Method for preparing AMF (Arbuscular Mycorrhizal Fungus) capsule fungicide

A technology of arbuscular mycorrhizal fungi and arbuscular mycorrhiza, applied in botany equipment and methods, horticulture, application, etc., can solve the problems of unfavorable fungal growth and reproduction, low bacterial content, affecting the use effect of bacterial agents, etc.

CN103814754AInactive Publication Date: 2014-05-28ZHEJIANG NORMAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2014-05-28
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a method for preparing AMF (Arbuscular Mycorrhizal Fungus) capsule fungicide. The method is characterized in that sodium alga acid is used as a basic carrier, a mixture is obtained by matching and mixing multiple fillers such as chitosan, gelatin, glycerol, rice, peat, coal cinder and malt sprout in the basic carrier, a fungicide mixture can be obtained by embedding the fungicide in the mixture after the mixture is sterilized, a spherical object can be prepared by dripping the fungicide mixture into calcium chloride solution in a physical extrusion way, and the AMF capsule fungicide can be obtained after the spherical object is solidified and cleaned. According to the method disclosed by the invention, spores, mycorrhiza and hypha of inner glomus intraradices fungus are embedded in the sodium alga acid, a complete infection process can be completed when the spores, the mycorrhiza and the hypha are seeded in unpasteurized soil, and the infection rate can be increased with the prolonging of the infection time. The method disclosed by the invention has the advantages of simpleness, low cost and low toxicity; an embedding material of the AMF capsule fungicide prepared by the invention can be easily decomposed by microorganism, the preservation time is longer, and the AMF capsule fungicide can be simultaneously and directly seeded in a big field.
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Description

technical field

[0001] The invention belongs to the technical field of capsule inoculum preparation, and in particular relates to a preparation method of arbuscular mycorrhizal fungus capsule inoculum. Background technique

[0002] Arbuscular mycorrhizal fungus (Arbuscular mycorrhizalfungus, arbuscular mycorrhizal fungus) is an endophytic mycorrhizal fungus widely present in soil, and it can form a symbiotic system with 80% of the plants on the earth. Studies have shown that arbuscular mycorrhizal fungi can improve water metabolism in plants, improve drought and saline-alkali resistance; enhance plant disease resistance; promote plants' absorption and utilization of various mineral elements, thereby promoting growth and improving quality. Arbuscular mycorrhizal fungi, on the other hand, use carbohydrates provided by the host plant for their growth and reproduction. Arbuscular mycorrhizal fungi are obligate symbiotic fungi and cannot synthesize DNA by themselves. Therefore, ...

Examples

Embodiment

[0027] The production of embodiment arbuscular mycorrhizal fungus capsule inoculum

[0028] (1) Preparation of liquid bacterial agent

[0029] Take the solid inoculum of Glomus intraradices (Glomus intraradices) obtained by Ri-TDNA carrot root double culture (the specific preparation method of the inoculum can be found in Publication No. CN101565689A titled: "High-density pure-bred production method of arbuscular mycorrhizal fungus spores "invention patent), put it into a grinder and add a small amount of water to grind it, take it out, wash it repeatedly with sterile water in a 400-mesh sieve, remove the agar medium, and keep the concentration of (3-6) ×10 2 CFU / ml arbuscular mycorrhizal fungus liquid inoculum, containing highly infective mycorrhizae, spores and hyphae.

[0030] (2) Preparation of basic carrier and filler

[0031] Mix the basic carrier sodium alginate and the filler evenly. The mass ratio of the basic carrier to the filler is 2-3:2-9. The type and amount o...