Simple 13CO2 pulse marking device and method with adjustable volume and height

Abstract

The invention relates to the technical field of stable isotope tracing, in particular to a simple 13CO2 pulse marking device with adjustable volume and height and a method thereof.The simple 13CO2 pulse marking device comprises an incubator, a preparation structure is arranged on one side of the incubator, a pulse marking structure is arranged above the incubator, and a detection structure is arranged on the side adjacent to the preparation structure; a circulation structure is arranged on the side, away from the preparation structure, of the detection structure, a rising structure is arranged at the bottom of the incubator, a plant culture base is arranged above the rising structure, a volume regulation and control structure is arranged above the plant culture base, and an exhaust structure is arranged on the side, away from the detection structure, of the incubator; through organic integration of all the structures, the whole process from plant culture to pulse marking can be achieved in the same device, all the parts are matched with one another, the convenience and efficiency of experimental operation are greatly improved, errors and inconvenience caused by device conversion are reduced, and the experimental efficiency is improved. And an integrated solution is provided for accurately researching plants in different growth stages and related physiological processes.

Description

Technical Field

[0001] The present invention relates to the field of stable isotope tracing technology, and in particular to a simple and easy-to-use device with adjustable volume and height. 13 CO2 pulse labeling device and method. Background Art

[0002] In the research scope of plant physiology and ecology, plants can convert carbon dioxide (CO2) in the atmosphere into photosynthetic products through the key physiological process of photosynthesis, of which about 30% of the photosynthetic products are transported to the underground part in the form of root secretions. In addition to a part of the photosynthetic products contained in these root secretions being fixed by the soil and the microorganisms therein, they mainly provide a source of material and energy for the life activities of microorganisms, thereby driving the material conversion process in the soil to meet the needs of plants for various nutrients during growth and development. Therefore, in-depth exploration of the distribution and laws of plant photosynthetic products in different soil microbial populations and carbon pools is of extremely critical scientific significance for deeply revealing the internal mechanism of plants regulating the root microbiome with the help of root secretions, and accurately clarifying the impact of increased CO2 concentrations on soil carbon pools under climate change conditions.

[0003] At present, in the technical field of isotope CO2 pulse labeling of plants, the simple sealed box method is a commonly used method. The device used in this method is mainly composed of a transparent airtight box, a fan, a gas valve and other components. In actual application, a concave water tank seal at the bottom is usually used to ensure the airtightness of the box, and the CO2 concentration in the box is monitored in real time with the help of an external far-infrared CO2 monitor. The isotope CO2 is replenished in time according to the monitored concentration data, so that the CO2 concentration in the box can be maintained within a pre-set specific range. In order to adapt to different plant species, especially for the labeling needs of crops with higher plant heights (such as corn, sorghum, etc.), it is often necessary to specially make a large-volume independent sealed box to broaden the application range of a single device; and in order to obtain a more ideal labeling effect, when using such a device for labeling experiments, it is often necessary to use a large amount of isotope carbon dioxide to ensure the stability of the CO2 concentration in the box.

[0004] Obviously, the existing marking device has significant defects: first, the volume and height of the box are usually fixed, which means that the device can only be used for marking experiments of specific plant species or specific growth periods, and lacks flexibility; second, the water tank seal method is adopted, and when the air pressure inside the device changes, gas leakage is very likely to occur, which will not only lead to a significant reduction in the utilization rate of carbon dioxide, but also have an adverse effect on the accuracy of the marking experiment. These shortcomings have greatly limited the application scenarios of the device and weakened the marking effect. In addition, given the high price of stable isotopes, the large-scale use of isotope carbon dioxide will also greatly increase the cost of the experiment, thus bringing many inconveniences and economic pressures to related research work.

[0005] The patent document with the publication number "CN108307926A" discloses a field in-situ continuous low-abundance 13CO2 isotope labeling device and method, which aims to solve the problems of low labeling accuracy and high cost in the traditional C element labeling device or method for plants using a pulsed labeling method. The labeling box of the invention is designed as a transparent box with a through hole in the side wall. The combustion end of the burner passes through the through hole of the labeling box and extends into the interior of the labeling box. The end of the burner is connected to the gas outlet of the liquefied gas cylinder and the gas outlet of the oxygen cylinder at the same time, and an electronic igniter is provided at the combustion end of the burner; the fan is fixed in the middle of the top of the labeling box, the air conditioner is fixed on the upper part of the side wall of the labeling box, the CO2 measurement and control device and the humidifier are located in the labeling box, and the dehumidifier is fixed on the lower part of the side wall of the labeling box. The fan, air conditioner, CO2 measurement and control device, electronic igniter, dehumidifier and humidifier are coordinated and controlled by an automatic control system, so as to achieve continuous low-abundance labeling of crops. 13 Purpose of CO2 labeling.

[0006] Nevertheless, the invention still has many limitations: its overall structure is relatively fixed, and the size, shape and other parameters of the marking box are determined after the design is completed, which makes it difficult to flexibly adjust according to the actual needs of various crops at different growth stages and different plant sizes. For example, for crops with high plant height or large crown width in the late growth stage, the crops may not grow normally due to the limited space of the marking box, which seriously affects the marking effect;

[0007] In addition, the invention uses a burner to burn liquefied gas and oxygen to obtain low-abundance 13 CO2, in the maintenance labeling box 13 In the process of adjusting the CO2 concentration, it is difficult to ensure the complete combustion process and the gas diffusion phenomenon is difficult to effectively control, which easily leads to the problem of low gas utilization efficiency; at the same time, the continuous combustion process requires the consumption of a large amount of liquefied gas and oxygen, which will undoubtedly significantly increase the cost of use and bring an economic burden to the long-term development of the experiment;

[0008] In addition, the marking box of the invention adopts a bottom-opening design. Although it is convenient to place it on the soil where the crops to be marked are planted, this design makes it difficult to effectively ensure its air tightness. The open bottom may cause outside air to enter the box more easily or the internal gas to leak more easily, thereby affecting the temperature and humidity precisely controlled in the marking box. 13 Environmental parameters such as CO2 concentration cause serious interference, greatly reducing the accuracy and reliability of labeling.

[0009] Therefore, the existing 13 CO2 pulse labeling devices generally have problems such as insufficient device flexibility, limited gas supply control, poor airtightness and environmental stability, which are difficult to meet the current research on the distribution law of plant photosynthetic products and related ecological experiments for high-precision, high-efficiency, low-cost isotope labeling devices. It is urgent to develop a new type of 13 CO2 pulse marking device is used to overcome the above defects. Summary of the invention

[0010] In order to solve the problems of insufficient flexibility, limited gas supply control, poor air tightness and environmental stability in the prior art, the present invention provides a simple and adjustable volume and height 13 The CO2 pulse labeling device and method can meet the needs of nutritional labeling in different growth and development stages of plants.

[0011] The first technical solution of the present invention: simple and easy to adjust volume and height 13 The CO2 pulse marking device comprises an incubator, wherein a preparation structure is arranged on one side of the incubator, a pulse marking structure is arranged above the incubator, a detection structure is arranged on the side adjacent to the preparation structure, a circulation structure is arranged on the side of the detection structure away from the preparation structure, an ascending structure is arranged at the bottom of the incubator, a plant culture medium base is arranged above the ascending structure, a volume control structure is arranged above the plant culture medium base, and an exhaust structure is arranged on the side of the incubator away from the detection structure; a storage tank is provided at the bottom edge of the incubator, a simulated soil plate is provided on the inner side of the storage tank, and a culture basket is provided on the inner side of the simulated soil plate; a light shielding cover is detachably connected to the upper side of the culture basket; a bent partition is detachably connected to the upper side of the light shielding cover; a connecting pipe is provided on one side of the simulated soil plate, one end of the connecting pipe is connected to the storage tank, and the end of the connecting pipe away from the storage tank is connected to the culture basket; a conveying pipe is provided on the side of the incubator away from the connecting pipe.

[0012] In the present invention, the incubator is used as a core bearing component, one side of which is equipped with a preparation structure for material preparation and blending, and a pulse labeling structure is arranged above for pulse labeling experimental operation. A detection structure is placed on one side adjacent to the preparation structure, and a circulation structure is arranged on the opposite side. The circulation structure is connected to the inside of the incubator through a pipeline to realize material circulation. An ascending structure is arranged at the bottom of the incubator, and the plant culture medium seat above the ascending structure is used to carry plants. The volume control structure above the plant culture medium seat can adjust the internal volume of the incubator, and an exhaust structure is arranged on the other side of the incubator to control the gas discharge. A storage tank is provided at the bottom edge of the incubator, and the simulated soil plate in the storage tank and the culture basket inside the storage tank provide a simulated soil environment for plant growth. The light shielding cover above the culture basket can adjust the light, and the bent partition above the light shielding cover plays a role of separation and guidance. The connecting pipe on one side of the simulated soil plate connects the storage tank and the culture basket, and the conveying pipe on the other side of the incubator is connected to the preparation structure. The present invention can realize the whole process from plant cultivation to pulse labeling in the same device through the organic integration of various structures. The various components cooperate with each other, which greatly improves the convenience and efficiency of experimental operation and reduces the errors and inconveniences caused by device conversion or component dispersion. 13 It provides an integrated solution for CO2 labeling related physiological processes.

[0013] Preferably, the preparation structure includes a two-way valve, which is connected to the end of the delivery tube away from the incubator, the end of the two-way valve away from the delivery tube is detachably connected to a side tube, the end of the side tube away from the two-way valve is fixedly connected to a first conical flask, the top of the first conical flask is detachably connected to a guide tube, the end of the guide tube away from the first conical flask is provided with a second conical flask, a diverter valve is provided in the middle of the guide tube, a control tube is provided at the top of the diverter valve, and an electromagnetic valve is provided at the end of the control tube away from the diverter valve.

[0014] The two-way valve in the present invention can flexibly switch the flow direction of gas or liquid to meet the requirements of material transmission direction in different experimental stages. The first conical flask and the second conical flask can store different substances respectively. Through the connection of the guide tube and the control of the diverter valve, the distribution ratio of the substance can be accurately adjusted, and the solenoid valve can accurately control the on and off of the substance, thereby realizing the accurate preparation and supply of gas and liquid required for plant growth, ensuring that plants can obtain a suitable material environment at different growth periods, improving the stability and repeatability of the plant growth state in the experiment, and contributing to in-depth research on the physiological response of plants under different material supply conditions.

[0015] Preferably, the circulation structure includes a first circulation pipe, one end of which is connected to the culture basket, the first circulation pipe passes through the simulated soil plate and the culture box, a control valve is provided at the end of the first circulation pipe away from the culture basket, a second circulation pipe is provided on one side of the control valve, a feed box is provided at the end of the second circulation pipe away from the control valve, an environmental simulation circulation machine is provided on the side of the feed box away from the second circulation pipe, a discharge box is provided on the bottom side of the environmental simulation circulation machine, a third circulation pipe and a fourth circulation pipe are provided on the side of the discharge box away from the environmental simulation circulation machine, and one end of the third circulation pipe and the fourth circulation pipe are connected to the culture basket away from the discharge box.

[0016] In the present invention, one end of the first circulation pipe is connected to the culture basket and passes through the simulated soil plate and the culture box, and the other end is connected to the control valve. One side of the control valve is connected to the second circulation pipe to the feed box. An environmental simulation circulation machine is arranged on one side of the feed box, and the discharge box at the bottom is connected to the third circulation pipe and the fourth circulation pipe and finally returns to the culture basket. The present invention can make the gas or liquid flow stably in the entire circulation system through the drive of the environmental simulation circulation machine. The first circulation pipe, the second circulation pipe, the third circulation pipe and the fourth circulation pipe constitute a complete circulation path to ensure that the substance is evenly distributed in the plant growth environment. The control valve can flexibly adjust the circulation path and flow rate according to the experimental requirements, effectively maintain the stability and uniformity of the material circulation in the plant growth environment, simulate the material circulation process in the natural environment, and enable plants to grow under conditions closer to the real ecological conditions, which is conducive to obtaining more reliable experimental data and is convenient for accurately studying the effects of plants under specific environmental circulation conditions. 13 Physiological processes such as absorption and conversion of CO2.

[0017] Preferably, the detection structure includes a laser transmitter, which is arranged on one side of the control valve, a laser receiver is arranged on the side of the control valve away from the laser transmitter, and the first circulation pipe is located between the laser transmitter and the laser receiver.

[0018] The present invention uses a laser transmitter to emit laser light and a laser receiver to receive laser light. Based on the attenuation and scattering of the laser light in the circulating material, the present invention can non-invasively monitor key parameters such as the flow rate, density, and composition uniformity of the circulating gas and liquid in real time. This detection method does not require direct contact with the circulating material, thus avoiding interference with the circulation system, and can quickly and accurately obtain a large amount of data, providing an accurate basis for timely adjustment of the operating parameters of the circulation structure, ensuring that the plant growth environment is always in an ideal state, improving the accuracy and reliability of the entire device's environmental control, and helping to accurately study the effects of environmental factors on plants. 13 The influencing mechanism of CO2 labeling process.

[0019] Preferably, a plurality of ventilation holes are provided on the side of the plant culture medium base.

[0020] The present invention provides a channel for gas exchange between plant roots and the surrounding environment. Plant roots can smoothly obtain oxygen and discharge waste gases such as carbon dioxide through these air exchange holes, ensuring the normal respiration of plant roots and promoting the overall growth and development of plants. 13 In the CO2 pulse labeling experiment, good root respiration helps to maintain the normal physiological metabolism of the plant, so that the experimental results can more truly reflect the response of the plant to isotope labeling under natural gas exchange conditions, thereby improving the accuracy and credibility of the experimental data.

[0021] Preferably, the rising structure includes a ventilation ring, which is fixedly connected to the bottom center of the culture basket, an air pipe is arranged on the side of the ventilation ring, an air pump is arranged on the end of the air pipe away from the ventilation ring, a first rising ring is slidably connected to the inner side of the ventilation ring, and a second rising ring is slidably connected to the inner side of the first rising ring; a ventilation groove is provided at the bottom end of the ventilation ring, and one end of the ventilation groove is connected to the air pipe.

[0022] In the present invention, the ventilation ring is fixed at the bottom center of the culture basket, and the side is connected to the air pipe and the air pump. The inner side of the ventilation ring is slidably connected to the first rising ring and the second rising ring, and the bottom end is provided with a ventilation groove that communicates with the air pipe. When the air pump is started, the gas enters the ventilation groove through the air pipe and fills the ventilation ring, thereby pushing the first rising ring and the second rising ring to rise, and then driving the plant culture base to move upward smoothly. This rising method can accurately send the plant culture base from the inside of the incubator upward into the pulse labeling structure, avoiding plant damage and environmental interference that may be caused by manual transfer, ensuring the accuracy and repeatability of experimental operations, and facilitating subsequent experiments in the pulse labeling structure. 13 CO2 pulse labeling experiments provide a reliable means of plant transfer to ensure the smooth progress of the experiment.

[0023] Preferably, a square hole is provided at the center of the bent partition, and the square hole is located directly above the plant culture medium base.

[0024] The present invention provides a precise channel for the plant culture base during the rising process. It ensures that the plant culture base can smoothly enter the pulse marking structure through the bent partition. At the same time, the setting of the square hole helps to form a relatively stable gas and temperature stratified environment in the incubator, reducing the mutual interference of environmental factors in different regions. This stable environment is conducive to the normal growth of plants in the incubator and the subsequent transfer operation to the pulse marking structure, improving the stability and reliability of the entire device in controlling the plant growth environment, and laying a good foundation for the successful implementation of the experiment.

[0025] Preferably, the volume control structure includes a reference plate, which is arranged above the bent partition, and one end of the reference plate is detachably connected to the incubator; a first push plate is arranged at the end of the reference plate away from the incubator, and one end of the first push plate is fixedly connected to the first sliding plate, and the end of the first sliding plate away from the first push plate is fixedly connected to a spring, and the end of the spring away from the first sliding plate is fixedly connected to a first slide rail, the first sliding plate is slidably connected to the first slide rail, and one side of the bottom of the first slide rail is detachably connected to the incubator; a second push plate is arranged at the side of the first push plate away from the first sliding plate, and the side of the second push plate away from the first push plate is fixedly connected to the second sliding plate, and one end of the second sliding plate away from the second push plate is fixedly connected to the first transmission column, and the first transmission column is away from the second sliding plate A first servo electric cylinder is provided at one end, and an output end of the first servo electric cylinder is fixedly connected to the first transmission column, a second slide rail is provided below the first servo electric cylinder, the second sliding plate is slidably connected to the second slide rail, and one side of the bottom of the second slide rail is detachably connected to the incubator; a third push plate is provided on one side of the second push plate, a side of the third push plate away from the second push plate is fixedly connected to the third sliding plate, a side of the third sliding plate away from the third push plate is fixedly connected to the third transmission column, a second servo electric cylinder is provided at one end of the third transmission column away from the third sliding plate, the output end of the second servo electric cylinder is fixedly connected to the third transmission column, a third slide rail is provided below the second servo electric cylinder, the third sliding plate is slidably connected to the third slide rail, and one side of the bottom of the third slide rail is detachably connected to the incubator.

[0026] The present invention is connected to the incubator through a reference plate, one end of which is connected to a first push plate, which is connected to a slide rail through a sliding plate and a spring to achieve elastic buffering; the second push plate is connected to a first servo electric cylinder and a slide rail through a sliding plate and a transmission column to achieve electric drive; the third push plate is connected to the second servo electric cylinder through a similar structure. The present invention can flexibly adjust the volume of the incubator according to the actual needs during plant growth.

[0027] Preferably, the exhaust structure includes a rotating plate, which is arranged on the side of the bent partition plate, a rotating column is fixedly connected to the side of the rotating plate close to the incubator, the side of the rotating column is rotatably connected to the incubator, a servo motor is arranged at the end of the rotating column away from the rotating plate, the output end of the servo motor is fixedly connected to the rotating column, a fixed plate is arranged below the servo motor, and one side of the fixed plate is fixedly connected to the incubator; a transmission plate is rotatably connected to one side of the rotating plate, the end of the transmission plate away from the rotating plate is rotatably connected to the vertical plate, the top of the vertical plate is rotatably connected to the first triangular plate, and the end of the first triangular plate away from the vertical plate is fixedly connected to the first sealed plate; the middle of the vertical plate is rotatably connected to the second triangular plate, and the end of the second triangular plate away from the vertical plate is fixedly connected to the second sealed plate; the bottom end of the vertical plate is rotatably connected to the third triangular plate, and the side of the third triangular plate away from the vertical plate is fixedly connected to the third sealed plate; both sides of the first sealed plate, the second sealed plate and the third sealed plate are rotatably connected to the incubator.

[0028] In the present invention, the rotating plate connects the rotating column and the servo motor, the rotating plate is connected to the vertical plate through the transmission plate, the vertical plates are respectively connected to the first triangular plate, the second triangular plate, and the third triangular plate, and the ends thereof are connected to the first sealed plate, the second sealed plate, and the third sealed plate and are rotatably connected to the incubator. The present invention uses a servo motor to accurately drive the rotating plate to rotate, and drives the vertical plate to move through the transmission plate, so that the first sealed plate, the second sealed plate, and the third sealed plate can work together to achieve precise exhaust control of the gas in the incubator. The present invention can accurately adjust the exhaust volume and exhaust timing according to experimental requirements, effectively maintain the stability of environmental parameters such as gas pressure and composition in the incubator, and avoid environmental fluctuations caused by improper exhaust on plant growth and 13 The design of multiple sealed plates improves the flexibility and accuracy of exhaust control, adapts to the exhaust requirements under different experimental conditions, and ensures the stability and reliability of the experimental environment.

[0029] Preferably, the pulse marking structure includes a bottom sealing seat, which is arranged above the incubator, and the bottom surface of the bottom sealing seat is detachably connected to the upper surface of the incubator, a first airtight tube is arranged above the bottom sealing seat, a second airtight tube is arranged above the first airtight tube, a third airtight tube is arranged above the second airtight tube, and an airtight upper cover is arranged above the third airtight tube.

[0030] The present invention constructs a pulse labeling space with good sealing performance. The various components are tightly connected through a specific connection method, which effectively prevents the leakage of isotope CO2 and ensures the accuracy and safety of the pulse labeling experiment. Different lengths of airtight tubes can be combined and selected according to the height of the plant to meet the pulse labeling needs of various plants, and to accurately study the pulse labeling of plants at different spatial scales. 13 The response of CO2 pulse labeling provides a reliable experimental environment, which helps to expand the scope of experimental objects and improve the diversity and representativeness of experimental data.

[0031] Preferably, a first circular hole is opened at the center of the bottom sealing seat, and a second circular hole is opened at the center of the top of the incubator. The centers of the first circular hole, the second circular hole and the plant culture medium seat are located on the same vertical line, and the sizes of the first circular hole, the second circular hole and the plant culture medium seat are adapted to each other.

[0032] The present invention ensures that the plant culture base can accurately pass through the top of the incubator and enter the pulse labeling structure during the rising process, thereby improving the accuracy and stability of the plant transfer process and reducing experimental errors or device failures caused by position deviation. This precise alignment design is beneficial to the entire 13 The smooth progress of the CO2 pulse labeling experiment ensured the consistency and reliability of the experimental operation and provided a basic guarantee for obtaining high-quality experimental data.

[0033] Preferably, the length of the first airtight tube is 200 mm and the outer wall thickness is 20 mm; the length of the second airtight tube is 400 mm and the outer wall thickness is 20 mm; the length of the third airtight tube is 700 mm and the outer wall thickness is 20 mm; the diameter of the bottom sealing seat, the first airtight tube, the second airtight tube and the third airtight tube is 300 mm.

[0034] The standardized size design of the present invention facilitates the manufacturing, assembly and maintenance of the device. The uniform diameter and thickness design ensures the tightness and structural strength of the connection between the components. The airtight tubes of different lengths provide a clear basis for selecting the appropriate pulse marking space according to the plant height, which is conducive to improving the reliability and stability of the device. 13 The consistency and repeatability of the experimental environment are ensured in the CO2 pulse labeling experiment, which reduces the experimental errors caused by differences in device components and improves the comparability and scientificity of the experimental data.

[0035] Preferably, the bottom edge of the bottom sealing seat is fixedly connected to a first flange, a first groove is provided on the inner side of the first flange, a first sealing gasket is arranged above the first groove, a second flange is detachably connected to the upper side of the first flange, the inner side of the second flange is fixedly connected to the bottom edge of the first airtight tube, a second groove is provided on the bottom surface of the second flange, and the upper surface of the first sealing gasket fits with the second groove; the top edge of the first airtight tube is fixedly connected to a third flange, a third groove is provided on the inner side of the third flange, a second sealing gasket is arranged above the third groove, a fourth flange is detachably connected to the upper side of the third flange, the inner side of the fourth flange is fixedly connected to the bottom edge of the second airtight tube, a fourth groove is provided on the bottom surface of the fourth flange, and the upper surface of the second sealing gasket fits with the fourth groove fit together; a fifth flange is fixedly connected to the top edge of the second airtight tube, a fifth groove is opened on the inner side of the fifth flange, a third sealing gasket is arranged above the fifth groove, a sixth flange is detachably connected to the top of the fifth flange, the inner side of the sixth flange is fixedly connected to the bottom edge of the third airtight tube, a sixth groove is opened on the bottom surface of the sixth flange, and the upper surface of the third sealing gasket fits together with the sixth groove; a seventh flange is fixedly connected to the top edge of the third airtight tube, a seventh groove is opened on the inner side of the seventh flange, a fourth sealing gasket is arranged above the seventh groove, an eighth flange is detachably connected to the top of the seventh flange, the inner side of the eighth flange is fixedly connected to the edge of the airtight upper cover, an eighth groove is opened on the bottom surface of the eighth flange, and the upper surface of the fourth sealing gasket fits together with the eighth groove.

[0036] The present invention utilizes the cooperation between flanges and sealing gaskets to effectively enhance the sealing of the connections between the components. The first flange and the second flange, the third flange and the fourth flange, the fifth flange and the sixth flange, and the seventh flange and the eighth flange are tightly connected through the card slot and the sealing gasket to prevent gas leakage from the connection gap. The present invention greatly improves the airtightness of the pulse marking structure, ensuring that the pulse marking structure is airtight during operation. 13 During the CO2 pulse labeling experiment, isotope CO2 will not leak, ensuring the stability and accuracy of the experimental environment, making the experimental data more reliable, providing an accurate basis for scientific research, and reducing experimental errors and data deviations caused by airtightness problems.

[0037] Preferably, an upper support plate is disposed above the second flange, the fourth flange and the sixth flange, and a lower support plate is disposed below the third flange, the fifth flange and the seventh flange.

[0038] The present invention enhances the structural stability of the airtight pipe connection. The upper support plate and the lower support plate can effectively prevent the connection from loosening or deformation due to pressure changes or external vibrations, further ensuring the airtightness of the pulse marking structure and the reliability of the overall structure, which is conducive to long-term 13 Maintain the normal operation of the device during the CO2 pulse labeling experiment, reduce experimental interruptions or data deviations caused by device structure problems, and ensure the smooth progress of the experiment and the accuracy of the data.

[0039] Preferably, a first straight airtight valve is provided on the side of the second airtight tube; a second straight airtight valve is provided on the side of the third airtight tube; and a third straight airtight valve is provided on the upper surface edge of the airtight upper cover.

[0040] The straight-through airtight valve in the present invention can be used to adjust the gas pressure, flow rate and composition in the pulse labeling structure. It provides a convenient control means when filling isotope CO2, balancing gas pressure or performing gas sampling, improves the flexibility and controllability of experimental operation, helps to accurately control the gas environment parameters during the pulse labeling experiment, ensures the accuracy and repeatability of the experimental results, and enables the experimental process to be accurately regulated according to research needs to meet the requirements of different experimental purposes.

[0041] Preferably, a membrane pressure gauge is provided at the center of the upper surface of the airtight upper cover; an aviation airtight power plug is provided on one side of the membrane pressure gauge, and the aviation airtight power plug is electrically connected to the airtight upper cover; an airtight three-way valve is provided on the side of the aviation airtight power plug away from the membrane pressure gauge; and a fan is provided on the bottom surface of the airtight upper cover.

[0042] The membrane pressure gauge of the present invention can monitor the air pressure changes in the pulse marking structure in real time, provide a basis for air pressure adjustment, and ensure that the experiment is carried out within a safe and stable air pressure range; the aviation airtight power plug provides a reliable power connection and is suitable for a variety of experimental environments; the airtight three-way valve facilitates gas in and out and channel switching, and is convenient for operations such as isotope CO2 charging and gas sampling; the fan can make the gas distribution in the pulse marking structure more uniform, improve the uniformity and accuracy of isotope CO2 labeling, and is conducive to obtaining more accurate experimental data, providing good experimental conditions for in-depth research on the absorption and utilization of isotope labels by plants.

[0043] Preferably, the diameter of the fan is 100 mm.

[0044] The fan diameter size design in the present invention is optimized to generate appropriate airflow intensity and circulation effect in the pulse labeling structure, which can ensure uniform mixing of gases, so that the isotope CO2 can fully contact the plants, and will not cause damage or interference to the plants due to excessive airflow, which helps to maintain a stable pulse labeling environment, improve the reliability and comparability of experimental results, and provide stable and reliable experimental data support for scientific research.

[0045] Preferably, the material of the incubator is polymethyl methacrylate.

[0046] The polymethyl methacrylate in the present invention has good transparency, chemical stability and mechanical strength. Transparency allows researchers to directly observe the growth status of plants and promptly discover abnormal conditions in the growth process of plants; chemical stability can prevent the incubator from chemically reacting with experimental substances to affect the experimental results, thereby ensuring the accuracy of experimental data; mechanical strength can ensure that the incubator is not easily damaged during long-term use, thereby increasing the service life of the device and providing a good foundation for long-term, multi-batch plant cultivation and 13 CO2 pulse labeling experiments provide stable and reliable container protection.

[0047] Preferably, the plant culture support has a height of 160 mm and a diameter of 110 mm.

[0048] The specific size design in the present invention provides suitable space and support for plant growth. The appropriate height and diameter can accommodate the plant roots and provide them with sufficient growth space, ensuring that the plant roots can develop normally during the cultivation process, which is beneficial to the healthy growth of the plant as a whole. 13 In the CO2 pulse labeling experiment, the isotope labeling experiment was carried out on the basis of stable growth of plants to improve the accuracy and reliability of experimental data, which provided a basis for studying the effects of plants on the growth of plants under specific culture base environments. 13 The CO2 response provides standardized conditions.

[0049] The second technical solution of the present invention: a simple and adjustable volume and height13 The tracking method of the CO2 pulse marking device comprises the following steps:

[0050] (S01) Selecting a marked plant and its growth cycle, analyzing the expected height of the plant, transplanting the plant into a plant culture base, and selecting a number of airtight tubes according to the expected height to assemble the device;

[0051] (S02) After the assembly is completed, all valves are closed, and an external air pump is connected through the airtight three-way valve of the airtight upper cover, and gas is slowly input through the air pump, and the value change of the membrane pressure gauge is observed to check the airtightness of the device. After the inspection is completed, the internal pressure of the device is slowly balanced to atmospheric pressure;

[0052] (S03) connecting the airtight three-way valve and the third straight-through airtight valve to an externally arranged far-infrared CO2 measuring instrument to construct a real-time monitoring channel for CO2 concentration;

[0053] (S04) using the preparation structure and the circulation structure to provide the gas and liquid required for plant growth, thereby simulating the plant growth environment, and analyzing the flow rate, density, and composition uniformity of the gas and liquid according to the attenuation and scattering of the laser through the laser transmitter and the laser receiver, and then adjusting the speed and flow rate of the environmental simulation circulation machine to ensure the stability and uniformity of the plant growth environment and meet the environmental requirements of the plants at different growth stages;

[0054] (S05) installing a temperature sensor and a humidity sensor inside the incubator, wherein the temperature sensor is installed 5 cm above the simulated soil plate, and the humidity sensor is installed 3 cm above the plant culture base, and recording the temperature and humidity data every 2 hours;

[0055] (S06) measuring the height of the plant with a ruler every 12 hours, reading and recording the plant height value, counting the number of leaves of the plant, and recording the newly grown leaves in a timely manner, and adjusting the volume of the incubator through the volume control structure according to the actual growth of the plant;

[0056] (S07) Cultivating the plant to a predetermined growth cycle, starting the air delivery pump, and sending the plant culture medium base upward into the pulse marking structure, and then observing the air pressure inside the pulse marking structure through a membrane pressure gauge, and adjusting the air pressure using an airtight three-way valve;

[0057] (S08) charging isotope CO2 according to a predetermined calculated amount based on the combination and volume of the pulse labeling structure to make the concentration reach the atmospheric level;

[0058] (S09) The plants are continuously cultivated and observed according to a predetermined cycle. After the cultivation is completed, the pulse labeling structure is disassembled, and plant and soil samples are collected separately for subsequent experimental analysis.

[0059] The present invention realizes plant transplantation from preparation before plant transplantation to environmental monitoring and regulation during the experiment, to pulse labeling operation and final sample collection. 13 Full tracking and precise control of CO2 pulse labeling experiments. The present invention can adapt to different plant height requirements by rationally selecting airtight tubes and assembling devices; the airtightness inspection step ensures the sealing of the experimental environment and reduces the source of error; constructs a CO2 concentration monitoring pathway, uses preparation and circulation structures and combines laser detection to control the environment, which can accurately simulate the plant growth environment and optimize it in real time; the monitoring of temperature, humidity, plant height and leaf number is coordinated with the volume control structure to meet the needs of plants at different growth stages and record growth data; the air pressure adjustment and isotope CO2 charging operations during the pulse labeling stage are precise and standardized; the final sample collection is used for subsequent analysis, which is helpful for in-depth research on plants in 13 The physiological changes, substance absorption and transformation during CO2 pulse labeling provide comprehensive and accurate data support and research basis for related scientific research.

[0060] The present invention has the following beneficial effects:

[0061] (1) Realize integrated and precise operation from plant cultivation to pulse labeling. The coordinated cooperation of various structures can accurately adjust the gas, liquid supply and environmental parameters, such as using preparation, circulation, detection structures and airtight valves, to provide precise and stable conditions for plant growth and labeling experiments, effectively reduce errors, improve the accuracy and reliability of experimental data, and facilitate in-depth research on plants. 13 CO2 labeling related physiological processes.

[0062] (2) The circulation structure combined with relevant detection methods can effectively simulate the material circulation in the natural environment and precisely regulate it. The volume control structure can flexibly adapt to the space according to the growth stage of the plant, and the exhaust structure stabilizes the gas environment. Together, they create a stable and controllable environment suitable for plant growth, adapting to the growth needs and experimental requirements of different plants, and facilitating the acquisition of effective experimental data under multiple stages and conditions.

[0063] (3) The pulse labeling structure ensures the sealing, safety and uniformity of the labeling space through good sealing design, reasonable component size planning and related auxiliary components. It can be flexibly adjusted according to the height of the plant to ensure accurate and efficient isotope CO2 labeling, improve the diversity and representativeness of experimental data, and provide high-quality conditions for studying the response of plants to isotope labeling.

[0064] (4) The use of incubators made of specific materials and rationally designed plant culture bases, as well as the installation of support plates at key connection points, enhance the overall stability and durability of the device, reduce experimental interference and data deviation caused by the device itself, ensure the long-term stable operation of the experiment, lay a solid foundation for long-term, multi-batch experiments, and strongly support the continued in-depth development of related scientific research. BRIEF DESCRIPTION OF THE DRAWINGS

[0065] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0066] Figure 2 It is a rear view schematic diagram of the overall structure of the present invention;

[0067] Figure 3 is a schematic diagram of an incubator of the present invention;

[0068] Figure 4 is a schematic diagram of a plant culture base of the present invention;

[0069] Figure 5 It is a schematic diagram of the preparation structure of the present invention;

[0070] Figure 6 This is a schematic diagram of the overall structure disassembly of the present invention.

[0071] Figure 7 It is a schematic diagram of the detection structure of the present invention;

[0072] Figure 8 It is a schematic diagram of the rising structure of the present invention;

[0073] Fig. 9 It is a schematic diagram of the disassembly of the ascending structure of the present invention;

[0074] Fig.10 is a schematic diagram of a bent partition of the present invention;

[0075] Fig.11 It is a schematic diagram of the volume control structure of the present invention;

[0076] Fig.12 It is a schematic diagram of the exhaust structure of the present invention;

[0077] Fig.13 It is a disassembled schematic diagram of the exhaust structure of the present invention;

[0078] Fig.14 is a schematic diagram of a vertical plate of the present invention;

[0079] Fig.15 is a schematic diagram of a second circular hole of the present invention;

[0080] Fig.16 is a schematic diagram of the bottom sealing seat of the present invention;

[0081] Fig.17 is a schematic diagram of the pulse labeling structure of the present invention;

[0082] Fig.18 is a schematic diagram of a first sealing gasket of the present invention;

[0083] Fig.19 is a schematic diagram of a first airtight tube of the present invention;

[0084] Fig. 20 is a schematic diagram of a second card slot of the present invention;

[0085] Fig.21 is a schematic diagram of a third flange of the present invention;

[0086] Fig. 22 is a schematic diagram of a second sealing gasket of the present invention;

[0087] Fig.23 is a schematic diagram of a fourth card slot of the present invention;

[0088] Fig.24 is a schematic diagram of a fifth flange of the present invention;

[0089] Fig.25 is a schematic diagram of a third sealing gasket of the present invention;

[0090] Fig.26 is a schematic diagram of the sixth card slot of the present invention;

[0091] Fig. 27 is a schematic diagram of the seventh card slot of the present invention;

[0092] Fig.28 is a schematic diagram of a fourth sealing gasket of the present invention;

[0093] Fig.29 It is a schematic diagram of an eighth flange of the present invention.

[0094] The marks in the attached drawings are: 100, incubator; 200, preparation structure; 300, pulse marking structure; 400, circulation structure; 500, detection structure; 600, rising structure; 700, volume control structure; 800, exhaust structure; 900, plant culture base; 101, storage tank; 102, simulated soil plate; 1021, culture basket; 1022, light shielding cover; 103, delivery pipe; 104, bent partition; 1041, square hole; 1001, second round hole; 201, first conical flask; 202, second conical flask; 2021, side pipe; 2022, two-way valve; 203, guide pipe; 204, diverter valve; 205, control pipe; 206, solenoid valve; 401, control valve; 4011, first circulation Circular tube; 4012, second circulation tube; 402, environmental simulation circulation machine; 4021, feeding box; 4022, discharging box; 4023, third circulation tube; 4024, fourth circulation tube; 501, laser transmitter; 502, laser receiver; 601, ventilation ring; 6011, ventilation groove; 602, air pipe; 603, air pump; 604, first rising ring; 605, second rising ring; 701, reference plate; 702, first push plate; 7021, first sliding plate; 7022, spring; 7023, first slide rail; 703, second push plate; 7031, second sliding plate; 7032, first transmission column; 7033, first servo electric cylinder; 7034, second slide rail; 704, third push plate; 70 41. third sliding plate; 7042. third transmission column; 7043. second servo electric cylinder; 7044. third slide rail; 801. rotating plate; 8011. rotating column; 8012. servo motor; 8013. fixed plate; 8014. transmission plate; 802. vertical plate; 803. first sealing plate; 8031. first triangular plate; 804. second sealing plate; 8041. second triangular plate; 805. third sealing plate; 8051. third triangular plate; 301. bottom sealing seat; 3011. first flange; 3012. first card slot; 3013. first sealing gasket; 3014. first circular hole; 302. first airtight tube; 3021. second flange; 30211. second card slot; 3022. third method flange; 30221, third slot; 3023, second sealing gasket; 303, second airtight tube; 3031, fourth flange; 30311, fourth slot; 3032, fifth flange; 30321, fifth slot; 3033, third sealing gasket; 3034, first straight-through airtight valve; 304, third airtight tube; 3041, sixth flange; 30411, sixth slot; 3042, seventh flange; 30421, seventh slot; 3043, fourth sealing gasket; 3044, second straight-through airtight valve; 305, airtight upper cover; 3051, eighth flange; 30511, eighth slot; 3052, third straight-through airtight valve; 3053, membrane pressure gauge; 3054, aviation airtight power plug;3055, airtight three-way valve; 3056, fan. ; DETAILED DESCRIPTION

[0095] The present invention is further described below in conjunction with the accompanying drawings and embodiments, but they are not intended to limit the present invention.

[0096] Easy to adjust volume and height 13 CO2 pulse labeling devices, including Figure 1 The incubator 100 shown in FIG. 1 has a side provided with a Figure 2 The preparation structure 200 shown, a pulse marking structure 300 is arranged above the incubator 100, a detection structure 500 is arranged on a side adjacent to the preparation structure 200, a circulation structure 400 is arranged on a side of the detection structure 500 away from the preparation structure 200, a rising structure 600 is arranged at the bottom of the incubator 100, a plant culture medium seat 900 is arranged above the rising structure 600, a volume control structure 700 is arranged above the plant culture medium seat 900, and an exhaust structure 800 is arranged on a side of the incubator 100 away from the detection structure 500;

[0097] The bottom edge of the incubator 100 is provided with Figure 3 The storage tank 101 shown in the figure has a simulated soil plate 102 disposed inside the storage tank 101, and a culture basket 1021 disposed inside the simulated soil plate 102;

[0098] A light shielding cover 1022 is detachably connected to the top of the culture basket 1021;

[0099] A bent partition 104 is detachably connected to the upper side of the light shielding cover 1022;

[0100] One side of the simulated soil plate 102 is provided with Figure 4 The connecting pipe 1011 shown, one end of the connecting pipe 1011 is connected to the storage tank 101, and the end of the connecting pipe 1011 away from the storage tank 101 is connected to the culture basket 1021;

[0101] A delivery pipe 103 is provided on a side of the incubator 100 away from the connecting pipe 1011 .

[0102] like Figure 5The preparation structure 200 shown includes a two-way valve 2022, which is connected to the end of the delivery tube 103 away from the incubator 100, and the end of the two-way valve 2022 away from the delivery tube 103 is detachably connected to a side tube 2021, and the end of the side tube 2021 away from the two-way valve 2022 is fixedly connected to a first conical flask 201, and the top of the first conical flask 201 is detachably connected to a guide tube 203, and the end of the guide tube 203 away from the first conical flask 201 is provided with a second conical flask 202, and a diverter valve 204 is provided in the middle of the guide tube 203, and a control tube 205 is provided at the top of the diverter valve 204, and an electromagnetic valve 206 is provided at the end of the control tube 205 away from the diverter valve 204.

[0103] like Figure 6 The circulation structure 400 shown includes a first circulation pipe 4011, one end of which is connected to the culture basket 1021, the first circulation pipe 4011 passes through the simulated soil plate 102 and the culture box 100, a control valve 401 is provided at one end of the first circulation pipe 4011 away from the culture basket 1021, a second circulation pipe 4012 is provided on one side of the control valve 401, a feed box 4021 is provided at one end of the second circulation pipe 4012 away from the control valve 401, an environment simulation circulation machine 402 is provided on one side of the feed box 4021 away from the second circulation pipe 4012, a discharge box 4022 is provided on one side of the bottom end of the environment simulation circulation machine 402, a third circulation pipe 4023 and a fourth circulation pipe 4024 are provided on one side of the discharge box 4022 away from the environment simulation circulation machine 402, and one end of the third circulation pipe 4023 and the fourth circulation pipe 4024 away from the discharge box 4022 is connected to the culture basket 1021.

[0104] like Figure 7 The detection structure 500 shown includes a laser transmitter 501 , which is disposed on one side of the control valve 401 . A laser receiver 502 is disposed on the side of the control valve 401 away from the laser transmitter 501 . The first circulation pipe 4011 is located between the laser transmitter 501 and the laser receiver 502 .

[0105] A plurality of ventilation holes are provided on the side of the plant culture medium base 900 .

[0106] like Figure 8 The rising structure 600 shown in the figure includes a ventilation ring 601, which is fixedly connected to the bottom center of the culture basket 1021. A gas delivery pipe 602 is arranged on the side of the ventilation ring 601. An air delivery pump 603 is arranged at one end of the gas delivery pipe 602 away from the ventilation ring 601. The inner side of the ventilation ring 601 is slidably connected to the inner side of the ventilation ring 601. Fig. 9 The first rising ring 604 shown, the inner side of which is slidably connected to the second rising ring 605;

[0107] A ventilation groove 6011 is provided at the bottom end of the ventilation ring 601 , and one end of the ventilation groove 6011 is connected to the air delivery pipe 602 .

[0108] like Fig.10 A square hole 1041 is opened at the center of the bent partition 104 , and the square hole 1041 is located directly above the plant culture base 900 .

[0109] like Fig.11 The volume control structure 700 shown includes a reference plate 701, which is disposed above the bent partition 104, and one end of the reference plate 701 is detachably connected to the incubator 100;

[0110] A first push plate 702 is provided at one end of the reference plate 701 away from the incubator 100, one end of the first push plate 702 is fixedly connected to a first sliding plate 7021, one end of the first sliding plate 7021 away from the first push plate 702 is fixedly connected to a spring 7022, one end of the spring 7022 away from the first sliding plate 7021 is fixedly connected to a first slide rail 7023, the first sliding plate 7021 is slidably connected to the first slide rail 7023, and one side of the bottom of the first slide rail 7023 is detachably connected to the incubator 100;

[0111] A second push plate 703 is provided on a side of the first push plate 702 away from the first sliding plate 7021, a second sliding plate 7031 is fixedly connected to a side of the second push plate 703 away from the first push plate 702, a first transmission column 7032 is fixedly connected to one end of the second sliding plate 7031 away from the second push plate 703, a first servo electric cylinder 7033 is provided on one end of the first transmission column 7032 away from the second sliding plate 7031, an output end of the first servo electric cylinder 7033 is fixedly connected to the first transmission column 7032, a second slide rail 7034 is provided below the first servo electric cylinder 7033, the second sliding plate 7031 is slidably connected to the second slide rail 7034, and one side of the bottom of the second slide rail 7034 is detachably connected to the incubator 100;

[0112] A third push plate 704 is provided on one side of the second push plate 703, and a third sliding plate 7041 is fixedly connected to the side of the third push plate 704 away from the second push plate 703, and a third transmission column 7042 is fixedly connected to the end of the third sliding plate 7041 away from the third push plate 704, and a second servo electric cylinder 7043 is provided on the end of the third transmission column 7042 away from the third sliding plate 7041, and an output end of the second servo electric cylinder 7043 is fixedly connected to the third transmission column 7042, and a third slide rail 7044 is provided below the second servo electric cylinder 7043, the third sliding plate 7041 is slidably connected to the third slide rail 7044, and a bottom side of the third slide rail 7044 is detachably connected to the incubator 100.

[0113] like Fig.12The exhaust structure 800 shown includes a rotating plate 801, which is arranged on the side of the bent partition 104, and a rotating column 8011 is fixedly connected to the side of the rotating plate 801 close to the incubator 100, and the side of the rotating column 8011 is rotatably connected to the incubator 100, and a servo motor 8012 is arranged at one end of the rotating column 8011 away from the rotating plate 801, and the output end of the servo motor 8012 is fixedly connected to the rotating column 8011, and a fixed plate 8013 is arranged below the servo motor 8012, and one side of the fixed plate 8013 is fixedly connected to the incubator 100;

[0114] like Fig.13 The rotating plate 801 shown is rotatably connected to a transmission plate 8014 on one side, and the transmission plate 8014 is rotatably connected to a vertical plate 802 at one end away from the rotating plate 801. The top of the vertical plate 802 is rotatably connected to a first triangular plate 8031, and the first triangular plate 8031 ​​is fixedly connected to a first sealing plate 803 at one end away from the vertical plate 802.

[0115] like Fig.14 The middle part of the vertical plate 802 is rotatably connected to the second triangular plate 8041, and one end of the second triangular plate 8041 away from the vertical plate 802 is fixedly connected to the second sealing plate 804;

[0116] The bottom end of the vertical plate 802 is rotatably connected to a third triangular plate 8051, and a third sealing plate 805 is fixedly connected to a side of the third triangular plate 8051 away from the vertical plate 802;

[0117] Both sides of the first sealed plate 803 , the second sealed plate 804 and the third sealed plate 805 are rotatably connected to the incubator 100 .

[0118] The pulse marking structure 300 includes a bottom sealing seat 301, which is arranged above the incubator 100. The bottom surface of the bottom sealing seat 301 is detachably connected to the upper surface of the incubator 100. A first airtight tube 302 is arranged above the bottom sealing seat 301, a second airtight tube 303 is arranged above the first airtight tube 302, a third airtight tube 304 is arranged above the second airtight tube 303, and an airtight upper cover 305 is arranged above the third airtight tube 304.

[0119] like Fig.16 A first circular hole 3014 is provided at the center of the bottom sealing seat 301. Fig.15 A second circular hole 1001 is provided at the center of the top of the culture box 100 shown. The centers of the first circular hole 3014, the second circular hole 1001 and the plant culture base 900 are located on the same vertical line. The sizes of the first circular hole 3014, the second circular hole 1001 and the plant culture base 900 are adapted to each other.

[0120] like Fig.17 The first airtight tube 302 shown has a length of 200 mm and an outer wall thickness of 20 mm;

[0121] The second airtight tube 303 has a length of 400 mm and an outer wall thickness of 20 mm;

[0122] The third airtight tube 304 has a length of 700 mm and an outer wall thickness of 20 mm;

[0123] The diameters of the bottom sealing seat 301, the first airtight tube 302, the second airtight tube 303 and the third airtight tube 304 are 300 mm.

[0124] like Fig.18 The bottom edge of the bottom sealing seat 301 shown is fixedly connected with a first flange 3011, a first groove 3012 is provided on the inner side of the first flange 3011, a first sealing gasket 3013 is provided above the first groove 3012, a second flange 3021 is detachably connected above the first flange 3011, the inner side of the second flange 3021 is fixedly connected with the bottom edge of the first airtight tube 302, a second groove 30211 is provided on the bottom surface of the second flange 3021, and the upper surface of the first sealing gasket 3013 is in contact with the second groove 30211;

[0125] like Fig.19 The top edge of the first airtight tube 302 is fixedly connected with a third flange 3022, as shown in FIG. Fig. 20 The third flange 3022 shown in the figure has a third slot 30221 formed inside, a second sealing gasket 3023 is disposed above the third slot 30221, a fourth flange 3031 is detachably connected to the top of the third flange 3022, and the inner side of the fourth flange 3031 is fixedly connected to the bottom edge of the second airtight tube 303, as shown in the figure. Fig.21 The bottom surface of the fourth flange 3031 is provided with a fourth slot 30311. Fig. 22 The upper surface of the second sealing gasket 3023 is fitted with the fourth slot 30311;

[0126] like Fig.23 The top edge of the second airtight tube 303 is fixedly connected with a fifth flange 3032, as shown in FIG. Fig.24 The fifth flange 3032 is provided with a fifth groove 30321 on the inner side thereof, and a third sealing gasket 3033 is provided above the fifth groove 30321. Fig.25 The sixth flange 3041 is detachably connected to the upper side of the fifth flange 3032, the inner side of the sixth flange 3041 is fixedly connected to the bottom edge of the third airtight tube 304, the bottom surface of the sixth flange 3041 is provided with a sixth groove 30411, and the upper surface of the third sealing gasket 3033 is fitted with the sixth groove 30411;

[0127] like Fig.26 The top edge of the third airtight tube 304 is fixedly connected with a seventh flange 3042, and the inner side of the seventh flange 3042 is provided with a seventh slot 30421. Fig. 27 A fourth sealing gasket 3043 is disposed above the seventh slot 30421 as shown. Fig.28 The seventh flange 3042 shown is detachably connected to the top with the eighth flange 3051, the inner side of the eighth flange 3051 is fixedly connected to the edge of the airtight upper cover 305, the bottom surface of the eighth flange 3051 is provided with an eighth groove 30511, and the upper surface of the fourth sealing gasket 3043 fits with the eighth groove 30511.

[0128] An upper support plate 306 is disposed above the second flange 3021 , the fourth flange 3031 and the sixth flange 3041 , and a lower support plate 307 is disposed below the third flange 3022 , the fifth flange 3032 and the seventh flange 3042 .

[0129] A first straight airtight valve 3034 is provided on the side of the second airtight tube 303;

[0130] A second direct airtight valve 3044 is provided on the side of the third airtight tube 304;

[0131] A third direct airtight valve 3052 is provided on the upper surface edge of the airtight upper cover 305 .

[0132] like Fig.29 A membrane pressure gauge 3053 is provided at the center of the upper surface of the airtight upper cover 305 shown;

[0133] An aviation airtight power plug 3054 is provided on one side of the membrane pressure gauge 3053, and the aviation airtight power plug 3054 is electrically connected to the airtight upper cover 305;

[0134] An airtight three-way valve 3055 is provided on the side of the aviation airtight power plug 3054 away from the membrane pressure gauge 3053;

[0135] A fan 3056 is provided on the bottom surface of the airtight upper cover 305 .

[0136] The diameter of fan 3056 is 100 mm.

[0137] The material of the incubator 100 is polymethyl methacrylate.

[0138] The height of the plant culture base 900 is 160 mm and the diameter is 110 mm.

[0139] Easy to adjust volume and height 13 The tracking method of the CO2 pulse marking device comprises the following steps:

[0140] (S01) Select the marked plant and its growth cycle, analyze the expected height of the plant, transplant the plant into the plant culture base 900, select a number of airtight tubes according to the expected height, and assemble the device;

[0141] (S02) After the assembly is completed, all valves are closed, and an external air pump is connected through the airtight three-way valve 3055 on the airtight upper cover 305, and gas is slowly input through the air pump, and the value change of the membrane pressure gauge 3053 is observed to check the airtightness of the device. After the inspection is completed, the internal pressure of the device is slowly balanced to atmospheric pressure;

[0142] (S03) Connecting the airtight three-way valve 3055 and the third direct airtight valve 3052 to an externally arranged far-infrared CO2 measuring instrument to construct a real-time monitoring channel for CO2 concentration;

[0143] (S04) Using the preparation structure 200 and the circulation structure 400 to provide the gas and liquid required for plant growth, thereby simulating the plant growth environment, and analyzing the flow rate, density, and composition uniformity of the gas and liquid according to the attenuation and scattering of the laser through the laser transmitter 501 and the laser receiver 502, and then adjusting the speed and flow rate of the environment simulation circulation machine 402 to ensure the stability and uniformity of the plant growth environment and meet the environmental requirements of the plants at different growth stages;

[0144] (S05) Install a temperature sensor and a humidity sensor inside the incubator 100, the temperature sensor is installed 5 cm above the simulated soil plate 102, and the humidity sensor is installed 3 cm above the plant culture base 900, and record the temperature and humidity data every 2 hours;

[0145] (S06) Using a ruler to measure the height of the plant every 12 hours, reading and recording the plant height value, counting the number of leaves of the plant, and recording the newly grown leaves in a timely manner, and adjusting the volume of the incubator 100 through the volume control structure 700 according to the actual growth of the plant;

[0146] (S07) Cultivate the plant to a predetermined growth cycle, start the air delivery pump 603, and send the plant culture medium base 900 upward into the pulse marking structure 300, then observe the air pressure inside the pulse marking structure 300 through the membrane pressure gauge 3053, and adjust the air pressure using the airtight three-way valve 3055;

[0147] (S08) charging isotope CO2 according to the combination and volume of the pulse labeling structure 300 according to a predetermined calculated amount to make the concentration reach the atmospheric level;

[0148] (S09) The plants are continuously cultivated and observed according to a predetermined cycle. After the cultivation is completed, the pulse labeling structure 300 is disassembled, and plant and soil samples are collected respectively for subsequent experimental analysis.

[0149] Experimental Example 1:

[0150] This experimental example takes air tightness detection as an example.

[0151] according to Figure 1 Easy assembly volume and height adjustment shown 13 CO pulse labeling device. First, the incubator 100, the preparation structure 200, the pulse labeling structure 300, the detection structure 500, the circulation structure 400, the rising structure 600, the plant culture base 900, the volume control structure 700 and the exhaust structure 800 are connected and fixed.

[0152] After the device is assembled, 500 mL of 5% carbon dioxide is filled into the device through the preparation structure 200. The specific operation is to connect the relevant gas source to the first conical flask 201, and use the two-way valve 2022 to control the gas flow direction so that the gas enters the incubator 100 through the flow guide tube 203. Then, the fan 3056 on the bottom of the airtight upper cover 305 in the pulse marking structure 300 is turned on to fully mix the gas in the device.

[0153] During the experiment, 20 mL of gas was collected from the pulse marking structure 300 every 8 hours using a gas-tight syringe. During the collection, the gas-tight syringe was connected to the gas-tight three-way valve 3055, and the gas sample was extracted under the premise of ensuring gas tightness. Then, the CO2 concentration of the collected gas sample was measured using a gas chromatograph.

[0154] After 72 hours of continuous observation, the results showed that the CO2 concentration in the tank did not drop significantly. This result fully demonstrates that the airtightness of this embodiment is good, and the connection parts and sealing components effectively prevent gas leakage. At the same time, it also reflects that the device has good pressure bearing capacity. During the gas filling, mixing and long-term observation process, the device structure is stable, and there is no deformation or sealing failure due to changes in internal air pressure, which strongly proves that this embodiment is 13 The reliability and stability in CO2 pulse labeling experimental applications can provide a precise and stable experimental environment for related plant physiological research.

[0155] Experimental Example 2

[0156] This experiment takes the rice marking experiment in the grain filling stage as an example.

[0157] This example adopts the patented simple and adjustable volume and height 13 CO2 pulse labeling device was used to label rice during the grain filling period 13The CO2 pulse labeling experiment aims to reveal the distribution of photosynthetic products in rice rhizosphere microorganisms. The rice variety selected for the experiment is "Jia 67".

[0158] First, a rice seedling that had been cultivated for 15 days was carefully transferred to a plant culture base 900 containing 1.5 kg of dry soil. Then, a carefully prepared fertilizer solution was added to the plant culture base 900, and then flooding was performed. The amount of fertilizer used in this experiment was set to 100 mgN·kg -1 , 20mgP·kg -1 、80mgK·kg -1 , and the nitrogen, phosphorus and potash fertilizers applied are ammonium sulfate, superphosphate and potassium chloride respectively. The rice seedlings are then placed in the incubator 100 for continuous cultivation until the rice enters the grain filling stage, a 2 cm flooding layer is always maintained during the entire cultivation period, and Hogland nutrient solution is added in time according to the growth trend of the rice plants to ensure the healthy growth of the rice.

[0159] When the rice enters the grain filling stage, the rising structure 600 is used to send 1-3 pots of rice in the grain filling stage into the pulse marking structure 300. Then, the first direct airtight valve 3034 on the side wall of the second airtight tube 303 is connected to an external water pump through a silicone tube, so that water can be replenished in time during the cultivation period, thereby effectively maintaining the flooding state.

[0160] After the assembly is completed, close all valves of the pulse marking structure 300, slowly fill the tank with air through the airtight three-way valve 3055 to keep the tank in a positive pressure state, and accurately judge the airtightness of the device based on the reading of the membrane pressure gauge 3053. If leakage is found during the test, carefully adjust the fastening bolts between the components until the device is airtight. After confirming that the airtightness of the device meets the experimental requirements, set the lighting parameters of the laboratory where the device is located, with a lighting time of 14 hours and a dark time of 10 hours.

[0161] Finally, during the period from 8:00 a.m. to 4:00 p.m. every day, a gas-tight syringe is used to accurately fill the specified volume of high-concentration CO2 every half an hour based on the real-time readings of the external far-infrared CO2 measuring instrument. 13 CO2 is used to ensure that the CO2 concentration in the device can be stably maintained at 400ppm. After 12 consecutive days of labeling, the device is carefully disassembled, and plant and soil samples are carefully collected and properly preserved for subsequent in-depth analysis and research, thereby providing reliable experimental samples and data support for a comprehensive and in-depth study of the distribution of rice photosynthetic products in rhizosphere microorganisms.

[0162] Experimental Example 3

[0163] In this experiment, we prepared high-abundance 13C marks corn stalks as an example.

[0164] In this example, in order to meet the needs of research related to straw degradation, the corn variety for testing was determined to be "Xianyu 335".

[0165] First, plump corn kernels were selected, sterilized with 10% (v / v) hydrogen peroxide for 10 minutes, and then repeatedly rinsed with sterile deionized water, and then placed in a moist sterile culture dish, moved into an incubator 100, and germinated in a dark environment at a temperature of 25°C. After the seeds germinated, they were transferred to a plant culture medium base 900 containing 1L of sterilized vermiculite, and about 300mL of 0.5 times Hollander's nutrient solution was poured into it to make the water content of the substrate reach 65%. A round PVC water tray with a diameter of 15cm and a height of 5cm was added to the bottom of the plant culture medium base 900.

[0166] When the corn seedlings grow to the 2-leaf stage, the device at this time consists of a bottom sealing seat 301, a transparent airtight upper cover 305 and a transparent second airtight tube 303. The first straight-through airtight valve 3034 arranged on the side of the second airtight tube 303 is connected to an external water pump through a silicone tube, and a digital soil moisture meter is inserted into the plant culture medium seat 900 to observe the moisture content in real time, so that water can be replenished in time.

[0167] Use an external air pump to fill the pulse marking structure 300 with air to keep the pulse marking structure 300 in a positive pressure state for 24 hours to check the air tightness of the pulse marking structure 300. If leakage is found during the inspection, carefully adjust the fastening bolts between the components until the device is airtight. After confirming that the air tightness of the device meets the requirements, set the light and temperature parameters of the laboratory where the device is located, including 16 hours of light, 8 hours of darkness, 25°C during the day, and 18°C ​​at night.

[0168] Then, the CO2 concentration in the device was continuously monitored by an external far-infrared CO2 measuring instrument, and high-purity CO2 was injected into the device through an airtight three-way valve 3055 using an airtight syringe every 1 hour. 13 CO2 was added to maintain the CO2 concentration in the device at around 400 ppm. In addition, 50 mL of 1× Hoagland nutrient solution was injected every 2 days to ensure the nutrient supply of the corn seedlings.

[0169] When the seedlings grow to the top of the device, open the airtight cover 305 in the dark, add the first airtight tube 302, and pour out the water in the water tray. Then check the airtightness of the device again according to the above steps, and continue to cultivate after confirming that it is correct.

[0170] After 2 months of cultivation, the device was carefully disassembled, the corn plants were collected, dried at 80°C and ground for use in subsequent related experiments, providing high abundance for straw degradation research. 13 C corn straw samples help to further explore scientific issues related to straw degradation.

[0171] The present invention has been described in detail above by general description and specific embodiments, but it is obvious to those skilled in the art that some modifications or improvements can be made on the basis of the present invention. Therefore, these modifications or improvements made on the basis of not departing from the spirit of the present invention all belong to the scope of protection claimed by the present invention.

Claims

1. Easy to adjust volume and height 13 The CO2 pulse marking device is characterized by: The invention comprises a culture box (100), wherein a preparation structure (200) is arranged on one side of the culture box (100), a pulse marking structure (300) is arranged above the culture box (100), a detection structure (500) is arranged on a side adjacent to the preparation structure (200), a circulation structure (400) is arranged on a side of the detection structure (500) away from the preparation structure (200), a rising structure (600) is arranged at the bottom of the culture box (100), a plant culture base (900) is arranged above the rising structure (600), a volume control structure (700) is arranged above the plant culture base (900), and an exhaust structure (800) is arranged on a side of the culture box (100) away from the detection structure (500); The bottom edge of the culture box (100) is provided with a storage groove (101), the inner side of the storage groove (101) is provided with a simulated soil plate (102), and the inner side of the simulated soil plate (102) is provided with a culture basket (1021); A light shielding cover (1022) is detachably connected to the top of the culture basket (1021); A bent partition plate (104) is detachably connected to the upper side of the light shielding cover (1022); A connecting pipe (1011) is provided on one side of the simulated soil plate (102), one end of the connecting pipe (1011) is connected to the storage tank (101), and one end of the connecting pipe (1011) away from the storage tank (101) is connected to the culture basket (1021); A delivery pipe (103) is provided on a side of the incubator (100) away from the connecting pipe (1011).

2. The volume and height adjustable simple device according to claim 1 13 The CO2 pulse marking device is characterized by: The preparation structure (200) comprises a two-way valve (2022), wherein the two-way valve (2022) is connected to one end of the delivery tube (103) away from the incubator (100), the end of the two-way valve (2022) away from the delivery tube (103) is detachably connected to a side tube (2021), the end of the side tube (2021) away from the two-way valve (2022) is fixedly connected to a first conical flask (201), the top end of the first conical flask (201) is detachably connected to a flow guide tube (203), the end of the flow guide tube (203) away from the first conical flask (201) is provided with a second conical flask (202), a diverter valve (204) is provided in the middle of the diverter valve (203), the top end of the diverter valve (204) is provided with a control tube (205), and the end of the control tube (205) away from the diverter valve (204) is provided with an electromagnetic valve (206).

3. The volume and height adjustable simple device according to claim 1 13 The CO2 pulse marking device is characterized by: The circulation structure (400) comprises a first circulation pipe (4011), one end of the first circulation pipe (4011) is connected to the culture basket (1021), the first circulation pipe (4011) passes through the simulated soil plate (102) and the culture box (100), a control valve (401) is provided at one end of the first circulation pipe (4011) away from the culture basket (1021), a second circulation pipe (4012) is provided on one side of the control valve (401), and a feed box (4012) is provided at one end of the second circulation pipe (4012) away from the control valve (401). 4021), an environmental simulation circulation machine (402) is arranged on the side of the feed box (4021) away from the second circulation pipe (4012), a discharge box (4022) is arranged on the bottom side of the environmental simulation circulation machine (402), a third circulation pipe (4023) and a fourth circulation pipe (4024) are arranged on the side of the discharge box (4022) away from the environmental simulation circulation machine (402), and one end of the third circulation pipe (4023) and the fourth circulation pipe (4024) away from the discharge box (4022) is connected to the culture basket (1021).

4. The volume and height adjustable simple device according to claim 3 13 The CO2 pulse marking device is characterized by: The detection structure (500) comprises a laser transmitter (501), wherein the laser transmitter (501) is arranged on one side of a control valve (401), a laser receiver (502) is arranged on the side of the control valve (401) away from the laser transmitter (501), and the first circulation pipe (4011) is located between the laser transmitter (501) and the laser receiver (502).

5. The volume and height adjustable simple device according to claim 1 13 The CO2 pulse marking device is characterized by: The ascending structure (600) comprises a ventilation ring (601), the ventilation ring (601) is fixedly connected to the bottom center of the culture basket (1021), an air supply pipe (602) is arranged on the side of the ventilation ring (601), an air supply pump (603) is arranged at one end of the air supply pipe (602) away from the ventilation ring (601), a first ascending ring (604) is slidably connected to the inner side of the ventilation ring (601), and a second ascending ring (605) is slidably connected to the inner side of the first ascending ring (604); A ventilation groove (6011) is provided at the bottom end of the ventilation ring (601), and one end of the ventilation groove (6011) is connected to the air delivery pipe (602).

6. The volume and height adjustable simple device according to claim 1 13 The CO2 pulse marking device is characterized by: The volume control structure (700) comprises a reference plate (701), wherein the reference plate (701) is arranged above the bent partition plate (104), and one end of the reference plate (701) is detachably connected to the incubator (100); A first push plate (702) is provided at one end of the reference plate (701) away from the incubator (100); one end of the first push plate (702) is fixedly connected to a first sliding plate (7021); one end of the first sliding plate (7021) away from the first push plate (702) is fixedly connected to a spring (7022); one end of the spring (7022) away from the first sliding plate (7021) is fixedly connected to a first slide rail (7023); the first sliding plate (7021) is slidably connected to the first slide rail (7023); and one side of the bottom of the first slide rail (7023) is detachably connected to the incubator (100); A second push plate (703) is provided on a side of the first push plate (702) away from the first sliding plate (7021); a second sliding plate (7031) is fixedly connected to a side of the second push plate (703) away from the first push plate (702); a first transmission column (7032) is fixedly connected to one end of the second sliding plate (7031) away from the second push plate (703); a first servo electric cylinder (7033) is provided on one end of the first transmission column (7032) away from the second sliding plate (7031); an output end of the first servo electric cylinder (7033) is fixedly connected to the first transmission column (7032); a second slide rail (7034) is provided below the first servo electric cylinder (7033); the second slide plate (7031) is slidably connected to the second slide rail (7034); and a bottom side of the second slide rail (7034) is detachably connected to the incubator (100); A third push plate (704) is provided on one side of the second push plate (703); a third sliding plate (7041) is fixedly connected to a side of the third push plate (704) away from the second push plate (703); a third transmission column (7042) is fixedly connected to one end of the third sliding plate (7041) away from the third push plate (704); a second servo electric cylinder (7043) is provided on one end of the third transmission column (7042) away from the third sliding plate (7041); an output end of the second servo electric cylinder (7043) is fixedly connected to the third transmission column (7042); a third slide rail (7044) is provided below the second servo electric cylinder (7043); the third sliding plate (7041) is slidably connected to the third slide rail (7044); and a bottom side of the third slide rail (7044) is detachably connected to the incubator (100).

7. The volume and height adjustable simple device according to claim 1 13 The CO2 pulse marking device is characterized by: The exhaust structure (800) comprises a rotating plate (801), the rotating plate (801) being arranged on the side of the bent partition plate (104), a rotating column (8011) being fixedly connected to a side of the rotating plate (801) close to the incubator (100), a side of the rotating column (8011) being rotatably connected to the incubator (100), a servo motor (8012) being arranged at one end of the rotating column (8011) away from the rotating plate (801), an output end of the servo motor (8012) being fixedly connected to the rotating column (8011), a fixed plate (8013) being arranged below the servo motor (8012), and one side of the fixed plate (8013) being fixedly connected to the incubator (100); One side of the rotating plate (801) is rotatably connected to a transmission plate (8014), one end of the transmission plate (8014) away from the rotating plate (801) is rotatably connected to a vertical plate (802), the top of the vertical plate (802) is rotatably connected to a first triangular plate (8031), and one end of the first triangular plate (8031) away from the vertical plate (802) is fixedly connected to a first sealing plate (803); The middle part of the vertical plate (802) is rotatably connected to a second triangular plate (8041), and one end of the second triangular plate (8041) away from the vertical plate (802) is fixedly connected to a second sealing plate (804); The bottom end of the vertical plate (802) is rotatably connected to a third triangular plate (8051), and a side of the third triangular plate (8051) away from the vertical plate (802) is fixedly connected to a third sealing plate (805); Both sides of the first sealed plate (803), the second sealed plate (804) and the third sealed plate (805) are rotatably connected to the incubator (100).

8. The volume and height adjustable simple device according to claim 1 13 The CO2 pulse marking device is characterized by: The pulse marking structure (300) includes a bottom sealing seat (301), which is arranged above the incubator (100), and the bottom surface of the bottom sealing seat (301) is detachably connected to the upper surface of the incubator (100), a first airtight tube (302) is arranged above the bottom sealing seat (301), a second airtight tube (303) is arranged above the first airtight tube (302), a third airtight tube (304) is arranged above the second airtight tube (303), and an airtight upper cover (305) is arranged above the third airtight tube (304).

9. The volume and height adjustable simple device according to claim 8 13 The CO2 pulse marking device is characterized by: A first circular hole (3014) is provided at the center of the bottom sealing seat (301), and a second circular hole (1001) is provided at the center of the top of the culture box (100). The centers of the first circular hole (3014), the second circular hole (1001) and the plant culture base (900) are located on the same vertical line, and the sizes of the first circular hole (3014), the second circular hole (1001) and the plant culture base (900) are adapted to each other.

10. Easy to adjust volume and height 13 The CO2 pulse labeling method is characterized by: Includes the following step, (S01) Selecting a marked plant and its growth cycle, analyzing the expected height of the plant, transplanting the plant into a plant culture base (900), and selecting a number of airtight tubes according to the expected height to assemble the device; (S02) After the assembly is completed, all valves are closed, and an external air pump is connected through the airtight three-way valve (3055) on the airtight upper cover (305). Gas is slowly input through the air pump, and the value change of the membrane pressure gauge (3053) is observed to check the airtightness of the device. After the inspection is completed, the internal pressure of the device is slowly balanced to atmospheric pressure; (S03) connecting the airtight three-way valve (3055) and the third direct airtight valve (3052) to an externally arranged far-infrared CO2 measuring instrument to construct a real-time monitoring channel for CO2 concentration; (S04) using the preparation structure (200) and the circulation structure (400) to provide gas and liquid required for plant growth, thereby simulating the plant growth environment, and analyzing the flow rate, density, and composition uniformity of the gas and liquid according to the attenuation and scattering of the laser through the laser transmitter (501) and the laser receiver (502), and then adjusting the rotation speed and flow rate of the environment simulation circulation machine (402) to ensure the stability and uniformity of the plant growth environment and meet the environmental requirements of the plants at different growth stages; (S05) installing a temperature sensor and a humidity sensor inside the incubator (100), wherein the temperature sensor is installed 5 cm above the simulated soil plate (102), and the humidity sensor is installed 3 cm above the plant culture base (900), and recording the temperature and humidity data every 2 hours; (S06) measuring the height of the plant with a ruler every 12 hours, reading and recording the plant height value, counting the number of leaves of the plant, and recording the newly grown leaves in a timely manner, and adjusting the volume of the incubator (100) through the volume control structure (700) according to the actual growth of the plant; (S07) Cultivating the plant to a predetermined growth cycle, starting the air delivery pump (603), sending the plant culture medium base (900) upward into the pulse marking structure (300), and then observing the air pressure inside the pulse marking structure (300) through the membrane pressure gauge (3053), and adjusting the air pressure using the airtight three-way valve (3055); (S08) charging isotope CO2 according to the combination and volume of the pulse labeling structure (300) according to a predetermined calculated amount to make the concentration reach the atmospheric level; (S09) The plants are continuously cultivated and observed according to a predetermined cycle. After the cultivation is completed, the pulse labeling structure (300) is disassembled, and plant and soil samples are collected respectively for subsequent experimental analysis.

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