Integrated bag for separating and collecting stem cells

Abstract

The invention belongs to the technical field of biomedicine, and discloses an integrated bag for stem cell separation and collection, the integrated bag comprises a bag body, one side of the bag body is fixedly connected with a support block, and the inside of the support block is movably connected with a drum assembly. Through cooperation of structures such as a rotating cylinder assembly and a moving block, the problem that external air or dust may enter the bag body through a gap when materials are injected is solved, a push plate moves towards the interior of a supporting block, then a convex rod slides along the track of an arc-shaped groove, and two gaskets are driven by the moving block to move oppositely, so that the materials are injected into the bag body. The sealing of the needle hole is relieved, then the needle tip is inserted into the circular groove of the bag body, injection is conducted on the interior of the circular groove, external impurities are intercepted by the push plate while the needle tube conducts injection, the external impurities are prevented from entering the bag body through the needle hole, and then the safety of stem cells is guaranteed.

Description

Technical Field

[0001] The invention belongs to the field of biomedical technology, and in particular is an integrated bag for separating and collecting stem cells. Background Art

[0002] The integrated stem cell separation and collection bag is a device used to separate and collect stem cells. The bag is usually made of special materials and has high air permeability and biocompatibility. It can effectively separate stem cells and concentrate them in a certain area. The integrated stem cell separation and collection bag is widely used in the field of biomedical research and can be used in stem cell therapy, regenerative medicine and tissue engineering.

[0003] When injecting growth factors or other materials into the existing one-piece bag, the sealing port or pipeline needs to be opened, and then the needle tube needs to be inserted to inject into the inside of the one-piece bag. However, during the injection process, external contaminants may enter the inside of the one-piece bag through the gap due to reasons such as loose sealing, thereby affecting the quality of stem cells and growth factors. Therefore, an integrated bag for stem cell separation and collection is proposed to solve the problems raised in the background technology. Summary of the invention

[0004] In order to solve the problem raised in the above background technology that external impurities may enter the inside of the integrated bag through the gap when injecting growth factors, thereby affecting the quality of stem cells, the present invention provides an integrated bag for stem cell separation and collection, which solves the problem that external air or dust may enter the inside of the bag body through the gap when injecting materials by setting the cooperation of structures such as a rotating drum assembly and a moving block, and the push plate moves toward the inside of the support block, so that the protrusion drives the cylindrical cam and the turntable to rotate along the trajectory of the first guide groove, and then the protruding rod slides along the trajectory of the arc groove, and drives the two gaskets to move in opposite directions through the moving block, thereby releasing the seal of the needle hole, and then the needle tip is inserted into the inside of the circular groove of the bag body, and the inside is injected. While the needle tube is injecting, the push plate intercepts external impurities to prevent them from entering the inside of the bag body through the pinhole, thereby ensuring the safety of the stem cells.

[0005] To achieve the above object, the present invention provides the following technical solution: an integrated bag for separation and collection of stem cells, comprising a bag body, a support block fixedly connected to one side of the bag body, and a rotating drum assembly movably connected inside the support block; Wherein, the drum assembly comprises a cylindrical cam, and a first guide groove is formed on the inner wall of the cylindrical cam at an angle of 180 degrees; The cylindrical cam is movably connected to a push plate inside, and the end of the cylindrical cam away from the push plate is fixedly connected to a turntable, the surface of the turntable is provided with an arc groove in a ring shape of 180 degrees, the inner ring shape of the support block is movably connected to a moving block in a ring shape of 180 degrees, and gaskets are fixedly connected to the opposite sides of the two moving blocks, and two pipes are mirrored on the top of the bag body, and a sealing assembly is movably connected to the inner part of the pipes, and the opposite ends of the two pipes are movably connected to a rotating rod, and the inner wall of the rotating rod is provided with a second guide groove in a ring shape of 180 degrees.

[0006] Preferably, the outer wall of the push plate is provided with a protrusion in an annular shape of 180 degrees, the first guide groove consists of an oblique groove with an arc of 90 degrees and a vertical groove with an arc of zero degrees, and the protrusion is movably engaged with the top of the oblique groove in the initial state.

[0007] Preferably, the cylindrical cam is movably connected to a support plate inside, and the bottom of the push plate is fixedly connected to a spring fixed to the support plate, and the spring is used to squeeze the push plate and keep it moving toward the outside of the support block.

[0008] Preferably, a convex rod is fixedly connected to one side of the moving block close to the turntable, and the convex rod is movably engaged in the inside of the arc groove. When the push plate moves toward the inside of the support block, it drives the cylindrical cam to rotate through the first guide groove. When the cylindrical cam rotates, it drives the turntable to rotate and makes the two convex rods move in opposite directions along the trajectory of the arc groove.

[0009] Preferably, the push plate and the middle of the turntable surface are both provided with pinholes, the gasket is made of rubber material, and the two gaskets are in abutment with each other in an initial state to seal the pinholes on the turntable.

[0010] Preferably, the inner wall of the bag body is provided with a slide groove cooperating with the moving block, the slide groove is used for circumferentially limiting the moving block when the turntable rotates, and the outer wall of the bag body is provided with a circular groove cooperating with the pinhole on the turntable.

[0011] Preferably, the sealing assembly comprises a moving rod, a round rod is provided at one end of the moving rod close to the rotating rod in an annular manner of 180 degrees, and a ventilation groove is provided in the middle of the moving rod.

[0012] Preferably, the arc of the second guide groove is 180 degrees, and the two round rods are movably connected inside the two second guide grooves respectively. When the rotating rod rotates, the round rod slides along the track of the second guide groove and drives the moving rod to move in a direction away from the rotating rod.

[0013] Preferably, the pipeline is cross-shaped, the diameter of the ventilation groove is the same as the inner diameter of the upper and lower ends of the pipeline, and the moving rod seals the middle part of the pipeline in the initial state.

[0014] Preferably, a block is provided at one end of the moving rod away from the round rod, and a straight slot matching the block is provided on the inner wall of the pipe, and the straight slot is used for circumferentially limiting the moving rod when the rotating rod rotates.

[0015] Compared with the prior art, the present invention has the following beneficial effects: The present invention solves the problem that external air or dust may enter the interior of the bag body through the gap when injecting materials by arranging the cooperation of structures such as the rotating drum assembly and the moving block. The push plate moves toward the inside of the support block, so that the convex block drives the cylindrical cam and the turntable to rotate along the trajectory of the first guide groove, and then the convex rod slides along the trajectory of the arc groove, and the two gaskets are driven to move in opposite directions by the moving block, thereby releasing the seal of the needle hole, and then the needle tip is inserted into the interior of the circular groove of the bag body, and the interior is injected. While the needle tube is injecting, the push plate intercepts external impurities to prevent them from entering the interior of the bag body through the pinhole, thereby ensuring the safety of stem cells.

[0016] The present invention facilitates the connection between the pipeline and the collection tube by arranging the cooperation of structures such as the sealing component and the rotating rod, so as to extract the stem cells in the bag body. By rotating the rotating rod, the round rod slides along the trajectory of the second guide groove, thereby driving the moving rod to move in the direction away from the rotating rod. Subsequently, the moving rod is limited by the straight groove, so that the moving rod drives the ventilation groove to overlap with the middle part of the pipeline, thereby releasing the seal of the pipeline, and facilitating the connection between the collection tube and the inside of the bag body. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the overall structure of the present invention; Figure 2 It is a schematic diagram of the side section structure of the rotary drum assembly of the present invention; Figure 3 It is a schematic diagram of the side section structure of the support block of the present invention; Figure 4 for Figure 3 Enlarged view of point A in the middle; Figure 5 It is a schematic diagram of the position relationship among the rotating disk, the moving block and the convex rod of the present invention; Figure 6 It is a schematic diagram of the cross-sectional structure of the pipeline of the present invention; Figure 7 It is a partial enlarged cross-sectional view of the rotating rod of the present invention.

[0018] In the figure: 1. bag body; 2. support block; 3. drum assembly; 301. cylindrical cam; 302. first guide groove; 303. support plate; 304. spring; 4. push plate; 5. turntable; 6. arc groove; 7. moving block; 8. convex rod; 9. gasket; 10. slide groove; 11. pipeline; 12. sealing assembly; 1201. moving rod; 1202. round rod; 1203. ventilation groove; 13. rotating rod; 14. second guide groove; 15. straight groove. DETAILED DESCRIPTION

[0019] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0020] like Figure 1 to Figure 1 As shown, the present invention provides an integrated bag for stem cell separation and collection, comprising a bag body 1, a support block 2 is fixedly connected to one side of the bag body 1, and a rotating drum assembly 3 is movably connected inside the support block 2; The drum assembly 3 includes a cylindrical cam 301, and a first guide groove 302 is formed on the inner wall of the cylindrical cam 301 at an angle of 180 degrees. The cylindrical cam 301 is movably connected to a push plate 4 inside, and a turntable 5 is fixedly connected to one end of the cylindrical cam 301 away from the push plate 4. An arc groove 6 is provided on the surface of the turntable 5 in a ring shape of 180 degrees. A moving block 7 is movably connected to the inner ring shape of the support block 2 at 180 degrees. Gaskets 9 are fixedly connected to the opposite sides of the two moving blocks 7. Two pipes 11 are mirror-imaged on the top of the bag body 1. A sealing assembly 12 is movably connected to the inner part of the pipe 11. A rotating rod 13 is movably connected to the opposite ends of the two pipes 11. A second guide groove 14 is provided on the inner wall of the rotating rod 13 in a ring shape of 180 degrees.

[0021] The above scheme is adopted: the push plate 4 is moved toward the inside of the support block 2, so that the convex block drives the cylindrical cam 301 and the turntable 5 to rotate along the trajectory of the first guide groove 302, and then the convex rod 8 slides along the trajectory of the arc groove 6, and drives the two gaskets 9 to move in the opposite direction through the moving block 7, thereby releasing the seal of the needle hole, and then the needle tip is inserted into the inside of the circular groove of the bag body 1, and the inside is injected. While the needle tube is injecting, the push plate 4 intercepts external impurities to prevent them from entering the inside of the bag body 1 through the needle hole, thereby ensuring the safety of stem cells.

[0022] like Figures 1 to 4As shown, the outer wall of the push plate 4 is provided with a protrusion in an annular shape of 180 degrees, and the first guide groove 302 is composed of an inclined groove with an arc of 90 degrees and a vertical groove with an arc of zero degrees. The protrusion is movably engaged with the top of the inclined groove in the initial state, and the inner movability of the cylindrical cam 301 is connected to the support plate 303, and the bottom of the push plate 4 is fixedly connected to a spring 304 fixedly connected to the support plate 303, and the spring 304 is used to squeeze the push plate 4 and keep it moving toward the outside of the support block 2.

[0023] The above scheme is adopted: the push plate 4 is supported by the spring 304, and its function is that when the growth factor injection is completed, the operator takes out the needle tube, thereby releasing the pressure on the push plate 4, so that the push plate 4 moves up under the action of the elastic force of the spring 304. While the push plate 4 moves up, the cylindrical cam 301 and the turntable 5 are driven to rotate in the opposite direction through the first guide groove 302, and then the convex rod 8 drives the moving block 7 to move in the opposite direction along the trajectory of the arc groove 6. When the convex block abuts against the top of the oblique groove of the first guide groove 302, the two gaskets 9 abut against each other and seal the pinhole, thereby preventing external air and dust from entering the bag body 1 through the pinhole, and at the same time the push plate 4 is reset, so as to facilitate the operator to use it next time.

[0024] like Figures 1 to 5 As shown, a convex rod 8 is fixedly connected to the side of the moving block 7 close to the turntable 5, and the convex rod 8 is movably engaged with the inside of the arc groove 6. When the push plate 4 moves toward the inside of the support block 2, it drives the cylindrical cam 301 to rotate through the first guide groove 302. When the cylindrical cam 301 rotates, it drives the turntable 5 to rotate and makes the two convex rods 8 move in opposite directions along the trajectory of the arc groove 6. Pinholes are provided in the middle of the surfaces of the push plate 4 and the turntable 5. The gasket 9 is made of rubber material. The two gaskets 9 are abutted in the initial state and seal the pinholes on the turntable 5. The inner wall of the bag body 1 is provided with a slide groove 10 that matches the moving block 7. The slide groove 10 is used for circumferential limitation of the moving block 7 when the turntable 5 rotates. The outer wall of the bag body 1 is provided with a circular groove that matches the pinhole on the turntable 5.

[0025] The above solution is adopted: the gasket 9 is made of rubber material. Its function is that since the rubber material has good elasticity, when the two gaskets 9 abut against each other, the two can fit tightly, thereby providing a reliable sealing effect, thereby effectively preventing liquid leakage and foreign matter from entering.

[0026] like Figure 1 , Figure 6 to Figure 7As shown, the sealing assembly 12 includes a moving rod 1201, and a round rod 1202 is arranged in a ring at one hundred and eighty degrees at one end of the moving rod 1201 close to the rotating rod 13. A ventilation groove 1203 is opened in the middle of the moving rod 1201, and the arc of the second guide groove 14 is one hundred and eighty degrees. The two round rods 1202 are respectively movably connected in the inside of the two second guide grooves 14. When the rotating rod 13 rotates, the round rod 1202 slides along the trajectory of the second guide groove 14 and drives the moving rod 1201 to move in the direction away from the rotating rod 13.

[0027] The above scheme is adopted: by rotating the rotating rod 13, the round rod 1202 slides along the track of the second guide groove 14, thereby driving the moving rod 1201 to move in the direction away from the rotating rod 13, and then limiting the moving rod 1201 through the straight groove 15, so that the moving rod 1201 drives the ventilation groove 1203 to coincide with the middle part of the pipe 11, thereby releasing the seal of the pipe 11, and facilitating the connection between the collection tube and the interior of the bag body 1.

[0028] like Figure 1 , Figure 6 to Figure 7 As shown, the pipe 11 is in a cross shape, and the diameter of the ventilation groove 1203 is the same as the inner diameter of the upper and lower ends of the pipe 11. The moving rod 1201 seals the middle part of the pipe 11 in the initial state. A block is provided at the end of the moving rod 1201 away from the round rod 1202. The inner wall of the pipe 11 is provided with a straight groove 15 that cooperates with the block. The straight groove 15 is used to limit the circumferential position of the moving rod 1201 when the rotating rod 13 rotates.

[0029] The above solution is adopted: the pipeline 11 is sealed by moving the rod 1201. Its function is to effectively prevent external dust, bacteria, impurities, etc. from entering the interior of the bag body 1 through the pipeline 11 when not in use, thereby maintaining a sterile environment inside the bag body 1, thereby ensuring the quality and safety of stem cells and preventing them from being contaminated and affecting their subsequent use and effects.

[0030] The working principle and use process of the present invention are as follows: when it is necessary to inject growth factors into the bag body 1, the operator places the needle tube on the push plate 4 so that the needle tip is inserted into the cylindrical cam 301 through the needle hole, and then presses the needle tube, thereby driving the push plate 4 to move toward the inside of the support block 2. While the push plate 4 moves, the convex block thereon slides along the track of the oblique groove of the first guide groove 302, thereby driving the cylindrical cam 301 and the turntable 5 thereon to rotate, and then the convex rod 8 drives the two moving blocks 7 to move in opposite directions along the track of the arc groove 6. When the convex block moves to the inside of the vertical groove, the moving block 7 drives the gasket 9 to release the seal of the needle hole on the turntable 5, and then continues to press the needle tube. When the convex block abuts against the inner wall of the vertical groove, the needle tip is inserted into the circular groove of the bag body 1 through the needle hole on the turntable 5, and then the growth factor is injected into the inside of the bag body 1. When it is necessary to insert the collection tube into the bag body 1, the operator rotates the rotating rod 13, so that the round rod 1202 slides along the trajectory of the second guide groove 14. While the round rod 1202 slides, it drives the moving rod 1201 to move away from the rotating rod 13. When the round rod 1202 abuts against the inner wall of the second guide groove 14, the ventilation groove 1203 coincides with the middle part of the pipe 11, thereby releasing the seal of the pipe 11, and then the collection tube is connected with the interior of the bag body 1 through the pipe 11.

[0031] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0032] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An integrated bag for separation and collection of stem cells, comprising a bag body (1), characterized in that: A support block (2) is fixedly connected to one side of the bag body (1), and a rotating drum assembly (3) is movably connected inside the support block (2); The rotating drum assembly (3) comprises a cylindrical cam (301), and a first guide groove (302) is formed on the inner wall of the cylindrical cam (301) at an angle of 180 degrees. The cylindrical cam (301) is movably connected to a push plate (4) inside, and a turntable (5) is fixedly connected to one end of the cylindrical cam (301) away from the push plate (4), and an arc groove (6) is provided on the surface of the turntable (5) in an annular manner at 180 degrees. The support block (2) is movably connected to a moving block (7) inside an annular manner at 180 degrees, and gaskets (9) are fixedly connected to opposite sides of the two moving blocks (7). Two pipes (11) are provided on the top of the bag body (1) in a mirrored manner, and a sealing assembly (12) is movably connected to the inside of the pipes (11), and a rotating rod (13) is movably connected to the opposite ends of the two pipes (11), and a second guide groove (14) is provided on the inner wall of the rotating rod (13) in an annular manner at 180 degrees.

2. The integrated bag for separation and collection of stem cells according to claim 1, characterized in that: The outer wall of the push plate (4) is provided with a protrusion at an angle of 180 degrees in an annular manner, the first guide groove (302) is composed of an inclined groove with an arc of 90 degrees and a vertical groove with an arc of zero degrees, and the protrusion is movably engaged with the top of the inclined groove in an initial state.

3. The integrated bag for stem cell separation and collection according to claim 1, characterized in that: The cylindrical cam (301) is movably connected to a support plate (303) inside, and the bottom of the push plate (4) is fixedly connected to a spring (304) fixedly connected to the support plate (303), and the spring (304) is used to press the push plate (4) and keep it moving toward the outside of the support block (2).

4. The integrated bag for separation and collection of stem cells according to claim 1, characterized in that: A convex rod (8) is fixedly connected to one side of the moving block (7) close to the rotating disk (5); the convex rod (8) is movably engaged in the interior of the arc groove (6); when the push plate (4) moves toward the interior of the support block (2), the cylindrical cam (301) is driven to rotate via the first guide groove (302); when the cylindrical cam (301) rotates, the rotating disk (5) is driven to rotate, and the two convex rods (8) are caused to move in opposite directions along the trajectory of the arc groove (6).

5. The integrated bag for separation and collection of stem cells according to claim 1, characterized in that: The push plate (4) and the rotating disk (5) are both provided with pinholes in the middle of their surfaces. The gasket (9) is made of a rubber material. The two gaskets (9) are in abutment with each other in an initial state and seal the pinholes on the rotating disk (5).

6. The integrated bag for stem cell separation and collection according to claim 1, characterized in that: The inner wall of the bag body (1) is provided with a slide groove (10) which cooperates with the moving block (7). The slide groove (10) is used to limit the circumferential position of the moving block (7) when the turntable (5) rotates. The outer wall of the bag body (1) is provided with a circular groove which cooperates with the pinhole on the turntable (5).

7. The integrated bag for separation and collection of stem cells according to claim 1, characterized in that: The sealing assembly (12) comprises a moving rod (1201), one end of the moving rod (1201) close to the rotating rod (13) is provided with a round rod (1202) in an annular shape at an angle of 180 degrees, and a ventilation groove (1203) is provided in the middle of the moving rod (1201).

8. The integrated bag for separation and collection of stem cells according to claim 7, characterized in that: The arc of the second guide groove (14) is one hundred and eighty degrees, and the two round rods (1202) are respectively movably engaged in the interior of the two second guide grooves (14). When the rotating rod (13) rotates, the round rod (1202) slides along the track of the second guide groove (14) and drives the moving rod (1201) to move in a direction away from the rotating rod (13).

9. The integrated bag for separation and collection of stem cells according to claim 7, characterized in that: The pipe (11) is in a cross shape, the diameter of the ventilation groove (1203) is the same as the inner diameter of the upper and lower ends of the pipe (11), and the moving rod (1201) seals the middle part of the pipe (11) in the initial state.

10. The integrated bag for separation and collection of stem cells according to claim 7, characterized in that: A clamping block is provided at one end of the moving rod (1201) away from the round rod (1202), and a straight slot (15) matching the clamping block is provided on the inner wall of the pipe (11). The straight slot (15) is used to limit the circumferential position of the moving rod (1201) when the rotating rod (13) rotates.

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