Material culture method for preparing sugarcane wild species chromosome sample

Abstract

The invention discloses a material culture method for preparing a sugarcane wild species chromosome sample and relates to the technical field of biology. The method has the advantages that a special pot-culture pot is designed, and convenience and practicality are achieved while white tender root tips can be collected conveniently to prepare the sugarcane root tip somatic cell chromosome sample; the root tip materials of sugarcane wild plants are cultured by the pot culture manner, bottom breathability and water permeability and organic fertilizer needed by growth are guaranteed, root system growth is promoted, and the wide sugarcane root tip somatic cell chromosome sample can be conveniently prepared and used for cytological researches; by the cytological researches of the sugarcane wildresources, a scientific basis is provided for the further utilization of the wild resources in sugarcane germplasm innovation and variety improvement, and the development of sugarcane planting production industry is promoted.

Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a material cultivation method for preparing sugarcane wild species chromosome samples. Background technique

[0002] Sugarcane (Saccharum spp.) is a tall plant of the genus Saccharum in the Gramineae subfamily Saccharastrae. In the sugarcane subfamily, there are also some genera that are closely related to sugarcane and have important utilization value for sugarcane breeding, such as Erianthus, Miscanthus, Sclerostachya, Narenga And Imperata (Imperata) and so on. The plants of these genera together with Saccharum are collectively referred to as "Saccharum complex". Because these plants have many excellent traits such as strong tillering, strong stress resistance, and barrenness tolerance, they have potential use value for the cultivation of new sugarcane varieties or the improvement of original varieties, and they are constantly being studied and utilized as sugarcane germplasm res...

Examples

Embodiment 1

[0034] Such as Figure 1-2 Shown:

[0035] A material culture method for preparing wild sugarcane chromosome samples, comprising the following steps:

[0036] 1) dig out the sugarcane wild resource clone material from the resource garden, the material has tiller bud points and 3-5 old roots, and cut off all the leaves of the material;

[0037] 2) Fill the liner 2 of the special potted culture pot 1 with impurity-free soil to 1 / 3 of the total volume, plant the sample in the liner 2 and cover with soil to 1 / 2 of the total volume, water thoroughly and cover with transparent Place the plastic film in a greenhouse at about 30°C for cultivation to allow the seedlings to germinate;

[0038] 3) When the material grows 8-12 tillers and the height reaches 75-85cm, lift the liner 2 together with the soil and the material plant and take it out from the potted culture pot 1;

[0039] 4) Fill the potted culture pot 1 with 1:3 mixed perlite and sun-dried lawn grass to 1 / 3-1 / 2 of the volum...

Embodiment 2

[0046] Such as Figure 1-2 Shown: a potted culture pot for preparing wild sugarcane chromosome sample materials, including a potted culture pot 1 and an inner tank 2, the potted culture pot 1 is a cylindrical pot body, the lower end is equipped with a base 6 and has a through hole, and is placed side by side Place the liner 2 in the potted culture pot 1 on the pot mat 3, the inner pot 2 is a hollow cylinder with a diameter 1-2 cm smaller than the potted culture pot 1, a cross-shaped support 4 and a grid cloth 5 are installed at the bottom, and the upper end is open And install a handle that is symmetrical to the center;

[0047] The potted culture pot 1 and the inner wall of the liner 2 are all designed with a scale line 7;

[0048] The potted culture pot 1 is a pottery pot, and the liner 2 is made of plastic with a thickness of 0.3-0.5 cm.

Embodiment 3

[0050] Such as Figure 1-2 Shown: the application of a special pot culture pot for preparing chromosome sample material of wild sugarcane species in the cultivation of somatic cell chromosome material of wild sugarcane root tip, including:

[0051] After placing the liner 2 in the potted culture pot 1, fill 1 / 3 of the impurity-free soil in the liner 2, plant the prepared sample on the soil and cover the soil to 1 / 2 of the total volume, water thoroughly and cover After the transparent plastic film is placed in a greenhouse at about 30°C, the seedlings will germinate;

[0052] When the material grows 8-12 tillers and the height reaches 75-85cm, the inner container is taken out from the potted culture pot 1 through the handle on the inner container 2, and the soil and material plants in the inner container 2 are completely preserved;

[0053] Fill the potted culture pot 1 with 1:3 mixed perlite and dried lawn grass to 1 / 3-1 / 2 of the volume of the pot, and replant the liner 2 of ...

technical field

[0001] The invention relates to the field of biotechnology, in particular to a material cultivation method for preparing sugarcane wild species chromosome samples. Background technique

[0002] Sugarcane (Saccharum spp.) is a tall plant of the genus Saccharum in the Gramineae subfamily Saccharastrae. In the sugarcane subfamily, there are also some genera that are closely related to sugarcane and have important utilization value for sugarcane breeding, such as Erianthus, Miscanthus, Sclerostachya, Narenga And Imperata (Imperata) and so on. The plants of these genera together with Saccharum are collectively referred to as "Saccharum complex". Because these plants have many excellent traits such as strong tillering, strong stress resistance, and barrenness tolerance, they have potential use value for the cultivation of new sugarcane varieties or the improvement of original varieties, and they are constantly being studied and utilized as sugarcane germplasm res...

Examples

Embodiment 1

[0034] Such as Figure 1-2 Shown:

[0035] A material culture method for preparing wild sugarcane chromosome samples, comprising the following steps:

[0036] 1) dig out the sugarcane wild resource clone material from the resource garden, the material has tiller bud points and 3-5 old roots, and cut off all the leaves of the material;

[0037] 2) Fill the liner 2 of the special potted culture pot 1 with impurity-free soil to 1 / 3 of the total volume, plant the sample in the liner 2 and cover with soil to 1 / 2 of the total volume, water thoroughly and cover with transparent Place the plastic film in a greenhouse at about 30°C for cultivation to allow the seedlings to germinate;

[0038] 3) When the material grows 8-12 tillers and the height reaches 75-85cm, lift the liner 2 together with the soil and the material plant and take it out from the potted culture pot 1;

[0039] 4) Fill the potted culture pot 1 with 1:3 mixed perlite and sun-dried lawn grass to 1 / 3-1 / 2 of the volum...

Embodiment 2

[0046] Such as Figure 1-2 Shown: a potted culture pot for preparing wild sugarcane chromosome sample materials, including a potted culture pot 1 and an inner tank 2, the potted culture pot 1 is a cylindrical pot body, the lower end is equipped with a base 6 and has a through hole, and is placed side by side Place the liner 2 in the potted culture pot 1 on the pot mat 3, the inner pot 2 is a hollow cylinder with a diameter 1-2 cm smaller than the potted culture pot 1, a cross-shaped support 4 and a grid cloth 5 are installed at the bottom, and the upper end is open And install a handle that is symmetrical to the center;

[0047] The potted culture pot 1 and the inner wall of the liner 2 are all designed with a scale line 7;

[0048] The potted culture pot 1 is a pottery pot, and the liner 2 is made of plastic with a thickness of 0.3-0.5 cm.

Embodiment 3

[0050] Such as Figure 1-2 Shown: the application of a special pot culture pot for preparing chromosome sample material of wild sugarcane species in the cultivation of somatic cell chromosome material of wild sugarcane root tip, including:

[0051] After placing the liner 2 in the potted culture pot 1, fill 1 / 3 of the impurity-free soil in the liner 2, plant the prepared sample on the soil and cover the soil to 1 / 2 of the total volume, water thoroughly and cover After the transparent plastic film is placed in a greenhouse at about 30°C, the seedlings will germinate;

[0052] When the material grows 8-12 tillers and the height reaches 75-85cm, the inner container is taken out from the potted culture pot 1 through the handle on the inner container 2, and the soil and material plants in the inner container 2 are completely preserved;

[0053] Fill the potted culture pot 1 with 1:3 mixed perlite and dried lawn grass to 1 / 3-1 / 2 of the volume of the pot, and replant the liner 2 of ...

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