Anti-aging composition for fading structural dark circles and eye fine wrinkles and application of anti-aging composition

Abstract

The invention discloses an anti-aging composition for fading structural dark circles and eye fine lines and application of the anti-aging composition, and relates to the technical field of cosmetics. The invention provides an anti-aging composition. The anti-aging composition is prepared from the following components in parts by weight: 0.01 to 0.2 part of an artichoke extract, 0.01 to 1 part of a coleus forskohlii extract, 0.01 to 2 parts of mu-conopeptide and 0.01 to 3 parts of a lactobacillus crispatus fermentation product. The invention relates to an extract of Rhododendron simsii, which is characterized by comprising ruscogenin C27H42O4 and neoruscogenin C27H40O4. According to the anti-aging composition provided by the invention, when the four components, namely, the rhododendron forskohlii extract, the coleus forskohlii extract, the mu-conopeptide and the lactobacillus crispatus fermentation product, are synergistic, the effects of enhancing the skin compactness and elasticity, effectively fading structural dark circles and smoothing fine lines of eyes can be instantly reflected and continuously kept.

Description

Technical Field

[0001] The invention relates to the technical field of cosmetics, in particular to an anti-aging composition for reducing structural dark circles and eye fine lines and application thereof. Background Art

[0002] With the accelerated pace of modern life, staying up late, using electronic products for a long time and overusing eyes have become common phenomena. These bad living habits cause the area around the eyes to become the first part of the face to age and the part with the highest degree of aging, which is manifested as structural dark circles, eye bags and fine lines around the eyes. Sensitive skin, due to its weak barrier function and susceptibility to irritation, is often more susceptible to inflammatory aging, which aggravates these problems. Therefore, it is particularly important to develop eye protection products for sensitive skin to meet its dual needs for mildness and effectiveness.

[0003] Among the existing technologies, medical beauty technologies such as laser treatment, liposuction surgery and injectable fillers have good effects on structural dark circles and eye bags, but surgery is risky, accompanied by side effects and is expensive; common eye cream active ingredients such as retinol and caffeine have anti-aging and eye puffiness improvement effects respectively, but the effects of these ingredients are often limited to the surface, and it is difficult to deeply improve the problems of structural dark circles and fine lines, and some ingredients may irritate sensitive skin and cause allergic reactions.

[0004] Therefore, an anti-aging composition that enhances skin firmness and elasticity, effectively reduces structural dark circles and smoothes fine lines around the eyes, and enhances the overall youthfulness of the eye skin has broad application prospects. Summary of the invention

[0005] Based on this, the purpose of the present invention is to overcome the shortcomings of the above-mentioned prior art and provide an anti-aging composition and its preparation method and application that enhances skin firmness and elasticity, effectively reduces structural dark circles and smoothes eye fine lines, and enhances the overall youthfulness of eye skin.

[0006] To achieve the above object, the technical scheme adopted by the present invention is: an anti-aging composition, comprising the following components in parts by weight: 0.01-0.2 parts of Ruscus aconitifolia extract, 0.01-1 parts of Coleus forskohlii extract, 0.01-2 parts of μ-conopeptide, and 0.01-3 parts of Lactobacillus crispatus fermentation product; the Ruscus aconitifolia extract includes ruscosaponin C 27 H 42 O4 and neoruscosapogenin C 27 H 40 O4.

[0007] The extract of Ruscus aconite has significant anti-inflammatory, antibacterial and capillary permeability reducing effects, can reduce capillary fluid extravasation, and thus alleviate edema; the Ruscus aconite extract selected by the present invention includes at least one of Ruscus aconite (root) extract, Ruscus aconite (rhizome) extract, and Ruscus aconite (leaf) extract; the main active ingredient of the Coleus forskohlii extract is forskolin, which can stimulate the activity of adenylate cyclase and increase the level of cellular cyclic adenosine monophosphate cAMP, thereby promoting the metabolism of local fat and improving the appearance of eye bags that have already formed in the lower eyelid; μ-conopeptide is a bioactive peptide extracted from cone snails (Ipomoea), which can block muscle nerve conduction, thereby enhancing skin firmness and reducing wrinkles; the Lactobacillus crispatus is a specific bacterium living in the crow's feet area, disappears on aging skin over time, and its fermentation product promotes skin hydration, reduces skin dryness and relaxation, can effectively enhance skin elasticity, and has a significant effect on improving fine lines around the eyes.

[0008] The inventors of the present application found in actual experiments that when the four components of Butcher's Pear extract, Coleus Forskohlii extract, μ-conopeptide, and Lactobacillus crispatus fermentation product are used synergistically, they can synergistically enhance skin firmness and elasticity, effectively fade structural dark circles and smooth fine lines around the eyes, and enhance the overall youthfulness of the eye skin.

[0009] Preferably, the anti-aging composition comprises the following components in parts by weight: 0.02-0.1 parts of Ruscus aconitifolia extract, 0.05-0.5 parts of Coleus forskohlii extract, 0.05-0.5 parts of μ-conopeptide, and 0.1-2 parts of Lactobacillus crispatus fermentation product.

[0010] Preferably, the following components are included in parts by weight: 0.05-0.07 parts of Ruscus aconitifolia extract, 0.2-0.3 parts of Coleus forskohlii extract, 0.1-0.2 parts of μ-conopeptide, and 0.5-1 parts of Lactobacillus crispatus fermentation product.

[0011] The inventors of the present application found in actual experiments that the weight of the components in the anti-aging composition will affect the performance of the product. When the weight of the components is further selected to be within the above range, the obtained product has a more significant effect of enhancing skin firmness and elasticity, fading structural dark circles and smoothing fine lines around the eyes.

[0012] Preferably, in the Ruscus aconitum extract, the weight ratio of ruscosapogenin to neoruscosapogenin is 1:(0.2-2.5). Further preferably, in the Ruscus aconitum extract, the weight ratio of ruscosapogenin to neoruscosapogenin is 1:(0.7-1.4); more preferably, in the Ruscus aconitum extract, the weight ratio of ruscosapogenin to neoruscosapogenin is 1:(0.9-1.2).

[0013] The inventors of the present application found in actual experiments that the weight ratio of ruscosaponin and neoruscosaponin in the false leaf tree extract will further affect the performance of the prepared anti-aging composition. When the weight ratio of ruscosaponin and neoruscosaponin is within the above range, the obtained product has a more significant effect of enhancing skin firmness and elasticity, fading structural dark circles and smoothing fine lines around the eyes.

[0014] Preferably, the preparation method of the Butcher's Broom extract is as follows:

[0015] (1) grinding and crushing the leaves and / or rhizomes of Ruscus rubra and / or roots of Ruscus rubra, sieving to obtain a coarse powder, acid hydrolyzing, reflux extraction, and filtering to obtain a filtrate;

[0016] (2) extracting the filtrate and centrifuging it, collecting the organic phase, washing it with alkali and drying it to powder to obtain the Ruscus aculeatus extract.

[0017] Preferably, in step (1), the weight ratio of the Ruscus aconitifolia leaves to the Ruscus aconitifolia rhizomes is 1:(0.5-5); and / or the weight ratio of the Ruscus aconitifolia leaves to the Ruscus aconitifolia roots is 1:(2-7).

[0018] Preferably, in the step (1), after grinding and pulverizing, the product is sieved through an 80-200 mesh sieve, the solvent for acidic hydrolysis is at least one of an aqueous solution of hydrochloric acid / sulfuric acid and an alcohol solution of hydrochloric acid / sulfuric acid, the acid concentration is 0.2-2.0 mol / L, and the mass ratio of the coarse powder to the solvent is 1 g:(40-60) mL; the temperature for reflux extraction is 70-90° C., and the time for reflux extraction is 2-4 h; in the step (2), the extraction solvent is at least one of n-hexane and dichloromethane, the volume ratio of the extraction solvent to the filtrate is (1-2):1, the centrifugal speed is 1500-3000 rpm, and the centrifugal time is 1-5 min; the alkali for the alkali washing is sodium hydroxide, and the weight ratio of the alkali solution for the alkali washing to the organic phase is 1:(3-6); and the drying is carried out in a nitrogen environment at 50-65° C.

[0019] In addition, the present invention provides the use of the anti-aging composition in preparing skin products.

[0020] Optionally, the skin products include at least one of lotion, emulsion, cream, facial mask, essence, spray and eye cream.

[0021] Furthermore, the present invention provides a skin product, which comprises the following components in percentage by mass: 1-20% of an anti-aging composition, 5-40% of a cosmetic base, and the remainder being water; the cosmetic base comprises at least one of a thickener, a moisturizer, an emulsifier, a preservative, and a pH adjuster.

[0022] Optionally, the present invention provides an eye cream comprising the following components in percentage by weight: 1-20% anti-aging composition, 0.05-0.5% thickener, 0.5-2% moisturizer, 5-20% emulsifier, 0.5-3% preservative, 0.01-0.3% pH adjuster, and the balance deionized water.

[0023] Optionally, the eye cream comprises at least one of the following (a)-(e):

[0024] (a) the thickener comprises at least one of xanthan gum, carbomer, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, ammonium acryloyldimethyl taurate / VP copolymer, sclerotium gum, and cetyl alcohol;

[0025] (b) the moisturizing agent comprises at least one of allantoin, sodium polyacrylate, hydrogenated lecithin, betaine, β-glucan, trehalose, caprylyl glycol, dipropylene glycol, sodium hyaluronate, 1,2-butylene glycol, glycerol, budding septin polysaccharide, and ceramide;

[0026] (c) the pH adjusting agent comprises at least one of arginine, tromethamine, and EDTA-disodium;

[0027] (d) the emulsifier comprises at least one of caprylic capric triglyceride, hexyldecanol, C14-22 alcohol, C12-20 alkyl glucoside, cetearyl glucoside, isononyl isononanoate, pentaerythritol tetrastearate, polydimethylsiloxane, stearyl alcohol, hydroxystearic acid, polymethylsilsesquioxane, pentaerythritol distearate, and sucrose stearate;

[0028] (e) The preservative includes at least one of 1,3-propylene glycol, 1,2-propylene glycol, 1,2-hexanediol, p-hydroxyacetophenone, and acrylates / C10-30 alkyl acrylate cross-linked polymer.

[0029] Compared with the prior art, the beneficial effects of the present invention are as follows: (1) The inventors of the present invention have found in actual experiments that when the four components of the Ruscus aconite extract, the Coleus forskohlii extract, the μ-conopeptide, and the fermentation product of Lactobacillus crispatus work together, the skin firmness and elasticity can be synergistically enhanced, the structural dark circles can be effectively lightened, the fine lines around the eyes can be smoothed, and the overall youthfulness of the skin around the eyes can be improved. (2) Ruscosapogenin and neoruscosapogenin in the Ruscus aconite extract of the present application can reduce capillary permeability and edema, and instantly improve the volume of dark circles. The μ-conopeptide can inhibit muscle nerve impulses, enhance skin firmness, and smooth fine lines around the eyes, and the effect is immediate. When the four components of the Ruscus aconite extract, the Coleus forskohlii extract, the μ-conopeptide, and the fermentation product of Lactobacillus crispatus work together, the skin firmness and elasticity can be enhanced, and the effect of effectively lightening structural dark circles and smoothing fine lines around the eyes can be immediately reflected and maintained continuously. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 These are the dark circle improvement pictures of volunteers before using eye cream, 30 minutes after using eye cream, and 28 days after using eye cream for the blank application example, application example 1, and application example 3; among them, (a) is the picture of dark circles before use, (b) is the picture of dark circles after using eye cream for 30 minutes, and (c) is the picture of dark circles after using eye cream for 28 days. DETAILED DESCRIPTION

[0031] In order to better illustrate the purpose, technical scheme and advantages of the present invention, the present invention will be further described below in conjunction with the accompanying drawings and specific embodiments. Its purpose is to understand the content of the present invention in detail, rather than to limit the present invention. All other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention. Unless otherwise specified, the experimental reagents and instruments designed for the implementation and comparative examples of the present invention are all commonly used ordinary reagents and instruments, which can be obtained from commercial channels. In the implementation and comparative examples, the experimental methods used are conventional methods unless otherwise specified; and unless otherwise specified, the raw materials used in parallel experiments are the same batch of raw materials.

[0032] The raw materials used in the embodiments of the present invention are now further described, but are not limited to the following raw materials:

[0033] Coleus forskohlii extract: purchased from Shaanxi Ruilin Panier Biotechnology Co., Ltd.;

[0034] μ-conopeptide: purchased from Shenzhen Weiqi Biotechnology Co., Ltd.;

[0035] Lactobacillus crispatus fermentation product: purchased from BASF, Germany;

[0036] Commercially available Ruscus aconitifolia extract: purchased from NORMACTIVE, Türkiye;

[0037] Aesculus hippocastanum extract: purchased from Xi'an Jinheng Chemical Co., Ltd.;

[0038] Palmitoyl tetrapeptide-7: purchased from Guangzhou Baiyu Biotechnology Co., Ltd.;

[0039] The filtrate of the fermentation product of bifid yeast was purchased from Guangzhou Baiyu Biotechnology Co., Ltd.

[0040] Butcher's Broom Extract-1

[0041] The preparation method of the Butcher's Broom extract-1 comprises the following steps:

[0042] (1) The leaves of the Ascaris chinensis are ground and crushed, and then passed through an 80-mesh sieve. After acid hydrolysis, the leaves are refluxed and extracted at a temperature of 80° C. for 3 h, and then filtered to obtain a filtrate. The solvent for the acid hydrolysis is a 70% isopropanol solution containing 1 mol / L sulfuric acid, and the mass ratio of the coarse powder to the solvent is 1 g:50 mL.

[0043] (2) extracting the filtrate and then centrifuging it, collecting the organic phase, washing it with sodium hydroxide aqueous solution and drying it to powder to obtain the Ruscus aconitifolia extract; the extraction solvent is n-hexane, the volume ratio of the extraction solvent to the filtrate is 1:1, the centrifugal speed is 2000 rpm, and the centrifugal time is 3 min; the weight ratio of the 1 mol / L sodium hydroxide aqueous solution to the organic phase is 1:5; and the drying is carried out in a nitrogen environment at 60°C.

[0044] Ruscus aconite extract-2~Ruscus aconite extract-9

[0045] The preparation methods of the Ruscus aconitifolia extract-2 to Ruscus aconitifolia extract-9 are compared with the preparation method of the Ruscus aconitifolia extract-1, except that the proportion of the raw materials in step (1) is different, as shown in Table 1 below, and the remaining components, weight parts and preparation methods are exactly the same.

[0046] Butcher's Broom Extract-10

[0047] The preparation method of the Butcher's Broom Extract-10 comprises the following steps:

[0048] (1) The leaves of the Ruscus scabra were ground and crushed, and then passed through a 150-mesh sieve. After acid hydrolysis, they were refluxed and extracted. The reflux extraction temperature was 70° C., and the reflux extraction time was 4 h. After filtering, a filtrate was obtained. The solvent for the acid hydrolysis was an aqueous solution containing 1 mol / L hydrochloric acid, and the mass ratio of the crude powder to the solvent was 1 g: 60 mL.

[0049] (2) extracting the filtrate and then centrifuging it, collecting the organic phase, washing it with sodium hydroxide aqueous solution and drying it to powder to obtain the Ruscus aconitifolia extract; the extraction solvent is dichloromethane, the volume ratio of the extraction solvent to the filtrate is 2:1, the centrifugal speed is 3000 rpm, and the centrifugal time is 2 min; the weight ratio of the 0.5 mol / L sodium hydroxide aqueous solution to the organic phase is 1:3; and the drying is carried out in a nitrogen environment at 50°C.

[0050] In the Ruscus aconitifolia extract, the relationship between the raw materials and the weight ratio of ruscosapogenin and neoruscosapogenin in the Ruscus aconitifolia extract are shown in Table 1.

[0051] The test method of ruscosaponin and new ruscosaponin in the extract of Ruscoleaf is as follows: 1 mg of the extract of Ruscoleaf is accurately weighed, dissolved in 10 mL of methanol, and after passing through a 0.22 μm filter membrane, the content of ruscosaponin and new ruscosaponin in the extract is determined by high performance liquid chromatography and the ratio is calculated. The chromatographic column is a C18 column (100×3.0 mm, 3.5 μm), the mobile phase is acetonitrile (70%): 0.1% formic acid water (30%), the flow rate is 1 mL / min, the column temperature is 45°C, and the sample load is 10 μL. The ratio of ruscosaponin and new ruscosaponin is calculated according to the following formula: ratio=ruscosaponin peak area / new ruscosaponin peak area.

[0052] Table 1

[0053] Case Raw material (Ruscus aconite) Ruscosaponin: Neoruscosaponin Butcher's Broom Extract-1 blade 1:0.2 Butcher's Broom Extract-2 Leaf: rhizome = 1:0.5 1:0.7 Butcher's Broom Extract-3 Leaves: rhizome = 1:1 1:0.9 Butcher's Broom Extract-4 Leaves: rhizome = 1:2 1:1.2 Butcher's Broom Extract-5 Leaves: rhizome = 1:5 1:1.4 Butcher's Broom Extract-6 rhizome 1:1.6 Butcher's Broom Extract-7 root 1:2.3 Butcher's Broom Extract-8 Leaf: root = 1:1 1：0.5 Butcher's Broom Extract-9 Root: rhizome = 1:1 1：1.7 Butcher's Broom Extract-10 blade 1:0.2 Commercially available Ruscus rubra extract - 1：1.8

[0054] Examples and Comparative Examples

[0055] The present invention provides an anti-aging composition, the components and weight portions of the anti-aging composition are selected as shown in Table 2-3, and the preparation method of the anti-aging composition comprises the following steps: dissolving a Ruscus aconitifolia extract and a Coleus forskohlii extract in 6 weight portions of hexyldecanol, dissolving μ-conopeptide and a fermentation product of Lactobacillus crispatus in 4 weight portions of water, and then uniformly mixing the two to obtain the anti-aging composition.

[0056] Table 2

[0057]

[0058]

[0059] Table 3

[0060]

[0061] Application Examples

[0062] The application examples of the present invention provide an eye cream, the components (mass percentage) of the eye cream are as shown in Table 4; wherein the compositions in application examples 1-16 respectively use the anti-aging compositions prepared in embodiments 1-16; the anti-aging compositions in comparative application examples 1-6 respectively use the anti-aging compositions prepared in comparative examples 1-6; for example, the anti-aging composition used in application example 1 is the anti-aging composition in embodiment 1, the anti-aging composition used in application example 5 is the anti-aging composition in embodiment 5, the anti-aging composition used in application example 15 is the anti-aging composition in embodiment 15, and the same applies to the others; the anti-aging composition used in application examples 17-18 is the anti-aging composition prepared in embodiment 3.

[0063] Table 4

[0064]

[0065] The preparation method of the eye cream provided in Application Example 1 comprises the following steps:

[0066] (1) Mix the humectant and thickener with water, heat to 85±2° C., homogenize at 1200 rpm for 4 min, and keep warm for later use to obtain prefabricated component A;

[0067] (2) Mix the emulsifier, heat to 85±2° C., homogenize at 1200 rpm for 4 min, and keep warm for later use to obtain prefabricated component B;

[0068] (3) Mix the preservatives and heat to 60±2°C to melt to obtain a prefabricated C component;

[0069] (4) Heat the preformed component A to 80±2°C, add the preformed component B at a speed of 250 rpm, stir and mix, then cool to 60±2°C, add the preformed component C at a speed of 250 rpm, stir and mix, then cool to 40°C, add the anti-aging composition and continue stirring for 8 minutes, finally add the remaining pH adjuster to adjust the pH to 6.0, then stop stirring, discharge, and obtain the eye cream.

[0070] The preparation methods of the remaining application examples are the same as that of application example 1, except that no relevant components are added.

[0071] Performance Test-1 Capillary Permeability Reduction Test

[0072] Experimental method: The cell line used was human skin capillary endothelial cells (BNCC337860, Beina Biotech), and the test conditions were: incubator temperature 37±1°C, saturated humidity, carbon dioxide 5±1%; cell culture and treatment were performed according to the grouping, and then the test was performed. The specific test was the determination of the tight junction protein Claudin-1 content. The test method was as follows:

[0073] (1) The cell suspension was inoculated into a 96-well plate at a culture density of 2000 cells / well. 100 μL of endothelial cell culture medium (BNCC364799, Beina Biotech) was added to each well. After culturing for 24 hours, the supernatant was discarded.

[0074] (2) 100 μL of endothelial cell culture medium was added to the blank control group, and 100 μL of culture medium containing 0.1% of the anti-aging composition of the embodiment and the comparative example was added to the sample group. After culturing for 24 hours, the cells were collected and washed 3 times with PBS buffer. 50 μL of RIPA cell lysis buffer containing protease inhibitors was added to each well. The lysed samples were centrifuged at 12000 g for 5 minutes, and the supernatant was collected.

[0075] Determination of tight junction protein Claudin-1 content: Human claudin-1 ELISA kit (Cat. No. BLL106520E, Baililai Biotechnology) was used to detect the Claudin-1 protein content in the cell supernatant of each group.

[0076] Tight junction is the main connection mode of capillary endothelial cells, regulating the permeability of capillaries. The higher the expression level of tight junction protein Claudin-1 after sample treatment, the lower the permeability of capillaries and the better the effect of reducing edema. The effect of the anti-aging composition in reducing edema is represented by the increase rate of tight junction protein Claudin-1 in human capillary endothelial cells, and the formula is as follows:

[0077] Claudin-1 increase rate = (Claudin-1 content in sample group / Claudin-1 content in blank group -1)*100%; the test results are shown in Table 5.

[0078] Table 5

[0079]

[0080]

[0081]

[0082] It can be seen from the above table that the anti-aging composition prepared in the embodiment of the present invention has a Claudin-1 increase rate of more than 50%, and has a good effect of reducing edema.

[0083] From the comparison of Examples 1-11, it can be seen that the weight ratio of ruscosaponin to neoruscosaponin in the Ruscus spp. extract will further affect the performance of the anti-aging composition prepared. When the weight ratio of ruscosaponin to neoruscosaponin is 1:(0.2-2.5), the Claudin-1 increase rate is 50%.

[0084] When the weight ratio of ruscosaponin and neoruscosaponin is 1:(0.9-1.2), the increase rate of Claudin-1 is more than 80%.

[0085] From the comparison of Examples 12-16, it can be seen that the weight of the components in the anti-aging composition will affect the performance of the product. When the Ruscus aconitifolia extract is 0.05-0.07 parts, the Coleus forskohlii extract is 0.2-0.3 parts, and the μ

[0086] - 0.1-0.2 parts of conopeptide and 0.5-1 parts of fermentation product of Lactobacillus crispatus, the anti-aging composition has a better effect of reducing edema.

[0087] Performance Test-2 Human skin patch test.

[0088] 30 volunteers were recruited, 15 males and 15 females, aged 20-50 years old. The closed patch test method was used. An equal amount (0.020mL-0.025mL) of the test sample (eye cream prepared by the application example, comparative application example and blank application example) was placed in a specific patch tester, which was applied to the volunteer's arm with a low-allergenic tape, and gently pressed to evenly apply it on the skin for 24 hours; the blank control group was distilled water. After 24 hours, the patch tester was removed, and the skin reaction was observed after 0.5 hours, 24 hours, and 48 hours, and the results were recorded. The level of adverse reactions to the skin is shown in Table 6 below.

[0089] Table 6

[0090]

[0091]

[0092] After testing, the eye creams prepared by the application examples of the present invention, the comparative application examples and the blank application examples all showed negative reactions after human patch tests, and were safe and non-irritating to human skin.

[0093] Performance Test-3 Human Efficacy Test.

[0094] Asian adult test subjects aged 28-60 with sensitive skin and structural dark circles and fine lines around the eyes were selected and randomly divided into 25 groups, with 5 people in each group. The volunteers gently applied the samples to the eyes once in the morning and evening (the eye cream prepared by application examples 1-18, comparative application examples 1-6 and blank application examples, and should not stay up late during use. The data were collected before use (T0), 30 minutes after use (T30min) and 28 days (T28);

[0095] After the visit, the volunteers washed their faces with facial cleanser and sat quietly in an air-conditioned room at a temperature of 21±1°C and a humidity of 50±10% for 2 hours. The dark circles and fine lines around the eyes were photographed and analyzed using VISIA-CR, and the elasticity tester Cutometer (MPA580, Courage and Khazaka, Germany) was used to measure the fundus skin firmness parameter F4 and skin elasticity parameter R2.

[0096] The effect of reducing structural dark circles is represented by the improvement of dark circle volume, the effect of smoothing wrinkles is represented by the improvement of eye fine lines area, the skin tightening effect is represented by the improvement of the firmness F4 value, and the effect of increasing skin elasticity is represented by the improvement of the elasticity parameter R2 value. The formula is as follows:

[0097] Dark circle volume, eye fine lines area, F4 value improvement rate = (T0-Tx) / T0*100%;

[0098] R2 value improvement rate = (Tx-T0) / T0*100%;

[0099] The results are shown in Table 7.

[0100] Table 7

[0101]

[0102]

[0103] It can be seen from the above table that the eye cream prepared by the application example of the present invention has a good effect of enhancing skin firmness and elasticity, fading structural dark circles and smoothing fine lines around the eyes.

[0104] Figure 1 These are the dark circle improvement pictures of volunteers before using eye cream, 30 minutes after using eye cream, and 28 days after using eye cream for the blank application example, application example 1, and application example 3; among them, (a) is the picture of dark circles before use, (b) is the picture of dark circles after using eye cream for 30 minutes, and (c) is the picture of dark circles after using eye cream for 28 days.

[0105] From the comparison of Application Examples 1-11, it can be seen that the weight ratio of ruscosaponin and neoruscosaponin in the Butcher's Pear extract will further affect the performance of the prepared anti-aging composition. When the weight ratio of ruscosaponin and neoruscosaponin is 1:(0.9-1.2), the effects of enhancing skin firmness and elasticity, fading structural dark circles and smoothing fine lines around the eyes are better.

[0106] From the comparison of Application Examples 12-16, it can be seen that the weight parts of the components in the anti-aging composition will affect the performance of the product. When 0.05-0.07 parts of Ruscus aconitifolia extract, 0.2-0.3 parts of Coleus forskohlii extract, 0.1-0.2 parts of μ-conopeptide, and 0.5-1 parts of Lactobacillus crispatus fermentation product are used, the prepared eye cream can enhance skin firmness and elasticity, and has better effects in fading structural dark circles and smoothing fine lines around the eyes.

[0107] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. An anti-aging composition, characterized in that: The invention comprises the following components in parts by weight: 0.01-0.2 parts of Ruscus aconitifolia extract, 0.01-1 parts of Coleus forskohlii extract, 0.01-2 parts of μ-conopeptide, and 0.01-3 parts of fermentation product of Lactobacillus crispatus; the Ruscus aconitifolia extract comprises ruscosaponin C 27 H 42 O4 and neoruscosapogenin C 27 H 40 O4.

2. The anti-aging composition according to claim 1, characterized in that The invention comprises the following components in parts by weight: 0.02-0.1 parts of Ruscus aconitifolia extract, 0.05-0.5 parts of Coleus forskohlii extract, 0.05-0.5 parts of μ-conopeptide and 0.1-2 parts of fermentation product of Lactobacillus crispatus.

3. The anti-aging composition according to claim 1, characterized in that The invention comprises the following components in parts by weight: 0.05-0.07 parts of Ruscus aconitifolia extract, 0.2-0.3 parts of Coleus forskohlii extract, 0.1-0.2 parts of μ-conopeptide and 0.5-1 parts of fermentation product of Lactobacillus crispatus.

4. The anti-aging composition according to claim 1, characterized in that In the Ruscus aconitifolia extract, the weight ratio of ruscosapogenin to neoruscosapogenin is 1:(0.2-2.5).

5. The anti-aging composition according to claim 1, characterized in that In the Ruscus aconitifolia extract, the weight ratio of ruscosapogenin to neoruscosapogenin is 1:(0.7-1.4); preferably, in the Ruscus aconitifolia extract, the weight ratio of ruscosapogenin to neoruscosapogenin is 1:(0.9-1.2).

6. The anti-aging composition according to claim 1, characterized in that: The preparation method of the Butcher's Broom extract is as follows: (1) grinding and crushing the leaves and / or rhizomes of Ruscus rubra and / or roots of Ruscus rubra, sieving to obtain a coarse powder, acid hydrolyzing, reflux extraction, and filtering to obtain a filtrate; (2) extracting the filtrate and centrifuging it, collecting the organic phase, washing it with alkali and drying it to powder to obtain the Ruscus aculeatus extract.

7. The anti-aging composition according to claim 1, characterized in that In the step (1), the weight ratio of the Ruscus aconitifolia leaves to the Ruscus aconitifolia rhizomes is 1:(0.5-5); and / or, the weight ratio of the Ruscus aconitifolia leaves to the Ruscus aconitifolia roots is 1:(2-7).

8. The anti-aging composition according to claim 1, characterized in that In the step (1), after grinding and pulverizing, the product is sieved through an 80-200 mesh sieve, and the mass ratio of the coarse powder to the solvent is 1 g: (40-60) mL; the temperature of the reflux extraction is 70-90° C., and the time of the reflux extraction is 2-4 h; in the step (2), the extraction solvent is at least one of n-hexane and dichloromethane, the volume ratio of the extraction solvent to the filtrate is (1-2): 1, the centrifugal speed is 1500-3000 rpm, and the centrifugal time is 1-5 min; the alkali for the alkali washing is sodium hydroxide, and the weight ratio of the alkali solution for the alkali washing to the organic phase is 1: (3-6); and the drying is carried out in a nitrogen environment at 50-65° C.

9. Use of the anti-aging composition according to any one of claims 1 to 8 in the preparation of skin products.

10. A skin care product, characterized in that: The skin care product comprises the following components in percentage by weight: 1-20% of the anti-aging composition according to any one of claims 1-8, 5-40% of a cosmetic base, and the balance being water; the cosmetic base comprising at least one of a thickener, a moisturizer, an emulsifier, a preservative, and a pH adjuster.

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