Application of ascorbic acid peroxidase in relieving high temperature stress of rice

Abstract

The invention discloses application of ascorbic acid peroxidase (APX) in relieving high-temperature stress of rice, and APX treatment can relieve damage of high-temperature stress to the rice and can relieve the reduction effect of the high-temperature stress on the survival rate of rice seedlings and the yield of a single plant. Compared with high-temperature treatment, 100 [mu] g / L APX treatment can improve the survival rate of rice seedlings by 61% and the yield per plant of rice by 42% under high-temperature stress by relieving the damage of high-temperature stress to the rice.

Description

Technical Field

[0001] The invention belongs to the technical field of plant growth and development regulation, and particularly relates to the application of ascorbate peroxidase in alleviating high temperature stress of rice. Background Art

[0002] Rice (Oryza sativa L.) is the most important food crop in the world and plays a very important role in global food production. However, with global warming, frequent high temperatures have brought great challenges to rice production. High temperatures not only affect the growth and development of rice, but also reduce rice yield and quality. Therefore, the development of regulatory technologies that can alleviate high temperature stress in rice is of great significance and application prospects.

[0003] Ascorbate peroxidase (APX) is an enzyme that can be synthesized by plants themselves. After searching at home and abroad, there are currently no patents and literature reports on alleviating rice high temperature stress by applying exogenous APX. The present invention improves the ability of rice to resist high temperature stress by applying exogenous APX, thereby improving the survival rate and yield of rice under high temperature stress. Summary of the invention

[0004] The present invention provides an application of APX in alleviating high temperature stress of rice. APX treatment can alleviate the reducing effect of high temperature stress on the survival rate of rice seedlings and can alleviate the yield loss of rice caused by high temperature stress.

[0005] APX is used to alleviate the effect of high temperature stress on the survival rate of rice seedlings: compared with high temperature treatment, 1 μg / L APX treatment can alleviate the effect of high temperature stress on the survival rate of rice seedlings. As the concentration of APX treatment increases, the effect of APX on alleviating rice high temperature stress increases. APX has a significant effect of alleviating high temperature stress in the range of 10-200 μg / L. Preferably, the survival rate of rice seedlings under high temperature stress after 100 μg / L APX hydroponic treatment is as high as 86%.

[0006] APX is used to alleviate the reducing effect of high temperature stress on rice yield: compared with normal temperature treatment, high temperature treatment significantly reduced the yield of each test rice plant; compared with high temperature treatment, the yield of each rice plant under high temperature stress increased by 42% after spraying 100μg / L APX.

[0007] In order to solve the problem of high temperature stress harming rice production, APX is applied to enhance the heat tolerance of rice, thereby alleviating the damage caused by high temperature stress to rice. The application of APX has the advantages of good effect, simple and quick operation, and is an ideal method to alleviate the high temperature stress of rice. BRIEF DESCRIPTION OF THE DRAWINGS

[0008] Figure 1.The effect of 100 μg / L APX in Example 1 on alleviating high temperature stress in rice. DETAILED DESCRIPTION

[0009] Example 1: APX alleviates the effect of high temperature stress on the survival rate of rice seedlings

[0010] DongJin (DJ) was used as the experimental material to verify the effect of different concentrations of APX in alleviating high temperature stress of rice. The method is:

[0011] Normal temperature treatment: DJ seedlings with uniform germination were selected, and cultured with the International Rice Research Institute (IRRI) rice formula nutrient solution at normal temperature (29°C, light during the day; 26°C, dark at night) for 19 days + 90 hours, and then statistical data (survival rate) was collected.

[0012] High temperature treatment: DJ seedlings with uniform germination were selected, cultured at room temperature for 7 days using the International Rice Research Institute (IRRI) rice formula nutrient solution, and then subjected to high temperature treatment (43°C, light during the day; 43°C, darkness at night) for 90 hours. Statistical data (survival rate) were collected after returning to normal temperature for 12 days.

[0013] Normal temperature + APX treatment: Select DJ seedlings with uniform germination, culture them at normal temperature for 4 days using the International Rice Research Institute (IRRI) rice formula nutrient solution, pre-treat them at normal temperature for 3 days using different concentrations of APX (1, ​​10, 50, 100, 150, 200, 400 μg / L), and then culture them at normal temperature for 12 days + 90 hours before statistical data (survival rate) are calculated.

[0014] High temperature + APX treatment: DJ seedlings with uniform germination were selected, cultured at room temperature for 4 days using the International Rice Research Institute (IRRI) rice formula nutrient solution, pretreated at room temperature for 3 days using different concentrations of APX (1, ​​10, 50, 100, 150, 200, 400 μg / L), then treated at high temperature for 90 hours, and statistical data (survival rate) were calculated after 12 days of recovery to room temperature.

[0015] Different concentrations of APX (1, ​​10, 50, 100, 150, 200, 400 μg / L) were used for pretreatment by hydroponics. The hydroponic solution contained the International Rice Research Institute (IRRI) rice nutrient solution formula.

[0016] Table 1 APX alleviates the reduction effect of high temperature stress on rice seedling survival rate

[0017]

[0018]

[0019] As shown in Table 1, high temperature stress can cause serious damage to the vegetative growth of rice, resulting in a decrease in the survival rate of rice seedlings. APX treatment can alleviate the damage caused by high temperature stress to rice seedlings, thereby alleviating the effect of high temperature stress on the survival rate of rice seedlings. Even at a low APX treatment concentration (1 μg / L), APX can alleviate the high temperature stress of rice seedlings. With the increase of APX treatment concentration, the effect of APX on alleviating the high temperature stress of rice seedlings is enhanced. However, excessively high concentrations of APX treatment will weaken the effect of APX on alleviating the high temperature stress of rice seedlings. Compared with high temperature treatment, APX treatment can improve the survival rate of rice seedlings under high temperature stress by 4% to 61% by alleviating the damage caused by high temperature stress to rice seedlings.

[0020] Example 2: APX alleviates the reducing effect of high temperature stress on rice yield

[0021] The experiment was conducted using DongJin (DJ) as the experimental material, and the method was as follows:

[0022] Normal temperature treatment: During the flowering period of rice, select test rice with consistent growth as experimental materials, use H2O as a control to spray the test rice at normal temperature for 3 days, 2 times / day, and then wait for the rice to mature under normal temperature conditions to calculate the single plant yield and the reduction in single plant yield.

[0023] Normal temperature + APX treatment: During the flowering period of rice, select the test rice with consistent growth as the experimental material, spray the test rice with 100μg / L APX at normal temperature for 3 days, 2 times / day, and then wait for the rice to mature under normal temperature conditions to calculate the single plant yield and the reduction in single plant yield.

[0024] High temperature treatment: During the flowering period of rice, select test rice with consistent growth as experimental materials, use H2O as a control and spray the test rice at normal temperature for 3 days, twice a day, followed by high temperature (44°C, light) treatment for 6 hours, and then wait for the rice to mature under normal temperature conditions to calculate the single plant yield and the degree of reduction in single plant yield.

[0025] High temperature + APX treatment: During the flowering period of rice, select the test rice with consistent growth as the experimental material, spray the test rice with 100μg / L APX at room temperature for 3 days, twice a day, and then treat with high temperature (44℃, light) for 6h, and then wait for the rice to mature under normal temperature conditions to calculate the single plant yield and the reduction in single plant yield.

[0026] Table 2 APX alleviates the reduction effect of high temperature stress on rice yield

[0027]

[0028] As shown in Table 2, high temperature stress can cause serious damage to the reproductive growth of rice. Compared with the normal temperature treatment, the rice yield of high temperature treatment was significantly reduced; compared with the high temperature treatment, the rice yield of high temperature + APX treatment was significantly increased. 100μg / L APX treatment can significantly alleviate the effect of high temperature on the yield reduction of the tested rice.

[0029] In summary, high temperature stress can cause damage to rice, reduce rice survival rate and rice yield; APX treatment can alleviate the damage caused by high temperature stress to rice, thereby increasing the survival rate and rice yield of rice under high temperature stress. Even at a low APX treatment concentration (1 μg / L), APX can alleviate rice high temperature stress. As the APX treatment concentration increases, the effect of APX on alleviating rice high temperature stress increases. However, excessively high concentrations of APX treatment will weaken the effect of APX on alleviating rice high temperature stress. Compared with high temperature treatment, 100 μg / L APX treatment can alleviate the damage caused by high temperature stress to rice, thereby increasing the survival rate of rice seedlings by 61% and the yield of rice per plant by 42% under high temperature stress.

Claims

1. Application of ascorbate peroxidase in alleviating high temperature stress in rice.

2. The use according to claim 1, characterized in that: The ascorbate peroxidase is used at a concentration of 10-200 μg / L.

3. The use according to claim 2, characterized in that: The ascorbate peroxidase is used at a concentration of 50-150 μg / L.

4. The use according to claim 2 or 3, characterized in that: Ascorbate peroxidase is applied hydroponically or by spraying.

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