Magnetic holder for immunoelectron microscopy grids

a magnet holder and immunoelectron technology, applied in the field of magnet holder for immunoelectron microscopy grids, can solve the problems of affecting the imaging effect, section contamination, and damage to sections loading on the nickel grid, and achieve the effects of reducing the mechanical damage of the grid, improving the success rate of immunolabeling, and reducing the amount of liquid

Active Publication Date: 2021-07-08
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0010]The beneficial technical effects of the present invention: the uniform transfer of batches of sample-loaded nickel grids in different reaction solutions is realized. Through the present invention, not only can the reaction time of the section in each of the sample-loaded nickel grids be unified, but also greatly reduce the mechanical damage to the grids, and it can also reduce the amount of liquid carried out by nickel grids during the continuous replacement of the nickel net between different liquids to almost zero, which greatly improves the success rate of immunolabeling at the sample preparation level.
of the present invention: the uniform transfer of batches of sample-loaded nickel grids in different reaction solutions is realized. Through the present invention, not only can the reaction time of the section in each of the sample-loaded nickel grids be unified, but also greatly reduce the mechanical damage to the grids, and it can also reduce the amount of liquid carried out by nickel grids during the continuous replacement of the nickel net between different liquids to almost zero, which greatly improves the success rate of immunolabeling at the sample preparation level.

Problems solved by technology

If the tweezers touch the grid inner area of the nickel grid in this process, the tweezers can easily touch sections on the grid or the Formvar membrane or carbon membrane adheres to the surfaces of the grids, thereby directly or indirectly damaging the sections loading on the nickel grid, thus affecting following observation .
In addition, in the process of transferring the nickel grid, there is often residual liquid that was not cleaned in the previous step in the gap of the tweezers and carried into the next reaction solution, which can easily lead to section contamination (i.e. unexpected background) Under high-resolution observation of an electron microscope, these unexpected background are irregularly mixed with the secondary antibody colloidal gold particles of only 6-15 nm in the immunolabeling, which seriously affects the imaging effect.
Furthermore, usually one immunolabeling experiment needs to simultaneously process multiple sets of sections for labeling under different test conditions; and the number of sections processed in batches is large.
The copper grid has a high hardness and can be easily inserted into a wax layer; but a nickel grid has a low hardness, and is difficult to insert it into a wax layer.
In addition, the device is only suitable for a single staining operation.
If the device is used to load a batch of copper grids and continuously transfer the grids in liquid of multiple steps, it will carry too much residual liquid from the previous steps.

Method used

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  • Magnetic holder for immunoelectron microscopy grids
  • Magnetic holder for immunoelectron microscopy grids
  • Magnetic holder for immunoelectron microscopy grids

Examples

Experimental program
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Effect test

example 1

[0016]As shown in FIG. 1, a magnetic holder for nickel grids with a frame located entirely at one side of a magnet comprises the frame 1, the magnet 2 and a hydrophobic layer 3. The frame 1 comprises at least one arm 101 with a hollow interior. The arms 101 are made of a solid material. The distance between these hollow interiors of the arms is 2.6 mm. A plurality of axisymmetric arc-shaped grooves are provided in positions located at an upper end and a lower end of an outer surface of the arms 101 and close to the hollow interiors. The openings of the arc-shaped grooves all face the opposite sides, and the two symmetrical arc-shaped grooves can just accommodate the nickel ring on the outside of one nickel grid. The magnet 2 is a magnetic iron detachably arranged below the frame 1. The hydrophobic layer 3 adheres to the outer surfaces of the frame 1 and the magnet 2.

[0017]Operation: separating the frame 1 and the magnet 2, placing the outer rings of the upper sides and lower sides o...

example 2

[0018]As shown in FIG. 2, a magnetic holder for immunoelectron microscopy grids with a magnet located between two adjacent arms comprises a frame 1, the magnet 2 and a hydrophobic layer 3. The frame 1 comprises at least two axisymmetric arms 101. The distance between the two adjacent arms 101 is 2.6 mm to 3 mm. The thickness of one arm 101 is not less than 0.1 mm. Each of the arms 101 is made of a solid material such as iron, cobalt or nickel, which are easily magnetizable. The hydrophobic layer 3 adheres to the outer surface of each of the arms 101. The magnet 2 is a magnetic iron with a length and a width being both smaller than that of the arms 101. The magnet 2 is located between the two adjacent arms 101, and connects and magnetizes the arms 101. The hydrophobic layer 3 adheres to the outer surface of the magnet 2.

[0019]Operation: placing the outer rings of the upper sides and lower sides of the plurality of nickel grids 4 correspondingly on the two adjacent arms 101 magnetized...

example 3

[0020]As shown in FIG. 3, a magnetic holder for immunoelectron microscopy grids with magnets located inside arms comprises a frame 1, the magnets 2 and a hydrophobic layer 3. The frame 1 comprises at least two axisymmetric arms 101 and a connecting part 102. The overall thickness of one arm 101 is not less than 0.1 mm. The interiors of the arms are hollow, and the exteriors of the arms are made of a solid material. The arms 101 are connected by the connecting part 102. The distance between the two adjacent arms 101 is 2.6 to 3.3 mm. The magnets 2 are magnet iron located in the hollow interiors of the arms 101. The hydrophobic layer 3 adheres to the outer surface of the frame 1.

[0021]Operation: placing the outer rings of the upper sides and lower sides of the plurality of nickel grids 4 correspondingly on the two adjacent arms 101. The nickel grids 4 are fixed by the magnetic force of the magnetic bodies inside the arms 101 so as to achieve the batch immunolabeling operation of the n...

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Abstract

The present invention relates to a magnetic holder for immunoelectron microscopy grids. The holder comprises a frame, a magnet and a hydrophobic layer. The device can use a magnetic force to simultaneously attach the outer rings of nickel grids to the frame, so that a batch operation (such as rinsing, immunolabeling and dyeing) of the nickel grids can be realized. In addition, due to the hydrophobic effect of the hydrophobic layer, the holder can reduce the amount of the liquid carried by the nickel grids in the process of continuously transferring the nickel grids between different types of liquids to almost zero. Compared with the prior art, the magnetic holder effectively reduces the probability of cross-contamination between reagents.

Description

[0001]This application claims the priority of Chinese Patent Application No. 202010015283.2, filed on Jan. 7, 2020, the entire disclosure of which is incorporated herein by reference.TECHNICAL FIELD[0002]The present invention relates to a holder for carrying nickel grids with biological ultrasections used in immunoelectron microscope sample preparation technology, in particular to a holder for carrying the nickel grids with a high throughput during rinsing, antigen-antibody labeling and staining.BACKGROUND ART[0003]A nickel grid is a necessary ultrasections carrier applied to biological sample preparation technology of immunoelectron microscope. The nickel grid comprises two parts: one is a nickel ring with a rim width of about 0.2 mm, and another is hollow grids of different sizes ranging from 50-200 meshes inside the nickel ring. The thickness of the whole nickel grid is about 18 micrometers. There is an organic Formvar layer on the front of the nickel grid. Some laboratories spra...

Claims

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Application Information

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Patent Type & AuthorityApplications(United States)
IPC IPC(8): G01N23/2202G01N23/2204G01N33/532
CPCG01N23/2202G01N23/2204G01N2223/612G01N2223/309G01N33/532G01N1/28
InventorLIANG, JINGNANLIU, YIWEIWANG, QIANJIANG, ZHOUSINUODAI, XINGLIANG, HAOLUO, YUANMINGLI, CHUNLIFAN, ZHENGZHAO, TONGSUN, SHUTAOZHANG, XIAOLAN
OwnerINST OF MICROBIOLOGY - CHINESE ACAD OF SCI