Special PCR (Polymerase Chain Reaction) analysis instrument for gene analysis

Abstract

The utility model discloses a special PCR (Polymerase Chain Reaction) analytical instrument for gene analysis, which relates to the technical field of gene analysis and comprises a mounting box, an automatic conveying unit and a shaking and mixing unit, the mounting box is a square box body; the automatic conveying unit is mounted on the lower side in the mounting box; the shaking and mixing unit comprises a rubber insertion frame, an insertion hole, a glass test tube, a knocker, a rubber block and a heater. The device is provided with the reliable shaking and mixing unit, and the knocker on the side surface of the containing box sequentially strikes the clamping frame to cause oscillation of substances in the glass test tube, so that enzyme and nucleic acid substances are in more sufficient contact, the effect of increasing the reaction rate is achieved, and the device can be more effectively applied to the field of gene analysis; and the automatic conveying unit is further arranged, the glass test tubes filled with gene substances can be automatically conveyed, hands of personnel can be prevented from directly touching the test tubes in the conveying-in and conveying-out processes, and good sealing and heat preservation effects are achieved.

Description

Technical Field

[0001] The utility model relates to the technical field of gene analysis, in particular to a PCR analysis instrument dedicated to gene analysis. Background Art

[0002] PCR analyzer is an analytical instrument used in biology, basic medicine, and pharmacy. It has important applications in the field of gene analysis. It uses the principle of PCR technology to analyze specific gene fragments. In experiments, it was found that DNA can also denature and melt at high temperatures, and can renature into double strands when the temperature is lowered. Therefore, by controlling the denaturation and renaturation of DNA through temperature changes, adding designed primers, DNA polymerase, and dNTP, the in vitro replication of specific genes can be completed. Based on this, the RNA sequence and other information of specific genes can be studied and analyzed;

[0003] The PCR analysis instrument of the prior art cannot be fully mixed when the nucleic acid, buffer, fluorescent reagent and corresponding enzyme solution in the test tube are at rest, which may lead to low fusion efficiency, incomplete unwinding of DNA double helix, ineffective attachment of fluorescent substance, etc., which may cause errors in analysis and detection and cannot be effectively used in the field of gene analysis. In addition, in the process of placing the research material, the test tube needs to be manually sent into the deep of the device, which is not convenient and may easily cause the research material to be contaminated. In addition, since temperature stability has an important influence on the reaction, the device needs to have good insulation and sealing effects. For this reason, we propose a PCR analysis instrument dedicated to gene analysis. Utility Model Content

[0004] The technical problem to be solved by the utility model is to overcome the existing defects and provide a PCR analysis instrument dedicated to gene analysis. The instrument has a reliable shaking and mixing unit, and controls the heating temperature of the nucleic acid material in the test tube, thereby leading the unwinding and recombination process of the DNA. The knockers on the side of the containing box hit the card mounting frame in turn, causing the material in the glass test tube to vibrate, so that the enzyme and the nucleic acid material are in contact more fully, which plays a role in increasing the reaction rate. The utility model also has a reliable automatic transport unit, which can automatically transport glass test tubes containing nucleic acids, buffer solutions, fluorescent reagents and corresponding enzyme solutions. The transport in and out processes can avoid direct contact between the test tubes by the hands of personnel, and the instrument has good sealing and heat preservation effects, so that the instrument can be more effectively used in the field of gene analysis, and can effectively solve the problems in the background technology.

[0005] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: A PCR analysis instrument dedicated to gene analysis, comprising an installation box, an automatic transport unit and a shaking and mixing unit;

[0006] Installation box: a square box with an inclined end surface on the upper front side;

[0007] Automatic transport unit: installed on the lower side of the installation box;

[0008] Shake and mix unit: comprises a rubber rack, a mounting hole, a glass test tube, a knocker, a rubber block and a heater. The holding box contained in the automatic transport unit is limitedly clamped inside the mounting frame. A rubber rack is placed inside the upper end of the holding box. The internal filling array of the rubber rack is provided with vertical mounting holes. The glass test tube passes through the mounting holes, and its bottom is inserted into the interior of the heater. The heater is fixed on the lower side of the holding box. A knocker is embedded in the middle of the four sides of the upper end frame of the holding box, and the end of the knocker extends outward and is fixedly connected to a rubber block.

[0009] The automatic transport unit automatically transports glass test tubes containing nucleic acids, buffer solutions, fluorescent reagents and corresponding enzyme solutions. The transport in and out processes can prevent people from directly touching the test tubes with their hands. The rubber rack is used for the insertion of glass test tubes. The glass test tubes are vertically inserted into the insertion holes, and the bottom of the test tubes are extended into the heater for heating. The heating temperature of the nucleic acid material in the test tube is controlled, thereby leading the DNA unwinding and recombination process. The knocker on the side of the container hits the mounting frame in turn, causing the container and the glass test tube to shake, thereby causing the material in the glass test tube to vibrate, allowing the enzyme to contact the nucleic acid material more fully, thereby increasing the reaction rate. The rubber block can hit the mounting frame at the end of the knocker to play a buffering and protective role, and reduce the noise generated by the collision.

[0010] Furthermore, the automatic transport unit comprises a fitting groove, a transport channel, a clamping slide, a hydraulic cylinder, a side slide bar and a start button. A longitudinal transport channel is provided inside the installation box, a slightly larger fitting groove is provided at the front port of the transport channel, a clamping slide is provided on the left and right side walls of the transport channel, and a side slide bar is slidably connected inside each clamping slide. Two left and right hydraulic cylinders are embedded and installed on the rear wall of the transport channel, and the telescopic end of the hydraulic cylinder is horizontally forward and fixedly connected to the tail of the side slide bar. The hydraulic pump of the hydraulic cylinder is electrically connected to the output end of the external power supply through the start button installed on the right front side of the installation box. The start button is used to control the hydraulic cylinder to work, and the hydraulic cylinder drives the clamping frame to move through the side slide bars on both sides, thereby driving the loading box to move forward and backward along the transport channel to realize the loading and feeding process.

[0011] Furthermore, the automatic transport unit further comprises a card-mounted frame, a container and a hand-carrying ring, wherein the card-mounted frame is fixedly connected between the left and right side slide bars, the card-mounted frame is a square frame body and has an L-shaped cross section, the container is limitedly clamped on the step platform formed by the L-shaped structure of the card-mounted frame, and a hand-carrying ring is fixedly connected to the upper side of the front and rear ends of the card-mounted frame. The card-mounted frame is used to limit the container, and the container is placed into the card-mounted frame from the upper side, and the hand-carrying ring can facilitate the process of loading and unloading the container.

[0012] Furthermore, the automatic transport unit further comprises a front closing plate, a sealing strip and a heat preservation plate, wherein the front closing plate is fixedly connected to the front ends of the two side sliding rods, the front closing plate corresponds longitudinally to the fitting groove and has the same size, a heat preservation plate is fixedly installed on the rear side of the front closing plate, the heat preservation plate corresponds longitudinally to the transport channel and has the same size, and a circle of sealing strip is fixedly installed near the edge of the rear side of the front closing plate. When the side sliding rod drives the card-mounted frame to be retracted into the transport channel, the front closing plate is tightly attached to the fitting groove, the sealing strip plays a good sealing role between the two, and the heat preservation plate can further prevent the heat loss inside the transport channel, thereby maintaining the stability of the heating temperature of the glass test tube.

[0013] Furthermore, it also includes a microprocessor, a data interface and a photomultiplier tube. The microprocessor is installed on the upper side of the interior of the installation box. The microprocessor is electrically connected to a touch screen. The touch screen is embedded in the inclined end surface of the upper front side of the installation box. The data interface is provided on the right side of the microprocessor. A square installation plate is provided below the microprocessor. The upper end of the installation plate is filled with a rectangular area of ​​photomultiplier tubes. The lower end of the photomultiplier tube is installed with a full-spectrum LED lamp. Each photomultiplier tube corresponds vertically to a glass test tube, and the input end of the photomultiplier tube is bidirectionally electrically connected to the upper microprocessor. The full-spectrum LED lamp emits a variety of color lights to the fluorescent reagent in the glass test tube. During the PCR process, the nucleic acid carrying the fluorescent reagent is arranged in order. The photomultiplier tube is used to convert the captured light and shadow information into an electrical signal. The microprocessor analyzes the transmitted electrical signal and displays the analysis results of the gene on the screen. The screen can be touch-controlled to control the operation of the embedded analysis program of the microprocessor. The data interface is used to connect an external data line to transmit or save the result data of the gene analysis.

[0014] Furthermore, it also includes a mounting short column and a suction cup foot. The four corners of the lower end of the mounting box are fixedly connected with a mounting short column, and the lower end of the mounting short column is provided with a suction cup foot. The mounting short column is used for installing the suction cup foot, and the suction cup foot can be sucked tightly on the placement surface, thereby ensuring the overall stability of the device during operation.

[0015] Compared with the prior art, the utility model has the following advantages: the PCR analysis instrument dedicated to gene analysis has the following advantages:

[0016] 1. It has a reliable shaking and mixing unit. The rubber insert is used to insert the glass test tube. The bottom of the test tube is inserted into the heater for heating. By controlling the heating temperature of the nucleic acid substance in the test tube, the DNA unwinding and recombination process is achieved. The knocker on the side of the container hits the card installation frame in turn, so that the container and the glass test tube are shaken, and then the substance in the glass test tube is vibrated, so that the enzyme and the nucleic acid substance are in contact more fully, which plays a role in increasing the reaction rate, so that this instrument can be more effectively used in the field of gene analysis;

[0017] 2. It has a reliable automatic transport unit, which can automatically transport glass test tubes containing nucleic acids, buffer solutions, fluorescent reagents and corresponding enzyme solutions. The transport in and out process can prevent people from directly touching the test tubes. When the side slide rod drives the card-mounted frame to retract into the transport channel, the front closing plate is tightly attached to the fitting groove, and the sealing strip plays a good sealing role between the two. The insulation plate can further prevent the loss of heat inside the transport channel, thereby maintaining the stability of the heating temperature of the glass test tube;

[0018] 3. The utility model has a reliable shaking and mixing unit. The rubber inserting rack is used for inserting glass test tubes. The bottom of the test tube is extended into the heater for heating. By controlling the heating temperature of the nucleic acid material in the test tube, the unwinding and recombination process of DNA is realized. The knocker on the side of the container hits the card installation frame in turn, so that the container and the glass test tube are shaken, and then the material in the glass test tube is vibrated, so that the enzyme and the nucleic acid material are in contact more fully, which plays a role in improving the reaction rate, so that the instrument can be more effectively used in the field of gene analysis; it also has a reliable automatic transport unit, which can automatically transport glass test tubes containing nucleic acids, buffers, fluorescent reagents and corresponding enzyme solutions. The transport in and out process can avoid direct contact between the hands of personnel. When the side slide rod drives the card installation frame to be retracted into the inside of the transport channel, the front closing plate is tightly attached to the fitting groove, and the sealing strip plays a good sealing role between the two. The insulation plate can further prevent the loss of heat inside the transport channel, thereby maintaining the stability of the heating temperature of the glass test tube. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a schematic diagram of the structure of the utility model;

[0020] Figure 2 This is a schematic diagram of the internal structure of the utility model;

[0021] Figure 3 It is a partial cross-sectional view of the utility model;

[0022] Figure 4 It is a schematic diagram of the local structure of the utility model;

[0023] Figure 5 For this utility model Figure 4 A magnified view of the structure in the middle.

[0024] In the figure: 1 installation box, 2 automatic transport unit, 21 fitting groove, 22 transport channel, 23 clamping slide, 24 hydraulic cylinder, 25 side slide bar, 26 clamping frame, 27 loading box, 28 hand ring, 29 start button, 210 front closing plate, 211 sealing strip, 212 insulation plate, 3 shaking mixing unit, 31 rubber plug rack, 32 plug hole, 33 glass test tube, 34 knocker, 35 rubber block, 36 heater, 4 microprocessor, 5 data interface, 6 photomultiplier tube, 7 installation short column, 8 suction cup foot. DETAILED DESCRIPTION

[0025] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0026] See also Figure 1-Figure 5 ,This embodiment provides a technical solution: A PCR analysis instrument dedicated to gene analysis, comprising an installation box 1, an automatic transport unit 2 and a shaking and mixing unit 3;

[0027] Installation box 1: It is a square box with an inclined end surface at the front of the upper end;

[0028] Automatic transport unit 2: installed on the lower side of the installation box 1;

[0029] The shaking and mixing unit 3 comprises a rubber rack 31, an insertion hole 32, a glass test tube 33, a knocker 34, a rubber block 35 and a heater 36. The loading box 27 contained in the automatic transport unit 2 is limitedly clamped inside the clamping frame 26. A rubber rack 31 is placed inside the upper end of the loading box 27. The internal filling array of the rubber rack 31 is provided with a vertical insertion hole 32. The glass test tube 33 passes through the insertion hole 32, and its bottom is inserted into the interior of the heater 36. The heater 36 is installed and fixed on the lower side of the loading box 27. A knocker 34 is embedded and installed in the middle of the four sides of the upper end frame of the loading box 27. The end of the knocker 34 extends outward and is fixedly connected to the rubber block 35.

[0030] The automatic transport unit 2 automatically transports the glass test tube 33 containing nucleic acid, buffer solution, fluorescent reagent and corresponding enzyme solution. The transport in and out process can prevent people from directly touching the test tube with their hands. The rubber insert frame 31 is used for inserting the glass test tube 33. The glass test tube 33 is vertically inserted into the insert hole 32, and the bottom of the test tube is extended into the heater 36 for heating. By controlling the heating temperature of the nucleic acid material in the test tube, the unwinding and recombination process of DNA is achieved. The knocker 34 on the side of the container 27 hits the card frame 26 in turn, causing the container 27 and the glass test tube 33 to shake, thereby causing the material in the glass test tube 33 to vibrate, so that the enzyme and the nucleic acid material are in contact more fully, which plays a role in increasing the reaction rate. The rubber block 35 can hit the card frame 26 at the end of the knocker 34 to play a buffering protection role and reduce the noise generated by the collision.

[0031] The automatic transport unit 2 comprises a fitting groove 21, a transport channel 22, a clamping slide 23, a hydraulic cylinder 24, a side slide bar 25 and a start button 29. A longitudinal transport channel 22 is provided inside the installation box 1. A slightly larger fitting groove 21 is provided at the front end of the transport channel 22. A clamping slide 23 is provided on the left and right side walls of the transport channel 22. A side slide bar 25 is slidably connected inside each clamping slide 23. Two left and right hydraulic cylinders 24 are embedded and installed on the rear wall of the transport channel 22. The telescopic end of the hydraulic cylinder 24 faces forward horizontally and is fixedly connected to the tail of the side slide bar 25. The hydraulic pump of the hydraulic cylinder 24 is electrically connected to the output end of the external power supply through the start button 29 installed on the right front side of the installation box 1. The start button 29 is used to control the hydraulic cylinder 24 to work. The hydraulic cylinder 24 drives the clamping frame 26 to move through the side slide bars 25 on both sides, thereby driving the loading box 27 to move forward and backward along the transport channel 22 to realize the loading and feeding process.

[0032] The automatic transport unit 2 further comprises a card-mounting frame 26, a container 27 and a hand ring 28. The card-mounting frame 26 is fixedly connected between the left and right side slide bars 25. The card-mounting frame 26 is a square frame with an L-shaped cross section. The container 27 is limitedly clamped on the step platform formed by the L-shaped structure of the card-mounting frame 26. A hand ring 28 is fixedly connected to the upper side of both the front and rear ends of the card-mounting frame 26. The card-mounting frame 26 is used to limit the container 27. The container 27 is placed in the card-mounting frame 26 from the upper side. The hand ring 28 can facilitate the loading and unloading process of the container 27.

[0033] The automatic transport unit 2 further comprises a front closing plate 210, a sealing strip 211 and a heat preservation plate 212. The front closing plate 210 is fixedly connected to the front ends of the two side sliding bars 25. The front closing plate 210 corresponds to the fitting groove 21 in the longitudinal direction and has the same size. The heat preservation plate 212 is fixedly installed on the rear side of the front closing plate 210. The heat preservation plate 212 corresponds to the transport channel 22 in the longitudinal direction and has the same size. A circle of sealing strips 211 is fixedly installed near the edge of the rear side of the front closing plate 210. When the side sliding bar 25 drives the clamping frame 26 to be retracted into the transport channel 22, the front closing plate 210 is tightly attached to the fitting groove 21, and the sealing strip 211 plays a good sealing role between the two. The heat preservation plate 212 can further prevent the heat loss in the transport channel 22, thereby maintaining the stability of the heating temperature of the glass test tube 33.

[0034] It also includes a microprocessor 4, a data interface 5 and a photomultiplier tube 6. The microprocessor 4 is installed on the upper side of the interior of the installation box 1. The microprocessor 4 is electrically connected to a touch display screen. The touch display screen is embedded in the inclined end surface of the front upper end of the installation box 1. The data interface 5 is provided on the right side of the microprocessor 4. A square installation plate is provided below the microprocessor 4. The upper end of the installation plate is filled with an array of photomultiplier tubes 6 in a rectangular area. A full-spectrum LED lamp is installed at the lower end of the photomultiplier tube 6. Each photomultiplier tube 6 corresponds vertically to a glass test tube 33, and the input end of the photomultiplier tube 6 is bidirectionally electrically connected to the upper microprocessor 4. The full-spectrum LED lamp emits multiple colors of light to the fluorescent reagent in the glass test tube 33. During the PCR process, the nucleic acid carrying the fluorescent reagent is arranged in order. The photomultiplier tube 6 is used to convert the captured light and shadow information into an electrical signal. The microprocessor 4 analyzes the transmitted electrical signal and displays the analysis results of the gene on the screen. The screen can be touched to control the operation of the embedded analysis program of the microprocessor 4. The data interface 5 is used to connect an external data line to transmit or save the result data of the gene analysis.

[0035] It also includes a mounting short column 7 and a suction cup foot 8. The four corners of the lower end of the mounting box 1 are fixedly connected with a mounting short column 7, and the lower end of the mounting short column 7 is sleeved with a suction cup foot 8. The mounting short column 7 is used to install the suction cup foot 8, and the suction cup foot 8 can be sucked tightly on the placement surface, thereby ensuring the overall stability of the device during operation.

[0036] The working principle of the PCR analysis instrument for gene analysis provided by the utility model is as follows: when using the instrument, first insert the glass test tube 33 containing nucleic acid, buffer, fluorescent reagent and corresponding enzyme solution into the insertion hole 32 opened on the rubber insert frame 31, then lift the containing box 27 through the hand rings 28 on both sides, and put the containing box 27 into the card installation frame 26 from the upper side. The hand ring 28 can facilitate the process of loading and removing the containing box 27; after the placement is completed, the hydraulic cylinder 24 is controlled by the start button 29 on the right side to start When the hydraulic cylinder 24 works, the clamping frame 26 is driven to move through the side sliding rods 25 on both sides, and then the loading box 27 is driven to move forward and backward along the conveying channel 22 to realize the loading and feeding process; when the side sliding rods 25 drive the clamping frame 26 to be retracted into the conveying channel 22, the front closing plate 210 is tightly attached to the fitting groove 21, and the sealing strip 211 plays a good sealing role between the two. The heat preservation plate 212 can further prevent the loss of heat inside the conveying channel 22, thereby maintaining the stability of the heating temperature of the glass test tube 33. When the container 27 and the glass test tube 33 arrive at the designated position inside the transport channel 22, the gene analysis and detection process can be started: the glass test tube 33 is vertically inserted into the insertion hole 32, and the bottom of the test tube is extended into the heater 36 for heating. By controlling the heating temperature of the nucleic acid material in the test tube, the DNA unwinding and recombination process is dominated. The knocker 34 on the side of the container 27 hits the card mounting frame 26 in turn, causing the container 27 and the glass test tube 33 to shake, thereby causing the material in the glass test tube 33 to vibrate, so that the enzyme and the nucleic acid material are in contact more fully, thereby playing a role in increasing the reaction rate; a square mounting plate is arranged below the microprocessor 4, and a rectangular area of ​​photomultiplier tubes 6 are installed in an array at the upper end of the mounting plate, and a full-spectrum LED lamp is installed at the lower end of the photomultiplier tube 6. Each photomultiplier tube 6 corresponds vertically to a glass test tube 33, and the input end of the photomultiplier tube 6 is bidirectionally electrically connected to the upper microprocessor 4. The full-spectrum LED lamp emits a variety of color lights to the fluorescent reagent in the glass test tube 33. During the PCR process, the nucleic acid carrying the fluorescent reagent is arranged in order. The photomultiplier tube 6 is used to convert the captured light and shadow information into an electrical signal. The microprocessor 4 analyzes the transmitted electrical signal and displays the analysis results of the gene on the screen. The screen can be touched to control the operation of the embedded analysis program in the microprocessor 4. The data interface 5 is used to connect an external data line to transmit or save the result data of the gene analysis. In addition, the installation short column 7 is used for the installation of the suction cup foot 8, and the suction cup foot 8 can be sucked tightly on the placement surface to ensure the overall stability of the device during operation.

[0037] It is worth noting that the knocker 34, heater 36, and photomultiplier tube 6 disclosed in the above embodiments are all bidirectionally electrically connected to the control chip inside the microprocessor 4. The microprocessor 4 controls the knocker 34, heater 36, and photomultiplier tube 6, and the start button 29 controls the operation of the hydraulic cylinder 24 using methods commonly used in the prior art.

[0038] The above description is only an embodiment of the present invention, and does not limit the patent scope of the present invention. Any equivalent structure or equivalent process transformation made by using the contents of the specification and drawings of the present invention, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present invention.

Claims

1. A PCR analysis instrument for gene analysis, characterized in that: It comprises an installation box (1), an automatic transport unit (2) and a shaking and mixing unit (3); The installation box (1) is a square box with an inclined end surface at the front side of the upper end; Automatic transport unit (2): installed on the lower side of the installation box (1); The shaking and mixing unit (3) comprises a rubber inserting frame (31), an inserting hole (32), a glass test tube (33), a knocker (34), a rubber block (35) and a heater (36). The loading box (27) contained in the automatic conveying unit (2) is limitedly clamped inside the clamping frame (26). A rubber inserting frame (31) is placed inside the upper end of the loading box (27). The internal filling array of the rubber inserting frame (31) is provided with a plurality of vertical inserting holes (32). The glass test tube (33) passes through the inserting hole (32) and its bottom is inserted into the interior of the heater (36). The heater (36) is fixedly installed on the lower side of the loading box (27). A knocker (34) is embedded and installed in the middle of the four side surfaces of the upper end frame of the loading box (27). The end of the knocker (34) protrudes outward and is fixedly connected to the rubber block (35).

2. The PCR analysis instrument for gene analysis according to claim 1, characterized in that: The automatic transport unit (2) comprises a fitting groove (21), a transport channel (22), a snap-in slide groove (23), a hydraulic cylinder (24), a side slide rod (25) and a start button (29). A longitudinal transport channel (22) is provided inside the installation box (1). A slightly larger fitting groove (21) is provided at the front end of the transport channel (22). A snap-in slide groove (23) is provided on the left and right side walls of the transport channel (22). A side slide rod (25) is slidably connected inside each snap-in slide groove (23). Two left and right hydraulic cylinders (24) are embedded and installed on the rear wall of the transport channel (22). The telescopic end of the hydraulic cylinder (24) faces forward horizontally and is fixedly connected to the tail of the side slide rod (25). The hydraulic pump of the hydraulic cylinder (24) is electrically connected to the output end of an external power supply through a start button (29) installed on the right front side of the installation box (1).

3. The PCR analysis instrument for gene analysis according to claim 1, characterized in that: The automatic transport unit (2) further comprises a card-mounted frame (26), a containing box (27) and a hand-carrying ring (28); the card-mounted frame (26) is fixedly connected between the left and right side sliding rods (25); the card-mounted frame (26) is a square frame body and has an L-shaped cross-section; the containing box (27) is limitedly card-mounted on a stepped platform formed by the L-shaped structure of the card-mounted frame (26); and a hand-carrying ring (28) is fixedly connected to the upper sides of the front and rear ends of the card-mounted frame (26).

4. The PCR analysis instrument for gene analysis according to claim 2, characterized in that: The automatic transport unit (2) further comprises a front closing plate (210), a sealing strip (211) and a heat preservation plate (212); the front closing plate (210) is fixedly connected to the front ends of the two side sliding rods (25); the front closing plate (210) corresponds longitudinally to the fitting groove (21) and has the same size; a heat preservation plate (212) is fixedly installed on the rear side of the front closing plate (210); the heat preservation plate (212) corresponds longitudinally to the transport channel (22) and has the same size; a circle of sealing strip (211) is fixedly installed on the rear side of the front closing plate (210) near the edge.

5. The PCR analysis instrument for gene analysis according to claim 1, characterized in that: The device also comprises a microprocessor (4), a data interface (5) and a photomultiplier tube (6). The microprocessor (4) is installed on the upper side of the interior of the installation box (1). The microprocessor (4) is electrically connected to a touch display screen. The touch display screen is embedded in the inclined end surface of the upper front side of the installation box (1). The data interface (5) is provided on the right side of the microprocessor (4). A square installation plate is provided below the microprocessor (4). A rectangular photomultiplier tube (6) is installed in an array at the upper end of the installation plate. A full-spectrum LED lamp is installed at the lower end of the photomultiplier tube (6). Each photomultiplier tube (6) corresponds vertically to a glass test tube (33), and the input end of the photomultiplier tube (6) is bidirectionally electrically connected to the upper microprocessor (4).

6. The PCR analysis instrument for gene analysis according to claim 1, characterized in that: It also comprises a mounting short column (7) and a suction cup foot seat (8), wherein each of the four corners of the lower end of the mounting box (1) is fixedly connected with a mounting short column (7), and the lower end of the mounting short column (7) is sleeved with a suction cup foot seat (8).

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