Method for taking 3-aryl-5-methyl butyrolactone compound as acetylglucosyl bromide

Abstract

The invention discloses a method for taking 3-aryl-5-methyl butyrolactone compound as acetylglucosyl bromide, and relates to the acetylglucosyl bromide prepared from a chemical compound. The method comprises a method for resisting the activity of tobacco mosaic viruses, a method for killing the activity of funguses in vitro and a method for killing the activity of the funguses in vivo. The suppression rate of (Ia-1)-(Ia-9) and (Ib-1)-(Ib-9) in the 3-aryl-5-methyl butyrolactone compound to the tobacco mosaic viruses is over 60 percent under the concentration of 500 mu g / mL, and the 3-aryl-5-methyl butyrolactone compound is high in the fungicidal activity to 14 funguses. Compared with the prior art, the method disclosed by the invention has an obvious progress and extremely outstanding economical benefits.

Description

technical field

[0001] The technical scheme of the present invention relates to the anti-plant virus agent of chemical compound, specifically the method that 3-aryl-5-methylbutyrolactone compound is used as anti-plant virus agent. Background technique

[0002] The skeleton structure of butyrolactone widely exists in natural products and drug molecules. 3-(4-methylphenyl)-5-ethyl-2,5-dihydrofuran-2-one (Incrustoporin) was first isolated from the tree basidiomycete Incrustoporia carneola9170 (Zapf, S.; AnkeT. ; Sterner, O.Acta.chem.scand.1995,49,233–234.), biological activity research found that it has certain antibacterial activity and cytotoxicity. The bactericidal activity was evaluated by the agar diffusion method in vitro test mode. The test objects were: Absidia glauca (+) (grey green Absidia), Absidia glauca (–), Aspergillus ochraceus (ochrax), Cladosporium cladosporioides (black mold), Curvularia lunata(Curvularia lunata),Fusarium oxysporum(Fusarium oxysporum),Fusari...

Examples

Embodiment 1

[0036] A. The described 3-aryl-5-methylbutyrolactone compound is a compound having the structure shown in the general formula (I) as shown below:

[0037]

[0038] The compound of structure shown in general formula (I) comprises I again a and I b Two categories, the specific individual compounds are as follows:

[0039]

[0040]

[0041] B. The method that above-mentioned 3-aryl-5-methylbutyrolactone compound is used as anti-plant virus agent is as follows:

[0042] B-1. The steps for anti-tobacco mosaic virus activity are as follows:

[0043] The first step, tobacco mosaic virus purification and concentration determination:

[0044] Tobacco mosaic virus purification and concentration determination were carried out in accordance with the Tobacco Mosaic Virus SOP specification compiled by the Bioassay Laboratory of the Institute of Elements, Nankai University. The virus crude extract was centrifuged twice with polyethylene glycol, then the concentration was measured...

Embodiment 2

[0064] Except the individual compound I of the above-mentioned 3-aryl-5-methylbutyrolactone compound in the second step a In the preparation of -1 medicament solution, weigh the individual compound I of the above-mentioned 3-aryl-5-methylbutyrolactone compound a -140mg is used as the original drug, and then DMF0.4mL is added to the original drug to dissolve, and 1×10 5 μg / mL mother liquor, then dilute with the Tween 80 aqueous solution that mass percent concentration is 1 ‰ to the test concentration except that 100 μg / mL, other are the same as embodiment 1.

Embodiment 3

[0066] Individual compounds other than the above-mentioned 3-aryl-5-methylbutyrolactone compounds are I a -2, other with embodiment 1.

technical field

[0001] The technical scheme of the present invention relates to the anti-plant virus agent of chemical compound, specifically the method that 3-aryl-5-methylbutyrolactone compound is used as anti-plant virus agent. Background technique

[0002] The skeleton structure of butyrolactone widely exists in natural products and drug molecules. 3-(4-methylphenyl)-5-ethyl-2,5-dihydrofuran-2-one (Incrustoporin) was first isolated from the tree basidiomycete Incrustoporia carneola9170 (Zapf, S.; AnkeT. ; Sterner, O.Acta.chem.scand.1995,49,233–234.), biological activity research found that it has certain antibacterial activity and cytotoxicity. The bactericidal activity was evaluated by the agar diffusion method in vitro test mode. The test objects were: Absidia glauca (+) (grey green Absidia), Absidia glauca (–), Aspergillus ochraceus (ochrax), Cladosporium cladosporioides (black mold), Curvularia lunata(Curvularia lunata),Fusarium oxysporum(Fusarium oxysporum),Fusari...

Examples

Embodiment 1

[0036] A. The described 3-aryl-5-methylbutyrolactone compound is a compound having the structure shown in the general formula (I) as shown below:

[0037]

[0038] The compound of structure shown in general formula (I) comprises I again a and I b Two categories, the specific individual compounds are as follows:

[0039]

[0040]

[0041] B. The method that above-mentioned 3-aryl-5-methylbutyrolactone compound is used as anti-plant virus agent is as follows:

[0042] B-1. The steps for anti-tobacco mosaic virus activity are as follows:

[0043] The first step, tobacco mosaic virus purification and concentration determination:

[0044] Tobacco mosaic virus purification and concentration determination were carried out in accordance with the Tobacco Mosaic Virus SOP specification compiled by the Bioassay Laboratory of the Institute of Elements, Nankai University. The virus crude extract was centrifuged twice with polyethylene glycol, then the concentration was measured...

Embodiment 2

[0064] Except the individual compound I of the above-mentioned 3-aryl-5-methylbutyrolactone compound in the second step a In the preparation of -1 medicament solution, weigh the individual compound I of the above-mentioned 3-aryl-5-methylbutyrolactone compound a -140mg is used as the original drug, and then DMF0.4mL is added to the original drug to dissolve, and 1×10 5 μg / mL mother liquor, then dilute with the Tween 80 aqueous solution that mass percent concentration is 1 ‰ to the test concentration except that 100 μg / mL, other are the same as embodiment 1.

Embodiment 3

[0066] Individual compounds other than the above-mentioned 3-aryl-5-methylbutyrolactone compounds are I a -2, other with embodiment 1.

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