Production method of synthetase enzyme organic fertilizer

Abstract

The invention discloses a production method of a synthetase enzyme organic fertilizer. The production method comprises the following steps: carrying out precipitation and aeration treatment on biogas slurry, sterilizing and filtering, carrying out mixed fermentation on the biogas slurry, a microbial agent, a biological enzyme, molasses and organic acid, and filtering and homogenizing after fermentation to obtain a multi-enzyme enzyme organic fertilizer. According to the invention, resource utilization of the biogas slurry is realized, the production cost of the organic fertilizer is reduced, the source of the organic fertilizer is enriched, and the negative influence of the biogas slurry on the environment is reduced.

Description

Technical Field

[0001] The invention belongs to the technical field of organic fertilizer production, and in particular relates to a method for producing a synthetase enzyme organic fertilizer. Background Art

[0002] Biogas slurry is the liquid residue of anaerobic fermentation in biogas projects. Its pH is neutral or slightly alkaline. The composition characteristics of biogas slurry vary greatly due to the fermentation raw materials and treatment processes, but they all contain rich macronutrients such as nitrogen, phosphorus, potassium, micronutrients such as Ca, Fe, Zn, Cu, and rich microbial metabolites such as amino acids, vitamins, active enzymes, hormones, etc. Biogas slurry is considered to be a "biological pesticide" with no pollution, no residual toxicity, and no drug resistance, which can prevent and control plant diseases and insect pests; the large amount of nutrients such as nitrogen, phosphorus, and potassium contained in biogas slurry can improve soil fertility, the organic matter such as humic acid contained in biogas slurry can promote the formation of soil aggregates, and the biologically active substances contained in biogas slurry can enhance the activity of microorganisms and enzymes, thereby regulating the properties of soil and improving the growth environment of plants. Therefore, biogas slurry has been widely used in agriculture, forestry, animal husbandry, and fishery, specifically in biogas slurry fertilizer, biogas slurry seed soaking, biogas slurry biological pesticides, biogas slurry feed addition, and biogas slurry soilless cultivation nutrient solution, etc. It is a direct and low-cost resource utilization model.

[0003] However, studies have found that biogas slurry contains a large number of microorganisms and even harmful microorganisms. Due to the influence of raw materials, the heavy metal content of some biogas slurry samples is relatively high, and even exceeds the standard. The biogas slurry produced by anaerobic fermentation of poultry and livestock manure has a high technical detection rate of tetracyclines, antimicrobials and quinolone. When the biogas slurry is directly applied to soil, crops or poultry and livestock, it is in close contact with the food chain, posing potential risks to food safety, environmental and ecological safety.

[0004] Chinese patent CN105294216A discloses a biogas slurry drip irrigation fertilizer, comprising 90% concentrated biogas slurry, 1-1.5% urea, 1.2-3% monoammonium phosphate, 1.5-5% potassium chloride, 0.1-0.2% potassium dihydrogen phosphate, and the remainder being trace elements; it has the advantages of good fertilizer efficiency, not easy to clog the dripper head, and low production cost, but it is a mixed fertilizer of organic fertilizer and chemical fertilizer, and there are still potential risks such as soil compaction when the amount of chemical fertilizer is large and direct use of biogas slurry.

[0005] Chinese patent CN106747940A discloses a biogas slurry composite bacterial agent microbial liquid fertilizer and a preparation method thereof. The composition formula is as follows: every 1kg of the biogas slurry composite bacterial agent microbial liquid fertilizer contains 30g of Bacillus subtilis LB culture medium fermentation liquid, 30g of Bacillus licheniformis LB culture medium fermentation liquid, 20g of Pseudomonas LB culture medium fermentation liquid and 20g of Lactobacillus plantarum LB culture medium fermentation liquid; 500g of biogas slurry, 200g of urea, 120g of NH4H2PO4, 30g of KH2PO4, 35g of K2SO4, 9g of MgSO4, 5g of ethanol pure paste of Stellera chamaejasme, and 1g of Tween-80. The microbial liquid fertilizer contains unique beneficial bacteria and antibacterial components of traditional Chinese medicine, has the functions of improving soil, preventing soil-borne diseases and promoting crop growth, has low production cost and high product added value, and solves the problems of harmlessness and reuse of biogas slurry. However, it needs to be fermented separately to obtain fermentation broths of different strains of bacteria, the production process is relatively complicated, and there are still safety risks when the biogas slurry is directly used to prepare fertilizer. Summary of the invention

[0006] In view of the above technical problems, the present invention provides a method for producing a synthetase enzyme organic fertilizer, which realizes the resource utilization of biogas slurry, while reducing the production cost of organic fertilizer, enriching the source of organic fertilizer, and reducing the negative impact of biogas slurry on the environment.

[0007] In order to achieve the above object, the present invention provides a method for producing a synthetase enzyme organic fertilizer, comprising the following steps: (1) Biogas slurry pretreatment: Collect the clear liquid in the upper part of the fermentation product of the biogas tank, and precipitate and aerate it to obtain biogas slurry; (2) Sterilization and filtration: sterilize the biogas slurry and filter it, and retain the filtrate; (3) Fermentation: The filtrate is mixed with microbial agents, molasses, organic acids and biological enzymes for fermentation to obtain a fermentation liquid; (4) The fermentation liquid is filtered and homogenized to obtain multi-enzyme organic fertilizer.

[0008] Preferably, the biogas digester fermentation product in step (1) is obtained by fermenting one or more of poultry and livestock manure, straw, food processing waste and kitchen waste; the aeration time is 1-2 hours, and the gas-water ratio is 20-40.

[0009] Preferably, the sterilization method in step (2) is ultraviolet sterilization, and the power of the ultraviolet rays is 1400-2500W.

[0010] Preferably, the mass ratio of the filtrate in step (3) to the microbial agent, molasses, organic acid and biological enzyme is 1000:0.5-1:50-70:1-5:10-30.

[0011] Further preferably, the microbial agent comprises complex Bacillus and actinomycetes; the complex Bacillus is ≥ 10 billion / g; the actinomycetes is ≥ 10 billion / g.

[0012] More preferably, the composite Bacillus includes one or more of Bacillus subtilis, Bacillus megaterium and Bacillus coagulans.

[0013] Further preferably, the biological enzyme includes one or more of cellulase, protease, saccharification enzyme, complex polysaccharidase and coenzyme; the complex polysaccharidase includes glucanase and chitosanase; and the coenzyme is a B-family coenzyme.

[0014] More preferably, the enzymatic activities of the cellulase and coenzyme are ≥8000 U / g; the enzymatic activities of the saccharifying enzyme and complex polysaccharidase are ≥500 U / g; and the enzymatic activity of the protease is ≥300 U / g.

[0015] Further preferably, the organic acid is one or more of humic acid, alginic acid and indolebutyric acid.

[0016] Preferably, the fermentation condition in step (3) is 25-35° C. for 5-10 days.

[0017] The beneficial effects of the present invention are: 1. Make full use of the rich macronutrients such as nitrogen, phosphorus, potassium and trace nutrients such as zinc in the biogas slurry. It also contains cellulose, cellulase, protein, ribose, auxin, gibberellin, unsaturated fatty acids and certain antibiotic substances and minerals. The related substances remaining after fermentation are still retained in the multi-enzyme organic fertilizer. When applied to farmland, it can be used as a fast-acting nutrient to supply the nutrients needed for plant growth, thereby promoting plant growth.

[0018] 2. By using sedimentation, aeration, ultraviolet sterilization and fermentation technology, harmful microorganisms in the biogas slurry are killed, and harmful and odorous gases are degraded, reducing the impact on the environment. When it is prepared into multi-enzyme organic fertilizer for use, it can also avoid the harmful microorganisms in the biogas slurry from affecting crop production and reduce the incidence rate.

[0019] 3. The present invention realizes the resource utilization of biogas slurry with a simple process. It can decompose and degrade harmful substances in biogas slurry while retaining beneficial elements, and also reduces the production cost of organic fertilizer and enriches the source of organic fertilizer. DETAILED DESCRIPTION

[0020] The technical solution of the present invention is further explained below in conjunction with specific embodiments. It is worth noting that the following embodiments are only preferred embodiments of the present invention and should not be construed as limiting the present invention. The protection scope of the present invention shall be subject to the contents recorded in the claims. Any modification or replacement made by a person skilled in the art to the technical solution of the present invention without creative work shall fall within the protection scope of the present invention.

[0021] Example 1 (1) Biogas slurry pretreatment: The clear liquid from the middle and upper parts of the biogas digester of the pig farm was taken as the raw material, and after settling for 3 days, it was aerated for 1 hour to obtain biogas slurry; the aeration gas-water ratio was 30; (2) Sterilization and filtration: Pass the biogas slurry into an ultraviolet sterilizer and irradiate it under a 2000W ultraviolet lamp for 30 minutes for sterilization. Then filter it through a 20-mesh sieve and retain the filtrate. (3) Preparation of additives: Bacillus subtilis, Bacillus megaterium and Bacillus coagulans are mixed in equal mass ratios to obtain a composite Bacillus, so that the effective viable count is ≥ 10 billion / g; The composite bacillus and actinomycetes are mixed to obtain a microbial agent, so that the effective viable bacteria count is ≥ 20 billion / g; The glucanase and chitosanase are mixed to obtain a composite glucanase, which is then mixed with cellulase, protease, saccharifying enzyme and B-family coenzyme to obtain a biological enzyme, so that the biological enzyme activity is ≥9000 U / g; Take humic acid, alginic acid and indolebutyric acid in equal molar ratios and mix them to obtain organic acid (4) mixing the filtrate, microbial agent, molasses, organic acid and biological enzyme in a mass ratio of 1000:0.8:60:3:20, and fermenting at 30° C. for 6 days to obtain a fermentation liquid; (5) The fermentation liquid is passed through a 40-mesh sieve and then homogenized to obtain a multi-enzyme organic fertilizer.

[0022] Example 2 The method and steps are the same as those in Example 1, except that the gas-water ratio in step (1) is changed to 20; In step (2), the power of the UV lamp is changed to 1400W; In step (4), the mass ratio of the filtrate, microbial agent, molasses, organic acid and biological enzyme is changed to 100:0.5:50:1:10, and multi-enzyme organic fertilizer is obtained by fermentation.

[0023] Example 3 The method and steps are the same as in Example 1, except that the gas-water ratio in step (1) is changed to 40; In step (2), the power of the UV lamp is changed to 2500W; In step (4), the mass ratio of the filtrate, microbial agent, molasses, organic acid and biological enzyme is changed to 100:1:70:5:30, and multi-enzyme organic fertilizer is obtained by fermentation.

[0024] Comparative Example 1 The method and steps are the same as those in Example 1, except that the sedimentation and aeration step in step (1) is omitted to prepare an organic fertilizer.

[0025] Comparative Example 2 The method and steps are the same as those in Example 1, except that the ultraviolet sterilization step in step (2) is omitted to prepare an organic fertilizer.

[0026] Comparative Example 3 The method and steps are the same as those in Example 1, except that no organic acid is added in step (4), thereby preparing an organic fertilizer.

[0027] Comparative Example 4 The method and steps are the same as those in Example 1, except that no biological enzyme is added in step (4), thereby preparing an organic fertilizer.

[0028] Comparative Example 5 The method and steps are the same as those in Example 1, except that all the microbial agents in step (4) are replaced with Bacillus subtilis to prepare an organic fertilizer.

[0029] Comparative Example 6 The method and steps are the same as those in Example 1, except that all the microbial agents in step (4) are replaced with actinomycetes to prepare an organic fertilizer.

[0030] Results: The organic fertilizer prepared in the above examples and comparative examples was diluted 200 times and sprayed on the citrus field. The amount of organic fertilizer was 20 kg / mu and the frequency of use was once a month. The soil organic matter content, the comprehensive incidence of citrus trees, the citrus yield and the Cu content in the soil of the citrus orchard were statistically analyzed one year after fertilization. The results are as follows:

[0031] The results showed that the multi-enzyme organic fertilizer obtained by mixing and fermenting the biogas slurry with microbial agents, molasses and other substances after sedimentation, aeration and ultraviolet sterilization can significantly increase the soil organic matter content. Compared with the direct application of biogas slurry, it significantly reduced the Cu content enriched in the soil, and the yield and incidence of citrus can be on par with commercial compound fertilizers.

Claims

1. A method for producing a synthetase enzyme organic fertilizer, characterized in that: The steps include: (1) Biogas slurry pretreatment: Collect the clear liquid in the upper part of the fermentation product of the biogas tank, and precipitate and aerate it to obtain biogas slurry; (2) Sterilization and filtration: sterilize the biogas slurry and filter it, and retain the filtrate; (3) Fermentation: The filtrate is mixed with microbial agents, molasses, organic acids and biological enzymes for fermentation to obtain a fermentation liquid; (4) The fermentation liquid is filtered and homogenized to obtain multi-enzyme organic fertilizer.

2. The method for producing a synthetic enzyme organic fertilizer according to claim 1, characterized in that: The biogas digester fermentation product in step (1) is obtained by fermenting one or more of poultry and livestock manure, straw, food processing waste and kitchen waste; the aeration time is 1-2 hours, and the gas-water ratio is 20-40.

3. The method for producing a synthetase enzyme organic fertilizer according to claim 1, characterized in that: The sterilization method described in step (2) is ultraviolet sterilization, and the power of the ultraviolet rays is 1400-2500W.

4. The method for producing a synthetase enzyme organic fertilizer according to claim 1, characterized in that: The mass ratio of the filtrate in step (3) to the microbial agent, molasses, organic acid and biological enzyme is 1000:0.5-1:50-70:1-5:10-30.

5. The method for producing a synthetase enzyme organic fertilizer according to claim 4, characterized in that: The microbial agent comprises compound Bacillus and actinomycetes; the compound Bacillus is ≥10 billion / g; the actinomycetes is ≥10 billion / g.

6. The method for producing a synthetase enzyme organic fertilizer according to claim 5, characterized in that: The complex Bacillus includes one or more of Bacillus subtilis, Bacillus megaterium and Bacillus coagulans.

7. The method for producing a synthetase enzyme organic fertilizer according to claim 4, characterized in that: The biological enzyme includes one or more of cellulase, protease, saccharifying enzyme, complex polysaccharidase and coenzyme; the complex polysaccharidase includes glucanase and chitosanase; and the coenzyme is a B-family coenzyme.

8. The method for producing a synthetase enzyme organic fertilizer according to claim 7, characterized in that: The enzymatic activities of the cellulase and coenzyme are ≥8000 U / g; the enzymatic activities of the saccharifying enzyme and complex polysaccharidase are ≥500 U / g; and the enzymatic activity of the protease is ≥300 U / g.

9. The method for producing a synthetase enzyme organic fertilizer according to claim 4, characterized in that: The organic acid is one or more of humic acid, alginic acid and indolebutyric acid.

10. The method for producing a synthetase enzyme organic fertilizer according to claim 1, characterized in that: The fermentation conditions in step (3) are 25-35° C. for 5-10 days.

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