Tissue culture rapid propagation method for illigera trifoliata

A technology of tissue culture and clover, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve problems such as scientific research and introduction and cultivation difficulties, blackened and rotten fruits, and irregular emergence of seedlings, etc., to improve transplanting Survival rate, good rooting condition, good leaf expansion effect

CN107278903AActive Publication Date: 2017-10-24黄小燕
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2017-10-24

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Abstract

The invention discloses a tissue culture rapid propagation method for illigera trifoliata. The tissue culture rapid propagation method for illigera trifoliate comprises the following steps: (1) taking a stem with a bud of the illigera trifoliate as an explant, inoculating the stem with the bud into a propagation and rejuvenation integrated culture medium, culturing in a culture room for 15 days under the indoor conditions of 22-26 DEG C, 1,400 lux and 8 h / d, and then continuing culture under the natural illumination conditions of 25-28 DEG C and 3,500-4,500 lux for 15 days to obtain cluster buds; (2) inoculating the cluster buds cultured in the step (1) in a rooting and seedling hardening-off synchronization culture medium, culturing the cluster buds for 7 days under the indoor conditions of 22-26 DEG C, 1,500 lux and 10 h / d, and then culturing for 25 days in a greenhouse under the natural light, wherein the temperature is 23-28 DEG C, the light shading degree is 20-40%, and the relative humidity is 60-80%; and (3) transplanting. By the method, the production period of seedlings can be shortened remarkably, the production cost of the seedlings is reduced, the survival rate of the seedlings is increased and the quality of the seedlings is improved.
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Description

technical field

[0001] The invention relates to the technical field of propagation of Ivy clover. More specifically, the present invention relates to a method for rapid propagation of Ivy three-leaf tissue culture. Background technique

[0002] Illigera trifoliata (Griff.) Dunn, also known as safflower ivy, hairy ivy, and three sisters vine, is a plant of the genus Ivy in the family Lilyaceae. Ivy clover is mainly distributed in Guangxi, Guangdong, Yunnan, Sichuan, Hunan, Fujian, Guizhou and other places. The aboveground part is used as medicine, which has the effects of dispelling wind and blood stasis, reducing swelling and relieving pain. Zhouyan, rheumatic bone pain, rheumatism and rheumatoid diseases, polio, etc., the plant cane is the main raw material for the production of Chinese patent medicine "Huatuo Fengtongbao" and its various dosage forms. Recent studies have shown that the alcohol extract of Ivy clover is less toxic and has significant anti-inflammatory effe...

Examples

Embodiment 1

[0025] A tissue culture rapid propagation method of Ivy clover, comprising the following steps:

[0026] 1) Integrated cultivation of reproduction and rejuvenation: use the budded stem section of Ivy clover as explants, inoculate in the integrated medium for reproduction and rejuvenation, and place in the cultivation room for 15 days. The temperature of the cultivation room is 22-26°C, the light intensity is 1400lux, and the The time is 8h / d, and then transferred to the culture temperature of 25-28°C and the natural light intensity of 3500lux to continue the culture for 15 days to obtain robust clustered buds. The formula of the integrated medium for reproduction and rejuvenation is: MS+1.0mg / L BA +0.3mg / L LFS+0.1mg / L IBA+0.1mg / L NAA;

[0027] 2) Synchronized cultivation of rooting and hardening seedlings: step 1) inoculate the clustered buds obtained from the cultivation into the synchronous medium for rooting and hardening seedlings, and cultivate them at an indoor temperatu...

Embodiment 2

[0032] A tissue culture rapid propagation method of Ivy clover, comprising the following steps:

[0033] 1) Integrated cultivation of reproduction and rejuvenation: use the budded stem section of Ivy clover as explants, inoculate in the integrated medium for reproduction and rejuvenation, and place in the cultivation room for 15 days. The temperature of the cultivation room is 22-26°C, the light intensity is 1400lux, and the The time is 8h / d, and then transferred to the culture temperature of 25-28°C and the natural light intensity of 4500lux to continue the culture for 15 days to obtain robust clustered buds. The formula of the integrated medium for reproduction and rejuvenation is: MS+2.0mg / L BA +0.5mg / L LFS+0.3mg / L IBA+1.0mg / L NAA;

[0034] 2) Synchronized cultivation of rooting and hardening seedlings: step 1) inoculate the clustered buds obtained from the cultivation into the synchronous medium for rooting and hardening seedlings, and cultivate them at an indoor temperatu...

Embodiment 3

[0039] A tissue culture rapid propagation method of Ivy clover, comprising the following steps:

[0040] 1) Integrated cultivation of reproduction and rejuvenation: use the budded stem section of Ivy clover as explants, inoculate in the integrated medium for reproduction and rejuvenation, and place in the cultivation room for 15 days. The temperature of the cultivation room is 22-26°C, the light intensity is 1400lux, and the The time is 8h / d, and then transferred to the culture temperature of 25-28°C and the natural light intensity of 4000lux to continue the culture for 15 days to obtain robust clustered buds. The formula of the integrated medium for reproduction and rejuvenation is: MS+1.5mg / L BA +0.4mg / L LFS+0.2mg / L IBA+0.5mg / L NAA;

[0041] 2) Synchronized cultivation of rooting and hardening seedlings: step 1) inoculate the clustered buds obtained from the cultivation into the synchronous medium for rooting and hardening seedlings, and cultivate them at an indoor temperatu...