Compound additive formula for improving meat quality of sheep

Abstract

The invention belongs to the technical field of animal feed, and discloses a compound additive formula for improving the meat quality of sheep, which is characterized in that selenomethionine, curcumin, angelica sinensis and pericarpium citri reticulatae are added into the basic ration of the sheep. The compound additive formula disclosed by the invention is beneficial to improving the tenderness of sheep meat and improving the meat color, so that the mutton quality is improved.

Description

Technical Field

[0001] The invention belongs to the technical field of animal feed, and in particular relates to a composite additive formula for improving sheep meat quality. Background Art

[0002] As one of the important meat sheep breeds in China, the meat quality characteristics of sheep directly affect the economic benefits and consumer acceptance. Research on improving the quality of sheep meat is an important topic with great economic value. Among the many factors affecting meat quality, the optimization of nutritional components and the rational application of additives are particularly important. Based on this, we propose a composite additive to improve the quality of sheep meat. Summary of the invention

[0003] The invention aims to provide a composite additive formula for improving the quality of sheep meat, comprising adding selenomethionine, curcumin, angelica sinensis and tangerine peel into the basic diet of sheep.

[0004] Preferably, the weight proportions of the composite additive in each kilogram of basic diet are as follows: selenomethionine is added in an amount of 0.6 mg to 0.9 mg; curcumin is added in an amount of 200 mg to 240 mg; angelica is added in an amount of 6 g to 9 g; and tangerine peel is added in an amount of 3 g to 5 g.

[0005] Preferably, the weight proportions of the composite additive in each kilogram of basic diet are as follows: selenomethionine is added in an amount of 0.6 mg; curcumin is added in an amount of 200 mg; angelica is added in an amount of 6 g; and tangerine peel is added in an amount of 3 g.

[0006] Preferably, vitamins and trace elements are added to the basic diet, and the weight proportions of vitamins and trace elements added per kilogram of the basic diet are: 153-155 mg feed grade MgS04·7H2O (Mg>9.5%), 30-32 mg feed grade CuS04·5H2O (Cu>25%), 45.5-46.5 mg feed grade FeS04·H2O (Fe>29%), 56.0-56.2 mg feed grade CuS04·5H2O (Cu>25 ... g feed grade ZnO (Zn>70%), 61~61.5mg feed grade MnH2O (Mn>30%), 1~2mg feed grade NaSeO3 (Se>1%), 0.25~0.27mg feed grade KI (I>1%), 0.26~0.28mg feed grade CoC12·H2O (Co>1%), 1.3~1.5g stone powder (Ca>34%), 0.1g~0.2g zeolite powder; Vitamin A 1000IU / kg~1200IU / kg, Vitamin D3 150IU / kg~200IU / kg, Vitamin 30IU / kg~40IU / kg.

[0007] The present invention has the following beneficial effects:

[0008] The present invention, on the basis of meeting the requirements of trace elements and vitamins in the "Standards for Sheep Breeding" (NY / T 816-2004), specially adds selenomethionine, curcumin, angelica and tangerine peel. Selenomethionine is an organic selenium additive, which can supplement the selenium element required by animals, improve the body's antioxidant capacity, and improve meat quality; curcumin may improve meat quality through antioxidant and anti-inflammatory effects; angelica is sweet and zinc, warm in nature, belongs to the liver meridian, heart meridian, and spleen meridian, has the effect of nourishing blood and regulating menstruation, and can be used together with tangerine peel to enhance the effect of nourishing blood and regulating menstruation; tangerine peel tastes bitter and pungent, warm in nature, belongs to the spleen meridian and lung meridian, has the effect of drying dampness and resolving phlegm, regulating qi and strengthening spleen, and can enhance the effect of regulating qi and strengthening spleen by combining with angelica. Traditional Chinese medicine believes that "qi is the leader of blood, and blood is the mother of qi", tangerine peel regulates qi, angelica nourishes blood, and the two are used in conjunction with each other, and together have the effects of promoting blood circulation and nourishing blood, strengthening spleen and drying dampness, and the sheep are strong when qi and blood are full and the spleen and stomach are healthy. The composite additive formula of the present invention is helpful to improve the tenderness of sheep meat, improve the meat color, and thus improve the quality of sheep meat. BRIEF DESCRIPTION OF THE DRAWINGS

[0009] Figure 1 3 is a comparison diagram of the shear force of each group of sheep muscles in the embodiment of the present invention.

[0010] Figure 2 3 is a comparison diagram of the redness of each group of sheep muscles in the embodiment of the present invention.

[0011] Figure 3 3 is a comparison diagram of the yellowness of each group of sheep muscles in the embodiments of the present invention. DETAILED DESCRIPTION

[0012] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0013] A composite additive formula for improving the quality of sheep meat comprises adding selenomethionine, curcumin, angelica sinensis and tangerine peel to the basic diet of sheep. The composite additive formula is used for improving the quality of sheep meat.

[0014] Preferably, the weight proportions of the composite additives in each kilogram of basic diet are as follows: selenomethionine is added in an amount of 0.6 mg to 0.9 mg; curcumin is added in an amount of 200 mg to 240 mg; angelica is added in an amount of 6 g to 9 g; and tangerine peel is added in an amount of 3 g to 5 g.

[0015] Preferably, the weight proportions of the composite additives in each kilogram of basic diet are as follows: selenomethionine is added in an amount of 0.6 mg; curcumin is added in an amount of 200 mg; angelica is added in an amount of 6 g; and tangerine peel is added in an amount of 3 g.

[0016] As a preferred method, based on the trace element and vitamin requirements of the "Meat Sheep Feeding Standard" (NY / T 816-2004), the weight portions of vitamins and trace elements added to each kilogram of basic diet are: 153-155 mg feed grade MgS04·7H2O (Mg>9.5%), 30-32 mg feed grade CuS04·5H2O (Cu>25%), 45.5-46.5 mg feed grade FeS04·H2O (Fe>29%), 56.0-56.2 mg feed grade CuS04·5H2O (Cu>25%), 10.5-11.5 mg feed grade CuS04·5H2O (Cu>25%), 15.0-16.5 mg feed grade CuS04·5H2O (Cu>25%), 20.0-20.5 mg feed grade CuS04·5H2O (Cu>25%), 30.0-3 ... Feed grade ZnO (Zn>70%), 61-61.5mg feed grade MnH2O (Mn>30%), 1-2mg feed grade NaSeO3 (Se>1%), 0.25-0.27mg feed grade KI (I>1%), 0.26-0.28mg feed grade CoC12·H2O (Co>1%), 1.3-1.5g stone powder (Ca>34%), 0.1g-0.2g zeolite powder; Vitamin A 1000IU / kg-1200IU / kg, Vitamin D3 150IU / kg-200IU / kg, Vitamin 30IU / kg-40IU / kg.

[0017] The following is a specific description through examples.

[0018] 1. Feed preparation

[0019] (1) Prepare main feed raw materials and carrier materials such as corn and bran;

[0020] (2) Testing the conventional nutrients and harmful substances of the main raw materials;

[0021] (3) Crush the main raw material and calculate the combination of the main raw material and other feed raw materials;

[0022] (4) using various trace element raw materials and inorganic carriers to produce a composite trace element premix for standby use;

[0023] (5) using various vitamin raw materials, antioxidants and organic carriers to produce a multivitamin premix for later use;

[0024] (6) Use amino acids, fatty acids, metabolic regulators and carriers to produce other additive premixes for future use;

[0025] (7) Selenomethionine (bag powder): weigh the required amount accurately according to the designed dosage, label it clearly and keep it ready for use;

[0026] (8) Curcumin (bag powder): accurately weigh the required amount according to the experimental design, label it clearly, and set aside;

[0027] (9) Crush the angelica and dried tangerine peel, pass through an 80-mesh sieve, and crush the part that does not pass through the sieve again until all of it passes through the 80-mesh sieve. Label it and set aside.

[0028] (10) According to the formula of composite premix for mutton, the above-mentioned (1) to (9) and other materials are respectively measured and mixed to produce a composite additive premix;

[0029] (11) According to the production formula, each main raw material and the above-mentioned (10) (composite additive premix) are weighed separately, added into a mixer and mixed for 2 to 3 minutes to make the uniformity (CV value) reach 7%;

[0030] (12) The powdery substance prepared in (11) is granulated by a granulating device and then packaged to obtain a complete mixed feed pellet feed product for mutton sheep, which is then packaged.

[0031] Feed storage and use methods:

[0032] Storage: The finished feed should be stored in a cool and dry place with a shelf life of 90 days.

[0033] Usage: Feed 0.5-1.0 kg per sheep per day and allow the sheep to eat freely.

[0034] 2. Verification Test

[0035] The basal diet was prepared with reference to the "Standards for Sheep Feeding" (NY / T 816-2004). The experiment included a 14-day pre-feeding period and a 63-day test period. The experimental animals were fed the basal diet during the pre-feeding period, and no additional selenium and curcumin were added to the basal diet. The control group was fed the basal diet during the test period. The raw material composition and nutritional level of the basal diet are shown in Table 1.

[0036] Table 1 Basic diet raw material composition and nutritional level (dry matter basis)

[0037]

[0038]

[0039] 1 Note: The premix provides per kilogram of feed: iron Fe 15 mg, manganese Mn 20 mg, zinc Zn 25 mg, copper Cu 8 mg, iodine I 0.2 mg, cobalt Co 0.15 mg, vitamin A VA 1000 IU, vitamin D VD 150 IU, vitamin E VE 30 IU.

[0040] Selenomethionine (SeMet), curcumin, angelica, tangerine peel, trace elements and vitamins are combined to make a premix and added to the feed.

[0041] Forty-four 4-month-old male lambs (32.86±2.86 kg) were randomly divided into 11 groups, including

[0042] Control group (fed with basal diet);

[0043] Group A: 0.45 mg / kg selenomethionine (SeMet);

[0044] Group B: 0.6 mg / kg selenomethionine (SeMet);

[0045] Group C: 0.9 mg / kg selenomethionine (SeMet);

[0046] Group D: 6g / kg Chinese Angelica sinensis, 3g / kg dried orange peel;

[0047] Group E: 0.45 mg / kg selenomethionine (SeMet), 200 mg / kg curcumin group;

[0048] Group F: 0.6 mg / kg selenomethionine (SeMet), 200 mg / kg curcumin group;

[0049] Group G: 0.9 mg / kg selenomethionine (SeMet), 200 mg / kg curcumin group;

[0050] Group H: 0.6 mg / kg selenomethionine (SeMet), 220 mg / kg curcumin group;

[0051] Group I: 0.6 mg / kg selenomethionine (SeMet), 240 mg / kg curcumin group;

[0052] Group J: 0.6 mg / kg selenomethionine (SeMet), 200 mg / kg curcumin, 6 g / kg angelica, 3 g / kg tangerine peel. Each group had 4 replicates, with 1 sheep in each replicate.

[0053] Before the experiment officially started, all the experimental sheep were dewormed and vaccinated, and the sheep house and experimental site were thoroughly cleaned and disinfected. The experimental sheep were fed in a single pen, and were fed twice a day at 08:00 and 18:00, and had free access to food and water.

[0054] After the 63-day fattening experiment, water and feed were cut off 1 day before the end of the experiment, and slaughter and sampling were carried out. Four sheep were randomly selected from each group for slaughter. After the experimental sheep were slaughtered and the carcass weight was weighed, the longissimus dorsi muscle sample on the left side of the carcass was taken to measure the meat quality. The fat and fascia of the longissimus dorsi muscle were removed, and the meat color was measured for 45 minutes. After completion, the muscle samples were stored in an acid drainage box for 24 hours. The acid drainage box was set at 4°C and 90% humidity. The meat color was measured using a handheld colorimeter (CR-400 / 410). The shear force was measured using a muscle tenderness shear force meter (NDY-100-200-A).

[0055] Excel 2021 was used for experimental data statistics, and SPSS 26.0 was used for data analysis. The differences between groups were compared using the one-way ANOVA test procedure for mean values, and Duncan's method was used for post hoc multiple comparisons. The difference was significant when P < 0.05. As shown in Table 2.

[0056] Table 2 Effects of different treatment groups on muscle quality of fattening sheep

[0057] Group\Project Shear force(N) Redness a* Yellowness b* Comparison 54.86 16.93 10.34 A 52.13 17.21 10.59 B 52.12 17.35 10.88 C 52.05 17.54 10.74 D 52.85 17.35 10.39 E 51.68 20.11 12.35 F 51.84 20.15 12.42 G 52.05 21.32 12.57 H 52.08 21.52 12.68 I 51.84 21.32 12.65 J <![CDATA[51.42 a ]]> <![CDATA[22.10 b ]]> <![CDATA[13.10 c ]]> P-value ＜0.000 ＜0.000 ＜0.000

[0058] Note: The same letters in the same row of data indicate no significant difference (P>0.05), and different letters indicate significant difference (P<0.05)

[0059] Compared with the control group fed with the basic diet, the muscle shear force of sheep in the group A supplemented with 0.45mg / kg selenomethionine (SeMet) was reduced by 4.98%; the muscle shear force of sheep in the group B supplemented with 0.60mg / kg selenomethionine (SeMet) was reduced by 4.99%; the muscle shear force of sheep in the group C supplemented with 0.90mg / kg selenomethionine (SeMet) was reduced by 5.12%; the muscle shear force of sheep in the group D supplemented with 6g angelica and 3g tangerine peel was reduced by 3.66%; the muscle shear force of sheep in the group E supplemented with 0.45mg / kg selenomethionine (SeMet) and 200mg / kg curcumin was reduced by 5.80%; the muscle shear force of sheep in the group F supplemented with 0.6mg / kg selenomethionine (SeMet) and 200mg / kg curcumin added group sheep muscle shear force decreased by 5.50%; G group 0.9mg / kg selenomethionine (SeMet), 200mg / kg curcumin added group sheep muscle shear force decreased by 5.12%; H group 0.6mg / kg selenomethionine (SeMet), 220mg / kg curcumin added group sheep muscle shear force decreased by 5.07%; I group 0.6mg / kg selenomethionine (SeMet), 240mg / kg curcumin added group sheep muscle shear force decreased by 5.50%; J group 0.6mg / kg selenomethionine (SeMet), 200mg / kg curcumin, 6g / kg angelica, 3g / kg tangerine peel added group sheep muscle shear force decreased by 6.27%.

[0060] The groups supplemented with 0.6mg / kg selenomethionine (SeMet), 200mg / kg curcumin, 6g / kg angelica, and 3g / kg tangerine peel significantly reduced the shear force of fattening sheep muscle, with a reduction of 6.27% ( Figure 1 ). The meat color test showed that compared with the control group, groups A, B, and C had no significant effect on the redness of sheep muscle. However, groups D, E, F, G, H, and I increased the redness of sheep meat to varying degrees; group J significantly increased the redness of sheep muscle by 18.8% ( Figure 2 ). Groups A, B, C, D, E, F, G, H, I and J were all able to increase the yellowness of sheep muscle, with the increase in Group J reaching 19.1% ( Figure 3 ).

[0061] In summary, the group supplemented with 0.6mg / kg selenomethionine (SeMet), 200mg / kg curcumin, 6g / kg angelica, and 3g / kg tangerine peel reduced the shear force of fattening sheep muscle by 6.27%, increased the redness of sheep muscle by 18.8%, and increased the yellowness of mutton by 19.1%. The shear force was reduced while the redness and yellowness were increased, which improved the quality of mutton.

[0062] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A composite additive formula for improving the quality of sheep meat, characterized in that: It involves adding selenomethionine, curcumin, angelica sinensis and tangerine peel to the basal diet of sheep.

2. The composite additive formula for improving the quality of sheep meat according to claim 1, characterized in that: The weight proportions of the composite additive in each kilogram of basic diet are as follows: the addition amount of selenomethionine is 0.6mg-0.9mg; the addition amount of curcumin is 200mg-240mg; the addition amount of angelica is 6g-9g; and the addition amount of tangerine peel is 3g-5g.

3. The composite additive formula for improving the quality of sheep meat according to claim 2, characterized in that: The weight proportions of the composite additive in each kilogram of basic diet are as follows: 0.6 mg of selenomethionine, 200 mg of curcumin, 6 g of angelica sinensis, and 3 g of tangerine peel.

4. The composite additive formula for improving the quality of sheep meat according to claim 1, characterized in that: Vitamins and trace elements are added to the basic diet, and the weight proportions of the vitamins and trace elements added per kilogram of the basic diet are: 153-155 mg feed grade MgS04·7H2O (Mg>9.5%), 30-32 mg feed grade CuS04·5H2O (Cu>25%), 45.5-46.5 mg feed grade FeS04·H2O (Fe>29%), 56.0-56.2 mg feed grade ZnO (Zn>70%), 61-61.5mg feed grade MnH2O (Mn>30%), 1-2mg feed grade NaSeO3 (Se>1%), 0.25-0.27mg feed grade KI (I>1%), 0.26-0.28mg feed grade CoC12·H2O (Co>1%), 1.3-1.5g stone powder (Ca>34%), 0.1g-0.2g zeolite powder; Vitamin A 1000IU / kg-1200IU / kg, Vitamin D3 150IU / kg-200IU / kg, Vitamin 30IU / kg-40IU / kg.

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