Antibody incubation box for western blotting method

Abstract

The utility model discloses an antibody incubation box for a western blot method, which comprises an incubation box body with an opening at the upper end, an incubation box cover covering the upper end of the incubation box body, a group of antibody incubation tubes mounted below the incubation box cover, and a semiconductor refrigerator arranged at the bottom of an inner cavity of the incubation box body, the rotation control assembly is used for controlling the incubation box body to rotate; the antibody incubation tube is formed by processing a heat conduction material, the bottom of the antibody incubation tube is connected with a refrigeration sheet of the semiconductor refrigeration instrument, and the semiconductor refrigeration instrument is used for cooling an antibody solution in the antibody incubation tube. According to the antibody incubation box disclosed by the utility model, the temperature loss in a conduction process is reduced, the temperature and time required by an experiment can be adjusted and monitored in real time, and the condition consistency of multiple repeated experiments is ensured.

Description

Technical Field

[0001] The utility model relates to the technical field of biological experimental equipment, in particular to an antibody incubation box for protein blotting. Background Art

[0002] Primary antibody binding incubation refers to the biological immunochemical binding reaction that occurs when the primary antibody binds to the antigen on the membrane and the secondary antibody binds to the primary antibody in an appropriate antibody dilution ratio and temperature environment, and it is a one-to-one specific binding. This is an antigen-antibody reaction method commonly used in scientific research and experimental testing in the fields of biochemistry, biology, and immunology in medicine. The essential experimental equipment for this experiment is the primary antibody incubation box.

[0003] According to a modular antibody incubation box disclosed in China Patent Authorization Announcement No. CN220105052U. The application discloses a novel antibody incubation box for protein blotting, which relates to the technical field of medical biological experimental devices. It not only has the advantages of improving refrigeration and cooling efficiency, saving antibodies, and simple operation, but also can monitor the liquid content and incubation time in real time, rotate 360° for uniform incubation, and control the consistency of conditions before and after repeated experiments, and reduce the cost of laboratory equipment configuration and the requirements for experimental sites. The antibody incubation box for protein blotting includes a snap-on lid; a refrigeration device connected to the bottom, a knob for adjusting the temperature and time and a display screen on the side of the refrigeration device, a heat dissipation fan below the refrigeration device, and a connecting line extending to the semiconductor refrigeration chip above; the incubation box cavity contains 6 cylindrical inner grooves made of copper, each of which is tightly attached to the bottom of the inner groove, and the inner groove containing the scale is made of plastic material, and the semiconductor refrigeration chip is connected to the semiconductor refrigerator below through a connecting line; the two sides of the incubation box are connected to the electric rotating connecting rod, which is horizontally fixed in the four-sided pyramid bracket, and the lower side of the bracket is connected to an adjustable speed and time display screen. This application is used to improve the performance of the antibody incubation box and optimize the stability of experimental conditions.

[0004] The modular antibody incubation box can cool the air in the internal cavity, and then cool the primary antibody dilution in the cavity. However, this method of cooling by air conduction will cause the conduction temperature effect to drop significantly after two temperature conductions between the air conduction and the plastic layer of the antibody box. In addition, the incubation box cannot monitor the remaining amount of antibodies and adjust the incubation speed, temperature and time in real time, and cannot ensure the consistency of experimental conditions between repeated experiments. In addition, the swing position of the antibody box is not optimized during incubation. Utility Model Content

[0005] The utility model aims to solve the problems of poor temperature conduction effect and poor consistency of experimental conditions in traditional antibody incubation boxes, and to provide an antibody incubation box for protein blotting.

[0006] The purpose of this utility model is achieved by the following technical solutions:

[0007] The utility model discloses an antibody incubation box for protein blotting, comprising an incubation box body with an upper opening, an incubation box cover covering the upper end of the incubation box body, a group of antibody incubation tubes installed under the incubation box cover, and a semiconductor refrigerator arranged at the bottom of the inner cavity of the incubation box body;

[0008] The antibody incubation tube is made of heat-conducting material, and the bottom of the antibody incubation tube is connected to the cooling plate of the semiconductor refrigerator, and the semiconductor refrigerator cools down the antibody solution in the antibody incubation tube.

[0009] Furthermore, the antibody incubation box also includes a rotation control component for controlling the rotation of the incubation box body, and the rotation control component includes a rotating shaft fixed to two opposite side walls of the incubation box body, two oppositely arranged positioning brackets, and a rotating motor arranged on one of the positioning brackets; the outer ends of the two rotating shafts are respectively rotatably connected to the two positioning brackets, and the output shaft of the rotating motor is connected to the rotating shaft on this side, and the rotating motor drives the incubation box body to rotate via the rotating shaft.

[0010] Furthermore, the inner wall of the upper port of the incubation box body is provided with a locking groove matching the shape of the incubation box cover, and the incubation box cover is locked to the upper end of the incubation box body through the locking groove.

[0011] Furthermore, a shift block is provided on the side of the incubation box cover, and a yield groove corresponding to the shift block on the side of the incubation box cover is provided on the upper end portion of the incubation box body, so as to facilitate the separation of the incubation box cover from the incubation box body.

[0012] Furthermore, a group of incubation tube mounting holes are opened on the incubation box cover, and a tube plug whose lower end shape matches the upper port of the antibody incubation tube is fixed in each incubation tube mounting hole, and the lower end face of the tube plug is provided with a belt clamp for clamping the PVDF membrane; the upper end of the antibody incubation tube is plugged into the fixed tube plug on the incubation box cover, and the lower end of the tube plug clamps the PVDF membrane through the belt clamp, so that the PVDF membrane is immersed in the antibody solution in the inner cavity of the antibody incubation tube.

[0013] Furthermore, the side wall of the antibody incubation tube is provided with a visual window with scale for observing the amount of antibody solution added into the antibody incubation tube.

[0014] Furthermore, the incubation box body is provided with a temperature detection device for monitoring the temperature of each antibody incubation tube.

[0015] Furthermore, the rotating motor is a motor with adjustable speed, which is used to control the rotation speed of the incubation box body.

[0016] Beneficial effects:

[0017] The antibody incubation box of the present invention can manually adjust the rotation frequency and rotation time according to the experimental requirements through the electrically adjustable rotating bracket device connected to both sides of the incubation box, and can monitor the data in real time to ensure the consistency of conditions for repeated experiments. In addition, it can avoid the need to configure an additional set of horizontal shaking instruments, and the amplitude is larger, not just staying in the horizontal plane to shake, but 360° full-scale rotation incubation.

[0018] The antibody incubation box of the present invention is connected to the bottom of the antibody incubation tube through the semiconductor refrigeration plate of the semiconductor refrigerator, and the antibody solution in the antibody incubation tube is cooled by heat conduction. The temperature and time required for the experiment can be manually adjusted according to the experimental needs and monitored in real time to ensure the consistency of conditions for repeated experiments.

[0019] In the antibody incubation box of the present invention, the antibody incubation tube can fix the PVDF membrane by a band clamp to prevent it from moving, so as to prevent the strip from flowing with the liquid due to the influence of flow force when the liquid flows, thereby reducing the incubation contact rate; at the same time, the antibody incubation tube is encapsulated by a tube plug to prevent the primary antibody dilution working solution from leaking. In addition, the main body of the antibody incubation tube is made of heat-conducting material to reduce the temperature loss during the conduction process; the graduated visual window on the side wall of the antibody incubation tube can observe the amount of antibody solution added to the antibody incubation tube. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a schematic diagram of the overall structure of the antibody incubation box;

[0021] Figure 2 It is a schematic diagram of the structure of the incubation box cover in the antibody incubation box;

[0022] Figure 3 is a schematic diagram of the structure of the antibody incubation tube in the antibody incubation box;

[0023] In the figure, 1-incubation box cover, 11-dial block, 12-band clamp, 13-tube plug, 2-antibody incubation tube, 21-copper tube body, 22-visual window, 3-incubation box body, 4-semiconductor refrigerator, 5-positioning bracket, 6-rotating shaft, 7-rotating motor. DETAILED DESCRIPTION

[0024] The present invention will be further described below with reference to the accompanying drawings and embodiments:

[0025] Example

[0026] like Figure 1As shown, a novel antibody incubation box for protein blotting of the utility model comprises an incubation box body 3 with an upper opening, an incubation box cover 1 covering the upper end of the incubation box body 3, a group of antibody incubation tubes 2 installed under the incubation box cover 1, a semiconductor refrigerator 4 arranged at the bottom of the inner cavity of the incubation box body 3, and a rotation control component for controlling the rotation of the incubation box body 3;

[0027] The rotation control component includes a rotating shaft 6 fixed to the two opposite side walls of the incubation box body 3, two oppositely arranged positioning brackets 5, and a rotating motor 7 arranged on one of the positioning brackets 5; the outer ends of the two rotating shafts 6 are respectively rotatably connected to the two positioning brackets 5, and the output shaft of the rotating motor 7 is connected to the rotating shaft on this side, and the rotating motor 7 drives the incubation box body 3 to rotate through the rotating shaft.

[0028] The inner wall of the upper end of the incubation box body 3 is provided with a clamping groove matching the shape of the incubation box cover 1, and the incubation box cover 1 is clamped on the upper end of the incubation box body 3 through the clamping groove.

[0029] like Figure 2 As shown, a shift block 11 is provided on the side of the incubation box cover 1, and a make way groove corresponding to the shift block 11 on the side of the incubation box cover 1 is provided on the upper end portion of the incubation box body 3, so as to facilitate the separation of the incubation box cover 1 from the incubation box body 3; a group of incubation tube mounting holes are provided on the incubation box cover 1, and a tube plug 13 whose lower end shape matches the upper end portion of the antibody incubation tube 2 is fixed in each incubation tube mounting hole, and a belt clamp 12 for clamping the PVDF membrane is provided on the lower end face of the tube plug 13; the upper end of the antibody incubation tube 2 is plugged with the fixed tube plug 13 on the incubation box cover 1, and the lower end of the tube plug 13 clamps the PVDF membrane through the belt clamp 12, so that the PVDF membrane is immersed in the antibody solution in the inner cavity of the antibody incubation tube 2.

[0030] like Figure 3 As shown, the antibody incubation tube 2 is a copper tube body 21, and the side wall of the antibody incubation tube 2 is provided with a visual window 22 with scale;

[0031] The incubation box body 3 is provided with a temperature detection device for monitoring the temperature of each antibody incubation tube 2 .

[0032] The rotating motor is a motor with adjustable speed, which is used to control the rotation speed of the incubation box body 3.

[0033] The method of using the antibody incubation box in this embodiment is:

[0034] 1) Pour the antibody solution into the inner wall of each antibody incubation tube 2, and control the amount of the added antibody solution through the visual window with scale on the side wall of the antibody incubation tube 2;

[0035] 2) clamping each of the cut PVDF membranes on the band clamps 12 at the lower ends of each tube plug 13 on the cover 1 of the incubation box;

[0036] 3) The upper ends of the antibody incubation tubes 2 filled with the antibody solution are respectively inserted into the lower ends of the tube plugs 13 on the incubation box cover 1, and the PVDF membranes clamped at the lower ends of the tube plugs 13 are completely immersed in the antibody solution in the antibody incubation tubes 2;

[0037] 4) The incubation box cover 1 with each antibody incubation tube 2 fixed thereon is clamped on the upper end of the incubation box body 3, and the bottom of each antibody incubation tube 2 is in contact with the refrigeration plate of the semiconductor refrigerator 4; the semiconductor refrigerator 4 cools the bottom of the antibody incubation tube 2 through the refrigeration plate, and the antibody incubation tube 2 maintains the desired low temperature environment uniformly through the characteristics of its own heat conductive material;

[0038] 5) The rotating motor 7 drives the incubation box body 3 to rotate at a desired speed via the rotating shaft;

[0039] 6) After the incubation is completed, the incubation box cover 1 is removed from the incubation box body 3 by the shifting block 11, and then each antibody incubation tube 2 is removed, and the PVDF membrane is taken out for subsequent detection.

Claims

1. A Western blotting antibody incubation kit, characterized in that: It comprises an incubation box body with an upper opening, an incubation box cover covering the upper end of the incubation box body, a group of antibody incubation tubes installed under the incubation box cover, and a semiconductor refrigerator arranged at the bottom of the inner cavity of the incubation box body; The antibody incubation tube is made of heat-conducting material, and the bottom of the antibody incubation tube is connected to the cooling plate of the semiconductor refrigerator, and the semiconductor refrigerator cools down the antibody solution in the antibody incubation tube.

2. The antibody incubation kit for Western blotting as claimed in claim 1, characterized in that: It also includes a rotation control component for controlling the rotation of the incubation box body, the rotation control component includes a rotation shaft fixed to two opposite side walls of the incubation box body, two oppositely arranged positioning brackets, and a rotation motor arranged on one of the positioning brackets; the outer ends of the two rotation shafts are respectively rotatably connected to the two positioning brackets, the output shaft of the rotation motor is connected to the rotation shaft on this side, and the rotation motor drives the incubation box body to rotate through the rotation shaft.

3. The antibody incubation kit for Western blotting as claimed in claim 2, characterized in that: The rotating motor is a motor with adjustable speed, which is used to control the rotation speed of the incubation box body.

4. The antibody incubation kit for Western blotting according to any one of claims 1 to 3, characterized in that: The inner wall of the upper end portion of the incubation box body is provided with a clamping groove matching the shape of the incubation box cover, and the incubation box cover is clamped to the upper end of the incubation box body through the clamping groove.

5. The antibody incubation kit for Western blotting according to any one of claims 1 to 3, characterized in that: A shift block is provided on the side of the incubation box cover, and a yield groove corresponding to the shift block on the side of the incubation box cover is provided on the upper end portion of the incubation box body.

6. The antibody incubation kit for Western blotting according to any one of claims 1 to 3, characterized in that: A group of incubation tube mounting holes are provided on the incubation box cover, and a tube plug whose lower end shape matches the upper port of the antibody incubation tube is fixed in each incubation tube mounting hole, and a belt clamp for clamping the PVDF membrane is provided on the lower end face of the tube plug; the upper end of the antibody incubation tube is plugged into the fixed tube plug on the incubation box cover, and the lower end of the tube plug clamps the PVDF membrane through the belt clamp, so that the PVDF membrane is immersed in the antibody solution in the inner cavity of the antibody incubation tube.

7. The antibody incubation kit for Western blotting according to any one of claims 1 to 3, characterized in that: The side wall of the antibody incubation tube is provided with a visual window with scale for observing the amount of the antibody solution added into the antibody incubation tube.

8. The antibody incubation kit for Western blotting according to any one of claims 1 to 3, characterized in that: The incubation box body is provided with a temperature detection device for monitoring the temperature of each antibody incubation tube.

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