A spectrum analysis device

Abstract

The present invention provides a spectral analysis device, which relates to the field of fluorescence excitation and detection technology. The analyzer includes a fuselage, a thermal printer installed on the top of the fuselage, and a fishtail handle installed on the bottom of the fuselage. The side of the fuselage is provided with a detection entrance and an optical fiber interface for inserting an object to be detected; the front of the fishtail handle is provided with a keyboard for information interaction, and the back of the fuselage is provided with a battery box for placing batteries. The spectral analysis device provided by the present invention has a compact structure, is portable and easy to use, can analyze and detect human blood and urine based on the principles of fluorescence immunoassay and immunochromatography technology, and can also use external optical fiber transmission to quickly detect the fluorescence value of a specified area. The spectral analysis device solves the technical problems of the complex mechanical transmission structure and low utilization rate of optical path components of the existing analyzer.

Description

Technical Field

[0001] The invention relates to the technical field of fluorescence excitation and detection, and in particular to a spectrum analysis device. Background Art

[0002] Fluorescence immunoassay (FIA) is a microanalysis method developed in recent years and is one of the most sensitive microanalysis techniques. The molecules of certain substances can absorb energy and emit fluorescence. The method of qualitative or quantitative analysis of substances based on the fluorescence spectrum and fluorescence intensity is called fluorescence analysis. Fluorescence immunoassay (FIA) has the advantages of high sensitivity, strong selectivity, small sample volume and simple method. The lower limit of determination of this method is usually 2 to 4 orders of magnitude higher than that of spectrophotometry, and it is widely used in biochemical analysis.

[0003] Currently, the commonly used fluorescence quantitative detectors are usually large in size and not convenient for portable applications, thus limiting their development in underdeveloped areas with inconvenient transportation. Even if there are some commercial products, they still have the disadvantages of complex structure, poor reliability, low precision, and difficult operation. Therefore, achieving instrument portability and scalability is a problem that needs to be solved in this field. Summary of the invention

[0004] In view of the above problems, the present invention aims to provide a spectral analysis device which is compact, portable and easy to use, can analyze and detect human blood and urine based on the principles of fluorescence immunoassay and immunochromatography technology, and can also use external optical fiber transmission to quickly detect fluorescence values ​​in a specified area. The spectral analysis device solves the technical problems of complex mechanical transmission structure and low utilization rate of optical path components in existing analyzers.

[0005] A spectrum analysis device, characterized in that it comprises a body, a thermal printer installed on the top of the body and a fishtail handle installed on the bottom of the body, wherein: the body is a hollow rectangular parallelepiped structure, a liquid crystal screen for displaying data is arranged on the front of the body, a detection entrance for placing an object to be detected and an optical fiber interface for connecting an optical fiber bundle are arranged on the side of the body; a keyboard for information interaction is arranged on the front of the fishtail handle, and a battery box for placing batteries is arranged on the back of the body;

[0006] It also includes a detection card for inserting into the detection entrance, the detection card includes a detection plate, a test strip placed on the detection plate, and a sample adding pad arranged between the detection plate and the test strip, wherein: the front side of the detection plate is recessed downward to form a concave platform for holding the test strip, a transmission rack is arranged on the back side of the detection plate, one end of the detection plate is erected and then folded inward by 90° to form a suspended platform, the other end of the detection plate is erected to form a support plate with the same height as the suspended platform, the suspended platform is located on the top of the sample adding pad, and a sample adding hole is opened on the suspended platform;

[0007] The detection entrance extends toward the inside of the fuselage to form a detection channel, an observation window is provided at the top of the detection channel, and a transmission notch is provided at the bottom of the detection channel;

[0008] A scanning transmission module for driving the detection card to move is also arranged inside the fuselage, and the scanning transmission module includes a transmission gear, a stepping motor for driving the transmission gear to rotate, and a micro switch for sensing the position of the detection card. The transmission gear is arranged in the transmission gap to drive the detection card to move repeatedly by engaging with the transmission rack, and the micro switch is arranged at the bottom of the detection channel;

[0009] The fuselage is also provided with a light source channel for generating a light source, the light source channel is parallel to the detection channel and includes a coaxially arranged light source and a light source filter located on one side of the light source;

[0010] The fuselage is also provided with a light source feedback channel for receiving feedback fluorescence, the light source feedback channel is perpendicular to the detection channel, and includes a first plano-convex lens, a detection filter, a second plano-convex lens, a grating and a spectrum measuring device which are coaxially arranged, and a dichroic mirror inclined at 45° for light splitting is arranged at the intersection of the axis of the light source feedback channel and the light source channel, wherein: the first plano-convex lens is installed on the top of the observation window, the dichroic mirror is located between the first plano-convex lens and the detection filter, and is located on the same side of the light source as the light source filter, and the spectrum measuring device is used to measure the value of the received feedback light;

[0011] The fuselage also includes a collecting optical fiber, a spectroscope and two sets of coupling components, wherein: the collecting optical fiber includes a vertical incident optical fiber for collecting the light source, a horizontal receiving optical fiber for deriving feedback light, and a collection end for collecting the vertical incident optical fiber and the horizontal receiving optical fiber; a coupling component is arranged between the vertical incident optical fiber and the light source; a coupling component is also arranged between the horizontal receiving optical fiber and the dichroic mirror; and the collection end is installed in the optical fiber interface.

[0012] Furthermore, a roller is installed at the bottom of the detection channel to reduce the friction between the detection channel and the detection card.

[0013] Furthermore, the aggregation end includes a parallel incident optical fiber located in the center and a plurality of parallel receiving optical fibers uniformly surrounding the parallel incident optical fiber.

[0014] Furthermore, the aggregation end also includes a vertical incident optical fiber located in the center and parallel receiving optical fibers uniformly surrounding the outside of the vertical incident optical fiber.

[0015] Furthermore, it also includes an optical fiber bundle detachably connected to the optical fiber interface, one end of the optical fiber bundle is connected to the aggregation end of the collected optical fibers through a connector, and the other end of the optical fiber bundle is connected to the optical fiber probe through a connector.

[0016] Furthermore, the coupling component includes a first convex lens, a narrow-band filter and a second convex lens which are coaxially arranged.

[0017] Furthermore, the test paper is provided with a detection band for capturing the antigen-antibody complex, a control band for enriching the free fluorescent marker, and a QR code for recording the serial number.

[0018] Furthermore, a circuit board is also provided in the fuselage, and the circuit board includes a main control chip, an amplifying and filtering circuit, a spectrum measuring device, a light source driving circuit, and an ID chip, a power supply circuit, an A / D conversion circuit, a timing driving circuit, a synchronous control circuit and a motor driving circuit respectively connected to the main control chip, wherein:

[0019] The main control chip is respectively connected to the LCD screen, keyboard and thermal printer;

[0020] The main control chip obtains the spectrum measuring device information through the A / D conversion circuit and the amplification and filtering circuit in sequence;

[0021] The spectrum measuring device is connected to the timing driving circuit;

[0022] The main control chip drives the light source to work through the synchronous control circuit and the light source driving circuit in sequence;

[0023] The main control chip drives the stepper motor control by clicking the drive circuit;

[0024] The main control chip is connected to the positive and negative electrodes in the battery box through a power circuit.

[0025] A spectral analysis device of the present invention has the following beneficial effects: the spectral analysis device has a compact structure, and adopts a transmission scheme in which the rack on the back of the detection card cooperates with the gear of the rotating shaft of the stepping motor, replacing the motion platform scheme of the transmission, and reducing the components such as the slider, the ruler belt, the guide rail, the test strip slot plate, and the positioning device. At the same time, by designing the shape of the detection plate, on the one hand, the shape of the detection channel can be used to guide the movement of the detection card, and on the other hand, it can be convenient for the user to drop the sample. The spectral analysis device is portable and easy to use, and the analyzer includes a thermal printer, a battery box and an LCD screen, and can continue to be used without an external power supply. The spectral analysis device has strong scalability and an external optical fiber interface. The reaction area outside the analyzer can be directly analyzed through an optical fiber bundle, a connector and an optical fiber probe. At the same time, by reasonably designing the optical path, a shared light source is used. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0027] Figure 1 It is a front structural schematic diagram of the present invention;

[0028] Figure 2 It is a side structural schematic diagram of the present invention;

[0029] Figure 3 It is a schematic diagram of the back structure of the present invention;

[0030] Figure 4 This is a schematic diagram of the internal structure of the detection card of the present invention;

[0031] Figure 5 This is a schematic diagram of the front structure of the detection card of the present invention;

[0032] Figure 6 This is a schematic diagram of the back structure of the detection card of the present invention;

[0033] Figure 7 It is a schematic diagram of the internal structure of the present invention;

[0034] Figure 8 It is a schematic diagram of the structure of the optical fiber collection of the present invention;

[0035] Fig. 9 It is a schematic diagram of the internal structure of the collecting optical fiber of the present invention;

[0036] Fig.10 It is a schematic diagram of the coupling component structure of the present invention;

[0037] Fig.11 It is a schematic diagram of the structure of the optical fiber connector of the present invention;

[0038] Fig.12 It is a circuit connection schematic diagram of the present invention;

[0039] Fig.13 It is a connection diagram of the light source driving circuit of the present invention;

[0040] Fig.14 It is a connection diagram of the amplifying and filtering circuit of the present invention;

[0041] Fig.15 It is a connection diagram of the A / D conversion circuit of the present invention.

[0042] In the figure: 1-body, 101-LCD screen, 102-detection entrance, 103-optical fiber interface, 2-fishtail handle, 201-keyboard, 202-battery box, 3-thermal printer, 4-detection card, 401-detection board, 402-test strip, 403-sample pad, 4011-concave table, 4012-suspended table, 4013-support plate, 4014-sample hole, 4015-rack, 4021-detection belt, 4022-control belt, 4023-QR code, 5-detection channel, 501-top observation window, 502-bottom transmission gap, 6-scanning transmission module, 601-transmission gear, 602-stepping motor, 603- micro switch, 604- roller, 7- light source channel, 701- light source, 702- light source filter, 8- light source feedback channel, 801- first plano-convex lens, 802- dichroic mirror, 803- detection filter, 804- second plano-convex lens, 805- grating, 806- spectrum meter, 9- circuit board, 10- coupling component, 101- first convex lens, 102- narrow band filter, 103- second convex lens, 11- collecting optical fiber, 1101- vertical incident optical fiber, 1102- horizontal receiving optical fiber, 1103- aggregation end, 12- spectroscope, 13- optical fiber bundle, 14- connector, 15- optical fiber probe. DETAILED DESCRIPTION

[0043] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0044] like Figure 1 , Figure 2 , Figure 3 As shown, a spectrum analysis device includes a body 1, a thermal printer 3 installed on the top of the body 1 and a fishtail handle 2 installed on the bottom of the body 1, wherein: the body 1 is a hollow rectangular parallelepiped structure, a liquid crystal screen 101 for displaying data is arranged on the front of the body 1, and a detection entrance 102 for placing an object to be detected and a fiber optic interface 103 for connecting an optical fiber bundle 13 are arranged on the side of the body 1; a keyboard 201 for information interaction is arranged on the front of the fishtail handle 2, and a battery box 202 for placing batteries is arranged on the back of the body 1; and also includes an optical fiber bundle 13 detachably connected to the optical fiber interface 103, one end of the optical fiber bundle 13 is connected to the aggregation end 1103 of the collecting optical fiber 11 through a connector 14, and the other end of the optical fiber bundle 13 is connected to the optical fiber probe 15 through the connector 14.

[0045] Specifically, this embodiment has an external optical fiber interface 103, which can directly analyze the reaction area outside the analyzer through the optical fiber bundle 13, connector 14 and optical fiber probe 15, which is particularly suitable for rapid detection under marine conditions. At the same time, through the reasonable design of the optical path, the light source and the spectrum measuring device are shared.

[0046] like Figure 4 As shown, the detection card 4 for inserting into the detection entrance 102 is also included, and the detection card 4 includes a detection plate 401, a test strip 402 placed on the detection plate 401, and a sample pad 403 arranged between the detection plate 401 and the test strip 402, wherein: the front side of the detection plate 401 is concave downward to form a concave platform 4011 for holding the test strip 402, as shown in FIG. Figure 5 The back of the detection plate 401 is provided with a rack 4015, as shown Figure 6 As shown; one end of the detection plate 401 is erected and then folded inward to form a suspended platform 4012, and the other end of the detection plate 401 is erected to form a support plate 4013 with the same height as the suspended platform 4012, and the suspended platform 4012 is located on the top of the sample loading pad 403, and a sample loading hole 4014 is opened on the suspended platform 4012.

[0047] Specifically, the height of the suspended platform 4012 and the support plate 4013 should be consistent and slightly lower than the height in the detection channel 5 to ensure that the detection card 4 can move smoothly and without shaking in the detection channel 5. A roller 604 for reducing the friction between the detection channel 5 and the detection card 4 is installed at the bottom of the detection channel 5. In order to further reduce the friction between the detection channel 5 and the detection card 4, the detection card 4 and the detection channel 5 can be made of a material with a low friction coefficient, such as nylon material.

[0048] Specifically, the test paper is provided with a detection band 4021 for capturing the antigen-antibody complex, a control band 4022 for enriching the free fluorescent marker, and a QR code 4023 for recording the serial number.

[0049] Test process example: First, the detection buffer (CRP antibody 1 labeled with a fluorescent marker) and the blood sample are mixed, and the CRP antibody 1 in the detection buffer reacts with the CRP (antigen) in the blood to form an antigen-antibody complex. When the mixture sample passes through the sample addition hole 4014 and the test strip 402 and is immersed in the test strip 402, it will quickly migrate on the test strip 402 through capillary action, and the CRP antigen-antibody complex is captured by the CRP antibody 2 fixed on the detection band 4021, enriched or trapped on the detection band 4021 of the test strip 402, while the free fluorescent marker will cross the detection band 4021 and be enriched at the control band 4022. The more CRP in the blood sample, the more complexes accumulate on the detection band 4021, and the content of the fluorescent substance on the detection band 4021 has a certain corresponding relationship with the CRP concentration.

[0050] like Figure 7 As shown, the detection entrance 102 extends into the interior of the fuselage 1 to form a detection channel 5, the top of the detection channel 5 is provided with an observation window 501, and the bottom of the detection channel 5 is provided with a transmission gap 502; the interior of the fuselage 1 is also provided with a scanning transmission module 6 for driving the detection card 4 to move, and the scanning transmission module 6 includes a transmission gear 601, a stepping motor 602 that drives the transmission gear 601 to rotate, and a micro switch 603 that senses the position of the detection card 4, the transmission gear 601 is arranged in the transmission gap 502 to drive the detection card 4 to move repeatedly through the meshing rack 4015, and the micro switch 603 is arranged at the bottom of the detection channel 5.

[0051] It should be noted that the stepper motor 602 is a 6.5MM two-phase four-wire stepper motor 602. According to actual conditions, two or more groups of stepper motors 602 can be set to jointly drive the detection card 4 to move.

[0052] Specifically, the outer contour of the detection card 4 is slightly smaller than the outer contour of the detection channel 5. When the user puts the detection card 4 to be detected into the detection channel 5, the bottom rack of the detection card 4 meshes with the transmission gear 601 at the bottom of the detection channel 5. When the detection card 4 is completely inserted into the detection channel 5, the detection card 4 will press the micro switch 603 at the bottom of the detection channel 5. At this time, the micro switch 603 will feedback the trigger information to the main control chip. After the main control chip obtains the information that the detection card 4 is fully inserted, it starts to control the stepper motor 602 to rotate forward and reverse to realize repeated scanning of the detection card 4.

[0053] like Figure 7As shown, a light source channel 7 for generating a light source is further provided inside the fuselage 1, the light source channel 7 is parallel to the detection channel 5, and includes a coaxially arranged light source 701, and a light source filter 702 located on one side of the light source 701; a light source feedback channel 8 for receiving feedback fluorescence is further provided inside the fuselage 1, the light source feedback channel 8 is perpendicular to the detection channel 5, and includes a coaxially arranged first plano-convex lens 801, a detection filter 803, a second plano-convex lens 804, a grating 805 and a spectrometer 806, and a dichroic mirror 802 inclined at 45° for light splitting is provided at the intersection of the axes of the detection channel 5 and the light source 701 channel 7, wherein: the first plano-convex lens 801 is installed on the top of the observation window 501, The dichroic mirror 802 is located between the first plano-convex lens 801 and the detection filter 803, and is located on the same side of the light source 701 as the light source filter 702. The spectrum measuring device 806 is used to measure the value of the received feedback light. The fuselage 1 also includes a collecting optical fiber 11, a spectroscope 12 and two sets of coupling components 10, wherein: the collecting optical fiber 11 includes a vertical incident optical fiber 1101 for collecting the light source 701, a horizontal receiving optical fiber 1102 for deriving the feedback light, and a collection end 1103 for collecting the vertical incident optical fiber 1101 and the horizontal receiving optical fiber 1102; a coupling component 10 is arranged between the vertical incident optical fiber 1101 and the light source 701; a coupling component 10 is also arranged between the horizontal receiving optical fiber 1102 and the dichroic mirror 802; and the collection end 1103 is installed in the optical fiber interface 103.

[0054] Specifically, the grating 805 is a pinhole grating 805 .

[0055] Specifically, the horizontal receiving optical fiber 1102 is coaxially arranged with the coupling component 10, and the vertical incident optical fiber 1101 is coaxially arranged with the coupling component 10 and the light source 701, and the extension lines of the horizontal receiving optical fiber 1102 and the vertical incident optical fiber 1101 are perpendicular to each other.

[0056] The specific structure of the optical fiber 11 is as follows: Figure 8 , Fig. 9 As shown, since the output light spot of the vertical incident optical fiber 1101 is circularly symmetrical, the obtained fluorescent light spot is also circularly symmetrical. Therefore, a symmetrically distributed optical fiber bundle 13 is used to receive the reflected light intensity to improve the efficiency and sensitivity of the received light. In order to reduce the loss during the transmission process, the optical fiber used is relatively short, about 60-80cm.

[0057] The simplest method of coupling the light source 701 with the optical fiber is direct coupling, that is, aligning the flat end face of the optical fiber directly with the light-emitting surface of the light source 701. This method is simple and convenient, but the coupling efficiency is low. If the numerical aperture of the optical fiber is 0.26, the maximum efficiency of direct coupling is only 6.7%. By placing a converging lens between the light source 701 and the end face of the optical fiber, changing the direction of the pulse xenon lamp or the receiving angle of the optical fiber, the coupling efficiency can be improved, such as Fig.10 As shown, the coupling assembly 10 includes a first convex lens, a narrow-band filter, and a second convex lens arranged coaxially. The coupling lens can collimate the excitation light beam to improve the injection efficiency of the probe, and can collect the fluorescence signal to improve the utilization rate of the fluorescence signal. The structural schematic diagram of the optical fiber connector 14 in this embodiment is shown in 11.

[0058] Specifically, the connector 14 can connect the multimode plastic optical fiber bundle 13, the single-mode optical fiber and the optical fiber probe 15, so as to generate a transmission path for the fluorescent signal and the excitation light. Specifically, the optical fiber bundle 13 can adopt an armored small soft optical cable according to the situation.

[0059] It should be noted that the working process of the light source 701 channel 7 and the light source feedback channel is as follows:

[0060] 1. Use test card 4 for measurement and analysis

[0061] During detection, the light source 701 is turned on, and the excitation light passes through the collimating filter and is focused by the 45° placed dichroic mirror 802 for splitting, and then by the focusing / collimating first plano-convex lens 801 onto the surface of the test strip 402. At this time, the stepper motor 602 drives the test strip 402 to move smoothly. The excitation light scans the detection area on the test strip 402. The fluorescence excited by the fluorescent substance in the detection area on the test strip 402 is collimated into parallel light by the first plano-convex lens 801, and the stray light is filtered out by the dichroic mirror 802 and the detection filter 803, and then focused on the confocal pinhole aperture by the second plano-convex lens 804. The light passing through the aperture is detected by the spectrometer 806 and converted into an electrical signal, which can be converted into the concentration of the sample to be tested after processing, analysis and calculation.

[0062] 2. Measurement and analysis using fiber optic probe 15

[0063] During detection, the light source 701 is turned on, and the excitation light enters the vertical incident optical fiber 1101 through the coupling component 10, and then enters the optical fiber probe 15 through the collection end 1103, the connector 14, the optical fiber bundle 13, and the connector 14 in sequence, and the optical fiber probe 15 emits the excitation light; the fluorescence excited by the fluorescent substance in the detection area is then fed back to the horizontal receiving optical fiber 1102 through the connector 14, the optical fiber bundle 13, the connector 14, and the collection end 1103 in the optical fiber probe 15 in sequence. At this time, the fluorescence fed back by the horizontal receiving optical fiber 1102 changes the incident direction through the spectroscope 12, and the light passing through the aperture is detected by the spectrum measuring device 806 and converted into an electrical signal, which can be converted into the concentration of the sample under test after processing, analysis and calculation.

[0064] like Fig.12 As shown, a circuit board is also provided in the fuselage 1, and the circuit board includes a main control chip, an amplifying and filtering circuit, a spectrum measuring device 806, a light source 701 driving circuit, and an ID chip, a power supply circuit, an A / D conversion circuit, a timing driving circuit, a synchronous control circuit and a motor driving circuit respectively connected to the main control chip, wherein: the main control chip is respectively connected to the LCD screen 101, the keyboard 201 and the thermal printer 3; the main control chip obtains the information of the spectrum measuring device 806 through the A / D conversion circuit and the amplifying and filtering circuit in turn; the spectrum measuring device 806 is connected to the timing driving circuit; the main control chip drives the light source 701 to work through the synchronous control circuit and the light source 701 driving circuit in turn; the main control chip drives the stepping motor 602 to control through the click driving circuit; the main control chip is connected to the positive and negative poles in the battery box 202 through the power supply circuit.

[0065] In this embodiment, the light source 701 may be a xenon lamp light source 701 .

[0066] Fig.13 The diagram of the connection of the driving circuit of the light source 701 of the present invention is shown in FIG. The energy storage capacitor C is charged by the power supply. When the voltage at both ends of the capacitor C reaches a preset value, the voltage stabilizing control element starts to work to keep the voltage constant. When flashing is required, the main control chip controls the trigger circuit through the interface circuit, and the capacitor E is quickly discharged through the resistor R1 to generate a strong current. The trigger pulse transformer generates a high-voltage electric pulse of tens of thousands of volts at its secondary stage, which ionizes the gas in the xenon lamp and greatly reduces the internal resistance of the lamp. The large amount of electric energy stored in the energy storage capacitor C is released through the pulse xenon lamp in a very short time, thereby generating an extremely strong flash.

[0067] This embodiment uses the CA3450 operational amplifier for reverse amplification. Although the CA3450 completes the reverse amplification of the signal, the noise in its output signal is relatively large, and a filter circuit is required to filter out the noise to improve the signal quality. Because the signal frequency to be obtained is relatively low, a commonly used voltage-controlled voltage source second-order low-pass filter is selected to filter out the noise, such as Fig.14shown.

[0068] The sampling and holding stage of this embodiment is mainly realized by a sample / hold device, while the quantization and encoding stage is mainly realized by an A / D converter. The A / D converter of this embodiment uses MAX120. Gain adjustment and bipolar offset adjustment are performed by Fig.15 The adjustment is realized by the potentiometers RP2 and RP1 in the A / D converter. The offset adjustment should be made before the gain adjustment. There may be some mutual influence between the two adjustments, and repeated adjustments are required. The adjustment of offset and gain is a subdivision of A / D conversion, and its purpose is to improve the accuracy of A / D.

[0069] The spectrum measuring device 806 of this embodiment may adopt a TCD1200D CCD or a silicon photocell.

[0070] In this embodiment, C-reactive protein was detected. Within the reagent concentration range of 2.00 to 30.00 mg / L, the same fluorescent reagent was selected at a concentration level of 2 mg / L for testing and analysis. Each concentration level was tested 4 times and the average value was taken as the final result. The measured results were subjected to linear regression analysis, and the obtained linear regression equation was: y=0.762x+0.215, where the correlation coefficient R 2 =0.987. The above results show that the instrument has good linear response characteristics in the concentration range of 2.00~30.00 mg / L.

[0071] The spectral analysis device provided in this embodiment has a compact structure, is portable and easy to use, and analyzes and detects human blood and urine based on the principles of fluorescent immunoassay and immunochromatography technology, solving the technical problems of complex mechanical transmission structure and low utilization rate of optical path components in existing analyzers.

[0072] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A spectrum analysis device, characterized in that: The device comprises a body, a thermal printer mounted on the top of the body and a fishtail handle mounted on the bottom of the body, wherein: the body is a hollow rectangular parallelepiped structure, a liquid crystal screen for displaying data is arranged on the front of the body, a detection entrance for placing the object to be inspected and an optical fiber interface for connecting the optical fiber bundle are arranged on the side of the body; a keyboard for information interaction is arranged on the front of the fishtail handle, and a battery box for placing batteries is arranged on the back of the body; It also includes a detection card for inserting into the detection entrance, the detection card includes a detection plate, a test strip placed on the detection plate, and a sample adding pad arranged between the detection plate and the test strip, wherein: the front side of the detection plate is recessed downward to form a concave platform for holding the test strip, a transmission rack is arranged on the back side of the detection plate, one end of the detection plate is erected and then folded inward by 90° to form a suspended platform, the other end of the detection plate is erected to form a support plate with the same height as the suspended platform, the suspended platform is located on the top of the sample adding pad, and a sample adding hole is opened on the suspended platform; The detection entrance extends toward the inside of the fuselage to form a detection channel, an observation window is provided at the top of the detection channel, and a transmission notch is provided at the bottom of the detection channel; A scanning transmission module for driving the detection card to move is also arranged inside the fuselage, and the scanning transmission module includes a transmission gear, a stepping motor for driving the transmission gear to rotate, and a micro switch for sensing the position of the detection card. The transmission gear is arranged in the transmission gap to drive the detection card to move repeatedly by engaging with the transmission rack, and the micro switch is arranged at the bottom of the detection channel; The fuselage is also provided with a light source channel for generating a light source, the light source channel is parallel to the detection channel and includes a coaxially arranged light source and a light source filter located on one side of the light source; The fuselage is also provided with a light source feedback channel for receiving feedback fluorescence, the light source feedback channel is perpendicular to the detection channel, and includes a first plano-convex lens, a detection filter, a second plano-convex lens, a grating and a spectrum measuring device which are coaxially arranged, and a dichroic mirror inclined at 45° for light splitting is arranged at the intersection of the axis of the light source feedback channel and the axis of the light source channel, wherein: the first plano-convex lens is installed on the top of the observation window, the dichroic mirror is located between the first plano-convex lens and the detection filter, and is located on the same side of the light source as the light source filter, and the spectrum measuring device is used to measure the value of the received feedback light; The fuselage also includes a collecting optical fiber, a beam splitter and two sets of coupling components, wherein: the collecting optical fiber includes a vertical incident optical fiber for collecting the light source, a horizontal receiving optical fiber for deriving feedback light, and a collection end for collecting the vertical incident optical fiber and the horizontal receiving optical fiber; a coupling component is arranged between the vertical incident optical fiber and the light source; a coupling component is also arranged between the horizontal receiving optical fiber and the dichroic mirror; the collection end is installed in the optical fiber interface; The stepper motor is a 6.5MM two-phase four-wire stepper motor.

2. The spectrum analysis device according to claim 1, characterized in that: A roller for reducing the friction between the detection channel and the detection card is installed at the bottom of the detection channel.

3. The spectrum analysis device according to claim 2, characterized in that: The aggregation end includes a parallel incident optical fiber located at the center and a plurality of parallel receiving optical fibers uniformly surrounding the parallel incident optical fiber.

4. The spectrum analysis device according to claim 3, characterized in that: The aggregation end also includes a vertical incident optical fiber located in the center and parallel receiving optical fibers uniformly surrounding the outside of the vertical incident optical fiber.

5. The spectrum analysis device according to claim 4, characterized in that: It also includes an optical fiber bundle detachably connected to the optical fiber interface, one end of the optical fiber bundle is connected to the aggregation end of the collected optical fibers through a connector, and the other end of the optical fiber bundle is connected to the optical fiber probe through a connector.

6. The spectrum analysis device according to claim 5, characterized in that: The coupling assembly includes a first convex lens, a narrow-band filter and a second convex lens which are coaxially arranged.

7. The spectrum analysis device according to claim 6, characterized in that: The test paper is provided with a detection band for capturing antigen-antibody complexes, a control band for enriching free fluorescent markers, and a two-dimensional code for recording serial numbers.

8. The spectrum analysis device according to claim 7, characterized in that: The fuselage is also provided with a circuit board, which includes a main control chip, an amplifying and filtering circuit, a spectrum measuring device, a light source driving circuit, and an ID chip, a power supply circuit, an A / D conversion circuit, a timing driving circuit, a synchronous control circuit and a motor driving circuit respectively connected to the main control chip, wherein: The main control chip is respectively connected to the LCD screen, keyboard and thermal printer; The main control chip obtains the spectrum measuring device information through the A / D conversion circuit and the amplification and filtering circuit in sequence; The spectrum measuring device is connected to the timing driving circuit; The main control chip drives the light source to work through the synchronous control circuit and the light source driving circuit in sequence; The main control chip drives the stepper motor control by clicking the drive circuit; The main control chip is connected to the positive and negative electrodes in the battery box through a power circuit.

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