A method for evaluating the malignancy of cervical squamous cell carcinoma based on the positive expression of sufu protein

A technology of cervical squamous cell carcinoma and protein, which is applied in the field of evaluation of the malignancy of cervical squamous cell carcinoma, can solve problems such as unclear mechanism, and achieve the effect of simple and easy-to-operate evaluation method

CN107782903BActive Publication Date: 2020-02-04江西省妇幼保健院
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2020-02-04

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Abstract

The invention discloses a method for evaluating the malignancy of cervical squamous cell carcinoma through the positive expression of Sufu protein. The cervical squamous cell carcinoma tissue is made into a tissue chip, and immunohistochemical experiments are carried out, and the pathologist's immune scoring is carried out. Grouping is performed according to the immune scoring. According to the staining of Sufu protein, the patients were divided into Sufu protein positive expression group and negative expression group, and statistical methods were used to analyze the clinical characteristics of the two groups of patients. The statistical results of clinical data show that patients in the Sufu protein positive expression group are more likely to have vascular tumor embolism (p=0.046) and interstitial invasion depth ≥ 1 / 2 (p=0.041), and the high expression of Sufu protein is associated with the clinical stage of the patient ( p=0.008) increased significantly. The results show that the positive expression of Sufu protein can directly determine the malignant degree and clinical features of cervical squamous cell carcinoma. This evaluation method is simple and provides a new method and marker for evaluating cervical squamous cell carcinoma.
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Description

technical field

[0001] The invention relates to a method for evaluating the malignancy of cervical squamous cell carcinoma, in particular to a method for evaluating the malignancy of cervical squamous cell carcinoma through the positive expression of Sufu protein. The invention belongs to the technical field of cervical squamous cell carcinoma prognosis. Background technique

[0002] Cervical cancer is one of the most common gynecological tumors. Worldwide, there are 530,000 new cases and 275,000 deaths every year. In developing countries, effective screening, evaluation and treatment can greatly reduce morbidity and mortality. From a histological point of view, the progression of normal cervical epithelium to cervical squamous cell carcinoma (CSCC) requires a series of precancerous lesions, including the evolution from low-grade squamous intraepithelial lesions (LSILs) to high-grade squamous intraepithelial lesions. Intraepithelial lesions (HSILs). Therefore, one of the ...

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Embodiment Construction

[0047]The present invention will be further described below in conjunction with the accompanying drawings and embodiments. It should be noted that the following description is only for explaining the present invention and not limiting its content.

[0048] Used main reagent and experimental instrument in the embodiment are as follows:

[0049] (1) Reagents:

[0050] Paraffin (Sigma A6330, USA);

[0051] Hematoxylin (Sigma, USA);

[0052] Eosin (Sigma, USA);

[0053] Citrate antigen repair solution (Maixin, China);

[0054] Normal goat serum for blocking (Zhongshan Jinqiao, China);

[0055] Antibody diluent (Cell signaling Technology, USA);

[0056] Rabbit hypersensitive two-step immunohistochemical detection kit (Zhongshan Jinqiao, China);

[0057] Concentrated DAB kit (Zhongshan Jinqiao, China);

[0058] Antibody: rabbit anti-Sufu IgG (Epitomics, USA).

[0059] (2) Main instruments:

[0060] Paraffin microtome (Leica, Germany);

[0061] IX-71 fluorescent inverted mi...