A platelet preservation solution

Abstract

The invention provides blood platelet preserving fluid. A preparation method of the blood platelet preserving fluid comprises the following steps: preparing dimethyl sulfoxide, namely DMSO and fresh injection water into a solution with the volume concentration of 100%-50%; agitating uniformly and adding medical carbon; decarbonizing the obtained solution by a titanium rod and filtering the solution by a 0.45-micron micro-pore filter membrane; and filtering by a 0.22-micron micro-pore filter membrane to obtain the blood platelet preserving fluid. The preserving fluid can keep the physiological functions of blood platelets at a low temperature and has no toxic and side effects; and the damages of a receptor can be controlled in the lowest range.

Description

technical field

[0001] The invention relates to the field of biomedicine, in particular to a preservation solution for low-temperature preservation of platelets in vitro. Background technique

[0002] Platelets are an important component of blood, participate in the coagulation process of the body, play a normal hemostatic function, and prevent blood loss after injury; at the same time, platelets also participate in inflammation, immunity and support for endothelial integrity. When platelets are stored at 4°C, the activity decreases rapidly, about 40% activity after 6 hours, and only 20% activity after 12 hours. Platelets stored at room temperature tend to lose their hemostatic function, and the room temperature is relatively high, making it difficult to control bacterial contamination. Septic shock caused by transfusion of bacterially contaminated platelets has always been a serious clinical problem. How to protect the activity of platelets, improve their survival rate in ...

Examples

Embodiment 1

[0014] The preparation method of this platelet preservation solution is:

[0015] Dimethyl sulfoxide (DMSO) and fresh water for injection are prepared into a solution with a volume concentration of 50%, then stirred evenly, and medicinal charcoal is added; then the resulting solution is decarburized by filtration through a titanium rod, and then passed through a 0.45um micropore Filter through a filter membrane, and then filter through a 0.22um microporous membrane to obtain the platelet preservation solution. The dosage of medicinal charcoal is to add 2g of medicinal charcoal to every 1000ml platelet preservation solution.

Embodiment 2

[0017] The preparation method of this platelet preservation solution is:

[0018] Dimethyl sulfoxide (DMSO) and fresh water for injection are prepared into a solution with a volume concentration of 85%, then stirred evenly, and medicinal charcoal is added; then the obtained solution is decarburized by filtration through a titanium rod, and then passed through a 0.45um micropore Filter through a filter membrane, and then filter through a 0.22um microporous membrane to obtain the platelet preservation solution. Wherein the amount of medicinal charcoal is to add 10g of medicinal charcoal to every 1000ml of platelet preservation solution.

Embodiment 3

[0020] The platelet preservation solution obtained in Example 1 was stored at -14°C for 3 hours, then at 14°C for 3 hours, and this cycle was repeated once, and finally stored at room temperature.

technical field

[0001] The invention relates to the field of biomedicine, in particular to a preservation solution for low-temperature preservation of platelets in vitro. Background technique

[0002] Platelets are an important component of blood, participate in the coagulation process of the body, play a normal hemostatic function, and prevent blood loss after injury; at the same time, platelets also participate in inflammation, immunity and support for endothelial integrity. When platelets are stored at 4°C, the activity decreases rapidly, about 40% activity after 6 hours, and only 20% activity after 12 hours. Platelets stored at room temperature tend to lose their hemostatic function, and the room temperature is relatively high, making it difficult to control bacterial contamination. Septic shock caused by transfusion of bacterially contaminated platelets has always been a serious clinical problem. How to protect the activity of platelets, improve their survival rate in ...

Examples

Embodiment 1

[0014] The preparation method of this platelet preservation solution is:

[0015] Dimethyl sulfoxide (DMSO) and fresh water for injection are prepared into a solution with a volume concentration of 50%, then stirred evenly, and medicinal charcoal is added; then the resulting solution is decarburized by filtration through a titanium rod, and then passed through a 0.45um micropore Filter through a filter membrane, and then filter through a 0.22um microporous membrane to obtain the platelet preservation solution. The dosage of medicinal charcoal is to add 2g of medicinal charcoal to every 1000ml platelet preservation solution.

Embodiment 2

[0017] The preparation method of this platelet preservation solution is:

[0018] Dimethyl sulfoxide (DMSO) and fresh water for injection are prepared into a solution with a volume concentration of 85%, then stirred evenly, and medicinal charcoal is added; then the obtained solution is decarburized by filtration through a titanium rod, and then passed through a 0.45um micropore Filter through a filter membrane, and then filter through a 0.22um microporous membrane to obtain the platelet preservation solution. Wherein the amount of medicinal charcoal is to add 10g of medicinal charcoal to every 1000ml of platelet preservation solution.

Embodiment 3

[0020] The platelet preservation solution obtained in Example 1 was stored at -14°C for 3 hours, then at 14°C for 3 hours, and this cycle was repeated once, and finally stored at room temperature.

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