Zwitter-ion modified hydrophilic carbon spheres and preparation thereof, and applications in glycopeptide enrichment

Abstract

The invention particularly relates to preparation of amphoteric ion modified hydrophilic carbon spheres, and applications of the amphoteric ion modified hydrophilic carbon spheres in glycopeptide enrichment. According to the invention, glucose is used as a carbon source, a choline phosphate type monomer is used as a functional reagent to provide zwitterions, and carbon spheres with a particle sizeof 200-400 nm and hydrophilic surfaces are prepared through a hydrothermal method, wherein the preparation process of the hydrophilic carbon spheres is simple, the reaction conditions are mild, and the used raw materials are environmentally friendly; and the enrichment performance of the zwitterionic hydrophilic carbon spheres on glycopeptides is inspected by using a standard glycoprotein IgG enzymatic hydrolysate, and the zwitterionic hydrophilic carbon spheres are further applied to glycoproteomics analysis in serum.

Description

technical field

[0001] The invention specifically relates to the preparation of zwitterion-modified hydrophilic carbon spheres, which can be used for glycopeptide enrichment in the field of life and health. Background technique

[0002] Protein glycosylation is one of the most important post-translational modifications, which occurs in more than half of human proteins according to the records and predictions of the Swiss-Prot database (literature 1.A.Bairoch et.al "The Swiss-ProtProtein Sequence Data Bank and Its Supplement Trembl", 1999). Glycoproteins have been shown to be involved in many important cellular activities and disease processes, such as cell communication, signaling and cell adhesion. More importantly, many glycoproteins have been considered as biomarkers and therapeutic targets in clinical research (literature 2.Y.Tian et.al "Solid-phase extraction of N-linked glycopeptides", "Nature Protocols", 2007, 2, 334). Therefore, the study of protein glycosylation ...

Examples

Embodiment 1

[0022] (1) Preparation of zwitterionic hydrophilic carbon spheres

[0023] Dissolve 0.25g of glucose and 0.25g of the zwitterionic monomer 2-methacryloyloxyethylphosphorylcholine in 2.5mL of water, and add the mixed solution to a 10mL reaction kettle equipped with a polytetrafluoroethylene liner. After heating at 180°C for 6 hours, the obtained product (HTC-Glc-MPC) was washed with water and ethanol in turn until the supernatant was colorless, and dried in vacuum at 60°C for 12 hours before use.

[0024] The control group does not add the zwitterionic monomer 2-methacryloyloxyethyl phosphorylcholine, and directly uses the same method to carry out the hydrothermal reaction with the same concentration of glucose solution, and the product (HTC-Glc) is washed and washed with the same method as above. drying method. The reaction mechanism is roughly as follows: during the hydrothermal process, glucose is dehydrated and carbonized and polymerized to produce carbon spheres with fura...

technical field

[0001] The invention specifically relates to the preparation of zwitterion-modified hydrophilic carbon spheres, which can be used for glycopeptide enrichment in the field of life and health. Background technique

[0002] Protein glycosylation is one of the most important post-translational modifications, which occurs in more than half of human proteins according to the records and predictions of the Swiss-Prot database (literature 1.A.Bairoch et.al "The Swiss-ProtProtein Sequence Data Bank and Its Supplement Trembl", 1999). Glycoproteins have been shown to be involved in many important cellular activities and disease processes, such as cell communication, signaling and cell adhesion. More importantly, many glycoproteins have been considered as biomarkers and therapeutic targets in clinical research (literature 2.Y.Tian et.al "Solid-phase extraction of N-linked glycopeptides", "Nature Protocols", 2007, 2, 334). Therefore, the study of protein glycosylation ...

Examples

Embodiment 1

[0022] (1) Preparation of zwitterionic hydrophilic carbon spheres

[0023] Dissolve 0.25g of glucose and 0.25g of the zwitterionic monomer 2-methacryloyloxyethylphosphorylcholine in 2.5mL of water, and add the mixed solution to a 10mL reaction kettle equipped with a polytetrafluoroethylene liner. After heating at 180°C for 6 hours, the obtained product (HTC-Glc-MPC) was washed with water and ethanol in turn until the supernatant was colorless, and dried in vacuum at 60°C for 12 hours before use.

[0024] The control group does not add the zwitterionic monomer 2-methacryloyloxyethyl phosphorylcholine, and directly uses the same method to carry out the hydrothermal reaction with the same concentration of glucose solution, and the product (HTC-Glc) is washed and washed with the same method as above. drying method. The reaction mechanism is roughly as follows: during the hydrothermal process, glucose is dehydrated and carbonized and polymerized to produce carbon spheres with fura...

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