Method for determining compound in aerosol brown carbon

Abstract

The invention discloses a method for determining a compound in aerosol brown carbon, which belongs to the technical field of analysis chemistry. At present, the chemical composition of the aerosol brown carbon is unknown, and the existing separation analysis method is low in separation rate for nitrogen-containing imidazole compounds in the aerosol brown carbon and inaccurate in qualitation and quantification. By virtue of the steps such as sample collection, pretreatment, instrument analysis, by utilizing a capillary electrophoresis and four-level rod flight time tandem mass spectrometry combined way, the nitrogen-containing imidazole compounds can be accurately qualified and quantified, and the obtained TIC spectrum is clear and accurate, thereby having a more important significance forresearching the brown carbon.

Description

technical field

[0001] The invention belongs to the technical field of analytical chemistry, in particular to a method for measuring compounds in aerosol brown carbon. Background technique

[0002] Atmospheric aerosol brown carbon has a great impact on radiative forcing, regional environmental quality, and global climate change. Aerosol brown carbon is currently considered to be mainly composed of aromatic rings, including oxygen-containing, nitrogen-containing and other large molecular weight substances, or humic acid substances. The main source is primary incomplete combustion or transformation. Primary sources include biomass combustion and motor vehicle emissions; secondary sources include high NO x Aromatic secondary organic pollutants produced under the environment. The chemical composition of the aerosol brown carbon is unknown.

[0003] Capillary electrophoresis, abbreviated as CE in English, is a chromatographic separation analysis method. CE is often used for t...

Examples

Embodiment 1

[0033] Sample Collection: Aerosol brown carbon samples were collected with a Teflon membrane.

[0034] Pre-treatment: Dissolve the sample in methanol, ultrasonically oscillate, extract for 30 minutes, then filter with a 0.45 μm filter membrane to remove impurities, and then use nitrogen to purge the filtrate to concentrate, and the concentrated sample is mixed with methanol-water with a volume ratio of 50:50 The mixed solvent was dissolved to form a 200 μl mixed solution.

[0035] Instrumental analysis: inject the mixed solution into capillary electrophoresis through the injection needle for separation, use methanol-water solution as the mobile phase, control the flow rate of the mobile phase to 0.5ml / h, and then enter the quadrupole-time-of-flight tandem mass spectrometer through the series tube, and compare the mixed solution Qualitative and quantitative analysis of compounds.

Embodiment 2

[0037] Sample Collection: Aerosol brown carbon samples were collected with a quartz membrane.

[0038] Pre-treatment: Dissolve the sample in methanol, ultrasonically oscillate, extract for 25 minutes, then filter with a 0.4 μm filter membrane to remove impurities, and then use nitrogen to purge the filtrate to concentrate, and the concentrated sample is mixed with methanol-water with a volume ratio of 50:50 The mixed solvent was dissolved to form a 190 μl mixed solution.

[0039] Instrumental analysis: The mixed solution is injected into capillary electrophoresis through the injection needle for separation, and then enters the quadrupole-time-of-flight tandem mass spectrometer through the series tube, and the compounds in the mixed solution are qualitatively and quantitatively analyzed.

Embodiment 3

[0041] Sample Collection: Aerosol brown carbon samples were collected with a Teflon membrane.

[0042] Pre-treatment: Dissolve the sample in methanol, ultrasonically oscillate, extract for 35 minutes, then filter with a 0.5 μm filter membrane to remove impurities, and then use nitrogen to sweep the filtrate to concentrate, and the concentrated sample is mixed with methanol-water with a volume ratio of 50:50 The mixed solvent was dissolved to form a 210 μl mixed solution.

[0043] Instrumental analysis: The mixed solution is injected into capillary electrophoresis through the injection needle for separation, and then enters the quadrupole-time-of-flight tandem mass spectrometer through the series tube, and the compounds in the mixed solution are qualitatively and quantitatively analyzed.

technical field

[0001] The invention belongs to the technical field of analytical chemistry, in particular to a method for measuring compounds in aerosol brown carbon. Background technique

[0002] Atmospheric aerosol brown carbon has a great impact on radiative forcing, regional environmental quality, and global climate change. Aerosol brown carbon is currently considered to be mainly composed of aromatic rings, including oxygen-containing, nitrogen-containing and other large molecular weight substances, or humic acid substances. The main source is primary incomplete combustion or transformation. Primary sources include biomass combustion and motor vehicle emissions; secondary sources include high NO x Aromatic secondary organic pollutants produced under the environment. The chemical composition of the aerosol brown carbon is unknown.

[0003] Capillary electrophoresis, abbreviated as CE in English, is a chromatographic separation analysis method. CE is often used for t...

Examples

Embodiment 1

[0033] Sample Collection: Aerosol brown carbon samples were collected with a Teflon membrane.

[0034] Pre-treatment: Dissolve the sample in methanol, ultrasonically oscillate, extract for 30 minutes, then filter with a 0.45 μm filter membrane to remove impurities, and then use nitrogen to purge the filtrate to concentrate, and the concentrated sample is mixed with methanol-water with a volume ratio of 50:50 The mixed solvent was dissolved to form a 200 μl mixed solution.

[0035] Instrumental analysis: inject the mixed solution into capillary electrophoresis through the injection needle for separation, use methanol-water solution as the mobile phase, control the flow rate of the mobile phase to 0.5ml / h, and then enter the quadrupole-time-of-flight tandem mass spectrometer through the series tube, and compare the mixed solution Qualitative and quantitative analysis of compounds.

Embodiment 2

[0037] Sample Collection: Aerosol brown carbon samples were collected with a quartz membrane.

[0038] Pre-treatment: Dissolve the sample in methanol, ultrasonically oscillate, extract for 25 minutes, then filter with a 0.4 μm filter membrane to remove impurities, and then use nitrogen to purge the filtrate to concentrate, and the concentrated sample is mixed with methanol-water with a volume ratio of 50:50 The mixed solvent was dissolved to form a 190 μl mixed solution.

[0039] Instrumental analysis: The mixed solution is injected into capillary electrophoresis through the injection needle for separation, and then enters the quadrupole-time-of-flight tandem mass spectrometer through the series tube, and the compounds in the mixed solution are qualitatively and quantitatively analyzed.

Embodiment 3

[0041] Sample Collection: Aerosol brown carbon samples were collected with a Teflon membrane.

[0042] Pre-treatment: Dissolve the sample in methanol, ultrasonically oscillate, extract for 35 minutes, then filter with a 0.5 μm filter membrane to remove impurities, and then use nitrogen to sweep the filtrate to concentrate, and the concentrated sample is mixed with methanol-water with a volume ratio of 50:50 The mixed solvent was dissolved to form a 210 μl mixed solution.

[0043] Instrumental analysis: The mixed solution is injected into capillary electrophoresis through the injection needle for separation, and then enters the quadrupole-time-of-flight tandem mass spectrometer through the series tube, and the compounds in the mixed solution are qualitatively and quantitatively analyzed.

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