Method for rapidly, efficiently and nondestructively identifying high-lysine waxy corn kernels

Abstract

The invention relates to a method for rapidly, efficiently and nondestructively identifying high-lysine waxy corn kernels. According to the method for rapidly, efficiently and nondestructively identifying the high-lysine waxy corn kernels, grains are harvested in the specific development period of the waxy corn grains, and the phenotype of the grains is taken as an identification index. When the method is used for identifying the high-lysine waxy corn kernels, the accuracy of identifying the high-lysine waxy corn kernels is 90% or above, and the average accuracy is 93%. The method overcomes the defects that the corn kernels are damaged after chemical sampling detection, and undetected kernels cannot be guaranteed to be high-lysine kernels. According to the method for rapidly, efficiently and nondestructively identifying the high-lysine waxy corn kernels, the step of performing molecular marker-assisted detection on the selfing offspring can further be eliminated.

Description

technical field

[0001] The invention relates to the field of plant breeding, in particular to a method for quickly, efficiently and nondestructively identifying high-lysine waxy corn grains. Background technique

[0002] Waxy corn is one of the main types of fresh corn. It has the advantages of unique flavor and rich nutrition. It is an important agricultural product of fruits and vegetables in my country. With the improvement of consumption level, consumers' demand for high-end, high-quality and high-nutrition agricultural products is increasing. Improving the nutritional quality of waxy corn is the development direction of waxy corn breeding. Lysine is one of the essential amino acids for the human body. It plays a very important role in human development, enhancing immunity, and improving central nervous tissue functions. It is also known as the first essential amino acid and the first limiting amino acid. It is of great significance to carry out high-lysine waxy corn br...

Examples

Embodiment 1

[0033] 1. Hybridization

[0034] Jingnuo 6, the backbone inbred line of waxy maize in my country, was selected and provided by the Maize Research Center of Beijing Academy of Agriculture and Forestry Sciences as the female parent, with a lysine content of 0.33%. The maize germplasm material A containing opaque-2 was used as the male parent, whose genotype was homozygous recessive (o2o2), and the lysine content in the grain was 0.48% (the lysine content in the corn grain ≥ 0.4% means high lysine Acid corn), crossed to obtain the F1 generation.

[0035] 2. Acquisition of waxy corn seeds

[0036] (1) Selfing

[0037] The F1 generation seeds in the above 1 were planted and strictly selfed, and the pollination date was recorded. Harvest at the full maturity stage of the grains to obtain F2 generation seeds.

[0038] (2) Backcross

[0039] The seeds of the F1 generation in the above 1 were planted, the F1 plants were used as the female parent, and Jingnuo 6 was used as the male...

Embodiment 2

[0053] 1. Hybridization

[0054] Jingnuo 6, the backbone inbred line of waxy maize in my country, was selected and provided by the Maize Research Center of Beijing Academy of Agriculture and Forestry Sciences as the female parent, with a lysine content of 0.33%. The maize germplasm material A containing opaque-2 was used as the male parent, whose genotype was homozygous recessive (o2o2), and the lysine content in the grain was 0.48% (the lysine content in the corn grain ≥ 0.4% means high lysine Acid corn), crossed to obtain the F1 generation.

[0055] 2. Acquisition of waxy corn seeds

[0056] The seeds of the F1 generation in the above 1 were planted, the F1 plants were used as the female parent, and Jingnuo 6 was used as the male parent for backcrossing, and the pollination date was recorded. Harvested at the full maturity stage of the grains to obtain BC1F1 generation seeds. According to the phenotype, the opaque seeds are selected for next generation planting.

[0057]...

Embodiment 3

[0059] Example 3 Comparison of germplasm characteristics of high lysine waxy maize at different developmental stages

[0060] 1. Hybridization

[0061] Jingnuo 6, the backbone inbred line of waxy maize in my country, was selected and provided by the Maize Research Center of Beijing Academy of Agriculture and Forestry Sciences as the female parent, with a lysine content of 0.33%. The maize germplasm material A containing opaque-2 was used as the male parent, whose genotype was homozygous recessive (o2o2), and the lysine content in the grain was 0.48% (the lysine content in the corn grain ≥ 0.4% means high lysine Acid corn), crossed to obtain the F1 generation.

[0062] 2. Acquisition of waxy corn seeds

[0063] The seeds of the F1 generation in the above 1 were planted, the F1 plants were used as the female parent, and Jingnuo 6 was used as the male parent for backcrossing, and the pollination date was recorded. Harvested at the full maturity stage of the grains to obtain BC...

technical field

[0001] The invention relates to the field of plant breeding, in particular to a method for quickly, efficiently and nondestructively identifying high-lysine waxy corn grains. Background technique

[0002] Waxy corn is one of the main types of fresh corn. It has the advantages of unique flavor and rich nutrition. It is an important agricultural product of fruits and vegetables in my country. With the improvement of consumption level, consumers' demand for high-end, high-quality and high-nutrition agricultural products is increasing. Improving the nutritional quality of waxy corn is the development direction of waxy corn breeding. Lysine is one of the essential amino acids for the human body. It plays a very important role in human development, enhancing immunity, and improving central nervous tissue functions. It is also known as the first essential amino acid and the first limiting amino acid. It is of great significance to carry out high-lysine waxy corn br...

Examples

Embodiment 1

[0033] 1. Hybridization

[0034] Jingnuo 6, the backbone inbred line of waxy maize in my country, was selected and provided by the Maize Research Center of Beijing Academy of Agriculture and Forestry Sciences as the female parent, with a lysine content of 0.33%. The maize germplasm material A containing opaque-2 was used as the male parent, whose genotype was homozygous recessive (o2o2), and the lysine content in the grain was 0.48% (the lysine content in the corn grain ≥ 0.4% means high lysine Acid corn), crossed to obtain the F1 generation.

[0035] 2. Acquisition of waxy corn seeds

[0036] (1) Selfing

[0037] The F1 generation seeds in the above 1 were planted and strictly selfed, and the pollination date was recorded. Harvest at the full maturity stage of the grains to obtain F2 generation seeds.

[0038] (2) Backcross

[0039] The seeds of the F1 generation in the above 1 were planted, the F1 plants were used as the female parent, and Jingnuo 6 was used as the male...

Embodiment 2

[0053] 1. Hybridization

[0054] Jingnuo 6, the backbone inbred line of waxy maize in my country, was selected and provided by the Maize Research Center of Beijing Academy of Agriculture and Forestry Sciences as the female parent, with a lysine content of 0.33%. The maize germplasm material A containing opaque-2 was used as the male parent, whose genotype was homozygous recessive (o2o2), and the lysine content in the grain was 0.48% (the lysine content in the corn grain ≥ 0.4% means high lysine Acid corn), crossed to obtain the F1 generation.

[0055] 2. Acquisition of waxy corn seeds

[0056] The seeds of the F1 generation in the above 1 were planted, the F1 plants were used as the female parent, and Jingnuo 6 was used as the male parent for backcrossing, and the pollination date was recorded. Harvested at the full maturity stage of the grains to obtain BC1F1 generation seeds. According to the phenotype, the opaque seeds are selected for next generation planting.

[0057]...

Embodiment 3

[0059] Example 3 Comparison of germplasm characteristics of high lysine waxy maize at different developmental stages

[0060] 1. Hybridization

[0061] Jingnuo 6, the backbone inbred line of waxy maize in my country, was selected and provided by the Maize Research Center of Beijing Academy of Agriculture and Forestry Sciences as the female parent, with a lysine content of 0.33%. The maize germplasm material A containing opaque-2 was used as the male parent, whose genotype was homozygous recessive (o2o2), and the lysine content in the grain was 0.48% (the lysine content in the corn grain ≥ 0.4% means high lysine Acid corn), crossed to obtain the F1 generation.

[0062] 2. Acquisition of waxy corn seeds

[0063] The seeds of the F1 generation in the above 1 were planted, the F1 plants were used as the female parent, and Jingnuo 6 was used as the male parent for backcrossing, and the pollination date was recorded. Harvested at the full maturity stage of the grains to obtain BC...

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