Compounds to promote regeneration of bone marrow
a technology of ammonium and compound, which is applied in the field of compounds to promote bone marrow regeneration, can solve the problems of drug candidates not being introduced into clinical practice, further deterioration of physiological conditions, and limiting clinical usefulness
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example 1
Effects of CCcompound1, CCcompound3 and CCcompound26 on Blood Profile in Cyclophosphamide-Treated Mice
[0052]Twelve week old female C57B1 mice were used for the experiments. Each drug was administered intraperitoneally once a day for 5 consecutive days. In the second, third, fourth and fifth groups, mice were administered 120 mg / kg of cyclophosphamide (CP). In a third group, mice were also administered 4.5 mg / kg of CCcompound1. In a fourth group, mice were also administered 4.5 mg / kg of CCcompound3. In a fifth group, mice were also administered 2.0 mg / kg of CCcompound26. In the first group, mice received no treatment. Each group included 5 animals. After 3 hours of the last treatments (on day 5), blood samples were collected from the ocular venous plexus of ether-anesthetized mice by retro-orbital venipuncture in heparanized tubes. Complete cell counts (RBC, red blood cell; WBC, white blood cell; PTL, platelet) were determined with a Sysmex F-800 hematology analyzer (Toa Medical Elec...
example 2
[0054]This experiment was designed to determine whether in a tumor model CC compounds could prevent cyclophosphamide-induced loss of body weight resulting from the depression of blood marrow function. Specified pathogen free (SPF) hygienic category colonies of first generation hybrid BDF1 (C57B1 female×DBA / 2 male) adult female mice, weighing 23-24 g, were used. The animals were kept in macrolon cages at 22-24° C. (45-55% humidity), with a lighting regimen of 12 / 12 h light / dark. The animals had free access to tap water and were fed with a sterilized standard diet (Charles River VRF1, autoclavable, Germany) ad libitum. The animals used in these studies were cared for according to the “Guiding Principles for the Care and Use of Animals” based upon the Helsinki declaration.
[0055]MXT mammary tumor pieces (containing about 106 tumor cells) were implanted subcutaneously to develop the tumors. After six days when the tumor-bearing mice were first treated, the sizes of the tumors were in the...
example 3
CCcompound1 Promotes Recovery of Bone Marrow Function after Sublethal Whole Body Irradiation
[0058]This experiment served to determine if CCcompound1 was able to promote regeneration of partially ablated bone marrow from endogenous stem cells i.e. promote restoration of the capacity of bone marrow to produce blood cells. The CFU-GM assay was performed because the number of CFU-GM per femur is a good indicator of bone marrow regeneration. Ballestrero, A., Ferrando, F., Garuti, A., Basta, P., Gonella, R., Stura, P., Mela, G. S., Sessarego, M, Gobbi, M. and Patrone, F., “Comparative effects of three cytokine regimens after high-dose cyclophosphamide: Granulocyte colony-stimulating factor, granulocyte-macrophage colony-stimulating factor (GM-CSF), and sequential interleukin-3 and GM-CSF,”J. Clin. Oncol., 17, 1296-1303 (1999).
[0059]C57B1 female (10-14 weeks old) mice received sublethal (250 cGy) whole body irradiation from a 137Cesium source (day 0). Starting three days before irradiation...
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