Method for fractionating polysaccharide from cistanche

Abstract

The invention relates to a method for fractionating polysaccharide from cistanche. The method includes the following steps of 1), obtaining fleshy stems of the cistanche, smashing the fleshy stems, performing pretreatment with an ethanol solution, adding purified water to obtained filter residues, and performing solid-liquid separation to obtain clear liquid I and the filter residues II; 2), filtering the clear liquid I with a micro-filtration membrane to obtain permeate liquid; 3), sequentially adopting an ultrafiltration membrane capable of intercepting polysaccharide in hundred thousand level of molecular weight, an ultrafiltration membrane capable of intercepting polysaccharide in 10,000 level of molecular weight, an ultrafiltration membrane capable of intercepting polysaccharide in 1,000 level of molecular weight and a nanofiltration membrane capable of intercepting polysaccharide in 100 level of molecular weight to filter the permeate liquid to obtain soluble polysaccharide products different in molecular weight. The polysaccharide different in molecular weight can be rapidly fractionated, the molecular weight of the products is concentrated and identical in activity, and the polysaccharide is non-toxic and harmless; during preparation, less ethanol is used, production energy consumption is low, fewer process is involved, and the period is short.

Description

technical field

[0001] The invention belongs to the technical field of biochemical separation, and in particular relates to a method for fractionating polysaccharides from Cistanche deserticola. Background technique

[0002] Cistanche is a perennial parasitic plant of the genus Cistanche (Cistanche Hoffmg.et Link) in the family Orobanchaceae, obligately parasitic on the roots of Haloxylon plants, and there are more than 20 species in the world. There are 4 species and 1 variant in China, mainly distributed in Inner Mongolia, Ningxia, Gansu, Qinghai and Xinjiang. Cistanche was first recorded in "Shen Nong's Materia Medica", and it has been recorded in detail in ancient medical books. It has been listed as a top-grade medicinal material for a long time. It is widely used clinically to treat deficiency of kidney yang, impotence and infertility, deficiency of essence and blood, weakness of muscles and bones, soreness of the waist and knees, dryness of the intestines and constip...

Examples

Embodiment 1

[0061] The present embodiment adopts the following method to carry out the fractionation and separation of polysaccharides in Cistanche deserticola:

[0062] Take 10kg of Desert Cistanche, crush it through a 40-mesh sieve, add 150L of ethanol solution with a volume fraction of 80%, extract at 60°C for 60min, filter the extract with a 500-mesh filter cloth, and air-dry the filter residue. Put the dried cistanche filter residue in 200L of purified water, extract at 60°C for 60min, filter the extract with a 500-mesh filter cloth, add 80L of purified water to the filter residue for the first extraction at 80°C for 90min, and filter the extract with a 500-mesh filter cloth, Add 50L of purified water to the filter residue and extract at 90°C for 120min, filter the extract with a 500-mesh filter cloth, and combine about 270L of the clear liquid obtained from three extractions and filtration. The supernatant liquid obtained by filtering the ceramic microfiltration membrane with a pore...

Embodiment 2

[0067] The present embodiment adopts the following method to carry out the fractionation and separation of polysaccharides in Cistanche deserticola:

[0068] Take 10kg of Desert Cistanche, crush it through a 60-mesh sieve, add 150L of ethanol solution with a volume fraction of 95%, extract at 50°C for 90min, filter the extract with a 300-mesh filter cloth, and air-dry the filter residue. Put the dried cistanche filter residue in 150L of purified water, extract at 70°C for 60min, filter the extract with a 500-mesh filter cloth, add 100L of purified water to the filter residue for the first extraction at 90°C for 60min, and filter the extract with a 300-mesh filter cloth, Add 50L of purified water at 90°C to the filter residue and extract for 90 minutes, filter the extract with a 500-mesh filter cloth, and combine the clear liquid obtained from the three extractions and filtration into about 250L. The supernatant liquid obtained by filtering the spiral-wound membrane with a pore...

Embodiment 3

[0073] The present embodiment adopts the following method to carry out the fractionation and separation of polysaccharides in Cistanche deserticola:

[0074] Take 10kg of Desert Cistanche, crush it through a 20-mesh sieve, add 100L of ethanol solution with a volume fraction of 40%, extract at 20°C for 30min, filter the extract with a 500-mesh filter cloth, and dry the filter residue. Put the dried cistanche filter residue in 200L of purified water, extract at 100°C for 60 minutes, filter the extract with a 300-mesh filter cloth, add 100L of purified water to the filter residue for the first extraction at 100°C for 60 minutes, and filter the extract with a 300-mesh filter cloth, Add 50L of purified water to the filter residue and extract at 100°C for 60min, filter the extract with a 500-mesh filter cloth, and combine the clear liquid obtained from three extractions and filtration into about 290L. The supernatant liquid obtained by filtering the ceramic microfiltration membrane ...

technical field

[0001] The invention belongs to the technical field of biochemical separation, and in particular relates to a method for fractionating polysaccharides from Cistanche deserticola. Background technique

[0002] Cistanche is a perennial parasitic plant of the genus Cistanche (Cistanche Hoffmg.et Link) in the family Orobanchaceae, obligately parasitic on the roots of Haloxylon plants, and there are more than 20 species in the world. There are 4 species and 1 variant in China, mainly distributed in Inner Mongolia, Ningxia, Gansu, Qinghai and Xinjiang. Cistanche was first recorded in "Shen Nong's Materia Medica", and it has been recorded in detail in ancient medical books. It has been listed as a top-grade medicinal material for a long time. It is widely used clinically to treat deficiency of kidney yang, impotence and infertility, deficiency of essence and blood, weakness of muscles and bones, soreness of the waist and knees, dryness of the intestines and constip...

Examples

Embodiment 1

[0061] The present embodiment adopts the following method to carry out the fractionation and separation of polysaccharides in Cistanche deserticola:

[0062] Take 10kg of Desert Cistanche, crush it through a 40-mesh sieve, add 150L of ethanol solution with a volume fraction of 80%, extract at 60°C for 60min, filter the extract with a 500-mesh filter cloth, and air-dry the filter residue. Put the dried cistanche filter residue in 200L of purified water, extract at 60°C for 60min, filter the extract with a 500-mesh filter cloth, add 80L of purified water to the filter residue for the first extraction at 80°C for 90min, and filter the extract with a 500-mesh filter cloth, Add 50L of purified water to the filter residue and extract at 90°C for 120min, filter the extract with a 500-mesh filter cloth, and combine about 270L of the clear liquid obtained from three extractions and filtration. The supernatant liquid obtained by filtering the ceramic microfiltration membrane with a pore...

Embodiment 2

[0067] The present embodiment adopts the following method to carry out the fractionation and separation of polysaccharides in Cistanche deserticola:

[0068] Take 10kg of Desert Cistanche, crush it through a 60-mesh sieve, add 150L of ethanol solution with a volume fraction of 95%, extract at 50°C for 90min, filter the extract with a 300-mesh filter cloth, and air-dry the filter residue. Put the dried cistanche filter residue in 150L of purified water, extract at 70°C for 60min, filter the extract with a 500-mesh filter cloth, add 100L of purified water to the filter residue for the first extraction at 90°C for 60min, and filter the extract with a 300-mesh filter cloth, Add 50L of purified water at 90°C to the filter residue and extract for 90 minutes, filter the extract with a 500-mesh filter cloth, and combine the clear liquid obtained from the three extractions and filtration into about 250L. The supernatant liquid obtained by filtering the spiral-wound membrane with a pore...

Embodiment 3

[0073] The present embodiment adopts the following method to carry out the fractionation and separation of polysaccharides in Cistanche deserticola:

[0074] Take 10kg of Desert Cistanche, crush it through a 20-mesh sieve, add 100L of ethanol solution with a volume fraction of 40%, extract at 20°C for 30min, filter the extract with a 500-mesh filter cloth, and dry the filter residue. Put the dried cistanche filter residue in 200L of purified water, extract at 100°C for 60 minutes, filter the extract with a 300-mesh filter cloth, add 100L of purified water to the filter residue for the first extraction at 100°C for 60 minutes, and filter the extract with a 300-mesh filter cloth, Add 50L of purified water to the filter residue and extract at 100°C for 60min, filter the extract with a 500-mesh filter cloth, and combine the clear liquid obtained from three extractions and filtration into about 290L. The supernatant liquid obtained by filtering the ceramic microfiltration membrane ...

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