Dynamic detection method and device for platelet detection and computer readable storage medium

Abstract

The invention relates to the technical field of in-vitro diagnosis, and discloses a dynamic detection method and device for platelet detection and a computer readable storage medium. A first detection mode and a second detection mode can be started; the first to-be-tested sample is supplied to a detection channel according to a preset first statistic # imgabs0 #, and the number of platelet particles # imgabs1 # is obtained through statistics; calculating to obtain a third statistic # imgabs2 # of the required first to-be-tested sample; and continuously supplying the third statistic # imgabs3 # into the detection channel for detection, and rapidly obtaining a first platelet detection result. In this way, it can be guaranteed that the sufficient number of platelet ions can be counted, and the detection precision is improved.

Description

Technical Field

[0001] The present invention belongs to the field of in vitro diagnostic technology, specifically a dynamic detection method, device and computer-readable storage medium for platelet detection. This application is a divisional application of patent application 2024112756297 Dynamic detection method, device and computer-readable storage medium for platelet detection filed on September 12, 2024. Background Art

[0002] Platelets (PLT) have important clinical detection reference value, and there are high requirements for the accuracy of their detection. If a low-value platelet sample is detected, the accuracy of the platelet may be affected due to the small number of platelet statistics. Therefore, for low-value platelet samples, it is necessary to consider increasing their statistics to improve the accuracy of platelet detection.

[0003] In some existing PLT detection technologies, the impedance method is first used to detect platelets to obtain the test results of PLT_I. If the sample is judged to be a low-value platelet sample based on the PLT_I test results, the test solution is re-prepared or the remaining test solution is reused, and a test solution with more statistics is provided to the impedance channel or RET channel. At present, this technology needs to determine whether the sample is a low-value platelet sample in the impedance channel, and then re-prepare the test solution or reuse the remaining test solution. On the one hand, re-preparing the test solution will increase the time for re-preparing the test solution and increase the reagent consumption; even if the test solution is not re-prepared, and only the remaining test solution is used, the amount of test solution prepared at the beginning will be much more than the amount of a single test, in order to ensure that there is enough remaining test solution and provide a test solution with more statistics. On the other hand, if the impedance channel analysis is abnormal, such as pore blockage, it is impossible to determine whether it needs to be multiplied. There may be a RET channel that needs to increase the statistics for re-testing, which will increase the reagent consumption of the RET channel and affect the sample detection efficiency. At the same time, in the existing PLT detection method, it is only determined whether the sample is a low-platelet sample, and the statistics of the test solution of the sample determined to be a low-platelet sample are uniformly increased to a predetermined value. There may be a situation where the increased statistics still cannot accurately detect the platelets in the sample. Summary of the invention

[0004] In view of this, the object of the present invention is to provide a dynamic and efficient platelet detection method, device and computer-readable storage medium, which are conducive to improving the detection accuracy of platelet samples and maximizing the detection efficiency.

[0005] In order to achieve the above object, the present invention provides the following technical solutions: A dynamic and efficient platelet detection method, the method comprising: Enable the first detection mode or the second detection mode; When the first detection mode is enabled: A first sample to be tested is prepared using a blood sample, and the first sample to be tested is supplied to a detection channel for testing according to a preset fourth statistic, and a detection signal of the first sample to be tested is obtained, wherein the detection signal of the first sample to be tested is at least used to obtain a first platelet test result of the blood sample, and the first platelet test result is output; When the second detection mode is enabled: A second sample to be tested is prepared using a blood sample, and the second sample to be tested is supplied to the detection channel using a power device. The second sample to be tested is first supplied to the detection channel for detection according to a preset first statistic, and a detection signal of the second sample to be tested is obtained to obtain second platelet detection data, and a second statistic of the required second sample to be tested is calculated using a preset platelet statistical particle number threshold and the second platelet detection data, and the second sample to be tested is pushed into the detection channel according to the calculated second statistic, and a third platelet detection result is output; Wherein, the second platelet detection data at least includes the statistical platelet particle count when the supply amount of the second sample to be tested reaches a preset first statistical amount.

[0006] Wherein, the number of platelet particles in the second test sample of the second statistic is greater than or equal to a preset platelet statistical particle number threshold.

[0007] Among them, the preset platelet particle count threshold is a set platelet particle count lower limit. When it is lower than the lower limit, the number of platelet particles counted in the detection channel is insufficient, and the obtained detection signal is also insufficient, and there is a situation where the accuracy of the platelet detection result is not high. Therefore, it is necessary to ensure that the number of platelet particles counted in the detection channel is not lower than the set platelet particle count lower limit.

[0008] In the second detection mode, by pre-acquiring the detection signal of the second test sample of the first statistic and obtaining the second platelet detection data, and then using the preset platelet statistical particle number threshold and the second platelet detection data to calculate the second statistic of the required second test sample, it can be ensured that the total number of PLT particles counted is always not less than the preset platelet statistical particle number threshold, and sufficient detection signals are obtained, and ultimately the detection precision and accuracy of the platelet detection results are ensured. At the same time, the judgment and identification steps of low-value platelet samples are omitted, the program flow is simplified, and the detection efficiency is improved. For some high-value samples, the second statistic calculated using the preset platelet statistical particle number threshold and the corresponding detection data may be lower than the fourth statistic set in the traditional method, and the platelet detection of the current sample is completed in advance, thereby improving the overall detection efficiency of the instrument.

[0009] Further, the detection channel is an impedance detection channel or an optical detection channel. Further, the detection channel of the first detection mode is an impedance detection channel, and the detection channel of the second detection mode is also an impedance detection channel; or, the detection channel of the first detection mode is an impedance detection channel, and the detection channel of the second detection mode is an optical detection channel; or, the detection channel of the first detection mode is an optical detection channel, and the detection channel of the second detection mode is an impedance detection channel; or, the detection channel of the first detection mode is an optical detection channel, and the detection channel of the second detection mode is also an optical detection channel. Further, the detection channel of the first detection mode may include both an impedance detection channel and an optical detection channel; the detection channel of the second detection mode may include both an impedance detection channel and an optical detection channel.

[0010] Furthermore, the first sample to be tested and the second sample to be tested are the same or different. Specifically, for example, when the detection channel of the first detection mode and the detection channel of the second detection mode are the same, the first sample to be tested and the second sample to be tested may be the same or different. For another example, when the detection channel of the first detection mode and the detection channel of the second detection mode are different, the first sample to be tested and the second sample to be tested are generally different samples; in some special cases, the same sample may also be used for detection in two different detection channels.

[0011] Furthermore, the theoretical value of the second statistic is It is calculated by the following formula: ;in: The preset platelet counting particle number threshold; The supply quantity of the sample to be tested reaches the preset first statistic Count the number of platelet particles at the time.

[0012] In this embodiment, the second statistic is greater than or equal to the theoretical value of the second statistic. ; Push the second sample to be tested into the detection channel according to the third statistic, and output the third platelet detection result.

[0013] In this way, it can ensure that the number of platelet particles in the test sample of the second statistic is greater than or equal to the preset platelet statistical particle number threshold while not excessively increasing the statistic of the test sample, thereby effectively improving the detection efficiency.

[0014] Furthermore, the theoretical value of the second statistic Round up to get the actual value of the second statistic The specific calculation formula is as follows: ; is the theoretical value of the second statistic; “[]” is the rounding function; is the minimum measurement value of the statistic; preferably, the second statistic is the actual value of the second statistic Or the theoretical value of the second statistic ; The second sample to be tested is calculated according to the actual value of the second statistic Or the theoretical value of the second statistic Push it into the detection channel and output the third platelet detection result.

[0015] Since the actual value of the second statistic The minimum measurement value of the statistic is introduced in the calculation process , which takes into account the sample pushing accuracy of the power device, thereby ensuring that the statistics of the second sample to be tested supplied to the detection channel are more accurate, thereby ensuring the accuracy of subsequent detection results.

[0016] Furthermore, if the second statistic is greater than a preset third statistic, the first test sample is supplied to the detection channel for detection according to the third statistic, and a third platelet detection result is output.

[0017] For some special low-value platelet samples, it is possible that when the maximum statistic allowed by the detection device is supplied to the detection channel, it is still impossible to count a sufficient number of platelet particles. Therefore, in order to cope with this scenario, a third statistic is set. For example, the third statistic can be the maximum statistic allowed by the detection device, the maximum statistic of a single push of the syringe, or other statistic setting values ​​(which can be set by the operator). When the second statistic of the required second test sample calculated using the preset platelet statistical particle number threshold is greater than the preset third statistic, the second test sample is supplied to the detection channel according to the preset third statistic for detection, and the fourth platelet detection result is output. At this time, although the platelet particle amount obtained by counting is less than the preset platelet statistical particle number threshold, the platelet particle amount obtained by counting is still the maximum platelet particle amount that can be obtained by counting the second test sample currently prepared, so as to maximize the detection accuracy of the fourth platelet detection result.

[0018] Optionally, if the second statistic is greater than a preset third statistic, a prompt message is output, or the first test sample is supplied to the detection channel for detection according to the third statistic, and a third platelet detection result and a prompt message are output.

[0019] Optionally, if the second statistic is greater than the third statistic, the blood sample is retested.

[0020] Furthermore, a third sample to be tested is prepared using the blood sample for retesting, and the concentration of the third sample to be tested is greater than the concentration of the second sample to be tested.

[0021] Furthermore, before performing the platelet detection of the blood sample, a start instruction of the first detection mode or the second detection mode is triggered by a user or automatically.

[0022] Furthermore, the start instruction of the first detection mode or the second detection mode is triggered by the user. If the user triggers the start instruction of the first detection mode, the first detection mode is enabled to detect the blood sample; if the user triggers the start instruction of the second detection mode, the second detection mode is enabled to detect the blood sample.

[0023] Furthermore, before enabling the first detection mode or the second detection mode, it also includes: obtaining a click instruction of a visual operation interface, or a trigger instruction of a control panel; the visual operation interface displays a first detection mode option and a second detection mode option; the control panel is provided with a first detection mode button and a second detection mode button; wherein, enabling the first detection mode or the second detection mode includes: when the click instruction is a click instruction for the first detection mode option, or when the trigger instruction is a trigger instruction for the first detection mode button, enabling the first detection mode; when the click instruction is a click instruction for the second detection mode option, or when the trigger instruction is a trigger instruction for the second detection mode button, enabling the second detection mode.

[0024] Furthermore, the start instruction of the first detection mode or the second detection mode is automatically triggered. When the current blood sample executes the first detection mode to complete the detection, or the current sample executes the second detection mode to complete the detection, and the statistic of the second test sample supplied to the detection channel when the detection is stopped is less than the first statistic threshold, the start instruction of the first detection mode is automatically triggered when the platelet detection of the subsequent blood sample is executed; otherwise, the start instruction of the second detection mode is automatically triggered.

[0025] The present invention also proposes a computer-readable storage medium, including a program, which can be executed by a processor to implement the dynamic and efficient platelet detection method as described above.

[0026] The present invention also proposes a sample detection device, comprising: a sampling and distribution module, a reagent supply unit, a mixing chamber, a detection module and a power device; the sampling and distribution module is configured to collect blood samples and supply the blood samples at least partially to the mixing chamber; the reagent supply unit is used to supply reagents to the mixing chamber; the mixing chamber is used to receive the blood sample distributed by the sampling and distribution module and the reagent provided by the reagent supply unit to prepare a sample to be tested; the detection module is an optical detection module or an impedance detection module, the optical detection module has an optical detection channel, and the impedance detection module has an impedance detection channel; the power device is used to supply the sample to be tested to the optical detection channel or the impedance detection channel; a mode setting unit is used to enable a first mode, or to enable a second mode; the detection device also includes a processor, which is used to execute the program in a computer-readable storage medium as described above.

[0027] The present invention also proposes a dynamic and efficient platelet detection method, which uses a blood sample to prepare a second sample to be tested, uses a power device to supply the second sample to be tested to the detection channel, first supplies the second sample to be tested to the detection channel according to a preset first statistic for detection, obtains a detection signal of the second sample to be tested, obtains second platelet detection data, calculates the required second statistic of the second sample to be tested using a preset platelet statistical particle number threshold and the second platelet detection data, pushes the second sample to be tested into the detection channel according to the calculated second statistic, and outputs a third platelet detection result; Wherein, the second platelet detection data at least includes the statistical platelet particle count when the supply amount of the second sample to be tested reaches a preset first statistical amount.

[0028] Wherein, the number of platelet particles in the second test sample of the second statistic is greater than or equal to a preset platelet statistical particle number threshold.

[0029] Among them, the preset platelet particle count threshold is a set platelet particle count lower limit. When it is lower than the lower limit, the number of platelet particles counted in the detection channel is insufficient, and the obtained detection signal is also insufficient, and there is a situation where the accuracy of the platelet detection result is not high. Therefore, it is necessary to ensure that the number of platelet particles counted in the detection channel is not lower than the set platelet particle count lower limit.

[0030] In this scheme, by pre-acquiring the detection signal of the second test sample of the first statistic and obtaining the second platelet detection data, and then using the preset platelet statistical particle number threshold and the second platelet detection data to calculate the second statistic of the required second test sample, it can be ensured that the total number of PLT particles counted is always not less than the preset platelet statistical particle number threshold, and sufficient detection signals are obtained, and ultimately the detection precision and accuracy of the platelet detection results are ensured. At the same time, the judgment and identification steps of low-value platelet samples are omitted, the program flow is simplified, and the detection efficiency is improved. For some high-value samples, the second statistic calculated using the preset platelet statistical particle number threshold and the corresponding detection data may be lower than the fourth statistic set in the traditional method, and the platelet detection of the current sample is completed in advance, improving the overall detection efficiency of the instrument.

[0031] Furthermore, the theoretical value of the second statistic is The following formula is used to calculate: ;in: The preset platelet counting particle number threshold; The supply quantity of the sample to be tested reaches the preset first statistic Count the number of platelet particles at the time.

[0032] In this embodiment, the second statistic is greater than or equal to the theoretical value of the second statistic. ; Push the second sample to be tested into the detection channel according to the third statistic, and output the third platelet detection result.

[0033] In this way, it can ensure that the number of platelet particles in the test sample of the second statistic is greater than or equal to the preset platelet statistical particle number threshold while not excessively increasing the statistic of the test sample, thereby effectively improving the detection efficiency.

[0034] Furthermore, the theoretical value of the second statistic Round up to get the actual value of the second statistic , the specific calculation formula is as follows: ; is the theoretical value of the second statistic; “[]” is the rounding function; is the minimum measurement value of the statistic; preferably, the second statistic is the actual value of the second statistic Or the theoretical value of the second statistic ; The second sample to be tested is calculated according to the actual value of the second statistic Or the theoretical value of the second statistic Push it into the detection channel and output the third platelet detection result.

[0035] Since the actual value of the second statistic The minimum measurement value of the statistic is introduced in the calculation process , which takes into account the sample pushing accuracy of the power device, thereby ensuring that the statistics of the second sample to be tested supplied to the detection channel are more accurate, thereby ensuring the accuracy of subsequent detection results.

[0036] Furthermore, if the second statistic is greater than a preset third statistic, the first test sample is supplied to the detection channel for detection according to the third statistic, and a third platelet detection result is output.

[0037] For some special low-value platelet samples, it is possible that when the maximum statistic allowed by the detection device is supplied to the detection channel, it is still impossible to count a sufficient number of platelet particles. Therefore, in order to cope with this scenario, a third statistic is set. For example, the third statistic can be the maximum statistic allowed by the detection device, the maximum statistic of a single push of the syringe, or other statistic setting values ​​(which can be set by the operator). When the second statistic of the required second test sample calculated using the preset platelet statistical particle number threshold is greater than the preset third statistic, the second test sample is supplied to the detection channel according to the preset third statistic for detection, and the fourth platelet detection result is output. At this time, although the platelet particle amount obtained by counting is less than the preset platelet statistical particle number threshold, the platelet particle amount obtained by counting is still the maximum platelet particle amount that can be obtained by counting the second test sample currently prepared, so as to maximize the detection accuracy of the fourth platelet detection result.

[0038] Optionally, if the second statistic is greater than a preset third statistic, a prompt message is output, or the first test sample is supplied to the detection channel for detection according to the third statistic, and a third platelet detection result and a prompt message are output.

[0039] Optionally, if the second statistic is greater than the third statistic, the blood sample is retested.

[0040] Furthermore, a third sample to be tested is prepared using the blood sample for retesting, and the concentration of the third sample to be tested is greater than the concentration of the second sample to be tested.

[0041] Furthermore, the detection channel is an impedance detection channel or an optical detection channel.

[0042] The present invention also proposes a computer-readable storage medium, including a program, wherein the program can be executed by a processor to implement the dynamic detection method for platelet detection as described above.

[0043] The present invention also proposes a sample detection device, comprising: a sampling and distribution module, a reagent supply unit, a mixing chamber, a detection module and a power device; the sampling and distribution module is configured to collect blood samples and supply the blood samples at least partially to the mixing chamber; the reagent supply unit is used to supply reagents to the mixing chamber; the mixing chamber is used to receive the blood sample distributed by the sampling and distribution module and the reagent provided by the reagent supply unit to prepare a sample to be tested; the detection module is an optical detection module or an impedance detection module, the optical detection module has an optical detection channel, and the impedance detection module has an impedance detection channel; the power device is used to supply the sample to be tested to the optical detection channel or the impedance detection channel; the detection device also includes a processor, the processor is used to execute the program in a computer-readable storage medium as described above.

[0044] The beneficial effects of the present invention are: The dynamic and efficient platelet detection method of the present invention can enable the first detection mode and the second detection mode: when the second detection mode is enabled, the detection signal of the second sample to be tested of the first statistic is obtained in advance, and the second platelet detection data (at least including the statistical platelet particle number when the supply amount of the second sample to be tested reaches the preset first statistic) is obtained, and then the second statistic of the required second sample to be tested is calculated using the preset platelet statistical particle number threshold and the second platelet detection data, which can ensure that the total number of PLT particles counted is always not less than the preset platelet statistical particle number threshold, obtain sufficient detection signals, and ultimately ensure the detection precision and accuracy of the platelet detection results. At the same time, the judgment and identification steps of low-value platelet samples are omitted, the program flow is simplified, and the detection efficiency is improved. For some high-value samples, the second statistic calculated using the preset platelet statistical particle number threshold and the corresponding detection data may be lower than the fourth statistic set in the traditional method, and the platelet detection of the current sample is completed in advance, thereby improving the overall detection efficiency of the instrument. BRIEF DESCRIPTION OF THE DRAWINGS

[0045] In order to make the purpose, technical solution and beneficial effects of the present invention clearer, the present invention provides the following drawings for illustration: Figure 1 This is a flow chart of a first embodiment of the method for dynamic detection of leukocytes of the present invention; Figure 2 This is a flow chart of a second embodiment of the method for dynamic detection of leukocytes of the present invention; Figure 3 This is a flow chart of a third embodiment of the method for dynamic detection of leukocytes of the present invention; Figure 4 This is a flow chart of the fourth embodiment of the leukocyte dynamic detection method of the present invention. DETAILED DESCRIPTION

[0046] The present invention is further described below in conjunction with the accompanying drawings and specific embodiments so that those skilled in the art can better understand the present invention and implement it, but the embodiments are not intended to limit the present invention.

[0047] like Figure 1 As shown, the platelet dynamic detection method of this embodiment includes the following steps.

[0048] S100: Activate the first detection mode or activate the second detection mode.

[0049] If the first detection mode is enabled, a first sample to be tested is prepared (ie, step S110 is executed).

[0050] Then, step S111 is performed: supplying the first sample to be tested of the fourth statistic to the detection channel, obtaining a detection signal of the first sample to be tested of the fourth statistic through the detection device, wherein the detection signal of the first sample to be tested of the fourth statistic is at least used to obtain a first platelet detection result of the blood sample.

[0051] S112: Output the first platelet detection result.

[0052] If the second detection mode is enabled, a second sample to be tested is prepared (ie, step S120 is executed).

[0053] Then, step S121 is performed: supplying a second sample to be tested with a first statistic to the detection channel. The detection signal of the second sample to be tested is obtained by the detection device, and second platelet detection data is obtained. The second platelet detection data at least includes the statistical platelet particle number when the supply amount of the second sample to be tested reaches the preset first statistic.

[0054] S122: Calculate the required second statistic. Calculate the required second statistic of the second sample to be tested using the preset platelet statistical particle number threshold and the second platelet detection data, wherein the number of platelet particles in the second sample to be tested of the second statistic is greater than or equal to the preset platelet statistical particle number threshold.

[0055] S123: Supply a second sample to be tested of a second statistic to the detection channel (specifically, by adding the original first statistic to the second statistic). Obtain a detection signal of the second sample to be tested of the second statistic through a detection device, wherein the detection signal of the second sample to be tested of the second statistic is at least used to obtain a first platelet detection result of the blood sample.

[0056] S124: Output the third platelet detection result.

[0057] Among them, the preset platelet particle count threshold is a set platelet particle count lower limit. When it is lower than the lower limit, the number of platelet particles counted in the detection channel is insufficient, and the obtained detection signal is also insufficient, and there is a situation where the accuracy of the platelet detection result is not high. Therefore, it is necessary to ensure that the number of platelet particles counted in the detection channel is not lower than the set platelet particle count lower limit.

[0058] In the second detection mode, by pre-acquiring the detection signal of the second test sample of the first statistic and obtaining the second platelet detection data, and then using the preset platelet statistical particle number threshold and the second platelet detection data to calculate the second statistic of the required second test sample, it can be ensured that the total number of PLT particles counted is always not less than the preset platelet statistical particle number threshold, and sufficient detection signals are obtained, and ultimately the detection precision and accuracy of the platelet detection results are ensured. At the same time, the judgment and identification steps of low-value platelet samples are omitted, the program flow is simplified, and the detection efficiency is improved. For some high-value samples, the second statistic calculated using the preset platelet statistical particle number threshold and the corresponding detection data may be lower than the fourth statistic set in the traditional method, and the platelet detection of the current sample is completed in advance, thereby improving the overall detection efficiency of the instrument.

[0059] In this embodiment, the number of platelet particles in the sample to be tested of the second statistic is greater than or equal to a preset platelet statistical particle number threshold. The specific second statistic is calculated in the following manner.

[0060] First, calculate the theoretical value of the second statistic , the calculation formula is as follows: ; Then, the theoretical value of the second statistic Round up to get the actual value of the second statistic : ;in: The preset platelet counting particle number threshold; The supply quantity of the sample to be tested reaches the preset first statistic The number of platelet particles is counted at the time; “[]” is the rounding function; is the minimum value of the statistic.

[0061] In this embodiment, the second statistic is set to the actual value of the second statistic ; The second sample to be tested is calculated according to the actual value of the second statistic Push it into the detection channel and output the third platelet detection result.

[0062] Since the actual value of the second statistic The calculation formula introduces the rounding function "[]" and the minimum measurement value of the statistic. , which can effectively convert the theoretical value of the second statistic obtained by theoretical calculation into Converting it into a second statistic that is actually operable is beneficial to the supply of the second statistic to the test sample of the analytical instrument, and takes into account the sample pushing accuracy of the power device, thereby ensuring that the statistics of the second test sample supplied to the detection channel are more accurate, thereby ensuring the accuracy of subsequent detection results.

[0063] In an optional implementation, the second statistic is set to the theoretical value of the second statistic ; The second sample to be tested is calculated according to the theoretical value of the second statistic Push it into the detection channel and output the third platelet detection result.

[0064] In this way, in the second detection mode, when a low-value PLT sample appears, by using the preset platelet statistical particle number threshold and the second platelet detection data to calculate the second statistic of the required second test sample, it can be ensured that the total number of PLT particles counted is always not lower than the preset platelet statistical particle number threshold, thereby fundamentally ensuring the accuracy of the test results.

[0065] In some other optional implementations, the second statistic may also be set to be greater than or equal to the theoretical value of the second statistic. Any value of .

[0066] Furthermore, in some special application scenarios, for low-value platelet samples, it is possible that when the maximum statistic allowed by the detection device is supplied to the detection channel, it is still impossible to count a sufficient number of platelet particles (for example, it is still impossible to ensure that the total number of PLT particles counted is always not lower than the preset platelet statistical particle number threshold). Therefore, in order to cope with this situation, a third statistic is set. For example, the third statistic can be the maximum statistic allowed by the detection device, the maximum statistic of a single push of the syringe, or other statistic setting values ​​(which can be manually set by the operator).

[0067] In the second detection mode, the second statistic and the preset third statistic are compared and judged, and then different subsequent steps are executed based on the judgment result, which can effectively deal with the problem of abnormal instrument failure that may occur in the following special application scenarios as described above.

[0068] Specifically, in the second detection mode, when it is judged that the second statistic is greater than the preset third statistic, the second test sample of the third statistic is supplied to the detection channel, and the fourth platelet detection result is output; in this way, the preset third statistic is supplied to the detection channel for detection, which can ensure that the maximum platelet particle number statistic is obtained under the current detection disclosure, so as to improve the detection accuracy of the fourth platelet detection result, and also avoid abnormal failure of the instrument.

[0069] like Figure 2 As shown, in another embodiment, after the required second statistic is calculated, step S125 is performed: determining whether the second statistic is greater than the third statistic.

[0070] When the second statistic is greater than the third statistic, step S126 is executed: supplying the second test sample of the third statistic into the detection channel. S127: outputting the fourth platelet detection result.

[0071] When the second statistic is less than or equal to the third statistic, step S123 is executed: supplying a second sample to be tested with the second statistic into the detection channel. S124: outputting the third platelet detection result.

[0072] In an optional embodiment, when the judgment result of step S125 is yes (i.e., the second statistic is greater than the third statistic), if the second test sample is supplied to the detection channel according to the third statistic, the obtained platelet test result may be inaccurate, and therefore, a prompt message can be directly output.

[0073] Optionally, the prompt information includes: abnormal result prompt, retest prompt, etc. The prompt information may also include corresponding test data information, for example, the theoretical value of the second statistic , the third statistic, the dilution ratio of the current sample, the estimated maximum dilution ratio of re-examination, etc.

[0074] Optionally, in this case, although the test sample is still supplied to the detection channel according to the third statistic (since the third statistic is smaller than the second statistic of the second test sample required for calculation using the preset platelet statistical particle number threshold, insufficient platelet particle counts can be counted in the detection channel), the obtained platelet test result may be inaccurate, but the platelet test result can be used as a reference result. Therefore, the second test sample can be supplied to the detection channel for testing according to the third statistic, and the fourth platelet test result and prompt information can be output for the operator's reference.

[0075] Further, in another embodiment, when the judgment result of step S125 is obtained as yes (that is, the second statistic is greater than the third statistic), a prompt message and / or a retest is output. When performing the retest, a third sample to be tested is prepared using a blood sample for retest, and the concentration of the third sample to be tested is greater than the concentration of the second sample to be tested. In this way, the platelet concentration in the third sample to be tested is greater than the platelet concentration in the second sample to be tested, and the number of platelet particles obtained by statistics of the third sample to be tested with the same statistic is greater than the number of platelet particles obtained by statistics in the second sample to be tested, that is, the detection is performed using the third sample to be tested with a higher concentration, which can ensure that when the statistic of the third sample to be tested is less than the third statistic, the preset platelet statistical particle number threshold can be reached, so that the platelet detection result that meets the accuracy requirements can be obtained. Specifically, the detection channel is an impedance detection channel or an optical detection channel. The retest can be performed in the optical detection channel, or the retest can be completed in the impedance detection channel, preferably in the optical detection channel.

[0076] In some embodiments, the first sample to be tested and the second sample to be tested may be the same or different.

[0077] In other embodiments, the detection channel of the first detection mode and the detection channel of the second detection mode may be the same or different.

[0078] In some embodiments, before performing platelet detection of a blood sample, a start instruction of the first detection mode or the second detection mode may be triggered by a user or may be automatically triggered.

[0079] When the start instruction of the first detection mode or the second detection mode is triggered by the user, if the user triggers the start instruction of the first detection mode, the first detection mode is enabled to detect the blood sample; if the user triggers the start instruction of the second detection mode, the second detection mode is enabled to detect the blood sample. Before enabling the first detection mode or the second detection mode, it also includes: obtaining a click instruction of the visual operation interface, or a trigger instruction of the control panel. The visual operation interface displays the first detection mode option and the second detection mode option; the control panel is provided with a first detection mode button and a second detection mode button. Specifically, the trigger operation when enabling the first detection mode includes: when the click instruction is a click instruction for the first detection mode option, or when the trigger instruction is a trigger instruction for the first detection mode button, the first detection mode is enabled. The trigger operation when enabling the second detection mode includes: when the click instruction is a click instruction for the second detection mode option, or when the trigger instruction is a trigger instruction for the second detection mode button, the second detection mode is enabled.

[0080] When the start instruction of the first detection mode or the second detection mode is automatically triggered, if the current blood sample executes the second detection mode to complete the detection and is a low-value platelet sample, the start instruction of the second detection mode is automatically triggered when the platelet detection of the next blood sample is executed; otherwise, the start instruction of the first detection mode is automatically triggered.

[0081] This embodiment also proposes a computer-readable storage medium, including a program, which can be executed by a processor to implement the platelet dynamic detection method described above in this embodiment.

[0082] This embodiment also proposes a sample detection device, including: a sampling distribution module, a reagent supply unit, a mixing chamber, a detection module, a power device and a mode setting unit. Specifically, the sampling distribution module is configured to collect blood samples and supply the blood samples at least partially to the mixing chamber; the reagent supply unit is used to supply reagents to the mixing chamber; the mixing chamber is used to receive the blood sample distributed by the sampling distribution module and the reagent provided by the reagent supply unit to prepare the sample to be tested; the detection module is an optical detection module or an impedance detection module, the optical detection module has an optical detection channel, and the impedance detection module has an impedance detection channel; the power device is used to supply the sample to be tested to the optical detection channel or the impedance detection channel; the mode setting unit is used to enable the first mode, or enable the second mode; the detection device also includes a processor, the processor is used to execute the program in a computer-readable storage medium as described above in this embodiment.

[0083] In this embodiment, the detection channel is an impedance detection channel or an optical detection channel.

[0084] like Figure 3 As shown, a platelet dynamic detection method includes the following steps.

[0085] S220: preparing a second sample to be tested.

[0086] S221: Supply a second sample to be tested with a first statistic to the detection channel. Obtain a detection signal of the second sample to be tested through a detection device, and obtain second platelet detection data. The second platelet detection data at least includes the number of platelet particles when the supply amount of the second sample to be tested reaches a preset first statistic.

[0087] S222: Calculate the required second statistic. Calculate the required second statistic of the second sample to be tested using the preset platelet statistical particle number threshold and the second platelet detection data, wherein the number of platelet particles in the second sample to be tested of the second statistic is greater than or equal to the preset platelet statistical particle number threshold.

[0088] S223: Supply a second sample to be tested of a second statistic to the detection channel (specifically, by adding the original first statistic to the second statistic). Obtain a detection signal of the second sample to be tested of the second statistic through a detection device, wherein the detection signal of the second sample to be tested of the second statistic is at least used to obtain a first platelet detection result of the blood sample.

[0089] S224: Output the third platelet detection result.

[0090] Among them, the preset platelet particle count threshold is a set platelet particle count lower limit. When it is lower than the lower limit, the number of platelet particles counted in the detection channel is insufficient, and the obtained detection signal is also insufficient, and there is a situation where the accuracy of the platelet detection result is not high. Therefore, it is necessary to ensure that the number of platelet particles counted in the detection channel is not lower than the set platelet particle count lower limit.

[0091] By pre-acquiring the detection signal of the second sample to be tested of the first statistic and obtaining the second platelet detection data, and then using the preset platelet statistical particle number threshold and the second platelet detection data to calculate the second statistic of the required second sample to be tested, it can be ensured that the total number of PLT particles counted is always not less than the preset platelet statistical particle number threshold, sufficient detection signals are obtained, and ultimately the detection precision and accuracy of the platelet detection results are ensured. At the same time, the judgment and identification steps of low-value platelet samples are omitted, the program flow is simplified, and the detection efficiency is improved. For some high-value samples, the second statistic calculated using the preset platelet statistical particle number threshold and the corresponding detection data may be lower than the fourth statistic set in the traditional method, and the platelet detection of the current sample is completed in advance, improving the overall detection efficiency of the instrument.

[0092] In this embodiment, the number of platelet particles in the sample to be tested of the second statistic is greater than or equal to a preset platelet statistical particle number threshold. The specific second statistic is calculated in the following manner.

[0093] First, calculate the theoretical value of the second statistic , the calculation formula is as follows: ; Then, the theoretical value of the second statistic Round up to get the actual value of the second statistic : ;in: The preset platelet counting particle number threshold; The supply quantity of the sample to be tested reaches the preset first statistic The number of platelet particles is counted at the time; “[]” is the rounding function; is the minimum value of the statistic.

[0094] In this embodiment, the second statistic is set to the actual value of the second statistic ; The second sample to be tested is calculated according to the actual value of the second statistic Push it into the detection channel and output the third platelet detection result.

[0095] Since the actual value of the second statistic The calculation formula introduces the rounding function "[]" and the minimum measurement value of the statistic. , which can effectively convert the theoretical value of the second statistic obtained by theoretical calculation into Converting it into a second statistic that is actually operable is beneficial to the supply of the second statistic to the test sample of the analytical instrument, and takes into account the sample pushing accuracy of the power device, thereby ensuring that the statistics of the second test sample supplied to the detection channel are more accurate, thereby ensuring the accuracy of subsequent detection results.

[0096] In an optional implementation, the second statistic is set to the theoretical value of the second statistic ; The second sample to be tested is calculated according to the theoretical value of the second statistic Push it into the detection channel and output the third platelet detection result.

[0097] In this way, when a low-value PLT sample appears, by using the preset platelet statistical particle number threshold and the second platelet detection data to calculate the second statistic of the required second test sample, it can be ensured that the total number of PLT particles counted is always not lower than the preset platelet statistical particle number threshold, thereby fundamentally ensuring the accuracy of the test results.

[0098] In some other optional implementations, the second statistic may also be set to be greater than or equal to the theoretical value of the second statistic. Any value of .

[0099] Furthermore, in some special application scenarios, for low-value platelet samples, it is possible that when the maximum statistic allowed by the detection device is supplied to the detection channel, it is still impossible to count a sufficient number of platelet particles (for example, it is still impossible to ensure that the total number of PLT particles counted is always not lower than the preset platelet statistical particle number threshold). Therefore, in order to deal with this situation, a third statistic is set. For example, the third statistic can be the maximum statistic allowed by the detection device, the maximum statistic of a single push of the syringe, or other statistic setting values ​​(which can be manually set by the operator). By comparing and judging the second statistic and the preset third statistic, and then executing different subsequent steps based on the judgment result, it is possible to effectively deal with the problem of abnormal instrument failure that may occur in the following special application scenarios as described above.

[0100] Specifically, when it is judged that the second statistic is greater than the preset third statistic, the second test sample of the third statistic is supplied to the detection channel, and the fourth platelet detection result is output; in this way, the preset third statistic is supplied to the detection channel for detection, which can ensure that the maximum platelet particle number statistic is obtained under the current detection disclosure, so as to improve the detection accuracy of the fourth platelet detection result, and also avoid abnormal failure of the instrument.

[0101] like Figure 4 As shown, in another embodiment, after the required second statistic is calculated, step S225 is executed: determining whether the second statistic is greater than the third statistic.

[0102] When the second statistic is greater than the third statistic, step S226 is executed: supplying the second test sample of the third statistic into the detection channel. S227: outputting the fourth platelet detection result.

[0103] When the second statistic is less than or equal to the third statistic, step S223 is executed: supplying the second test sample of the second statistic into the detection channel. S224: outputting the third platelet detection result.

[0104] In an optional embodiment, when the judgment result of step S225 is yes (i.e., the second statistic is greater than the third statistic), if the second test sample is supplied to the detection channel according to the third statistic, the obtained platelet test result may be inaccurate, and therefore, a prompt message can be directly output.

[0105] Optionally, the prompt information includes: abnormal result prompt, retest prompt, etc. The prompt information may also include corresponding test data information, for example, the theoretical value of the second statistic , the third statistic, the dilution ratio of the current sample, the estimated maximum dilution ratio of re-examination, etc.

[0106] Optionally, in this case, although the test sample is still supplied to the detection channel according to the third statistic (since the third statistic is smaller than the second statistic of the second test sample required for calculation using the preset platelet statistical particle number threshold, insufficient platelet particle counts can be counted in the detection channel), the obtained platelet test result may be inaccurate, but the platelet test result can be used as a reference result. Therefore, the second test sample can be supplied to the detection channel for testing according to the third statistic, and the fourth platelet test result and prompt information can be output for the operator's reference.

[0107] Further, in another embodiment, when the judgment result of step S225 is obtained as yes (that is, the second statistic is greater than the third statistic), a prompt message and / or a retest is output. When performing the retest, a third sample to be tested is prepared using a blood sample for retest, and the concentration of the third sample to be tested is greater than the concentration of the second sample to be tested. In this way, the platelet concentration in the third sample to be tested is greater than the platelet concentration in the second sample to be tested, and the number of platelet particles obtained by statistics of the third sample to be tested with the same statistic is greater than the number of platelet particles obtained by statistics in the second sample to be tested, that is, the detection is performed using the third sample to be tested with a higher concentration, which can ensure that when the statistic of the third sample to be tested is less than the third statistic, the preset platelet statistical particle number threshold can be reached, so that the platelet detection result that meets the accuracy requirements can be obtained. Specifically, the detection channel is an impedance detection channel or an optical detection channel. The retest can be performed in the optical detection channel, or the retest can be completed in the impedance detection channel, preferably in the optical detection channel.

[0108] This embodiment also proposes a computer-readable storage medium, including a program, which can be executed by a processor to implement the platelet dynamic detection method described above in this embodiment.

[0109] This embodiment also proposes a sample detection device, including: a sampling distribution module, a reagent supply unit, a mixing chamber, a detection module, a power device and a mode setting unit. Specifically, the sampling distribution module is configured to collect blood samples and supply the blood samples at least partially to the mixing chamber; the reagent supply unit is used to supply reagents to the mixing chamber; the mixing chamber is used to receive the blood sample distributed by the sampling distribution module and the reagent provided by the reagent supply unit to prepare the sample to be tested; the detection module is an optical detection module or an impedance detection module, the optical detection module has an optical detection channel, and the impedance detection module has an impedance detection channel; the power device is used to supply the sample to be tested to the optical detection channel or the impedance detection channel; the mode setting unit is used to enable the first mode, or enable the second mode; the detection device also includes a processor, the processor is used to execute the program in a computer-readable storage medium as described above in this embodiment.

[0110] In this embodiment, the detection channel is an impedance detection channel or an optical detection channel.

[0111] The above-described embodiments are only preferred embodiments for fully illustrating the present invention, and the protection scope of the present invention is not limited thereto. Equivalent substitutions or changes made by those skilled in the art based on the present invention are within the protection scope of the present invention. The protection scope of the present invention shall be subject to the claims.

Claims

1. A dynamic detection method for platelet detection, characterized in that: The method comprises: Enable the first detection mode or the second detection mode; When the first detection mode is enabled: A first sample to be tested is prepared using a blood sample, and the first sample to be tested is supplied to a detection channel for testing according to a preset fourth statistic, and a detection signal of the first sample to be tested is obtained, wherein the detection signal of the first sample to be tested is at least used to obtain a first platelet test result of the blood sample, and the first platelet test result is output; When the second detection mode is enabled: A second sample to be tested is prepared using a blood sample, and the second sample to be tested is supplied to the detection channel using a power device. The second sample to be tested is first supplied to the detection channel for detection according to a preset first statistic, and a detection signal of the second sample to be tested is obtained to obtain second platelet detection data, and a second statistic of the required second sample to be tested is calculated using a preset platelet statistical particle number threshold and the second platelet detection data, and the second sample to be tested is pushed into the detection channel according to the calculated second statistic, and a third platelet detection result is output; Wherein, the second platelet detection data at least includes the statistical platelet particle count when the supply amount of the second sample to be tested reaches a preset first statistical amount; Wherein, the number of platelet particles in the second test sample of the second statistic is greater than or equal to a preset platelet statistical particle number threshold.

2. The dynamic detection method for platelet detection according to claim 1, characterized in that: The detection channel is an impedance detection channel or an optical detection channel; and / or, the first sample to be tested and the second sample to be tested are the same or different.

3. The dynamic detection method for platelet detection according to any one of claims 1 or 2, characterized in that: The theoretical value of the second statistic It is calculated by the following formula: ;in: The preset platelet counting particle number threshold; The supply quantity of the sample to be tested reaches the preset first statistic Count the number of platelet particles at the time of Among them, the second statistic is greater than or equal to the theoretical value of the second statistic ; The second sample to be tested is pushed into the detection channel according to the third statistic, and the third platelet detection result is output.

4. The dynamic detection method for platelet detection according to claim 3, characterized in that: The actual value of the second statistic It is calculated by the following formula: ;in: is the theoretical value of the second statistic; "[]" is the rounding function; is the minimum measurement value of the statistic; Among them, the second statistic is the actual value of the second statistic Or the theoretical value of the second statistic ; The second sample to be tested is calculated according to the actual value of the second statistic Or the theoretical value of the second statistic Push it into the detection channel and output the third platelet detection result.

5. The dynamic detection method for platelet detection according to claim 4, characterized in that: If the second statistic is greater than the preset third statistic, the second test sample is supplied to the detection channel for detection according to the preset third statistic, and a fourth platelet detection result is output.

6. The dynamic detection method for platelet detection according to claim 4, characterized in that: If the second statistic is greater than the preset third statistic, a prompt message is output, or the second test sample is supplied to the detection channel for detection according to the preset third statistic, and a fourth platelet detection result and a prompt message are output.

7. The dynamic detection method for platelet detection according to claim 4, characterized in that: If the second statistic is greater than the preset third statistic, the blood sample is retested.

8. The dynamic detection method for platelet detection according to claim 7, characterized in that: A third sample to be tested is prepared using the blood sample for retesting, and the concentration of the third sample to be tested is greater than the concentration of the second sample to be tested.

9. The dynamic detection method for platelet detection according to any one of claims 1 or 2, characterized in that: Before performing the platelet test on the blood sample, a start instruction for the first test mode or the second test mode is triggered by a user or automatically.

10. The dynamic detection method for platelet detection according to claim 9, characterized in that: The start instruction of the first detection mode or the second detection mode is triggered by the user. If the user triggers the start instruction of the first detection mode, the first detection mode is enabled to detect the blood sample; If the user triggers the start instruction of the second detection mode, the second detection mode is enabled to detect the blood sample.

11. The dynamic detection method for platelet detection according to claim 10, characterized in that: Before enabling the first detection mode or the second detection mode, it also includes: obtaining a click instruction of a visual operation interface, or a trigger instruction of a control panel; the visual operation interface displays the first detection mode option and the second detection mode option; the control panel is provided with a first detection mode button and a second detection mode button; wherein, enabling the first detection mode or the second detection mode includes: when the click instruction is a click instruction for the first detection mode option, or when the trigger instruction is a trigger instruction for the first detection mode button, enabling the first detection mode; when the click instruction is a click instruction for the second detection mode option, or when the trigger instruction is a trigger instruction for the second detection mode button, enabling the second detection mode.

12. The dynamic detection method for platelet detection according to claim 9, characterized in that: The start instruction of the first detection mode or the second detection mode is automatically triggered. When the current blood sample executes the second detection mode to complete the detection and is a low-value platelet sample, the start instruction of the second detection mode is automatically triggered when the platelet detection of the subsequent blood sample is executed; otherwise, the start instruction of the first detection mode is automatically triggered.

13. A computer-readable storage medium, characterized in that: The invention comprises a program which can be executed by a processor to implement the dynamic detection method for platelet detection as claimed in any one of claims 1 to 12.

14. A sample detection device, comprising: A sampling and distribution module, a reagent supply unit, a mixing chamber, a detection module, a power device and a mode setting unit; characterized in that the sampling and distribution module is configured to collect a blood sample and supply the blood sample at least partially to the mixing chamber; the reagent supply unit is used to supply the reagent to the mixing chamber; the mixing chamber is used to receive the blood sample distributed by the sampling and distribution module and the reagent provided by the reagent supply unit to prepare a sample to be tested; the detection module is an optical detection module or an impedance detection module, the optical detection module has an optical detection channel, and the impedance detection module has an impedance detection channel; the power device is used to supply the sample to be tested to the optical detection channel or the impedance detection channel; a mode setting unit is used to enable a first mode, or to enable a second mode; the detection device also includes a processor, the processor is used to execute the program in a computer-readable storage medium as described in claim 13.

15. A dynamic detection method for platelet detection, characterized in that: The method comprises: A second sample to be tested is prepared using a blood sample, and the second sample to be tested is supplied to the detection channel using a power device. The second sample to be tested is first supplied to the detection channel for detection according to a preset first statistic, and a detection signal of the second sample to be tested is obtained to obtain second platelet detection data, and a second statistic of the required second sample to be tested is calculated using a preset platelet statistical particle number threshold and the second platelet detection data, and the second sample to be tested is pushed into the detection channel according to the calculated second statistic, and a third platelet detection result is output; Wherein, the second platelet detection data at least includes the statistical platelet particle count when the supply amount of the second sample to be tested reaches a preset first statistical amount; Wherein, the number of platelet particles in the second test sample of the second statistic is greater than or equal to a preset platelet statistical particle number threshold.

16. The dynamic detection method for platelet detection according to claim 15, characterized in that: The theoretical value of the second statistic It is calculated by the following formula: ;in: The preset platelet counting particle number threshold; The supply quantity of the sample to be tested reaches the preset first statistic Count the number of platelet particles at the time of Among them, the second statistic is greater than or equal to the theoretical value of the second statistic ; The second sample to be tested is pushed into the detection channel according to the third statistic, and the third platelet detection result is output.

17. The dynamic detection method for platelet detection according to claim 16, characterized in that: The actual value of the second statistic It is calculated by the following formula: ;in: is the theoretical value of the second statistic; "[]" is the rounding function; is the minimum measurement value of the statistic; Among them, the second statistic is the actual value of the second statistic Or the theoretical value of the second statistic ; The second sample to be tested is calculated according to the actual value of the second statistic Or the theoretical value of the second statistic Push it into the detection channel and output the third platelet detection result.

18. The dynamic detection method for platelet detection according to any one of claims 15 to 17, characterized in that: If the second statistic is greater than the preset third statistic, the second test sample is supplied to the detection channel for detection according to the preset third statistic, and a fourth platelet detection result is output.

19. The dynamic detection method for platelet detection according to any one of claims 15 to 17, characterized in that: If the second statistic is greater than the preset third statistic, a prompt message is output, or the second test sample is supplied to the detection channel for detection according to the preset third statistic, and a fourth platelet detection result and a prompt message are output.

20. The dynamic detection method for platelet detection according to any one of claims 15 to 17, characterized in that: If the second statistic is greater than the preset third statistic, the blood sample is retested.

21. The dynamic detection method for platelet detection according to claim 20, characterized in that: A third sample to be tested is prepared using the blood sample for retesting, and the concentration of the third sample to be tested is greater than the concentration of the second sample to be tested.

22. The dynamic detection method for platelet detection according to any one of claims 15 to 17, characterized in that: The detection channel is an impedance detection channel and / or an optical detection channel.

23. A computer-readable storage medium, characterized in that: It comprises a program which can be executed by a processor to implement the dynamic detection method for platelet detection as described in any one of claims 16-22.

24. A sample detection device, comprising: A sampling and distribution module, a reagent supply unit, a mixing chamber, a detection module and a power device; characterized in that the sampling and distribution module is configured to collect blood samples and supply the blood samples at least partially to the mixing chamber; the reagent supply unit is used to supply reagents to the mixing chamber; the mixing chamber is used to receive the blood samples distributed by the sampling and distribution module and the reagents provided by the reagent supply unit to prepare the samples to be tested; the detection module is an optical detection module or an impedance detection module, the optical detection module has an optical detection channel, and the impedance detection module has an impedance detection channel; the power device is used to supply the samples to be tested to the optical detection channel or the impedance detection channel; the detection device also includes a processor, the processor is used to execute the program in a computer-readable storage medium as described in claim 23.

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