A centrifugal microfluidic chip capable of realizing automatic sealing of liquid by beads

Abstract

The present invention provides a structure for realizing automatic sealing of liquid by balls, so as to solve the problem of switching between the state of having balls blocking liquid channels and the state of having balls blocking liquid channels in sample detection. When the balls block the liquid channels, reactants exist in the liquid storage cavity, which is convenient for realizing biochemical reactions. When the balls do not block the liquid channels, the liquid storage cavity is connected to the liquid channel, which is convenient for discharging the liquid in the liquid storage cavity through centrifugal force.

Description

Technical Field

[0001] The invention relates to the field of sample detection and microfluidic chip, and in particular to a round bead automatic liquid blocking structure used for sample detection in a microfluidic chip. Background Art

[0002] Lab-on-a-chip or microfluidic chip technology integrates the processing, analysis and detection of chemical and biological samples onto a micrometer-scale fluid chip. The microchannels form a network that can control the flow of fluid throughout the entire system. It can achieve low-cost, rapid, efficient and high-throughput analysis and detection, and is an important information collection and processing platform for modern biological and chemical sciences. Microfluidic chips can not only complete automated operations, detection and analysis in traditional chemistry and biology, but can also smoothly implement certain experiments that are difficult or impossible to complete using traditional biological and chemical methods. Microfluidic chip technology has been widely used in the fields of pharmaceuticals, clinical diagnosis, food testing, environmental testing, etc., with its advantages of flexible combination and large-scale integration of various unit technologies on an overall controllable micro-platform. Summary of the invention

[0003] The present invention provides a structure for realizing automatic sealing of liquid by balls, so as to solve the problem of switching between the state of having balls blocking liquid channels and the state of having balls blocking liquid channels in sample detection. When the balls block the liquid channels, reactants exist in the liquid storage cavity, which is convenient for realizing biochemical reactions. When the balls do not block the liquid channels, the liquid storage cavity is connected to the liquid channel, which is convenient for discharging the liquid in the liquid storage cavity through centrifugal force.

[0004] The microfluidic chip for automatically blocking liquid centrifugal force with round beads provided by the present invention is mainly composed of a chip body, a rotating shaft and round beads. A liquid storage cavity and a liquid channel are arranged on the chip body.

[0005] In the present invention, the rotating shaft and the chip body are fixed by a fixing device. In order to realize the switching of the ball blocking channel, the rotating shaft rotates clockwise, and the instantaneous acceleration causes the ball to move to the arc-shaped concave surface B position in the liquid storage chamber, and the channel is in a closed state; the rotating shaft rotates counterclockwise, and the instantaneous acceleration causes the ball to move to the arc-shaped concave surface A position in the liquid storage chamber, and the channel is in an open state; by switching the rotation direction of the rotating shaft, the opening and closing of the ball blocking the liquid channel are completed.

[0006] The advantages of the present invention are: the production process of the present invention is relatively simple, the conversion speed is relatively fast, and the portable control of the sample on the chip body can be realized. BRIEF DESCRIPTION OF THE DRAWINGS

[0007] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation of the present invention. In the accompanying drawings:

[0008] 1. Figure 1 It is a plan view of the ball automatic plugging structure, in which 1 represents the rotating shaft, 2 represents the liquid storage chamber, the liquid storage chamber has two arc-shaped concave structures A and B, 3 represents the ball for plugging the liquid, the ball is located in the liquid storage chamber, and currently the ball is in the arc-shaped concave position A (channel closed).

[0009] 2. Figure 2 It is a side schematic diagram of the ball automatic blocking structure, in which 1 represents the rotating shaft, 2 represents the liquid storage chamber and two arc-shaped concave structures A and B, 3 represents the ball used to block the liquid, the ball is located in the liquid storage chamber, and the ball is currently in the arc-shaped concave position A (the channel is closed), and 4 represents the liquid channel connected to the liquid storage chamber.

[0010] 3. Figure 3 This is the application diagram of the ball automatic blocking structure Figure 1 / 2, in the figure, 1 represents the rotation axis, 2 represents the liquid storage chamber, which has two arc-shaped concave structures A and B, 3 represents the round ball used to block the liquid, and the round ball is currently in the arc-shaped concave position A (channel closed), 4 represents the liquid channel connecting the liquid storage chamber and the detection chamber, and 5 represents the detection chamber. In this schematic diagram, the liquid in the liquid storage chamber cannot enter the detection chamber through the liquid channel by centrifugal force.

[0011] 4. Figure 4 This is the application diagram of the ball automatic blocking structure Figure 2 / 2, the ball is currently in the arc-shaped concave position B (the channel is open). In this schematic diagram, the liquid in the liquid storage chamber can enter the detection chamber through the liquid channel through centrifugal force. DETAILED DESCRIPTION

[0012] The preferred embodiments of the present invention are described below in conjunction with the accompanying drawings. It should be understood that the implementation examples described herein are only used to illustrate and explain the present invention, and are not used to limit the present invention.

[0013] A simple ball-shaped automatic plugging liquid centrifugal force microfluidic chip structure includes a chip body, a chip rotation axis and a ball-shaped chip. The chip body is provided with a liquid storage cavity and a liquid channel. The specific structure diagram is shown in FIG. Figure 1 , Figure 2 As shown, the specific implementation method is as follows Figure 3 , Figure 4 As shown. Structure 1 is a rotating shaft, which is connected to a motor to control the rotation speed and direction of the chip and is fixed to the chip body. Structure 2 is a liquid storage cavity located on the chip body, which has two arc-shaped concave structures A and B. Structure 3 is a round ball for blocking liquid, which is stored in the liquid storage cavity. Structure 4 is a liquid channel connecting the liquid storage cavity and the detection cavity. The two ends of the channel are connected, and its cross-sectional area is smaller than the maximum cross-sectional area of ​​the round ball. Structure 5 represents the detection cavity, which is connected to the liquid channel.

[0014] Specific implementation of the ball-shaped plugging liquid operation process: Assume that there is reaction liquid in the liquid storage chamber.

[0015] 1. When the liquid in the liquid storage chamber is incubating, the rotating shaft rotates to give the chip body a counterclockwise acceleration. The chip body rotates with the rotating shaft. At the same time, the ball enters the arc-shaped concave area A of the liquid storage chamber to block the liquid channel connecting the liquid storage chamber and the detection chamber. At this time, the liquid is confined in the liquid storage chamber;

[0016] 2. When the liquid in the liquid storage chamber needs to enter the detection chamber, the acceleration direction of the rotating shaft is changed, and the chip body is given a clockwise rotation acceleration by rotating the rotating shaft. The chip body moves with the rotating shaft. At the same time, the ball enters the arc-shaped concave area B of the liquid storage chamber, and the liquid channel connecting the liquid storage chamber and the detection chamber is connected. At this time, as the rotation speed increases, the liquid will enter the detection chamber through the liquid channel after reaching a certain rotation speed.

[0017] It can be seen that if one wants to switch the state of the liquid blocked by the ball in the liquid storage chamber, one only needs to switch the rotation direction of the rotating shaft, which is convenient and quick, and can achieve multiple conversions.

Claims

1. A centrifugal microfluidic chip capable of realizing automatic sealing of liquid by balls, the microfluidic chip comprising a chip body, a rotating shaft and balls, a liquid storage cavity and a liquid channel being arranged on the chip body, the rotating shaft being perpendicular to the plane of the chip body, characterized in that: The chip body is also provided with one or more round ball automatic blocking structures, which include round balls and a "3"-shaped structure. The "3"-shaped structure is composed of two arcs arranged in parallel to form a cavity wall shaped like the number "3". The opening side of the "3"-shaped structure faces the rotation axis of the chip, forming the cavity wall of the liquid storage cavity away from the rotation axis. A small hole is set at the top of one of the arc-shaped concave surfaces A, and the small hole is connected to the liquid channel. The other arc-shaped concave surface B has no small hole at the top. The round ball can move in the two arc-shaped concave surfaces A and B that constitute the "3"-shaped structure. When the round ball is in the arc-shaped concave surface A, the liquid channel is blocked, and when the round ball is in the arc-shaped concave surface B, the liquid channel is open; the surface of the round ball is wrapped with rubber material; the small hole set at the top of the arc-shaped concave surface A is set in a funnel shape, and the round ball enters the funnel-shaped structure to seal the liquid more tightly.

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