Cell culture device for immune cell amplification

Abstract

The utility model relates to the technical field of cell culture, in particular to a cell culture device for immune cell amplification, which comprises a base horizontally arranged, air cushions distributed along four vertex angles are arranged on the lower end face of the base, the upper end faces of the air cushions are adhered to the lower end face of the base through resin glue, a U-shaped frame is arranged on the upper end face of the base, and the U-shaped frame is connected with the air cushions. The lower end face of the U-shaped frame is fixedly assembled with the upper end face of the base through screws, plate grooves distributed at equal intervals in the vertical direction are formed in the two sides of the inner wall of the U-shaped frame, and a partition plate is slidably inserted between the two plate grooves in the same horizontal line and is of a hollow structure. By means of the structure of the U-shaped frame, it can be guaranteed that the frame is stable after the partition plates are inserted and connected in a butt joint mode, the culture dish containing base can be arranged in a layered mode through the partition plate structure, culture dishes can be placed in the culture dish containing base, a plurality of culture dishes can be placed through multi-point distribution, the culture dishes can be stored in a centralized mode, and centralized storage and transfer are facilitated.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, in particular to a cell culture device for immune cell amplification. Background Art

[0002] Immune cells are a type of cell involved in the human immune system. Their main function is to protect the body from pathogens (such as bacteria, viruses, parasites, etc.).

[0003] Immune cells can be divided into many types, including lymphocytes, monocytes, granulocytes, dendritic cells, macrophages, NK cells, etc.

[0004] Immune cell culture is a technique that simulates the in vivo immune response in vitro and is used to study the function, interaction and mechanism of immune response of immune cells. Immune cell culture mainly includes the following steps:

[0005] Cell separation: First, immune cells, such as lymphocytes, monocytes, macrophages, etc., are separated from experimental animals or human tissues. Common separation methods include density gradient centrifugation and immunomagnetic bead method;

[0006] Cell purification: In order to ensure the specificity of the culture, the isolated immune cells need to be purified. Common purification methods include flow cytometry, immunomagnetic bead method, etc.

[0007] Cell culture: Place the purified immune cells in an appropriate culture medium, add appropriate stimulants (such as antigens, cytokines, etc.), and culture them in an appropriate temperature and gas environment. During the culture process, the proliferation, differentiation, activation, etc. of the immune cells can be observed;

[0008] Cell function testing: According to the purpose of the experiment, the cultured immune cells can be tested for functions, such as cytokine testing, cytotoxicity testing, antibody production testing, etc.

[0009] Data analysis: The functions of immune cells and the mechanisms of immune response can be revealed through analysis of experimental data.

[0010] At present, when culturing immune cells, technicians not only need to carry out the culturing work, but also need to detect the cell culture conditions during the culturing. The detection process requires the centralized transfer of the cell culture dishes, and in the detection link, trainees or interns are also required to learn and observe. Therefore, it is necessary to design a cell culture device that is easy to transfer and observe for such problems, so that it can be operated by experimental specialists. Utility Model Content

[0011] The purpose of the utility model is to solve the above-mentioned shortcomings in the prior art and to propose a cell culture device for immune cell amplification.

[0012] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0013] A cell culture device for amplifying immune cells is designed, comprising a base arranged horizontally at the bottom, the lower end surface of the base is provided with air cushions distributed along four vertex corners, and the upper end surface of the air cushion is bonded to the lower end surface of the base by resin glue, the upper end surface of the base is provided with a U-shaped frame, and the lower end surface of the U-shaped frame is fixedly assembled with the upper end surface of the base by screws, both sides of the inner wall of the U-shaped frame are provided with plate grooves equidistantly distributed in the vertical direction, a partition plate is slidably inserted between two plate grooves on the same horizontal line, and the partition plate is a hollow structure, a plurality of culture dish seats are provided on the upper end surface of the partition plate, and the lower end surfaces of the culture dish seats are bonded and fixed to the upper end surface of the partition plate by resin glue, a cover body is fitted on the upper end surface of the base, and the cover body and the U-shaped frame are arranged to surround and cover, a handle is provided at the center position of the upper end of the cover body, and the two end ends of the handle are fixed to the upper end surface of the cover body by screws.

[0014] In detail, the cover body is made of transparent plastic or transparent tempered glass.

[0015] In detail, the upper end surface of the base is provided with symmetrically distributed strip plates, and the lower end surfaces of the strip plates are fixedly assembled with the upper end surface of the base by screws.

[0016] In detail, a strip groove corresponding to the position of the strip plate is opened on the lower end surface of the cover body, and the inner wall of the strip groove is slidably inserted and docked with the outer wall of the strip plate.

[0017] In detail, a horizontally arranged pin groove is provided on the side wall of the strip plate, a bayonet is arranged inside the pin groove, the end surface of the bayonet is an arc surface, and the outer wall of the bayonet is slidably sleeved with the inner wall of the pin groove.

[0018] In detail, a spring is arranged inside the pin groove, and two ends of the spring are respectively fixedly assembled with the inward side of the bayonet and the inner wall surface of the pin groove.

[0019] In detail, a pin hole penetrating through the cover body is provided on the inner wall surface of the strip-shaped groove, and the inner wall surface of the pin hole is slidably sleeved with the outer wall surface of the bayonet pin.

[0020] In detail, an air valve is arranged at one end of the side wall of the cover body, a joint position of the air valve penetrates the interior of the cover body, and the joint position is sealed by bonding with resin glue.

[0021] The design scheme proposed by the utility model has the following beneficial effects during application:

[0022] 1. The U-shaped frame structure can ensure the stability of the frame after the partition plate is inserted and docked. The partition plate structure allows the culture dish storage seat to be arranged in layers, and the culture dishes can be placed in the culture dish storage seat. Through multi-point distribution, multiple culture dishes can be placed, so that the culture dishes can be stored in a centralized manner, which is convenient for centralized storage and transfer.

[0023] 2. By adding a cover structure to the base and using a pin lock structure, the cover can be stably fixed to the base, thereby ensuring dust protection for the stored culture dishes. When carrying, the entire device can be carried by the handle. The cover is made of transparent plastic or transparent glass material, which can facilitate observation of the condition of the culture dish and is convenient for teaching use. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0025] Figure 2 It is a schematic diagram of the overall structure of the utility model in an oblique top view;

[0026] Figure 3 It is a schematic cross-sectional diagram of the overall structure of the utility model;

[0027] Figure 4 It is a top view schematic diagram of the U-shaped frame of the utility model;

[0028] Figure 5 It is a partial enlarged schematic diagram of the latch part of the utility model.

[0029] In the figure: 10, base; 11, air cushion; 12, U-shaped frame; 13, plate groove; 14, partition plate; 15, culture dish storage seat; 16, cover body; 17, handle; 20, strip groove; 21, strip plate; 22, pin groove; 23, bayonet pin; 24, spring; 25, pin hole; 30, air valve. DETAILED DESCRIPTION

[0030] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments.

[0031] Reference Figure 1-5A cell culture device for immune cell amplification includes a base 10 arranged horizontally at the bottom, the lower end surface of the base 10 is provided with air cushions 11 distributed along four vertex angles, and the upper end surface of the air cushion 11 is bonded to the lower end surface of the base 10 by resin glue, the upper end surface of the base 10 is provided with a U-shaped frame 12, and the lower end surface of the U-shaped frame 12 is fixedly assembled with the upper end surface of the base 10 by screws, and both sides of the inner wall of the U-shaped frame 12 are provided with plate grooves 13 distributed equidistantly in the vertical direction, and two plate grooves 13 on the same horizontal line are provided with a U-shaped frame 12. A partition plate 14 is slidably inserted between the plate grooves 13, and the partition plate 14 is a hollow structure. A plurality of culture dish seats 15 are provided on the upper end surface of the partition plate 14, and the lower end surface of the culture dish seats 15 is bonded and fixed to the upper end surface of the partition plate 14 by resin glue. A cover body 16 is attached to the upper end surface of the base 10, and the cover body 16 and the U-shaped frame 12 are surrounded and covered. A handle 17 is provided at the center position of the upper end of the cover body 16, and the two end ends of the handle 17 are fixed to the upper end surface of the cover body 16 by screws.

[0032] It should be further explained that the cover body 16 is made of transparent plastic or transparent tempered glass material, which can facilitate interns or trainees to directly peek at the situation of the culture dish through the cover body 16.

[0033] It should be further explained that the upper end surface of the base 10 is provided with symmetrically distributed strip plates 21 , and the lower end surface of the strip plates 21 is fixedly assembled with the upper end surface of the base 10 by screws.

[0034] It should be further explained that a strip groove 20 corresponding to the position of the strip plate 21 is opened on the lower end surface of the cover body 16, and the inner wall of the strip groove 20 and the outer wall of the strip plate 21 are slidably inserted and docked, so that the cover body 16 and the base 10 can be quickly positioned and docked.

[0035] It should be further explained that a horizontally arranged pin groove 22 is provided on the side wall of the strip plate 21 , a latch 23 is arranged inside the pin groove 22 , the end face of the latch 23 is an arc surface, and the outer wall of the latch 23 is slidably connected with the inner wall of the pin groove 22 .

[0036] It should be further explained that a spring 24 is disposed inside the pin slot 22 , and two ends of the spring 24 are respectively fixedly assembled with the inward side of the latch 23 and the inner wall surface of the pin slot 22 .

[0037] It should be further explained that a pin hole 25 penetrating through the cover body 16 is formed on the inner wall surface of the strip groove 20 , and the inner wall surface of the pin hole 25 is slidably sleeved with the outer wall surface of the locking pin 23 .

[0038] It should be further explained that an air valve 30 is provided at one end of the side wall of the cover body 16. The joint position of the air valve 30 passes through the interior of the cover body 16, and the connection position is sealed by resin glue. The air valve 30 is a one-way valve. When necessary, the enclosed space formed by the cover body 16 and the base 10 can be evacuated by an external vacuum pump to meet the culture environment of different culture dishes.

[0039] Working method: When multiple culture dishes need to be stored together, the culture dishes are first placed in the culture dish storage seat 15 of the partition plate 14 in sequence, and then the partition plate 14 is slid into the plate groove 13 of the U-shaped frame 12, so that multiple groups of culture dishes can be placed in layers, and then the cover body 16 is covered on the base 10 to protect the placed culture dishes from dust.

[0040] When the cover body 16 and the base 10 are placed closely together, the strip plate 21 and the strip groove 20 are slidably inserted and installed, so that the cover body 16 and the base 10 can be stably positioned. When the position of the latch 23 corresponds to the position of the pin hole 25, the latch 23 slides through the pin hole 25 through the reset effect of the spring 24, so that the cover body 16 and the base 10 can be stably fixed, thereby achieving coverage and protection of the culture dish. When transferring, it can be carried by the handle 17.

[0041] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes within the technical scope disclosed by the present invention according to the technical scheme and the utility model concept of the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A cell culture device for immune cell amplification, comprising a base (10) arranged horizontally at the bottom, characterized in that: The lower end surface of the base (10) is provided with an air cushion (11) distributed along four vertex angles, and the upper end surface of the air cushion (11) is bonded to the lower end surface of the base (10) by resin glue, the upper end surface of the base (10) is provided with a U-shaped frame (12), and the lower end surface of the U-shaped frame (12) is fixedly assembled with the upper end surface of the base (10) by screws, and both sides of the inner wall of the U-shaped frame (12) are provided with plate grooves (13) distributed equidistantly in the vertical direction, and a partition plate (14) is slidably inserted between two plate grooves (13) on the same horizontal line, The partition plate (14) is a hollow structure, and the upper end surface of the partition plate (14) is provided with a plurality of culture dish seats (15), and the lower end surface of the culture dish seats (15) is bonded and fixed to the upper end surface of the partition plate (14) by resin glue. The upper end surface of the base (10) is fitted with a cover body (16), and the cover body (16) and the U-shaped frame (12) are arranged to surround and cover, and a handle (17) is provided at the center position of the upper end of the cover body (16), and the two end portions of the handle (17) are fixed to the upper end surface of the cover body (16) by screws.

2. A cell culture device for immune cell expansion according to claim 1, characterized in that: The cover body (16) is made of transparent plastic or transparent tempered glass.

3. A cell culture device for immune cell expansion according to claim 1, characterized in that: The upper end surface of the base (10) is provided with symmetrically distributed strip plates (21), and the lower end surface of the strip plates (21) and the upper end surface of the base (10) are fixedly assembled by screws.

4. A cell culture device for immune cell expansion according to claim 3, characterized in that: The lower end surface of the cover body (16) is provided with a strip groove (20) corresponding to the position of the strip plate (21), and the inner wall of the strip groove (20) and the outer wall of the strip plate (21) are slidably inserted and docked.

5. A cell culture device for immune cell expansion according to claim 4, characterized in that: A horizontally arranged pin groove (22) is provided on the side wall of the strip plate (21), a bayonet pin (23) is arranged inside the pin groove (22), the end surface of the bayonet pin (23) is an arc surface, and the outer wall of the bayonet pin (23) is slidably sleeved with the inner wall of the pin groove (22).

6. A cell culture device for immune cell expansion according to claim 5, characterized in that: A spring (24) is arranged inside the pin groove (22), and two ends of the spring (24) are respectively fixedly assembled with the inward side of the latch pin (23) and the inner wall surface of the pin groove (22).

7. A cell culture device for immune cell expansion according to claim 6, characterized in that: A pin hole (25) penetrating the cover body (16) is provided on the inner wall surface of the strip-shaped groove (20), and the inner wall surface of the pin hole (25) is slidably sleeved with the outer wall surface of the latch pin (23).

8. A cell culture device for immune cell expansion according to claim 1, characterized in that: An air valve (30) is provided at one end of the side wall of the cover body (16); a joint position of the air valve (30) penetrates the interior of the cover body (16), and the joint position is sealed by bonding with resin glue.

Images