A kind of composite bone cement, preparation method and application thereof and a kind of bone repair material

Abstract

The invention discloses a composite bone cement, a preparation method and its application, and a bone repair material. The composite bone cement consists of 50% to 80% of solid phase powder and 20% to 50% of solidified liquid according to the mass percentage. It is prepared by mixing raw materials, the solid phase powder includes phosphate and / or phosphate acid salt, and the solidified liquid includes chitosan, hyaluronic acid, organic acid and water. The composite bone cement of the present invention has good injectability, biological activity, osteoconduction and repair ability, and the bone repair material obtained by curing has good mechanical properties, and its surface is easy for cells to adhere, and at the same time, it can not affect the differentiation ability of cells , the composite bone cement of the invention can be used for filling and repairing bone defects, and as a drug carrier for treating orthopedic related diseases.

Description

technical field

[0001] The invention relates to the field of biomaterials, in particular to a composite bone cement, a preparation method and application thereof, and a bone repair material. Background technique

[0002] Bone cement is a medical material widely used in orthopedic surgery. Bone cement is usually composed of solid powder and curing liquid. It can be cured by mixing it in a certain proportion at room temperature. After the reaction is over, the joint can be fixed or the bone defect can be repaired. Due to the injectability of bone cement, it can minimize the area of ​​minor surgical wounds, speed up the recovery, and reduce the pain of patients. Therefore, it is often used as a filling material and scaffold material for bone tissue, which plays a supporting role and is an indispensable material for bone tissue applications. a member of Since the advent of bone cement in the early 1960s, the main types of bone cement are polymethyl methacrylate (PMMA) bone cem...

Examples

Embodiment 1

[0043] This embodiment provides a composite bone cement, which is prepared by mixing raw materials including 80% solid phase powder and 20% solidification liquid according to mass percentage, and the solid phase powder is composed of anhydrous calcium hydrogen phosphate: tetracalcium phosphate according to 30%: 70% mass percentage is uniformly mixed, and the solidified liquid is prepared by uniformly mixing citric acid: chitosan: hyaluronic acid: water according to 20%: 20%: 10%: 50% mass percentage.

[0044] This embodiment also provides a preparation method of the above-mentioned composite bone cement. Anhydrous calcium hydrogen phosphate: tetracalcium phosphate is mixed uniformly according to the mass percentage of 30%:70% to prepare a solid phase powder, and citric acid is added to deionized water to form Citric acid solution, then add chitosan and hyaluronic acid in the citric acid solution and mix uniformly to prepare a solidified solution, in the solidified solution, citri...

Embodiment 2

[0047] Take anhydrous calcium hydrogen phosphate: tetracalcium phosphate according to the mass percentage of 70%: 30%, mix uniformly to prepare solid phase powder, take citric acid and add deionized water to form a citric acid solution, and then add chitosan in the citric acid solution It is uniformly mixed with hyaluronic acid to prepare a solidified liquid, and the mass ratio of citric acid: chitosan: hyaluronic acid: water in the solidified liquid is 5%: 5%: 1%: 89%. At room temperature, the solid phase powder and the solidified liquid are respectively according to the mass percentages of 50%: 50%, 60%: 40%, 66.7%: 33.3%, 75%: 25% and 80%: 20% (that is, the mass ratios are respectively 1:1, 1.5:1, 2:1, 3:1 and 4:1) were mixed for 2 minutes to prepare five kinds of uncured composite bone cement.

[0048] Take the above five kinds of uncured composite bone cement, and use a medical syringe to characterize the injectability of the composite bone cement. The specific process is...

Embodiment 3

[0050] Take anhydrous calcium hydrogen phosphate: tetracalcium phosphate according to the mass percentage of 40%: 60% and mix evenly to prepare a solid phase powder, take acetic acid and add deionized water to form an acetic acid solution, and then add chitosan and hyaluronic acid to the acetic acid solution in turn The acids are evenly mixed to prepare a solidified liquid, in which the mass ratio of acetic acid: chitosan: hyaluronic acid: water is 15%: 15%: 5%: 65%. At room temperature, the solid phase powder and the solidification solution were mixed according to the mass percentage of 80%:20% for 1 minute respectively, and an uncured composite bone cement was prepared.

[0051] Experimental group: the above-mentioned uncured composite bone cement was filled into a mold with a diameter of 6 mm and a height of 12 mm, cured at 37 degrees Celsius for 24 hours, demolded and taken out to obtain a bone repair material.

[0052] Control group: take solid-phase powder and deionized ...

technical field

[0001] The invention relates to the field of biomaterials, in particular to a composite bone cement, a preparation method and application thereof, and a bone repair material. Background technique

[0002] Bone cement is a medical material widely used in orthopedic surgery. Bone cement is usually composed of solid powder and curing liquid. It can be cured by mixing it in a certain proportion at room temperature. After the reaction is over, the joint can be fixed or the bone defect can be repaired. Due to the injectability of bone cement, it can minimize the area of ​​minor surgical wounds, speed up the recovery, and reduce the pain of patients. Therefore, it is often used as a filling material and scaffold material for bone tissue, which plays a supporting role and is an indispensable material for bone tissue applications. a member of Since the advent of bone cement in the early 1960s, the main types of bone cement are polymethyl methacrylate (PMMA) bone cem...

Examples

Embodiment 1

[0043] This embodiment provides a composite bone cement, which is prepared by mixing raw materials including 80% solid phase powder and 20% solidification liquid according to mass percentage, and the solid phase powder is composed of anhydrous calcium hydrogen phosphate: tetracalcium phosphate according to 30%: 70% mass percentage is uniformly mixed, and the solidified liquid is prepared by uniformly mixing citric acid: chitosan: hyaluronic acid: water according to 20%: 20%: 10%: 50% mass percentage.

[0044] This embodiment also provides a preparation method of the above-mentioned composite bone cement. Anhydrous calcium hydrogen phosphate: tetracalcium phosphate is mixed uniformly according to the mass percentage of 30%:70% to prepare a solid phase powder, and citric acid is added to deionized water to form Citric acid solution, then add chitosan and hyaluronic acid in the citric acid solution and mix uniformly to prepare a solidified solution, in the solidified solution, citri...

Embodiment 2

[0047] Take anhydrous calcium hydrogen phosphate: tetracalcium phosphate according to the mass percentage of 70%: 30%, mix uniformly to prepare solid phase powder, take citric acid and add deionized water to form a citric acid solution, and then add chitosan in the citric acid solution It is uniformly mixed with hyaluronic acid to prepare a solidified liquid, and the mass ratio of citric acid: chitosan: hyaluronic acid: water in the solidified liquid is 5%: 5%: 1%: 89%. At room temperature, the solid phase powder and the solidified liquid are respectively according to the mass percentages of 50%: 50%, 60%: 40%, 66.7%: 33.3%, 75%: 25% and 80%: 20% (that is, the mass ratios are respectively 1:1, 1.5:1, 2:1, 3:1 and 4:1) were mixed for 2 minutes to prepare five kinds of uncured composite bone cement.

[0048] Take the above five kinds of uncured composite bone cement, and use a medical syringe to characterize the injectability of the composite bone cement. The specific process is...

Embodiment 3

[0050] Take anhydrous calcium hydrogen phosphate: tetracalcium phosphate according to the mass percentage of 40%: 60% and mix evenly to prepare a solid phase powder, take acetic acid and add deionized water to form an acetic acid solution, and then add chitosan and hyaluronic acid to the acetic acid solution in turn The acids are evenly mixed to prepare a solidified liquid, in which the mass ratio of acetic acid: chitosan: hyaluronic acid: water is 15%: 15%: 5%: 65%. At room temperature, the solid phase powder and the solidification solution were mixed according to the mass percentage of 80%:20% for 1 minute respectively, and an uncured composite bone cement was prepared.

[0051] Experimental group: the above-mentioned uncured composite bone cement was filled into a mold with a diameter of 6 mm and a height of 12 mm, cured at 37 degrees Celsius for 24 hours, demolded and taken out to obtain a bone repair material.

[0052] Control group: take solid-phase powder and deionized ...

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