a filtrate, so as to obtain the rapid Pterocarpus santalinus Edgeworthia chrysantha Edgeworthia chrysantha (L.) DC. After the method is adopted for treatment, formation of the red sandalwood heartwood is effectively accelerated and promoted, and meanwhile the quality of the red sandalwood heartwood is effectively improved.

Description

Technical Field

[0001] The invention relates to the field of plant planting, and in particular to a red sandalwood rapid fragrance-forming promoter and a red sandalwood rapid fragrance-forming method. Background Art

[0002] Rosewood is one of the important and precious timber species in my country, and its heartwood has important applications in medicine, furniture making and other industries. Therefore, the formation of rosewood heartwood has a significant impact on its value. Existing studies have shown that the essential oil content of perennial rosewood heartwood ranges from about 1.5% to 1.8%, and alcohols are the category with the highest content in the heartwood essential oil components, followed by alkanes, among which trans-nerolidol has a higher value, with a content of about 7.5%. Rosewood can form heartwood naturally, and the heartwood essential oil contains a variety of precious medicinal ingredients, but the speed of heartwood formation is very slow. Traditional rosewood incense-making technology usually relies on natural conditions and environmental factors. Therefore, it is urgent to develop a fast and efficient rosewood incense-making method to accelerate the formation of rosewood heartwood. Summary of the invention

[0003] In view of this, the present invention proposes a method for quickly forming red sandalwood incense.

[0004] The technical solution of the present invention is achieved in this way:

[0005] The invention provides a red sandalwood rapid fragrance-forming promoter, which is prepared from the following raw materials in parts by weight: 2-3 parts of rice, 1-2 parts of Eclipta prostrata, 0.8-1.2 parts of Polygonum hydropiper, and 0.8-1.2 parts of citronella.

[0006] A further technical solution is that the red sandalwood rapid fragrance-forming promoter is prepared by natural fermentation and bacterial fermentation of rice, Eclipta prostrata, Polygonum hydropiper and Citronella cymoides.

[0007] A further technical solution is that the preparation method of the red sandalwood rapid aroma-forming promoter comprises the following steps: respectively weighing rice, Eclipta prostrata, Polygonum hydropiper, and Citronella by weight, mixing and crushing, adding water at a solid-liquid ratio of 1:1-2 g / ml, placing in a sealed fermentation tank, sealing and naturally fermenting for 30-40 days, filtering to obtain fermentation liquid, adding composite bacteria, uniformly mixing, sealing and fermenting for 7-8 days, filtering, and taking the filtrate to obtain the red sandalwood rapid aroma-forming promoter.

[0008] A further technical solution is that the composite bacteria are composed of Bosea thiooxidans, Musicillium theobromae and Sporosarcina pasteurii; the ratio of the fermentation liquid to Bosea thiooxidans, Musicillium theobromae and Sporosarcina pasteurii is 1000ml:1.5-2g:0.4-0.6g:0.8-1.2g.

[0009] A further technical solution is that the fermentation temperature is 30-40°C.

[0010] The present invention also provides a method for rapid fragrance formation of red sandalwood, comprising the following steps: selecting a red sandalwood tree, and using a drill bit to perform transverse drilling on the trunk along the horizontal direction of the cross-sectional diameter of the tree, wherein the depth of the transverse drilling hole is 60% to 70% of the trunk diameter; within 15 minutes after the drilling, drip-injecting any red sandalwood rapid fragrance formation promoter described in the present invention into the hole in the form of infusion, wherein the injection amount of the red sandalwood rapid fragrance formation promoter is 250 to 300 ml.

[0011] A further technical solution is that the dripping rate is 15 to 20 drops per minute.

[0012] A further technical solution is that the drilling hole is located 15 to 25 cm above the ground.

[0013] A further technical solution is that the drill bit diameter is 3.5 to 4.5 mm.

[0014] A further technical solution is that the red sandalwood tree has a trunk diameter of more than 15 cm.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] (1) The red sandalwood rapid aroma-forming promoter of the present invention is prepared by natural fermentation and bacterial fermentation of rice, Eclipta prostrata, Polygonum hydropiper, and Citronella cymoides. Bosella sulfoxidans, Verticillium theobroma, and Bacillus pasteurianum are added during bacterial fermentation. After being treated with the red sandalwood rapid aroma-forming promoter of the present invention, the formation of red sandalwood heartwood is effectively accelerated, and the quality of the red sandalwood heartwood is effectively improved. The ethanol extract content, volatile oil yield, and trans-nerolidol content in the volatile oil of the heartwood formed by the present invention are significantly improved.

[0017] (2) The present invention targets red sandalwood and uses Bosella sulfuroxidans, Verticillium theobroma and Bacillus pasteurianum to ferment. The red sandalwood rapid aroma promoter obtained by fermentation can better promote the accumulation of lipid substances in the xylem, accelerate the formation of red sandalwood heartwood, and effectively improve the quality of red sandalwood heartwood. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a schematic diagram of the punching method of red sandalwood of the present invention.

[0019] Figure 2 The following are photos of the heartwood of red sandalwood. In the photo, the left side shows the heartwood formed by the treatment of Example 1 of the present invention; the right side shows the heartwood formed naturally (the middle part is the heartwood). DETAILED DESCRIPTION

[0020] In order to better understand the technical content of the present invention, specific embodiments are provided below to further illustrate the present invention.

[0021] Unless otherwise specified, the experimental methods used in the embodiments of the present invention are all conventional methods.

[0022] Unless otherwise specified, the materials, reagents, etc. used in the embodiments of the present invention can be obtained from commercial sources.

[0023] Bosea thiooxidans, Latin name Bosea thiooxidans, was purchased from China General Microbiological Culture Collection Center, with the strain number CGMCC NO.1.9081.

[0024] Musicillium theobromae, Latin name Musicillium theobromae, was purchased from Guangdong Provincial Culture Collection Management Center, with the strain number GDMCC NO.3.519.

[0025] Bacillus pasteurianus, with the Latin name Sporosarcina pasteurii, was purchased from Guangdong Provincial Culture Collection Management Center with the strain number GDMCC NO.1.803.

[0026] The Bosella sulfuroxidans, Verticillium theobroma and Bacillus pasteurianum used in the examples of the present invention and the comparative examples are all bacterial powders, and the live bacteria concentrations thereof are all ≥ 100 million CFU / g.

[0027] Preparation Example 1-Red Sandalwood Rapid Fragrance Accelerator

[0028] The invention discloses a red sandalwood quick fragrance-forming promoter, which is prepared from the following raw materials in the following mass proportions: 2.5 kg of rice, 1.5 kg of Eclipta prostrata, 1.0 kg of Polygonum hydropiper, and 1.0 kg of citronella.

[0029] Preparation method: according to the above weight proportion, take rice, Eclipta prostrata, Polygonum hydropiper, and citronella, grind them, add purified water at a solid-liquid ratio of 1:2, place them in a sealed fermentation tank, and seal and naturally ferment at 35°C±2°C for 35 days; take out and filter, take the filtrate and place it in a sterile brown glass bottle or other opaque container to obtain a fermentation liquid; take 1000 ml of the fermentation liquid, add 1.8g of Bosella sulfuroxidans, 0.5g of Verticillium theobroma and 1.0g of Bacillus pasteurianum, mix them evenly, and then seal and ferment at 35°C±2°C for 7 days, take the filtrate, and prepare a red sandalwood rapid aroma promoter.

[0030] Preparation Example 2-Red Sandalwood Rapid Fragrance Accelerator

[0031] The invention discloses a red sandalwood quick fragrance-forming promoter, which is prepared from the following raw materials in the following mass proportions: 2.0 kg of rice, 2.0 kg of Eclipta prostrata, 1.2 kg of Polygonum hydropiper, and 0.8 kg of citronella.

[0032] Preparation method: according to the above weight proportion, take rice, Eclipta prostrata, Polygonum hydropiper, and citronella, grind them, add purified water at a solid-liquid ratio of 1:2, place them in a sealed fermentation tank, and seal and naturally ferment at 35°C±2°C for 35 days; take out and filter, take the filtrate and place it in a sterile brown glass bottle or other opaque container to obtain a fermentation liquid; take 1000 ml of the fermentation liquid, add 1.5g of Bosella sulfuroxidans, 0.6g of Verticillium cocoae and 1.2g of Bacillus pasteurianum, mix them evenly, and then seal and ferment at 35°C±2°C for 7 days, take the filtrate, and prepare a red sandalwood rapid aroma promoter.

[0033] Preparation Example 3-Red Sandalwood Rapid Fragrance Accelerator

[0034] The invention discloses a red sandalwood quick fragrance-forming promoter, which is prepared from the following raw materials in the following mass proportions: 3.0 kg of rice, 1.0 kg of Eclipta prostrata, 0.8 kg of Polygonum hydropiper, and 1.2 kg of citronella.

[0035] Preparation method: according to the above weight proportion, take rice, Eclipta prostrata, Polygonum hydropiper, and citronella, grind them, add purified water at a solid-liquid ratio of 1:2, place them in a sealed fermentation tank, and seal and naturally ferment at 35°C±2°C for 35 days; take out and filter, take the filtrate and place it in a sterile brown glass bottle or other opaque container to obtain a fermentation liquid; take 1000 ml of the fermentation liquid, add 2.0g of Bosella sulfuroxidans, 0.4g of Verticillium cocoae and 0.9g of Bacillus pasteurianum, mix them evenly, and then seal and ferment at 35°C±2°C for 7 days, take the filtrate to prepare a red sandalwood rapid aroma promoter.

[0036] Example 1 - Method for Rapidly Forming Red Sandalwood Incense (Preparation Example 1 Red Sandalwood Rapid Incense Formation Accelerator)

[0037] Select a red sandalwood tree with a trunk diameter of about 15 cm, and use a 4mm drill bit to drill a horizontal hole in the trunk along the horizontal direction of its cross-sectional diameter at a height of 15 cm from the ground. The hole depth should be two-thirds of the trunk; within 15 minutes after drilling, begin to drip-inject the red sandalwood rapid fragrance promoter prepared in Preparation Example 1 into the hole in the form of an infusion. The total amount of liquid injected is 280 ml, and the dripping rate is 15 drops per minute. Seven days after the solution is completely injected into the tree, water it in time according to weather conditions, and apply a small amount of fertilizer to ensure that the plant has sufficient nutrients and water. The heartwood can be collected one year after the tree is treated. The longer the tree is, the more heartwood is formed and the better the quality. The drilling method is as follows: Figure 1 The photo of the heartwood of red sandalwood is shown in Figure 2 As shown (manual processing).

[0038] The proportion of heartwood formed naturally in perennial red sandalwood is about 25% (natural heartwood), and the proportion of heartwood formed after treatment by the method of Example 1 of the present invention exceeds 60% (artificial treatment). The red sandalwood rapid fragrance-forming promoter prepared by the present invention accelerates the formation of red sandalwood heartwood and effectively improves the quality of red sandalwood heartwood.

[0039] Example 2 - Method for Rapidly Forming Red Sandalwood Incense (Preparation Example 2 Red Sandalwood Rapid Incense Formation Accelerator)

[0040] Select a red sandalwood tree with a trunk diameter of about 15 cm, and use a 4mm drill bit to drill a horizontal hole in the trunk along the horizontal direction of its cross-sectional diameter at a height of 15 cm from the ground, with a hole depth of two-thirds of the trunk; within 15 minutes after drilling, start dripping the red sandalwood rapid fragrance promoter of Preparation Example 2 into the hole in the form of infusion, with a total amount of 280 ml of injected liquid and a drip rate of 15 drops per minute. Seven days after the solution is completely injected into the tree, water can be applied in time according to weather conditions, and a small amount of fertilizer can be applied to ensure that the plant has sufficient nutrients and water. The heartwood can be collected after the tree is treated for one year. The longer the age, the more heartwood is formed and the better the quality.

[0041] Example 3 - Method for Rapidly Forming Red Sandalwood Incense (Preparation Example 3 Red Sandalwood Rapid Incense Formation Accelerator)

[0042] Select a red sandalwood tree with a trunk diameter of about 15 cm, and use a 4mm drill bit to drill a horizontal hole in the trunk along the horizontal direction of its cross-sectional diameter at a height of 15 cm from the ground, with a hole depth of two-thirds of the trunk; within 15 minutes after drilling, start dripping the red sandalwood rapid fragrance promoter of Preparation Example 3 into the hole in the form of infusion, with a total amount of 280 ml of injected liquid and a drip rate of 15 drops per minute. After the solution is completely injected into the tree body for 7 days, water can be applied in time according to weather conditions, and a small amount of fertilizer can be applied to ensure that the plant has sufficient nutrients and water. The heartwood can be collected after the tree body is treated for 1 year. The longer the age, the more heartwood is formed and the better the quality.

[0043] Comparative Example 1

[0044] The main difference from Example 1 is that the formula components of the red sandalwood rapid fragrance-forming promoter are different, citronella is not included, and the amount of Polygonum hydropiper is increased by an equal amount. By weight ratio, the following raw materials are prepared: 2.5 kg of rice, 1.5 kg of Eclipta prostrata, and 2.0 kg of Polygonum hydropiper. Other treatments are the same as in Example 1.

[0045] Comparative Example 2

[0046] The main difference from Example 1 is that the formula components of the red sandalwood rapid fragrance-forming promoter are different, Polygonum hydropiper is not included, and the amount of citronella is increased by an equal amount. By weight ratio, the following raw materials are prepared: 2.5 kg of rice, 1.5 kg of Eclipta prostrata, and 2.0 kg of citronella. Other treatments are the same as in Example 1.

[0047] Comparative Example 3

[0048] The main difference from Example 1 is that the formula components of the red sandalwood rapid fragrance-forming promoter are different, and Eclipta prostrata is not included, and the amount of citronella and Polygonum hydropiper is increased accordingly. By weight ratio, the following raw materials are prepared: 2.5 kg of rice, 1.5 kg of Polygonum hydropiper, and 2.0 kg of citronella. Other treatments are consistent with Example 1.

[0049] Comparative Example 4

[0050] The main difference from Example 1 is that only the strain of Bosnia oxysporum is added during the preparation of the red sandalwood rapid aroma-forming promoter, and the amount of theobroma cacao is increased by an equal amount. According to the above weight ratio, rice, Eclipta prostrata, Polygonum hydropiper, and citronella are taken, crushed, and purified water is added according to a solid-liquid ratio of 1:2 g / ml, placed in a sealed fermentation tank, and sealed and naturally fermented at 35°C±2°C for 35d; taken out and filtered, the filtrate is placed in a sterile brown glass bottle or other light-proof container for storage to obtain a fermentation liquid; 1000 ml of the fermentation liquid is taken, 2.3g of theobroma cacao and 1.0g of Bacillus pasteurianus are added, and the mixture is evenly mixed, and then sealed and fermented at 35°C±2°C for 7d, and the filtrate is taken to obtain the red sandalwood rapid aroma-forming promoter.

[0051] The other treatments were the same as in Example 1.

[0052] Comparative Example 5

[0053] The main difference from Example 1 is that only the cocoa verticillium spores are added during the preparation of the red sandalwood rapid aroma-forming promoter, and the amount of Bacillus pasteurianus is increased by an equal amount. According to the above weight ratio, rice, Eclipta prostrata, Polygonum hydropiper, and citronella are taken and crushed, and purified water is added according to a solid-liquid ratio of 1:2 g / ml, and placed in a sealed fermentation tank, sealed and naturally fermented at 35°C±2°C for 35 days; taken out and filtered, the filtrate is placed in a sterile brown glass bottle or other light-proof container for storage to obtain a fermentation liquid; 1000 ml of the fermentation liquid is taken, 1.8g of Bosella sulfoxidans and 1.5g of Bacillus pasteurianus are added, and the mixture is evenly mixed, and then sealed and fermented at 35°C±2°C for 7 days, and the filtrate is taken to obtain the red sandalwood rapid aroma-forming promoter.

[0054] The other treatments were the same as in Example 1.

[0055] Comparative Example 6

[0056] The main difference from Example 1 is that only Bacillus pasteurianus is added during the preparation of the red sandalwood rapid aroma-forming promoter, and the amount of cocoa verticillium is increased by an equal amount. According to the above weight ratio, rice, Eclipta prostrata, Polygonum hydropiper, and citronella are taken and crushed, and purified water is added according to a solid-liquid ratio of 1:2 g / ml, and placed in a sealed fermentation tank, sealed and naturally fermented at 35°C±2°C for 35 days; taken out and filtered, the filtrate is placed in a sterile brown glass bottle or other light-proof container for storage to obtain a fermentation liquid; 1000 ml of the fermentation liquid is taken, 1.8g of Bosella sulfoxidans and 1.5g of cocoa verticillium are added, and the mixture is evenly mixed, and then sealed and fermented at 35°C±2°C for 7 days, and the filtrate is taken to obtain the red sandalwood rapid aroma-forming promoter.

[0057] The other treatments were the same as in Example 1.

[0058] Comparative Example 7

[0059] The main difference from Example 1 is that in the preparation process of the red sandalwood rapid aroma-forming promoter, only Bosella sulfuroxidans, Verticillium theobroma and Bacillus pasteurianum are added in a mass ratio of 1:1:1.

[0060] Preparation method of red sandalwood rapid aroma-forming accelerator: according to the above weight proportion, take 2.5kg of rice, 1.5kg of Eclipta prostrata, 1.0kg of Polygonum hydropiper, and 1.0kg of citronella, grind them, add purified water at a solid-liquid ratio of 1:2, place them in a sealed fermentation tank, and seal and naturally ferment at 35℃±2℃ for 35d; take out and filter, take the filtrate and place it in a sterile brown glass bottle or other light-proof container for storage to obtain fermentation liquid; take 1000ml of fermentation liquid, add 1.1g of Bosella sulfuroxidans, 1.1g of Verticillium cocoae and 1.1g of Bacillus pasteurianum, mix them evenly, and then seal and ferment at 35℃±2℃ for 7d, then take the filtrate to prepare the red sandalwood rapid aroma-forming accelerator.

[0061] Comparison example

[0062] The main difference from Example 1 is that the red sandalwood quick fragrance-forming promoter is not injected, and an equal volume of tap water is used instead. Other treatments are consistent with Example 1.

[0063] The test was carried out according to the methods of Examples 1-3 and Comparative Examples 1-7, and the test variety was red sandalwood. The trunk diameter of the red sandalwood plant was about 15 cm before the test. The heartwood was collected one year after the tree was treated, and the quality of the heartwood was tested. The detection method was: the hot soaking method and the volatile oil determination method in the alcohol-soluble extract determination method specified in the Pharmacopoeia of the People's Republic of China (2020 edition) were used to detect the ethanol extract content and volatile oil yield of the heartwood, and the HPLC method was used to detect the trans-nerolidol content in the volatile oil. The results are shown in Table 1.

[0064] Table 1 Comparison of rosewood heartwood quality

[0065]

[0066]

[0067] It can be seen from the results in the above table that the ethanol extract content of the heartwood formed by red sandalwood in Examples 1-3 is 26.17%-28.83%, the volatile oil yield is 2.81%-2.90%, and the trans-nerolidol content in the volatile oil is 20.48%-22.07%, which are significantly improved compared with the control example and comparative examples 1-7.

[0068] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A red sandalwood rapid fragrance-forming accelerator, characterized in that: The invention comprises the following raw materials by weight: 2-3 parts of rice, 1-2 parts of Eclipta prostrata, 0.8-1.2 parts of Polygonum hydropiper, and 0.8-1.2 parts of Cymbopogon citratus. The red sandalwood rapid fragrance-forming promoter is prepared by natural fermentation and bacterial fermentation of rice, Eclipta prostrata, Polygonum hydropiper, and Cymbopogon citratus.

2. The red sandalwood rapid fragrance-forming promoter according to claim 1, characterized in that The fermentation bacteria are composed of Bosea thiooxidans, Musicillium theobromae and Sporosarcina pasteurii.

3. The red sandalwood rapid fragrance-forming promoter according to claim 1, characterized in that The preparation method of the red sandalwood rapid aroma-forming promoter comprises the following steps: respectively weighing rice, Eclipta prostrata, Polygonum hydropiper, and Cymbopogon citratus by weight, mixing and crushing, adding water at a solid-liquid ratio of 1:1-2 (g / ml), placing in a sealed fermentation tank, sealing and naturally fermenting for 30-40 days, filtering to obtain fermentation liquid, adding composite bacteria, uniformly mixing, sealing and fermenting for 7-8 days, filtering, and taking the filtrate to obtain the red sandalwood rapid aroma-forming promoter.

4. The red sandalwood rapid fragrance-forming promoter according to claim 2, characterized in that The ratio of the fermentation liquid to Bosteria sulfuroxidans, Verticillium theobroma and Bacillus pasteurianum is 1000ml:1.5-2g:0.4-0.6g:0.8-1.2g.

5. The red sandalwood rapid fragrance-forming promoter according to claim 1, characterized in that The fermentation temperature is 30-40°C.

6. A method for rapidly forming red sandalwood incense, characterized in that: The following steps are involved: A red sandalwood tree is selected, and a transverse hole is drilled in the trunk horizontally along the cross-sectional diameter thereof using a drill bit, wherein the depth of the transverse hole is 60% to 70% of the trunk diameter; within 15 minutes after the drilling, the red sandalwood rapid aroma-forming promoter according to any one of claims 1 to 5 is drip-injected into the hole in the form of an infusion, wherein the injection amount of the red sandalwood rapid aroma-forming promoter is 250 to 300 ml.

7. The method for rapidly forming red sandalwood incense according to claim 6, characterized in that: The drip rate is 15 to 20 drops per minute.

8. The method for rapidly forming red sandalwood incense according to claim 6, characterized in that: The drilling position is 15 to 25 cm above the ground.

9. The method for rapidly forming red sandalwood incense according to claim 6, characterized in that: The drill bit diameter is 3.5~4.5mm.

10. The method for rapidly forming red sandalwood incense according to any one of claims 6 to 9, characterized in that: The red sandalwood tree is a red sandalwood tree with a trunk diameter of more than 15 cm.

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