Microcapsule suspension type fluidized bed type bioreactor for artificial liver

Abstract

The invention belongs to the field of medical biotechnology, and is a microcapsule suspension type fluidized bed bioreactor for an artificial liver. The reactor is funnel-shaped with a small bottom diameter and a large top diameter. There is a cell screen 8 on the upper neck, and a cell screen 7 on the lower bottom. A cell filter 11 is set at the liquid outlet 2, and between the liquid inlet 1 and the cell screen 7, There is a small buffer chamber 12 between the cell filter 11 and the cell screen 8, and the microencapsulated hepatocytes 9 are accommodated between the cell screen 8 and the cell screen 7; the liquid inlet is externally connected to a membrane oxygenator 14, and the liquid outlet is externally connected to Immunosorber 15 for adsorbing immune macromolecules. The artificial liver bioreactor provided by the present invention is designed to be funnel-shaped, the bottom diameter of the container is small, the top diameter is large, and the movement track of the flow impact is a gentle parabola, which conforms to the characteristics of fluid mechanics, effectively avoids perfusion dead space and ineffective perfusion, and can Effectively exert the therapeutic effect of bioartificial liver.

Description

technical field

[0001] The invention belongs to the technical field of biomedicine, and relates to a medical device, a core device for biological or mixed biological artificial liver treatment-a microcapsule suspension fluidized bed bioreactor for artificial liver. Background technique

[0002] Bioreactor is the core device of biological and hybrid artificial liver support systems. It is not only a place for material exchange between exogenous liver cells and patient blood or plasma, but also provides an ideal growth environment for liver cells. It is used as an artificial liver. Bioreactors generally meet the following basic requirements: bidirectional transmission of small and medium molecules, and immune barriers of macromolecules to ensure liver cell activity and a certain capacity.

[0003] At present, hollow fiber bioreactors are the most widely used, but the disadvantages of hollow fiber bioreactors are uneven cell distribution and poor cell adhesion. In practical app...

Examples

Embodiment Construction

[0015] The shape of the microcapsule suspension fluidized bed reactor for the artificial liver in this embodiment is funnel-shaped, with a plastic or plexiglass shell. During actual production, it can be enlarged or reduced according to needs, and the required size can be formulated.

[0016] Such as figure 1 , figure 2 Shown: 1 is the inlet of blood / plasma / or culture solution, for blood / plasma / culture solution to enter the fluidized bed reactor container, in which material exchange with microencapsulated hepatocytes takes place, 2 for blood / plasma / or Outlet of the culture solution, 3 is the preflush / microencapsulated hepatocyte inlet, through which the hepatocyte suspension is injected and flushed into the preflush, 4 is the valve, which is closed after the microencapsulated hepatocytes are inoculated, and 5 is the sampling port, For sampling during artificial liver treatment, 6 is the sampling port closing valve, 7 is the cell screen with the bottom diameter of the funnel...

technical field

[0001] The invention belongs to the technical field of biomedicine, and relates to a medical device, a core device for biological or mixed biological artificial liver treatment-a microcapsule suspension fluidized bed bioreactor for artificial liver. Background technique

[0002] Bioreactor is the core device of biological and hybrid artificial liver support systems. It is not only a place for material exchange between exogenous liver cells and patient blood or plasma, but also provides an ideal growth environment for liver cells. It is used as an artificial liver. Bioreactors generally meet the following basic requirements: bidirectional transmission of small and medium molecules, and immune barriers of macromolecules to ensure liver cell activity and a certain capacity.

[0003] At present, hollow fiber bioreactors are the most widely used, but the disadvantages of hollow fiber bioreactors are uneven cell distribution and poor cell adhesion. In practical app...

Examples

Embodiment Construction

[0015] The shape of the microcapsule suspension fluidized bed reactor for the artificial liver in this embodiment is funnel-shaped, with a plastic or plexiglass shell. During actual production, it can be enlarged or reduced according to needs, and the required size can be formulated.

[0016] Such as figure 1 , figure 2 Shown: 1 is the inlet of blood / plasma / or culture solution, for blood / plasma / culture solution to enter the fluidized bed reactor container, in which material exchange with microencapsulated hepatocytes takes place, 2 for blood / plasma / or Outlet of the culture solution, 3 is the preflush / microencapsulated hepatocyte inlet, through which the hepatocyte suspension is injected and flushed into the preflush, 4 is the valve, which is closed after the microencapsulated hepatocytes are inoculated, and 5 is the sampling port, For sampling during artificial liver treatment, 6 is the sampling port closing valve, 7 is the cell screen with the bottom diameter of the funnel...

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